{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:16:18Z","timestamp":1786994178600,"version":"build-2736575974"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.micpath.2025.107440","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T02:51:21Z","timestamp":1740711081000},"page":"107440","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Characterization of novel phages KPA\u04241, KP149\u04241, and KP149\u04242 for lytic efficiency against clinical MDR Klebsiella pneumoniae infections"],"prefix":"10.1016","volume":"202","author":[{"given":"Shraddha S.","family":"Dandekar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinta","family":"Thanikkal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8883-3767","authenticated-orcid":false,"given":"Arti","family":"Londhe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankhudi","family":"Bhutada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ujjayni","family":"Saha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2841-0270","authenticated-orcid":false,"given":"Shubhankar","family":"Pawar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Samson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3965-7320","authenticated-orcid":false,"given":"Mahesh","family":"Dharne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8071-2731","authenticated-orcid":false,"given":"Sunil D.","family":"Saroj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9413-950X","authenticated-orcid":false,"given":"Santosh","family":"Koratkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2025.107440_bib1","first-page":"1","author":"Venkateswaran","year":"2023"},{"key":"10.1016\/j.micpath.2025.107440_bib2","series-title":"WHO Bacterial Priority Pathogens List 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance","year":"2024"},{"key":"10.1016\/j.micpath.2025.107440_bib3","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ijid.2018.01.011","article-title":"Global forecast of antimicrobial resistance in invasive isolates of Escherichia coli and Klebsiella pneumoniae","volume":"68","author":"Alvarez-Uria","year":"2018","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.micpath.2025.107440_bib4","article-title":"Global prevalence of nosocomial multidrug-resistant Klebsiella pneumoniae: a systematic review and meta-analysis","volume":"10","author":"Asri","year":"2021","journal-title":"Antibiotics"},{"key":"10.1016\/j.micpath.2025.107440_bib5","series-title":"Welcome Trust HM Gov","first-page":"1","article-title":"Tackling drug-resistant infections globally : final report and recommendations the review on","author":"O'Neill","year":"2016"},{"key":"10.1016\/j.micpath.2025.107440_bib6","doi-asserted-by":"crossref","DOI":"10.1186\/s43088-024-00470-4","article-title":"Overcoming Klebsiella pneumoniae antibiotic resistance: new insights into mechanisms and drug discovery","volume":"13","author":"Huy","year":"2024","journal-title":"Beni-Suef Univ J Basic Appl Sci"},{"key":"10.1016\/j.micpath.2025.107440_bib7","article-title":"Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy","volume":"11","author":"Podschun","year":"1998","journal-title":"Typing Methods, and Pathogenicity Factors"},{"key":"10.1016\/j.micpath.2025.107440_bib8","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.VMBF-0016-2015","article-title":"Mechanisms of antibiotic resistance","volume":"4","author":"Munita","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micpath.2025.107440_bib9","doi-asserted-by":"crossref","first-page":"162","DOI":"10.4292\/wjgpt.v8.i3.162","article-title":"Phage therapy: an alternative to antibiotics in the age of multi-drug resistance","volume":"8","author":"Lin","year":"2017","journal-title":"World J Gastrointest Pharmacol Ther"},{"key":"10.1016\/j.micpath.2025.107440_bib10","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2016.01292","article-title":"Bacteria vs. bacteriophages: parallel evolution of immune arsenals","volume":"7","author":"Shabbir","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.107440_bib11","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4161\/bact.1.2.15845","article-title":"Phage treatment of human infections","volume":"1","author":"Abedon","year":"2011","journal-title":"Bacteriophage"},{"key":"10.1016\/j.micpath.2025.107440_bib12","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofad051","article-title":"Application of phage therapy in a case of a chronic hip-prosthetic joint infection due to Pseudomonas aeruginosa: an Italian real-life experience and in vitro analysis","volume":"10","author":"Cesta","year":"2023","journal-title":"Open Forum Infect. Dis."},{"key":"10.1016\/j.micpath.2025.107440_bib13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ijid.2022.04.049","article-title":"Case Report: successful use of phage therapy in refractory MRSA chronic rhinosinusitis","volume":"121","author":"Rodriguez","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.micpath.2025.107440_bib14","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2024.1397447","article-title":"Physiochemical characterization of a potential Klebsiella phage MKP-1 and analysis of its application in reducing biofilm formation","volume":"15","author":"Das","year":"2024","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.micpath.2025.107440_bib15","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/s41522-024-00603-8","article-title":"A Klebsiella-phage cocktail to broaden the host range and delay bacteriophage resistance both in vitro and in vivo","volume":"10","author":"Chen","year":"2024","journal-title":"NPJ Biofilms Microbiomes"},{"key":"10.1016\/j.micpath.2025.107440_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijmmb.2023.100515","article-title":"Effect of Klebsiella-specific phage on multidrug-resistant Klebsiella pneumoniae- an experimental study","volume":"47","author":"Shree","year":"2024","journal-title":"Indian J. Med. Microbiol."},{"issue":"12","key":"10.1016\/j.micpath.2025.107440_bib17","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.3390\/ph16121638","article-title":"Phage therapy-challenges, opportunities and future prospects","volume":"16","author":"Zalewska-Pi\u0105tek","year":"2023","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.micpath.2025.107440_bib18","volume":"vol. 41","author":"Dashti","year":"2009"},{"key":"10.1016\/j.micpath.2025.107440_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98457-z","article-title":"Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa","volume":"11","author":"Sharma","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.107440_bib20","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-37065-5","article-title":"Characterization of bacteriophage vB_KleM_KB2 possessing high control ability to pathogenic Klebsiella pneumoniae","volume":"13","author":"Peng","year":"2023","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.107440_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0127606","article-title":"Isolation of phages for phage therapy: a comparison of spot tests and efficiency of plating analyses for determination of host range and efficacy","volume":"10","author":"Mirzaei","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.107440_bib22","article-title":"Characterization, taxonomic classification, and genomic analysis of two newly isolated bacteriophages with potential to infect Escherichia coli","volume":"20","author":"Nazir","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micpath.2025.107440_bib23","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2025.107440_bib25","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1093\/bioinformatics\/btx157","article-title":"ViPTree: the viral proteomic tree server","volume":"33","author":"Nishimura","year":"2017","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2025.107440_bib26","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1000721","article-title":"Determination of phage susceptibility as a clinical diagnostic tool: a routine perspective","volume":"12","author":"Daubie","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.micpath.2025.107440_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/antibiotics10060710","article-title":"Bacteriophages with potential to inactivate Aeromonas hydrophila in Cockles: In vitro and in vivo preliminary studies","volume":"10","author":"Duarte","year":"2021","journal-title":"Antibiotics"},{"key":"10.1016\/j.micpath.2025.107440_bib28","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global spread of carbapenemase producing Enterobacteriaceae","volume":"17","author":"Nordmann","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.micpath.2025.107440_bib29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jiph.2020.12.001","article-title":"Molecular characterization of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from king abdulaziz specialist hospital at taif city, Saudi arabia","volume":"14","author":"Lagha","year":"2021","journal-title":"J Infect Public Health"},{"key":"10.1016\/j.micpath.2025.107440_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2019.00574","article-title":"Therapeutic characterization and efficacy of bacteriophage cocktails infecting Escherichia coli, Klebsiella pneumoniae, and enterobacter species","volume":"10","author":"Manohar","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.107440_bib31","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01994-22","article-title":"Characterization of novel Klebsiella phage PG14 and its antibiofilm efficacy","volume":"10","author":"Mulani","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micpath.2025.107440_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0245354","article-title":"Klebsiella virus UPM2146 lyses multiple drug-resistant Klebsiella pneumoniae in vitro and in vivo","volume":"16","author":"Assafiri","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.107440_bib33","article-title":"In vitro activity, stability and molecular characterization of eight potent bacteriophages infecting carbapenem-resistant Klebsiella pneumoniae","volume":"15","author":"Baqer","year":"2023","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2025.107440_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-68702-y","article-title":"Characterization of extended-spectrum-\u03b2-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo","volume":"10","author":"Wintachai","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.107440_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/s41538-023-00245-8","article-title":"Positive and negative aspects of bacteriophages and their immense role in the food chain","volume":"8","author":"Ranveer","year":"2024","journal-title":"Npj Sci Food"},{"key":"10.1016\/j.micpath.2025.107440_bib36","doi-asserted-by":"crossref","DOI":"10.1590\/s2175-97902018000117093","article-title":"Phage therapy: progress in pharmacokinetics","volume":"54","author":"Qadir","year":"2018","journal-title":"Brazilian Journal of Pharmaceutical Sciences"},{"key":"10.1016\/j.micpath.2025.107440_bib37","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.897531","article-title":"Isolation and characterization of vB_kpnM_17-11, a novel phage efficient against carbapenem-resistant Klebsiella pneumoniae","volume":"12","author":"Bai","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.micpath.2025.107440_bib38","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.01870","article-title":"Newly isolated bacteriophages from the Podoviridae, Siphoviridae, and Myoviridae families have variable effects on putative novel Dickeya spp","volume":"8","author":"Alic","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.107440_bib39","doi-asserted-by":"crossref","DOI":"10.1186\/s12985-024-02306-0","article-title":"Phage vB_Ec_ZCEC14 to treat antibiotic-resistant Escherichia coli isolated from urinary tract infections","volume":"21","author":"Ismael","year":"2024","journal-title":"Virol. J."},{"key":"10.1016\/j.micpath.2025.107440_bib40","first-page":"146","article-title":"Isolation and characterization of virulent bacteriophages against Klebsiella pneumoniae of significant capsular types","volume":"25","author":"Gorodnichev","year":"2023","journal-title":"Extrem Med"},{"key":"10.1016\/j.micpath.2025.107440_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2022.198873","article-title":"Isolation and genomic characterization of a novel Autographiviridae bacteriophage IME184 with lytic activity against Klebsiella pneumoniae","volume":"319","author":"Li","year":"2022","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2025.107440_bib42","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.virs.2022.04.005","article-title":"Identification of a phage-derived depolymerase specific for KL47 capsule of Klebsiella pneumoniae and its therapeutic potential in mice","volume":"37","author":"Li","year":"2022","journal-title":"Virol. Sin."},{"key":"10.1016\/j.micpath.2025.107440_bib43","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1080\/1040841X.2021.1962803","article-title":"Bacteriophage endolysins against gram-positive bacteria, an overview on the clinical development and recent advances on the delivery and formulation strategies","volume":"48","author":"Ho","year":"2021","journal-title":"Crit. Rev. Microbiol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025001652?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025001652?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T03:18:27Z","timestamp":1741922307000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401025001652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":42,"alternative-id":["S0882401025001652"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107440","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of novel phages KPA\u04241, KP149\u04241, and KP149\u04242 for lytic efficiency against clinical MDR Klebsiella pneumoniae infections","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107440","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107440"}}