{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:23:40Z","timestamp":1788909820732,"version":"build-2803163510"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.micpath.2023.106419","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T11:33:22Z","timestamp":1697801602000},"page":"106419","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Bacillus subtilis expressing duck Tembusu virus E protein induces immune protection in ducklings"],"prefix":"10.1016","volume":"185","author":[{"given":"Chong","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghao","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8844-8974","authenticated-orcid":false,"given":"Fangkun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2023.106419_bib1","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/j.1865-1682.2011.01275.x","article-title":"Analysis of the complete genome of Tembusu virus, a flavivirus isolated from ducks in China","volume":"59","author":"Tang","year":"2012","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.micpath.2023.106419_bib2","doi-asserted-by":"crossref","first-page":"2413","DOI":"10.1099\/jgv.0.000908","article-title":"An updated review of avian-origin Tembusu virus: a newly emerging avian Flavivirus","volume":"98","author":"Zhang","year":"2017","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.micpath.2023.106419_bib3","first-page":"79","article-title":"Research progress of duck Tambusu virus","volume":"34","author":"Ping","year":"2012","journal-title":"Chin. J. Prev. Vet. Med."},{"key":"10.1016\/j.micpath.2023.106419_bib4","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/tbed.12085","article-title":"Tembusu virus in human, China","volume":"60","author":"Tang","year":"2013","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.micpath.2023.106419_bib5","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.virol.2013.12.028","article-title":"Development of a live attenuated vaccine candidate against duck Tembusu viral disease","volume":"450\u2013451","author":"Li","year":"2014","journal-title":"Virology"},{"key":"10.1016\/j.micpath.2023.106419_bib6","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1637\/10960-101514-Reg","article-title":"Efficacy evaluation of an inactivated duck tembusu virus vaccine","volume":"59","author":"Lin","year":"2015","journal-title":"Avian Dis."},{"key":"10.1016\/j.micpath.2023.106419_bib7","doi-asserted-by":"crossref","DOI":"10.3390\/v10040180","article-title":"Oral vaccination with a DNA vaccine encoding capsid protein of duck tembusu virus induces protection immunity","volume":"10","author":"Huang","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2023.106419_bib8","article-title":"Oral delivery of a DNA vaccine expressing the PrM and E genes: a promising vaccine strategy against flavivirus in ducks","volume":"8","author":"Huang","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2023.106419_bib9","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1292\/jvms.18-0036","article-title":"A recombinant adenovirus expressing the E protein of duck Tembusu virus induces protective immunity in duck","volume":"81","author":"Tang","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.micpath.2023.106419_bib10","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1292\/jvms.20-0363","article-title":"Application of Bacillus subtilis as a live vaccine vector: a review","volume":"82","author":"Lv","year":"2020","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.micpath.2023.106419_bib11","first-page":"1135","article-title":"Bacillus subtilis comes of age as a vaccine production host and delivery vehicle","volume":"14","author":"Rosales-Mendoza","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.micpath.2023.106419_bib12","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.imlet.2019.10.013","article-title":"Mucosal vaccines: strategies and challenges","volume":"217","author":"Li","year":"2020","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.micpath.2023.106419_bib13","doi-asserted-by":"crossref","DOI":"10.1042\/BSR20191126","article-title":"Mucosal immune responses induced by oral administration recombinant Bacillus subtilis expressing the COE antigen of PEDV in newborn piglets","volume":"39","author":"Wang","year":"2019","journal-title":"Biosci. Rep."},{"key":"10.1016\/j.micpath.2023.106419_bib14","first-page":"13","article-title":"Rational vaccine design in times of emerging diseases: the critical choices of immunological correlates of protection, vaccine antigen and immunomodulation","author":"Schijns","year":"2021","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.micpath.2023.106419_bib15","article-title":"Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus","volume":"8","author":"Yu","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2023.106419_bib16","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s12985-020-01334-w","article-title":"Heterologous prime-boost: an important candidate immunization strategy against Tembusu virus","volume":"17","author":"Pan","year":"2020","journal-title":"Virol. J."},{"key":"10.1016\/j.micpath.2023.106419_bib17","doi-asserted-by":"crossref","first-page":"581","DOI":"10.3389\/fmicb.2015.00581","article-title":"Effect of age on the pathogenesis of duck tembusu virus in Cherry Valley ducks","volume":"6","author":"Li","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2023.106419_bib18","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.vetmic.2013.10.003","article-title":"Tembusu virus infection in Cherry Valley ducks: the effect of age at infection","volume":"168","author":"Sun","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2023.106419_bib19","article-title":"Oral delivery of a DNA vaccine expressing the PrM and E genes: a promising vaccine strategy against flavivirus in ducks","volume":"8","author":"Huang","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2023.106419_bib20","doi-asserted-by":"crossref","first-page":"10986","DOI":"10.1073\/pnas.0703766104","article-title":"Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination","volume":"104","author":"Nochi","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.micpath.2023.106419_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.cellimm.2021.104361","article-title":"The diverse roles of myeloid derived suppressor cells in mucosal immunity","volume":"365","author":"Ashkenazi-Preiser","year":"2021","journal-title":"Cell. Immunol."},{"key":"10.1016\/j.micpath.2023.106419_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2022.109632","article-title":"Oral administration of recombinant Bacillus subtilis expressing a multi-epitope protein induces strong immune responses against Salmonella Enteritidis","volume":"276","author":"Lv","year":"2023","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2023.106419_bib23","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1007\/s00436-009-1606-7","article-title":"Immunogenicity of self-adjuvanticity oral vaccine candidate based on use of Bacillus subtilis spore displaying Schistosoma japonicum 26 KDa GST protein","volume":"105","author":"Li","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.micpath.2023.106419_bib24","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11274-018-2531-7","article-title":"Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond","volume":"34","author":"Cui","year":"2018","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.micpath.2023.106419_bib25","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1186\/s12917-020-02468-3","article-title":"Bacillus subtilis spore vaccines displaying protective antigen induce functional antibodies and protective potency","volume":"16","author":"Oh","year":"2020","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.micpath.2023.106419_bib26","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s13071-018-2757-0","article-title":"Bacillus subtilis spore with surface display of paramyosin from Clonorchis sinensis potentializes a promising oral vaccine candidate","volume":"11","author":"Sun","year":"2018","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.micpath.2023.106419_bib27","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1186\/s12934-016-0532-5","article-title":"Heterologous expression of antigenic peptides in Bacillus subtilis biofilms","volume":"15","author":"Vogt","year":"2016","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.micpath.2023.106419_bib28","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00427-18","article-title":"A single mutation at position 156 in the envelope protein of tembusu virus is responsible for virus tissue tropism and transmissibility in ducks","volume":"92","author":"Yan","year":"2018","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2023.106419_bib29","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s12964-020-0514-4","article-title":"Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2","volume":"18","author":"Zhang","year":"2020","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.micpath.2023.106419_bib30","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1053\/j.gastro.2014.01.060","article-title":"Regulation of the immune system by the resident intestinal bacteria","volume":"146","author":"Kamada","year":"2014","journal-title":"Gastroenterology"},{"key":"10.1016\/j.micpath.2023.106419_bib31","article-title":"Bacillus subtilis vector based oral rabies vaccines induced potent immune response and protective efficacy in mice","volume":"14","author":"Zhang","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2023.106419_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/srep38403","article-title":"Immune responses induced by recombinant Bacillus subtilis expressing the hemagglutinin protein of H5N1 in chickens","volume":"6","author":"Mou","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2023.106419_bib33","doi-asserted-by":"crossref","first-page":"e1365","DOI":"10.1111\/tbed.14467","article-title":"Dynamics of cellular and humoral immune responses following duck Tembusu virus infection in ducks","volume":"69","author":"Thontiravong","year":"2022","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.micpath.2023.106419_bib34","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.cyto.2016.01.004","article-title":"Anti-arthritogenic effect of Saponin-1 by alteration of Th1\/Th2 cytokine paradigm in arthritic mice","volume":"79","author":"Pandey","year":"2016","journal-title":"Cytokine"},{"key":"10.1016\/j.micpath.2023.106419_bib35","doi-asserted-by":"crossref","first-page":"68","DOI":"10.7774\/cevr.2015.4.1.68","article-title":"Cellular immune response following pre-exposure and postexposure rabies vaccination by intradermal and intramuscular routes","volume":"4","author":"Venkataswamy","year":"2015","journal-title":"Clin Exp Vaccine Res"},{"key":"10.1016\/j.micpath.2023.106419_bib36","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1099\/vir.0.067280-0","article-title":"A poorly neutralizing IgG2a\/c response elicited by a DNA vaccine protects mice against Japanese encephalitis virus","volume":"95","author":"Chen","year":"2014","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.micpath.2023.106419_bib37","doi-asserted-by":"crossref","first-page":"2699","DOI":"10.4049\/jimmunol.178.5.2699","article-title":"Immunization of flavivirus West Nile recombinant envelope domain III protein induced specific immune response and protection against West Nile virus infection","volume":"178","author":"Chu","year":"2007","journal-title":"J. Immunol."},{"key":"10.1016\/j.micpath.2023.106419_bib38","article-title":"Immune response to dengue virus infection in pediatric patients in New Delhi, India--Association of viremia, inflammatory mediators and monocytes with disease severity","volume":"10","author":"Singla","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2023.106419_bib39","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1111\/j.1600-065X.2008.00697.x","article-title":"Interleukin-2 in the development and control of inflammatory disease","volume":"226","author":"Hoyer","year":"2008","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.micpath.2023.106419_bib40","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1128\/jvi.71.1.169-178.1997","article-title":"Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2","volume":"71","author":"Chow","year":"1997","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2023.106419_bib41","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0264-410X(90)90171-H","article-title":"Attenuation and immunogenicity in primates of vaccinia virus recombinants expressing human interleukin-2","volume":"8","author":"Flexner","year":"1990","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2023.106419_bib42","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.molimm.2020.05.006","article-title":"Immunization of mice against alpha, beta, and epsilon toxins of Clostridium perfringens using recombinant rCpa-b-x expressed by Bacillus subtilis","volume":"123","author":"Wang","year":"2020","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.micpath.2023.106419_bib43","doi-asserted-by":"crossref","first-page":"888","DOI":"10.3389\/fimmu.2018.00888","article-title":"Tuning the cytokine responses: an update on interleukin (IL)-4 and IL-13 receptor complexes","volume":"9","author":"Junttila","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2023.106419_bib44","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1615\/CritRevImmunol.v32.i1.30","article-title":"Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease","volume":"32","author":"Iyer","year":"2012","journal-title":"Crit. Rev. Immunol."},{"key":"10.1016\/j.micpath.2023.106419_bib45","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/6104054","article-title":"IL-10: a multifunctional cytokine in viral infections","volume":"2017","author":"Rojas","year":"2017","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.micpath.2023.106419_bib46","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1186\/s12985-018-1053-0","article-title":"Immunization with a suicidal DNA vaccine expressing the E glycoprotein protects ducklings against duck Tembusu virus","volume":"15","author":"Tang","year":"2018","journal-title":"Virol. J."},{"key":"10.1016\/j.micpath.2023.106419_bib47","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2019.108508","article-title":"The truncated E protein of DTMUV provide protection in young ducks","volume":"240","author":"Li","year":"2020","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2023.106419_bib48","doi-asserted-by":"crossref","DOI":"10.1155\/2019\/1016567","article-title":"A novel adjuvant \"sublancin\" enhances immune response in specific pathogen-free broiler chickens inoculated with newcastle disease vaccine","volume":"2019","author":"Liu","year":"2019","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.micpath.2023.106419_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101358","article-title":"Effects of Bacillus subtilis and Bacillus licheniformis on growth performance, immunity, short chain fatty acid production, antioxidant capacity, and cecal microflora in broilers","volume":"100","author":"Xu","year":"2021","journal-title":"Poultry Sci."},{"key":"10.1016\/j.micpath.2023.106419_bib50","doi-asserted-by":"crossref","first-page":"354","DOI":"10.3389\/fimmu.2017.00354","article-title":"Bacillus subtilis improves immunity and disease resistance in rabbits","volume":"8","author":"Guo","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2023.106419_bib51","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.imlet.2014.11.017","article-title":"Molecular cloning, tissue distribution, and immune function of goose TLR7","volume":"163","author":"Qi","year":"2015","journal-title":"Immunol. Lett."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401023004527?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401023004527?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:00:22Z","timestamp":1760112022000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401023004527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":51,"alternative-id":["S0882401023004527"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2023.106419","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bacillus subtilis expressing duck Tembusu virus E protein induces immune protection in ducklings","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2023.106419","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"106419"}}