{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:29:39Z","timestamp":1790314179797,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100011753","name":"National Institute of Immunology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011753","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100026280","name":"BRIC","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100026280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018842","name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018842","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.micpath.2025.107974","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T15:52:39Z","timestamp":1754495559000},"page":"107974","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["SPD_1629 is a uracil transporter and is crucial for pneumococcal fitness and pathogenesis in vivo"],"prefix":"10.1016","volume":"208","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3399-2843","authenticated-orcid":false,"given":"Ajay","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabnam","family":"Shabnam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9819-4100","authenticated-orcid":false,"given":"Sachin","family":"Kushwaha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4869-7105","authenticated-orcid":false,"given":"Devinder","family":"Sehgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2025.107974_bib2","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01422-24","article-title":"Antibiotic susceptibility of pathogens isolated in respiratory tract samples of recently hospitalized patients","volume":"13","author":"Reiner-Benaim","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micpath.2025.107974_bib3","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.GPP3-0019-2018","article-title":"Streptococcus pneumoniae capsular polysaccharide","volume":"7","author":"Paton","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micpath.2025.107974_bib4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0008-6215(00)82669-5","article-title":"The structure of capsular polysaccharide of the pneumococcus type II","volume":"40","author":"Kenne","year":"1975","journal-title":"Carbohydr. Res."},{"key":"10.1016\/j.micpath.2025.107974_bib5","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.fmrre.2005.04.006","article-title":"Nucleotide metabolism and its control in lactic acid bacteria","volume":"29","author":"Kilstrup","year":"2005","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.micpath.2025.107974_bib6","doi-asserted-by":"crossref","first-page":"321","DOI":"10.3389\/fmicb.2018.00321","article-title":"Interplay between capsule expression and uracil metabolism in Streptococcus pneumoniae D39","volume":"9","author":"Carvalho","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.107974_bib7","doi-asserted-by":"crossref","first-page":"6757","DOI":"10.1128\/IAI.72.12.6757-6763.2004","article-title":"ATP-binding cassette transporters are targets for the development of antibacterial vaccines and therapies","volume":"72","author":"Garmory","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.107974_bib8","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1126\/science.1061217","article-title":"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae","volume":"293","author":"Tettelin","year":"2001","journal-title":"Science."},{"key":"10.1016\/j.micpath.2025.107974_bib9","doi-asserted-by":"crossref","first-page":"36827","DOI":"10.1074\/jbc.M113.523340","article-title":"Functional identification of the hypoxanthine\/guanine transporters YjcD and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-12","volume":"288","author":"Papakostas","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.micpath.2025.107974_bib10","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1080\/09687860500092927","article-title":"Cloning and functional characterization of two bacterial members of the NAT\/NCS2 family in Escherichia coli","volume":"22","author":"Karatza","year":"2005","journal-title":"Mol. Membr. Biol."},{"key":"10.1016\/j.micpath.2025.107974_bib11","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/nature09885","article-title":"Structure and mechanism of the uracil transporter UraA","volume":"472","author":"Lu","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2025.107974_bib12","doi-asserted-by":"crossref","first-page":"3086","DOI":"10.1128\/IAI.00514-12","article-title":"Borrelia burgdorferi harbors a transport system essential for purine salvage and mammalian infection","volume":"80","author":"Jain","year":"2012","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.107974_bib13","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/mmi.13931","article-title":"Insight on specificity of uracil permeases of the NAT\/NCS2 family from analysis of the transporter encoded in the pyrimidine utilization operon of Escherichia coli","volume":"108","author":"Botou","year":"2018","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micpath.2025.107974_bib14","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.micpath.2018.08.059","article-title":"Functional characterization of the uracil transporter from honeybee pathogen Paenibacillus larvae","volume":"124","author":"Stoffer-Bittner","year":"2018","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2025.107974_bib15","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02434-24","article-title":"Substrate identification of putative NCS1 and NCS2 nucleobase transporters in Pseudomonas aeruginosa","volume":"15","author":"Kennelly","year":"2024","journal-title":"mBio"},{"key":"10.1016\/j.micpath.2025.107974_bib16","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1039\/b414739a","article-title":"The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands","volume":"3","author":"Patching","year":"2005","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.micpath.2025.107974_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0118154","article-title":"Conserved surface accessible nucleoside ABC transporter component SP0845 is essential for pneumococcal virulence and confers protection in vivo","volume":"10","author":"Saxena","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.107974_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2020.11.022","article-title":"Crystal structure and pathophysiological role of the pneumococcal nucleoside-binding protein PnrA","volume":"433","author":"Abdullah","year":"2021","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.micpath.2025.107974_bib19","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1101\/gr.137430.112","article-title":"A fine scale phenotype-genotype virulence map of a bacterial pathogen","volume":"22","author":"van Opijnen","year":"2012","journal-title":"Genome Res."},{"key":"10.1016\/j.micpath.2025.107974_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1011188","article-title":"RNA cis-regulators are important for Streptococcus pneumoniae in vivo success","volume":"20","author":"Warrier","year":"2024","journal-title":"PLoS Genet."},{"key":"10.1016\/j.micpath.2025.107974_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1007461","article-title":"The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence","volume":"14","author":"Warrier","year":"2018","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micpath.2025.107974_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijmm.2025.151648","article-title":"The MgaSpn global transcriptional regulator mediates the biosynthesis of capsular polysaccharides and affects virulence via the uracil synthesis pathway in Streptococcus pneumoniae","volume":"318","author":"Wang","year":"2025","journal-title":"Int J Med Microbiol"},{"key":"10.1016\/j.micpath.2025.107974_bib23","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1042\/bj20030284","article-title":"Relationship between bacterial virulence and nucleotide metabolism: a mutation in the adenylate kinase gene renders Yersinia pestis avirulent","volume":"373","author":"Munier-Lehmann","year":"2003","journal-title":"Biochem. J."},{"key":"10.1016\/j.micpath.2025.107974_bib24","doi-asserted-by":"crossref","first-page":"e37","DOI":"10.1371\/journal.ppat.0040037","article-title":"Nucleotide biosynthesis is critical for growth of bacteria in human blood","volume":"4","author":"Samant","year":"2008","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micpath.2025.107974_bib25","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1128\/JB.05757-11","article-title":"Helicobacter pylori relies primarily on the purine salvage pathway for purine nucleotide biosynthesis","volume":"194","author":"Liechti","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micpath.2025.107974_bib26","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1128\/jb.88.1.158-164.1964","article-title":"Chemically defined medium for growth Streptococcus pyogenes","volume":"88","author":"Mickelson","year":"1964","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micpath.2025.107974_bib27","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1128\/iai.27.2.444-448.1980","article-title":"Growth characteristics of group A streptococci in a new chemically defined medium","volume":"27","author":"van de Rijn","year":"1980","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.107974_bib28","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1128\/jb.91.6.2245-2250.1966","article-title":"Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium","volume":"91","author":"Willett","year":"1966","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micpath.2025.107974_bib29","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1099\/mic.0.28521-0","article-title":"To have neighbour's fare: extending the molecular toolbox for Streptococcus pneumoniae","volume":"152","author":"Kloosterman","year":"2006","journal-title":"Microbiology (Read.)"},{"key":"10.1016\/j.micpath.2025.107974_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0058492","article-title":"Environmental and nutritional factors that affect growth and metabolism of the pneumococcal serotype 2 strain D39 and its nonencapsulated derivative strain R6","volume":"8","author":"Carvalho","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.107974_bib31","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giy133","article-title":"NAT\/NCS2-hound: a webserver for the detection and evolutionary classification of prokaryotic and eukaryotic nucleobase-cation symporters of the NAT\/NCS2 family","volume":"7","author":"Chaliotis","year":"2018","journal-title":"GigaScience"},{"key":"10.1016\/j.micpath.2025.107974_bib32","doi-asserted-by":"crossref","first-page":"5570","DOI":"10.4049\/jimmunol.0803254","article-title":"Molecular dissection of antibody responses against pneumococcal surface protein A: evidence for diverse DH-less heavy chain gene usage and avidity maturation","volume":"182","author":"Rohatgi","year":"2009","journal-title":"J. Immunol."},{"key":"10.1016\/j.micpath.2025.107974_bib35","doi-asserted-by":"crossref","first-page":"7985","DOI":"10.1038\/s41598-018-25865-z","article-title":"A novel extracellular vesicle-associated endodeoxyribonuclease helps Streptococcus pneumoniae evade neutrophil extracellular traps and is required for full virulence","volume":"8","author":"Jhelum","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.107974_bib36","doi-asserted-by":"crossref","first-page":"6087","DOI":"10.1128\/jb.178.20.6087-6090.1996","article-title":"Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone","volume":"178","author":"Pozzi","year":"1996","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micpath.2025.107974_bib37","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/0003-2697(64)90164-2","article-title":"Determination of glucuronic acid by naphthoresorcinol","volume":"8","author":"Nir","year":"1964","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.micpath.2025.107974_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2020.104363","article-title":"An Erwinia amylovora uracil transporter mutant retains virulence on immature apple and pear fruit","volume":"147","author":"Bittner","year":"2020","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2025.107974_bib39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005951","article-title":"Pyruvate oxidase as a critical link between metabolism and capsule biosynthesis in Streptococcus pneumoniae","volume":"12","author":"Echlin","year":"2016","journal-title":"PLoS Pathog."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025006990?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401025006990?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:48:13Z","timestamp":1772797693000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401025006990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":36,"alternative-id":["S0882401025006990"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107974","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"SPD_1629 is a uracil transporter and is crucial for pneumococcal fitness and pathogenesis in vivo","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2025.107974","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107974"}}