{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:30:03Z","timestamp":1788849003562,"version":"build-2803163510"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1801105"],"award-info":[{"award-number":["2021YFD1801105"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","award":["222102110375"],"award-info":[{"award-number":["222102110375"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.micpath.2025.107522","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:27:34Z","timestamp":1743640054000},"page":"107522","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Construction and characterization of a gE\/gI\/TK-gene-deleted recombinant pseudorabies virus variant expressing the GP5 of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and NADC30-like PRRSV"],"prefix":"10.1016","volume":"203","author":[{"given":"Hui-Hua","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Qing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Yao","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Peng","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan-Lan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Jie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2025.107522_bib1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1136\/vr.136.2.32","article-title":"Porcine reproductive and respiratory syndrome: clinical disease, pathology and immunosuppression","volume":"136","author":"Done","year":"1995","journal-title":"Vet. Rec."},{"key":"10.1016\/j.micpath.2025.107522_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0000526","article-title":"Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark","volume":"2","author":"Tian","year":"2007","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.107522_bib3","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2174\/1874357901711010059","article-title":"NADC30-Like porcine reproductive and respiratory syndrome in China","volume":"11","author":"Tian","year":"2017","journal-title":"Open Virol. J."},{"key":"10.1016\/j.micpath.2025.107522_bib4","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/tbed.12530","article-title":"Outbreak investigation of NADC30-like PRRSV in South-East China","volume":"63","author":"Li","year":"2016","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.micpath.2025.107522_bib5","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.virusres.2012.07.030","article-title":"Genomic sequence and virulence comparison of four Type 2 porcine reproductive and respiratory syndrome virus strains","volume":"169","author":"Brockmeier","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2025.107522_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/v14081638","article-title":"Pseudorabies virus: from pathogenesis to prevention strategies","volume":"14","author":"Zheng","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2025.107522_bib7","doi-asserted-by":"crossref","first-page":"363","DOI":"10.4142\/jvs.2013.14.3.363","article-title":"Emergence of virulent pseudorabies virus infection in northern China","volume":"14","author":"R","year":"2013","journal-title":"J. Vet. Sci."},{"key":"10.1016\/j.micpath.2025.107522_bib8","doi-asserted-by":"crossref","first-page":"5540","DOI":"10.1016\/j.vaccine.2016.09.048","article-title":"Commercial vaccines provide limited protection to NADC30-like PRRSV infection","volume":"34","author":"Bai","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2025.107522_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcp.2020.101605","article-title":"Construction and immunogenicity of a gE\/gI\/TK-deleted PRV based on porcine pseudorabies virus variant","volume":"53","author":"Zhao","year":"2020","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.micpath.2025.107522_bib10","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/0378-1135(94)90067-1","article-title":"Porcine reproductive and respiratory syndrome: temperature and pH stability of Lelystad virus and its survival in tissue specimens from viraemic pigs","volume":"42","author":"Bloemraad","year":"1994","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib11","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0378-1135(96)01322-3","article-title":"Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview","volume":"55","author":"Albina","year":"1997","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib12","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.tvjl.2007.07.032","article-title":"Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China","volume":"174","author":"Li","year":"2007","journal-title":"Vet. J."},{"key":"10.1016\/j.micpath.2025.107522_bib13","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.vetmic.2010.11.001","article-title":"Comparative genomic analysis of five pairs of virulent parental\/attenuated vaccine strains of PRRSV","volume":"149","author":"An","year":"2011","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib14","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1016\/j.vaccine.2007.03.031","article-title":"Impact of a modified-live porcine reproductive and respiratory syndrome virus vaccine intervention on a population of pigs infected with a heterologous isolate","volume":"25","author":"Cano","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2025.107522_bib15","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.vetmic.2009.04.013","article-title":"Function of PRRSV GP5 envelope protein by using pseudotyped virus","volume":"138","author":"Xia","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib16","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/tbed.13427","article-title":"Natural infection of a variant pseudorabies virus leads to bovine death in China","volume":"67","author":"Cheng","year":"2020","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.micpath.2025.107522_bib17","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.vetmic.2014.09.003","article-title":"Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China","volume":"174","author":"Luo","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib18","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3201\/eid1911.130177","article-title":"Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012","volume":"19","author":"An","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.micpath.2025.107522_bib19","doi-asserted-by":"crossref","first-page":"132","DOI":"10.7774\/cevr.2016.5.2.132","article-title":"An oral Aujeszky's disease vaccine (YS-400) induces neutralizing antibody in pigs","volume":"5","author":"Yang","year":"2016","journal-title":"Clin Exp Vaccine Res"},{"key":"10.1016\/j.micpath.2025.107522_bib20","doi-asserted-by":"crossref","first-page":"5733","DOI":"10.1016\/j.vaccine.2015.09.066","article-title":"Novel pseudorabies virus variant with defects in TK, gE and gI protects growing pigs against lethal challenge","volume":"33","author":"Rm","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2025.107522_bib21","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.vetmic.2015.11.022","article-title":"Pathogenicity and immunogenicity of a gE\/gI\/TK gene-deleted pseudorabies virus variant in susceptible animals","volume":"182","author":"Cong","year":"2016","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib22","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/BF01718588","article-title":"Construction of bovine herpesvirus-1 (BHV-1) recombinants which express pseudorabies virus (PRV) glycoproteins gB, gC, gD, and gE","volume":"141","author":"Otsuka","year":"1996","journal-title":"Arch. Virol."},{"key":"10.1016\/j.micpath.2025.107522_bib23","doi-asserted-by":"crossref","DOI":"10.3390\/v15020370","article-title":"Recombinant pseudorabies virus usage in vaccine development against swine infectious disease","volume":"15","author":"Zhou","year":"2023","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2025.107522_bib24","first-page":"99","article-title":"Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis--state of the art, June 1999","volume":"31","author":"Mettenleiter","year":"2000","journal-title":"Vet Res"},{"key":"10.1016\/j.micpath.2025.107522_bib25","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/978-1-4939-9210-2_9","article-title":"LeishGEdit: a method for rapid gene knockout and tagging using CRISPR-Cas9","volume":"1971","author":"Beneke","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.micpath.2025.107522_bib26","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/s41556-020-0472-5","article-title":"Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing","volume":"22","author":"Artegiani","year":"2020","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.micpath.2025.107522_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2022.109397","article-title":"A NADC30-like PRRSV causes serious intestinal infections and tropism in piglets","volume":"268","author":"Ma","year":"2022","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib28","doi-asserted-by":"crossref","first-page":"4775","DOI":"10.1093\/nar\/27.24.4775","article-title":"Use of the human EF-1alpha promoter for expression can significantly increase success in establishing stable cell lines with consistent expression: a study using the tetracycline-inducible system in human cancer cells","volume":"27","author":"Gopalkrishnan","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2025.107522_bib29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0300-9084(99)80041-8","article-title":"The role of homologous recombination processes in the repair of severe forms of DNA damage in mammalian cells","volume":"81","author":"Thacker","year":"1999","journal-title":"Biochimie"},{"key":"10.1016\/j.micpath.2025.107522_bib30","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"Ran","year":"2013","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.micpath.2025.107522_bib31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-1-4939-7169-5_3","article-title":"Building Cre knockin rat lines using CRISPR\/Cas9","volume":"1642","author":"Ma","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.micpath.2025.107522_bib32","article-title":"An easy and efficient inducible CRISPR\/Cas9 platform with improved specificity for multiple gene targeting","volume":"44","author":"Cao","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2025.107522_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2019.104652","article-title":"Recombinant pseudorabies virus expressing E2 of classical swine fever virus (CSFV) protects against both virulent pseudorabies virus and CSFV","volume":"173","author":"Tong","year":"2020","journal-title":"Antiviral Res"},{"key":"10.1016\/j.micpath.2025.107522_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109022","article-title":"A new strategy to develop pseudorabies virus-based bivalent vaccine with high immunogenicity of porcine circovirus type 2","volume":"255","author":"Wu","year":"2021","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107522_bib35","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1186\/s12985-017-0825-2","article-title":"ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro","volume":"14","author":"Leng","year":"2017","journal-title":"Virol. J."},{"key":"10.1016\/j.micpath.2025.107522_bib36","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.vetimm.2005.03.008","article-title":"Protective immunity induced by a recombinant pseudorabies virus expressing the GP5 of porcine reproductive and respiratory syndrome virus in piglets","volume":"106","author":"Qiu","year":"2005","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.micpath.2025.107522_bib37","series-title":"PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) ENVELOPE GP5 PROTEIN FAILS TO PROTECT PIGS AGAINST PRRSV","author":"Fernandez","year":"2003"},{"key":"10.1016\/j.micpath.2025.107522_bib38","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1023\/A:1007931322271","article-title":"Baculovirus expression of proteins of porcine reproductive and respiratory syndrome virus strain Olot\/91. Involvement of ORF3 and ORF5 proteins in protection","volume":"14","author":"Plana Duran","year":"1997","journal-title":"Virus Genes"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025002475?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025002475?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T03:00:53Z","timestamp":1762311653000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401025002475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":38,"alternative-id":["S0882401025002475"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107522","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Construction and characterization of a gE\/gI\/TK-gene-deleted recombinant pseudorabies virus variant expressing the GP5 of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and NADC30-like PRRSV","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107522","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107522"}}