{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:29:58Z","timestamp":1780406998462,"version":"3.54.1"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008656","name":"Innovations in Infectious Diseases Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008656","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.micpath.2025.108081","type":"journal-article","created":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T00:44:07Z","timestamp":1759538647000},"page":"108081","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Modeling mycoplasma infections: insights from Air-Liquid Interface cultures of primary bronchial epithelial cells"],"prefix":"10.1016","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8175-866X","authenticated-orcid":false,"given":"Fabienne","family":"Archer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Erny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2198-5871","authenticated-orcid":false,"given":"Cyrielle","family":"Nanquette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9357-1528","authenticated-orcid":false,"given":"Sandra","family":"Lac\u00f4te","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8390-8123","authenticated-orcid":false,"given":"Patrice","family":"Gaurivaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3968-4801","authenticated-orcid":false,"given":"Florence","family":"Tardy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2025.108081_bib1","series-title":"Bergey's Manual of Systematics of Archaea and Bacteria, Online","article-title":"Mycoplasma","author":"Balish","year":"2024"},{"key":"10.1016\/j.micpath.2025.108081_bib2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.cvfa.2020.02.002","article-title":"Pathogenesis and virulence of Mycoplasma bovis","volume":"36","author":"Perez-Casal","year":"2020","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.micpath.2025.108081_bib3","article-title":"Mycoplasma bovis infections-occurrence, diagnosis and control","volume":"9","author":"Dudek","year":"2020","journal-title":"Pathogens"},{"key":"10.1016\/j.micpath.2025.108081_bib4","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1186\/s12917-021-03037-y","article-title":"Identification of Mycoplasma species and related organisms from ruminants in England and Wales during 2005-2019","volume":"17","author":"Deeney","year":"2021","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.micpath.2025.108081_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2021.667175","article-title":"Addressing the antimicrobial resistance of ruminant mycoplasmas using a clinical surveillance network","volume":"8","author":"Jay","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.micpath.2025.108081_bib6","doi-asserted-by":"crossref","first-page":"589","DOI":"10.3168\/jds.2022-21929","article-title":"Pathogenic infection characteristics and risk factors for bovine respiratory disease complex based on the detection of lung pathogens in dead cattle in Northeast China","volume":"106","author":"Zhou","year":"2023","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.micpath.2025.108081_bib7","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0034-5288(18)32836-4","article-title":"Pathogenicity of some Mycoplasma and Acholeplasma species in the lungs of gnotobiotic calves","volume":"27","author":"Gourlay","year":"1979","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.micpath.2025.108081_bib8","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1186\/BF03547517","article-title":"Failure to produce pneumonia in calves by inhalation of Mycoplasma bovirhinis and Ureaplasma aerosols","volume":"22","author":"Friis","year":"1981","journal-title":"Acta Vet. Scand."},{"key":"10.1016\/j.micpath.2025.108081_bib9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.vetmic.2008.12.024","article-title":"Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response","volume":"137","author":"Angen","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s42523-022-00167-y","article-title":"Identification of bovine respiratory disease through the nasal microbiome","volume":"4","author":"Centeno-Martinez","year":"2022","journal-title":"Anim. Microbiome"},{"key":"10.1016\/j.micpath.2025.108081_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens13020114","article-title":"The role of Mycoplasma bovirhinis in the development of singular and concomitant respiratory infections in dairy calves from Southern Brazil","volume":"13","author":"Frucchi","year":"2024","journal-title":"Pathogens"},{"key":"10.1016\/j.micpath.2025.108081_bib12","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1006\/mpat.1996.0078","article-title":"Differential and strain-specific triggering of bovine alveolar macrophage effector functions by mycoplasmas","volume":"21","author":"Jungi","year":"1996","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2025.108081_bib13","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1534\/g3.118.200018","article-title":"Genome-Wide analysis of Mycoplasma bovirhinis GS01 reveals potential virulence factors and phylogenetic relationships","volume":"8","author":"Chen","year":"2018","journal-title":"G3 (Bethesda)"},{"key":"10.1016\/j.micpath.2025.108081_bib14","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms8091351","article-title":"Mycoplasmas-Host interaction: mechanisms of inflammation and association with cellular transformation","volume":"8","author":"Benedetti","year":"2020","journal-title":"Microorganisms"},{"key":"10.1016\/j.micpath.2025.108081_bib15","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/S0966-842X(98)01358-4","article-title":"Subversion and exploitation of host cells by mycoplasmas","volume":"6","author":"Rottem","year":"1998","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib16","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.cvfa.2019.08.003","article-title":"Mycoplasma bovis: interactions with the immune system and failure to generate an effective immune response","volume":"35","author":"Maunsell","year":"2019","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.micpath.2025.108081_bib17","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.vetmic.2016.02.016","article-title":"Mycoplasma bovis isolates recovered from cattle and bison (Bison bison) show differential in vitro effects on PBMC proliferation, alveolar macrophage apoptosis and invasion of epithelial and immune cells","volume":"186","author":"Suleman","year":"2016","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib18","doi-asserted-by":"crossref","first-page":"4570","DOI":"10.1128\/IAI.00707-10","article-title":"Invasion of bovine peripheral blood mononuclear cells and erythrocytes by Mycoplasma bovis","volume":"78","author":"van der Merwe","year":"2010","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.108081_bib19","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13567-015-0194-z","article-title":"Invasion and persistence of Mycoplasma bovis in embryonic calf turbinate cells","volume":"46","author":"Burki","year":"2015","journal-title":"Vet. Res."},{"key":"10.1016\/j.micpath.2025.108081_bib20","doi-asserted-by":"crossref","first-page":"329","DOI":"10.3389\/fcimb.2018.00329","article-title":"Bovine Epithelial in vitro Infection Models for Mycoplasma bovis","volume":"8","author":"Josi","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109013","article-title":"Mycoplasmas bovis P48 induces apoptosis in EBL cells via an endoplasmic reticulum stress-dependent signaling pathway","volume":"255","author":"Wu","year":"2021","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib22","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms8020164","article-title":"Mbov_0503 encodes a novel cytoadhesin that facilitates Mycoplasma bovis interaction with tight junctions","volume":"8","author":"Zhu","year":"2020","journal-title":"Microorganisms"},{"key":"10.1016\/j.micpath.2025.108081_bib23","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.3389\/fmicb.2019.02753","article-title":"Extracellular DNA: a nutritional trigger of Mycoplasma bovis cytotoxicity","volume":"10","author":"Zhu","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib24","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0378-1135(02)00303-6","article-title":"Adherence to various host cell lines of Mycoplasma bovis strains differing in pathogenic and cultural features","volume":"91","author":"Thomas","year":"2003","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2020.108956","article-title":"Invasion of Mycoplasma bovis into bovine synovial cells utilizing the clathrin-dependent endocytosis pathway","volume":"253","author":"Nishi","year":"2021","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib26","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0882-4010(03)00003-2","article-title":"Adherence of Mycoplasma bovis to bovine bronchial epithelial cells","volume":"34","author":"Thomas","year":"2003","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2025.108081_bib27","series-title":"Comparative Biology of the Normal Lung","first-page":"83","article-title":"Epithelial cells of the bronchiole","author":"Plopper","year":"2015"},{"key":"10.1016\/j.micpath.2025.108081_bib28","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1242\/dev.140103","article-title":"Lung organoids: current uses and future promise","volume":"144","author":"Barkauskas","year":"2017","journal-title":"Development"},{"key":"10.1016\/j.micpath.2025.108081_bib29","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/s41598-017-19079-y","article-title":"Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract","volume":"8","author":"Cozens","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.108081_bib30","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-33180-w","article-title":"Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface","volume":"8","author":"Cozens","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.108081_bib31","article-title":"Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus","volume":"10","author":"Lee","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2025.108081_bib32","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2021.109067","article-title":"Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria","volume":"257","author":"Strassle","year":"2021","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib33","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00470-20","article-title":"Paracellular pathway-mediated Mycoplasma hyopneumoniae migration across porcine airway epithelial barrier under air-liquid interface conditions","volume":"88","author":"Wang","year":"2020","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.108081_bib34","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.12810","article-title":"Modelling persistent Mycoplasma pneumoniae infection of human airway epithelium","volume":"20","author":"Prince","year":"2018","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib35","doi-asserted-by":"crossref","DOI":"10.1128\/iai.00129-22","article-title":"Mycoplasma pneumoniae compared to Streptococcus pneumoniae avoids induction of proinflammatory epithelial cell responses despite robustly inducing TLR2 signaling","volume":"90","author":"de Groot","year":"2022","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.108081_bib36","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1128\/AEM.03280-15","article-title":"Alterations in the quinolone resistance-determining regions and fluoroquinolone resistance in clinical isolates and laboratory-derived mutants of Mycoplasma bovis: not all genotypes May be equal","volume":"82","author":"Khalil","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib37","doi-asserted-by":"crossref","first-page":"e1623","DOI":"10.1002\/term.2589","article-title":"Bioengineered lungs generated from human iPSCs-derived epithelial cells on native extracellular matrix","volume":"12","author":"Ghaedi","year":"2018","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"10.1016\/j.micpath.2025.108081_bib38","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2021.696525","article-title":"Mycobacterial infection of precision-cut lung slices reveals type 1 interferon pathway is locally induced by Mycobacterium bovis but not M. tuberculosis in a cattle breed","volume":"8","author":"Remot","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.micpath.2025.108081_bib39","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1177\/0300985813502821","article-title":"Failure of respiratory defenses in the pathogenesis of bacterial pneumonia of cattle","volume":"51","author":"Caswell","year":"2014","journal-title":"Vet. Pathol."},{"key":"10.1016\/j.micpath.2025.108081_bib40","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/CMR.17.4.697-728.2004","article-title":"Mycoplasma pneumoniae and its role as a human pathogen","volume":"17","author":"Waites","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.micpath.2025.108081_bib41","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01423-22","article-title":"The activation of the RIG-I\/MDA5 signaling pathway upon influenza D virus infection impairs the pulmonary proinflammatory response triggered by Mycoplasma bovis superinfection","volume":"97","author":"Gaudino","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2025.108081_bib42","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens13111003","article-title":"Mycoplasma bovis invades non-phagocytic cells by clathrin-dependent endocytic pathways and escapes from phagocytic vesicles","volume":"13","author":"Li","year":"2024","journal-title":"Pathogens"},{"key":"10.1016\/j.micpath.2025.108081_bib43","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jcpa.2020.01.005","article-title":"Intracellular localization of Mycoplasma bovis in the bronchiolar epithelium of experimentally infected calves","volume":"176","author":"Nunoya","year":"2020","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.micpath.2025.108081_bib44","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.ijmm.2018.07.006","article-title":"P27 (MBOV_RS03440) is a novel fibronectin binding adhesin of Mycoplasma bovis","volume":"308","author":"Chen","year":"2018","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib45","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.3389\/fmicb.2019.02085","article-title":"Large-scale analysis of the Mycoplasma bovis genome identified non-essential, adhesion- and virulence-related genes","volume":"10","author":"Josi","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib46","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1128\/IAI.68.2.680-687.2000","article-title":"Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins","volume":"68","author":"Sachse","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2025.108081_bib47","article-title":"alpha-Enolase, an adhesion-related factor of Mycoplasma bovis","volume":"7","author":"Song","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2025.108081_bib48","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0378-1135(93)90097-Q","article-title":"Inhibition of Mycoplasma bovis cytadherence by a monoclonal antibody and various carbohydrate substances","volume":"36","author":"Sachse","year":"1993","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejps.2023.106596","article-title":"On the path to predicting immune responses in the lung: modeling the pulmonary innate immune system at the air-liquid interface (ALI)","volume":"191","author":"Graf","year":"2023","journal-title":"Eur. J. Pharmaceut. Sci."},{"key":"10.1016\/j.micpath.2025.108081_bib50","doi-asserted-by":"crossref","first-page":"414","DOI":"10.3389\/fmicb.2016.00414","article-title":"Inflammation-inducing factors of Mycoplasma pneumoniae","volume":"7","author":"Shimizu","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2025.108081_bib51","doi-asserted-by":"crossref","first-page":"5239","DOI":"10.1038\/s44319-024-00296-2","article-title":"The Dectin-1 and Dectin-2 clusters: C-Type lectin receptors with fundamental roles in immunity","volume":"25","author":"Malamud","year":"2024","journal-title":"EMBO Rep."},{"key":"10.1016\/j.micpath.2025.108081_bib52","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006485","article-title":"Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum","volume":"13","author":"Chang","year":"2017","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micpath.2025.108081_bib53","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.micinf.2014.08.005","article-title":"Transcriptional regulation of CCL20 expression","volume":"16","author":"Zhao","year":"2014","journal-title":"Microb. Infect."},{"key":"10.1016\/j.micpath.2025.108081_bib54","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/s41420-023-01524-9","article-title":"The role of CXCL family members in different diseases","volume":"9","author":"Zhou","year":"2023","journal-title":"Cell Death Discov."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S088240102500806X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S088240102500806X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T09:05:52Z","timestamp":1763370352000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S088240102500806X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":54,"alternative-id":["S088240102500806X"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2025.108081","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Modeling mycoplasma infections: insights from Air-Liquid Interface cultures of primary bronchial epithelial cells","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2025.108081","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"108081"}}