{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T16:17:35Z","timestamp":1787674655872,"version":"build-2784847793"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32473024"],"award-info":[{"award-number":["32473024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402837"],"award-info":[{"award-number":["32402837"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515110264"],"award-info":[{"award-number":["2023A1515110264"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515140052"],"award-info":[{"award-number":["2022A1515140052"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"publisher","award":["KTP20240768"],"award-info":[{"award-number":["KTP20240768"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010226","name":"Department of Education of Guangdong Province","doi-asserted-by":"publisher","award":["2022ZDJS036"],"award-info":[{"award-number":["2022ZDJS036"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.micpath.2025.107648","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T11:22:05Z","timestamp":1745925725000},"page":"107648","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Neferine inhibits PRRSV infection by disrupting p65 nuclear translocation"],"prefix":"10.1016","volume":"205","author":[{"given":"Qinqin","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7136-5849","authenticated-orcid":false,"given":"Qiang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2025.107648_bib1","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1128\/JCM.01876-10","article-title":"Utility of a panviral microarray for detection of swine respiratory viruses in clinical samples","volume":"49","author":"Nicholson","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.micpath.2025.107648_bib2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetmic.2013.04.029","article-title":"PLGA nanoparticle entrapped killed porcine reproductive and respiratory syndrome virus vaccine helps in viral clearance in pigs","volume":"166","author":"Dwivedi","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107648_bib3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1297-9716-44-73","volume":"44","author":"Frydas","year":"2013","journal-title":"Vet. Res."},{"key":"10.1016\/j.micpath.2025.107648_bib4","doi-asserted-by":"crossref","first-page":"10448","DOI":"10.1128\/JVI.01117-14","article-title":"Modulation of CD163 expression by metalloprotease ADAM17 regulates porcine reproductive and respiratory syndrome virus entry","volume":"88","author":"Guo","year":"2014","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib5","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s11262-010-0507-2","article-title":"Accelerated evolution of PRRSV during recent outbreaks in China","volume":"41","author":"Song","year":"2010","journal-title":"Virus Genes"},{"key":"10.1016\/j.micpath.2025.107648_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2020.197980","article-title":"Molecular and cellular mechanisms for PRRSV pathogenesis and host response to infection","volume":"286","author":"An","year":"2020","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2025.107648_bib7","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.vetmic.2017.02.020","article-title":"Pathogenesis and control of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus","volume":"209","author":"Han","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107648_bib8","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev-animal-022114-111025","article-title":"Porcine reproductive and respiratory syndrome virus (PRRSV): pathogenesis and interaction with the immune system","volume":"4","author":"Lunney","year":"2016","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"10.1016\/j.micpath.2025.107648_bib9","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01052-21","article-title":"Porcine reproductive and respiratory syndrome virus infection upregulates negative immune regulators and T-cell exhaustion markers","volume":"95","author":"Chaudhari","year":"2021","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib10","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1080\/15548627.2023.2179844","article-title":"Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1\/p62-dependent selective autophagy to antagonize its antiviral activity","volume":"19","author":"Li","year":"2023","journal-title":"Autophagy"},{"key":"10.1016\/j.micpath.2025.107648_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/ijms222010994","article-title":"Anti-allergic and anti-inflammatory effects of neferine on RBL-2H3 cells","volume":"22","author":"Chiu","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.micpath.2025.107648_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.alkal.2018.07.001","article-title":"Bisbenzylisoquinoline alkaloids","volume":"81","author":"Weber","year":"2019","journal-title":"Alkaloids - Chem. Biol."},{"key":"10.1016\/j.micpath.2025.107648_bib13","doi-asserted-by":"crossref","first-page":"6304","DOI":"10.1007\/s12035-021-02554-z","article-title":"Neferine protects against brain damage in permanent cerebral ischemic rat associated with autophagy suppression and AMPK\/mTOR regulation","volume":"58","author":"Sengking","year":"2021","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.micpath.2025.107648_bib14","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.lfs.2018.02.032","article-title":"Pharmacological benefits of neferine - a comprehensive review","volume":"199","author":"Marthandam","year":"2018","journal-title":"Life Sci."},{"key":"10.1016\/j.micpath.2025.107648_bib15","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1002\/jmv.27117","article-title":"Inhibitory effect on SARS-CoV-2 infection of neferine by blocking Ca(2+) -dependent membrane fusion","volume":"93","author":"Yang","year":"2021","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008543","article-title":"TREM2 suppresses the proinflammatory response to facilitate PRRSV infection via PI3K\/NF-\u03baB signaling","volume":"16","author":"Zhu","year":"2020","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micpath.2025.107648_bib17","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01251-17","article-title":"Genotype 2 strains of porcine reproductive and respiratory syndrome virus dysregulate alveolar macrophage cytokine production via the unfolded protein response","volume":"92","author":"Chen","year":"2018","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00767-19","article-title":"Porcine reproductive and respiratory syndrome virus E protein degrades porcine cholesterol 25-hydroxylase via the ubiquitin-proteasome pathway","volume":"93","author":"Ke","year":"2019","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib19","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.3389\/fmicb.2017.01635","article-title":"Improved vaccine against PRRSV: current progress and future perspective","volume":"8","author":"Nan","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.107648_bib20","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01436-19","article-title":"The nsp2 hypervariable region of porcine reproductive and respiratory syndrome virus strain JXwn06 is associated with viral cellular tropism to primary porcine alveolar macrophages","volume":"93","author":"Song","year":"2019","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib21","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1099\/vir.0.014001-0","article-title":"Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections","volume":"90","author":"Jung","year":"2009","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.micpath.2025.107648_bib22","first-page":"279","article-title":"[Use of silicone oil for temporary tamponade in the treatment of retinal detachment with vitreoretinal retraction. 2. Aspects, prevention and treatment of complications]","volume":"7","author":"Chauvaud","year":"1984","journal-title":"J. Fr. Ophtalmol."},{"key":"10.1016\/j.micpath.2025.107648_bib23","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.virusres.2010.07.030","article-title":"The PRRSV replicase: exploring the multifunctionality of an intriguing set of nonstructural proteins","volume":"154","author":"Fang","year":"2010","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2025.107648_bib24","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1161\/CIRCRESAHA.124.323891","article-title":"Inflammation in HIV and its impact on atherosclerotic cardiovascular disease","volume":"134","author":"Obare","year":"2024","journal-title":"Circ. Res."},{"key":"10.1016\/j.micpath.2025.107648_bib25","article-title":"Induction of STAT1 phosphorylation at serine 727 and expression of proinflammatory cytokines by porcine reproductive and respiratory syndrome virus","volume":"8","author":"Yu","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.107648_bib26","first-page":"901","article-title":"Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae","volume":"11","author":"Thanawongnuwech","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.micpath.2025.107648_bib27","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.vetimm.2010.01.002","article-title":"Expression of inflammatory cytokines and Toll-like receptors in the brain and respiratory tract of pigs infected with porcine reproductive and respiratory syndrome virus","volume":"135","author":"Miguel","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.micpath.2025.107648_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103696","article-title":"Activation of Fc gamma receptor IIb up-regulates the production of interferon-alpha and interferon-gamma in porcine alveolar macrophages during PRRSV infection","volume":"109","author":"Zhang","year":"2020","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.micpath.2025.107648_bib29","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.vetmic.2018.10.030","article-title":"Porcine reproductive and respiratory syndrome virus induces interleukin-1\u03b2 through MyD88\/ERK\/AP-1 and NLRP3 inflammasome in microglia","volume":"227","author":"Chen","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.107648_bib30","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2021.706251","article-title":"Neferine ameliorates sepsis-induced myocardial dysfunction through anti-apoptotic and antioxidative effects by regulating the PI3K\/AKT\/mTOR signaling pathway","volume":"12","author":"Qi","year":"2021","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.micpath.2025.107648_bib31","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s00011-019-01256-6","article-title":"Neferine inhibits LPS-ATP-induced endothelial cell pyroptosis via regulation of ROS\/NLRP3\/Caspase-1 signaling pathway","volume":"68","author":"Tang","year":"2019","journal-title":"Inflamm. Res. : Off. J. Eur. Histamine Res. Society [et al]"},{"key":"10.1016\/j.micpath.2025.107648_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2023.154798","article-title":"Neferine ameliorates nonalcoholic steatohepatitis through regulating AMPK pathway","volume":"114","author":"Wang","year":"2023","journal-title":"Phytomedicine : Int. J. Phytother. Phytopharm."},{"key":"10.1016\/j.micpath.2025.107648_bib33","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.cell.2008.01.020","article-title":"Shared principles in NF-kappaB signaling","volume":"132","author":"Hayden","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.micpath.2025.107648_bib34","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/s41577-020-0288-3","article-title":"RIG-I-like receptors: their regulation and roles in RNA sensing","volume":"20","author":"Rehwinkel","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.micpath.2025.107648_bib35","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.3389\/fimmu.2019.01846","article-title":"Highly efficient generation of pigs harboring a partial deletion of the CD163 SRCR5 domain, which are fully resistant to porcine reproductive and respiratory syndrome virus 2 infection","volume":"10","author":"Guo","year":"2019","journal-title":"Front. Immunol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025003730?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025003730?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T03:07:50Z","timestamp":1762312070000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401025003730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":35,"alternative-id":["S0882401025003730"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107648","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Neferine inhibits PRRSV infection by disrupting p65 nuclear translocation","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107648","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107648"}}