{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T11:18:45Z","timestamp":1778757525434,"version":"3.51.4"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["number.F.4-2\/2006 (BSR)\/BL\/18-19\/0387"],"award-info":[{"award-number":["number.F.4-2\/2006 (BSR)\/BL\/18-19\/0387"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.micpath.2024.107138","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T16:31:53Z","timestamp":1732033913000},"page":"107138","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Gene silencing of Histidyl-tRNA synthetase in Leishmania donovani promastigotes inhibits parasite growth and reduces virulence: A comprehensive computational and in vitro study"],"prefix":"10.1016","volume":"198","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4365-5720","authenticated-orcid":false,"given":"Vikas","family":"Kushwaha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6822-4949","authenticated-orcid":false,"given":"Sandeep","family":"Saini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neena","family":"Capalash","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2024.107138_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.actatropica.2023.106867","article-title":"Evaluation of immunomodulatory potential of recombinant histidyl-tRNA synthetase (rLdHisRS) protein of Leishmania donovani as a vaccine candidate against visceral leishmaniasis","volume":"241","author":"Kushwaha","year":"2023","journal-title":"Acta Trop."},{"key":"10.1016\/j.micpath.2024.107138_bib2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s43141-022-00318-3","article-title":"Screening and identification of potential inhibitor for visceral leishmaniasis (VL) through computational analysis","volume":"20","author":"Shaslinah","year":"2022","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"10.1016\/j.micpath.2024.107138_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.molbiopara.2022.111510","article-title":"Aminoacyl-tRNA synthetase (AARS) as an attractive drug target in neglected tropical trypanosomatid diseases-Leishmaniasis, Human African Trypanosomiasis and Chagas disease","volume":"251","author":"Kushwaha","year":"2022","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.micpath.2024.107138_bib4","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00079-18","article-title":"Leishmania donovani parasites are inhibited by the benzoxaborole AN2690 targeting leucyl-tRNA synthetase","volume":"62","author":"Manhas","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.micpath.2024.107138_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2022.983245","article-title":"Diversification of aminoacyl-tRNA synthetase activities via genomic duplication","volume":"13","author":"Krahn","year":"2022","journal-title":"Front. Physiol."},{"key":"10.1016\/j.micpath.2024.107138_bib6","author":"Pang"},{"key":"10.1016\/j.micpath.2024.107138_bib7","author":"Veerasamy"},{"key":"10.1016\/j.micpath.2024.107138_bib8","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.biochi.2014.08.005","article-title":"Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases","author":"Koh","year":"2014","journal-title":"Biochimie"},{"key":"10.1016\/j.micpath.2024.107138_bib9","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.jmb.2010.01.051","article-title":"Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs","volume":"397","author":"Merritt","year":"2010","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.micpath.2024.107138_bib10","author":"Merritt"},{"key":"10.1016\/j.micpath.2024.107138_bib11","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0006-291X(02)00525-9","article-title":"Genomic organization, transcriptional mapping, and evolutionary implications of the human bi-directional histidyl-tRNA synthetase locus (HARS\/HARSL)","volume":"294","author":"O'Hanlon","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.micpath.2024.107138_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.100913","article-title":"Peculiarities of aminoacyl-tRNA synthetases from trypanosomatids","volume":"297","author":"Parrot","year":"2021","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.micpath.2024.107138_bib13","article-title":"A therapeutic approach against Leishmania donovani by predicting RNAi molecules against the surface protein, gp63","volume":"13","author":"Chowdhury","year":"2018","journal-title":"Curr. Bioinf."},{"key":"10.1016\/j.micpath.2024.107138_bib14","first-page":"65","article-title":"Identification of chemical compounds that inhibit the function of histidyl-tRNA synthetase from Pseudomonas aeruginosa","volume":"23","author":"Hu","year":"2018","journal-title":"SLAS Discov. Adv. Life Sci. R D"},{"key":"10.1016\/j.micpath.2024.107138_bib15","first-page":"18","article-title":"Cross-protective efficacy of immuno-stimulatory recombinant Brugia malayi protein HSP60 against the Leishmania donovani in BALB\/c mice","volume":"72","author":"Kushwaha","year":"2021","journal-title":"Biol. J. Int. Assoc. Biol. Stand."},{"key":"10.1016\/j.micpath.2024.107138_bib16","doi-asserted-by":"crossref","first-page":"E73","DOI":"10.3390\/biomedicines6030073","article-title":"siRNAs targeting growth factor receptor and anti-apoptotic genes synergistically kill breast cancer cells through inhibition of MAPK and PI-3 kinase pathways","volume":"6","author":"Kamaruzman","year":"2018","journal-title":"Biomedicines"},{"key":"10.1016\/j.micpath.2024.107138_bib17","article-title":"Small interfering RNAs are highly effective inhibitors of crimean-Congo hemorrhagic fever virus replication in vitro","volume":"25","author":"F\u00f6ldes","year":"2020","journal-title":"Mol. Basel Switz."},{"key":"10.1016\/j.micpath.2024.107138_bib18","doi-asserted-by":"crossref","first-page":"436","DOI":"10.4103\/0378-6323.181473","article-title":"Down-regulation of peroxin synthesis by silencing RNA (siRNA): a novel hypothesis for treatment of leishmaniasis","volume":"82","author":"Keighobadi","year":"2016","journal-title":"Indian J. Dermatol. Venereol. Leprol."},{"key":"10.1016\/j.micpath.2024.107138_bib19","doi-asserted-by":"crossref","DOI":"10.1128\/mSphereDirect.00340-17","article-title":"Genetic validation of Leishmania donovani lysyl-tRNA synthetase shows that it is indispensable for parasite growth and infectivity","volume":"2","author":"Chadha","year":"2017","journal-title":"mSphere"},{"key":"10.1016\/j.micpath.2024.107138_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006575","article-title":"Genetic manipulation of Leishmania donovani threonyl tRNA synthetase facilitates its exploration as a potential therapeutic target","volume":"12","author":"Chadha","year":"2018","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2024.107138_bib21","first-page":"4054","article-title":"A parasite rescue and transformation assay for antileishmanial screening against intracellular Leishmania donovani amastigotes in THP1 human acute monocytic leukemia cell line","author":"Jain","year":"2012","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.micpath.2024.107138_bib22","doi-asserted-by":"crossref","first-page":"990","DOI":"10.2478\/s11686-020-00225-y","article-title":"Immunolocalization of disorganized muscle protein-1 in different life stages of human lymphatic filariid, Brugia malayi","volume":"65","author":"Kushwaha","year":"2020","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.micpath.2024.107138_bib23","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2024.107138_bib24","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/pro.3290","article-title":"Clustal Omega for making accurate alignments of many protein sequences","volume":"27","author":"Sievers","year":"2018","journal-title":"Protein Sci. Publ. Protein Soc."},{"key":"10.1016\/j.micpath.2024.107138_bib25","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/bib\/bbn017","article-title":"MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences","volume":"9","author":"Kumar","year":"2008","journal-title":"Briefings Bioinf."},{"key":"10.1016\/j.micpath.2024.107138_bib26","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1093\/nar\/gkg520","article-title":"SWISS-MODEL: an automated protein homology-modeling server","volume":"31","author":"Schwede","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2024.107138_bib27","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1016\/j.bpj.2011.10.024","article-title":"Improving the physical realism and structural accuracy of protein models by a two-step atomic-level energy minimization","volume":"101","author":"Xu","year":"2011","journal-title":"Biophys. J."},{"key":"10.1016\/j.micpath.2024.107138_bib28","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility","volume":"30","author":"Morris","year":"2009","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.micpath.2024.107138_bib29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1758-2946-3-33","article-title":"Open Babel: an open chemical toolbox","volume":"3","author":"O'Boyle","year":"2011","journal-title":"J. Cheminf."},{"key":"10.1016\/j.micpath.2024.107138_bib30","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1758-2946-4-17","article-title":"Avogadro: an advanced semantic chemical editor, visualization, and analysis platform","volume":"4","author":"Hanwell","year":"2012","journal-title":"J. Cheminf."},{"key":"10.1016\/j.micpath.2024.107138_bib31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"10.1016\/j.micpath.2024.107138_bib32","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1107\/S0907444904011679","article-title":"PRODRG: a tool for high-throughput crystallography of protein-ligand complexes","volume":"60","author":"Sch\u00fcttelkopf","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.micpath.2024.107138_bib33","series-title":"Mol. Biol","first-page":"e250","article-title":"Chapter e13 - Protein Synthesis","author":"Clark","year":"2013"},{"key":"10.1016\/j.micpath.2024.107138_bib34","doi-asserted-by":"crossref","DOI":"10.3390\/biom10121625","article-title":"Recent development of aminoacyl-tRNA synthetase inhibitors for human diseases: a future perspective","volume":"10","author":"Kim","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.micpath.2024.107138_bib35","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/nmat2626","article-title":"Multimeric small interfering ribonucleic acid for highly efficient sequence-specific gene silencing","volume":"9","author":"Mok","year":"2010","journal-title":"Nat. Mater."},{"key":"10.1016\/j.micpath.2024.107138_bib36","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.jbiotec.2011.12.003","article-title":"RNAi mediated silencing of ATPase RNA helicase gene in adult filarial parasite Brugia malayi impairs in vitro microfilaria release and adult parasite viability","volume":"157","author":"Singh","year":"2012","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.micpath.2024.107138_bib37","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.molbiopara.2004.10.002","article-title":"A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei","volume":"139","author":"Alibu","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.micpath.2024.107138_bib38","doi-asserted-by":"crossref","first-page":"10150","DOI":"10.1093\/nar\/gks813","article-title":"Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei","volume":"40","author":"Alsford","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2024.107138_bib39","doi-asserted-by":"crossref","first-page":"14256","DOI":"10.1074\/jbc.M112.447441","article-title":"Inhibition of isoleucyl-tRNA synthetase as a potential treatment for human African Trypanosomiasis","volume":"288","author":"Cestari","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.micpath.2024.107138_bib40","doi-asserted-by":"crossref","first-page":"98","DOI":"10.4103\/0972-9062.263718","article-title":"Transient knockdown of Nucleoside transporter 4 gene expression as a therapeutic target in Leishmania major by antisense RNA: in vitro and in vivo studies","volume":"56","author":"Tohidi","year":"2019","journal-title":"J. Vector Borne Dis."},{"key":"10.1016\/j.micpath.2024.107138_bib41","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1186\/1471-2164-13-621","article-title":"Unusual domain architecture of aminoacyl tRNA synthetases and their paralogs from Leishmania major","volume":"13","author":"Gowri","year":"2012","journal-title":"BMC Genom."},{"key":"10.1016\/j.micpath.2024.107138_bib42","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nprot.2016.051","article-title":"Computational protein-ligand docking and virtual drug screening with the AutoDock suite","volume":"11","author":"Forli","year":"2016","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.micpath.2024.107138_bib43","first-page":"573","article-title":"Halofuginone - the multifaceted molecule","volume":"20","author":"Pines","year":"2015","journal-title":"Mol. Basel Switz."},{"key":"10.1016\/j.micpath.2024.107138_bib44","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.1111\/bph.15442","article-title":"Halofuginone, a promising drug for treatment of pulmonary hypertension","volume":"178","author":"Jain","year":"2021","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.micpath.2024.107138_bib45","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/polb.21634","article-title":"Scaling law for the radius of gyration of proteins and its dependence on hydrophobicity","volume":"47","author":"Hong","year":"2009","journal-title":"J. Polym. Sci., Part B: Polym. Phys."},{"key":"10.1016\/j.micpath.2024.107138_bib46","first-page":"3067","article-title":"Molecular docking and MD: mimicking the real biological process","volume":"8","author":"Sharma","year":"2023","journal-title":"Phys. Sci. Rev."},{"key":"10.1016\/j.micpath.2024.107138_bib47","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1007\/s00436-022-07532-5","article-title":"In silico screening, molecular dynamic simulations, and in vitro activity of selected natural compounds as an inhibitor of Leishmania donovani 3-mercaptopyruvate sulfurtransferase","volume":"121","author":"Kant","year":"2022","journal-title":"Parasitol. Res."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401024006053?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401024006053?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T10:31:30Z","timestamp":1778754690000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401024006053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":47,"alternative-id":["S0882401024006053"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2024.107138","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gene silencing of Histidyl-tRNA synthetase in Leishmania donovani promastigotes inhibits parasite growth and reduces virulence: A comprehensive computational and in vitro study","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2024.107138","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107138"}}