{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:49:20Z","timestamp":1792111760003,"version":"4.4.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013338","name":"Major Technology Innovation of Hubei Province","doi-asserted-by":"publisher","award":["2025BBB004"],"award-info":[{"award-number":["2025BBB004"]}],"id":[{"id":"10.13039\/501100013338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.micpath.2026.108675","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T06:52:36Z","timestamp":1783061556000},"page":"108675","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Biological control evaluation of bacteriophage freeze-dried powder against Aeromonas hydrophila infection, based on whey protein\/trehalose"],"prefix":"10.1016","volume":"218","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congcong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4789-0605","authenticated-orcid":false,"given":"Min","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2026.108675_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms8010129","article-title":"An update on the genus aeromonas: taxonomy, epidemiology, and pathogenicity","author":"Fern\u00e1ndez-Bravo","year":"2020","journal-title":"Microorganisms"},{"key":"10.1016\/j.micpath.2026.108675_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2011.09.016","article-title":"Over-expression, purification and immune responses to Aeromonas hydrophila AL09-73 flagellar proteins","author":"Yeh","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.micpath.2026.108675_bib3","doi-asserted-by":"crossref","DOI":"10.1111\/j.1365-2672.2004.02336.x","article-title":"Maintenance of pathogenicity during entry into and resuscitation from viable but nonculturable state in Aeromonas hydrophila exposed to natural seawater at low temperature","author":"Maalej","year":"2004","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib4","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-015-0959-z","article-title":"Antibiotic use for Vibrio infections: important insights from surveillance data","author":"Wong","year":"2015","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.micpath.2026.108675_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.69.11.6628-6633.2003","article-title":"Sampling natural viral communities from soil for culture-independent analyses","author":"Williamson","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib6","series-title":"Characterization and Genome Analyses of the Novel Phages P2 and vB_AhydM-H1 Targeting","author":"Nagar","year":"2024"},{"key":"10.1016\/j.micpath.2026.108675_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2017.01.003","article-title":"Bacteriophage therapy for the control of Vibrio harveyi in greenlip abalone (Haliotis laevigata)","author":"Wang","year":"2017","journal-title":"Aquaculture"},{"key":"10.1016\/j.micpath.2026.108675_bib8","article-title":"Isolation and characterization of phage AHP-1 and its combined effect with chloramphenicol to control Aeromonas hydrophila","author":"Chandrarathna","year":"2019","journal-title":"Braz. J. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib9","doi-asserted-by":"crossref","DOI":"10.3390\/ph14040359","article-title":"Formulations for bacteriophage therapy and the potential uses of immobilization","author":"Rosner","year":"2021","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.micpath.2026.108675_bib10","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01979-20","article-title":"Encapsulation and delivery of therapeutic phages","author":"Loh","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.powtec.2022.117516","article-title":"Effect of freeze drying, spray drying and electrospraying on the morphological, thermal, and structural properties of powders containing phage Felix O1 and activity of phage Felix O1 during storage","author":"Ergin","year":"2022","journal-title":"Powder Technol."},{"key":"10.1016\/j.micpath.2026.108675_bib12","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/6943078","article-title":"Innovative and emerging drying technologies for enhancing food quality","author":"Xiao","year":"2018","journal-title":"J. Food Qual."},{"key":"10.1016\/j.micpath.2026.108675_bib13","article-title":"Glycosylated peptides isolated from cheese whey have antifreezing activity","author":"Fomich","year":"2024","journal-title":"Food Chem."},{"issue":"10","key":"10.1016\/j.micpath.2026.108675_bib14","article-title":"Rational selection of sugars for biotherapeutic stabilization:a practitioner's perspective","volume":"16","author":"Gutka","year":"2018","journal-title":"Bioprocess Int."},{"key":"10.1016\/j.micpath.2026.108675_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2017.01.060","article-title":"Effects of storage conditions on the stability of spray dried, inhalable bacteriophage powders","author":"Leung","year":"2017","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.micpath.2026.108675_bib16","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.foodcont.2018.07.043","article-title":"Occurrence of four pathogenic Vibrios in Chinese freshwater fish farms in 2016","volume":"95","author":"Yan","year":"2019","journal-title":"Food Control"},{"issue":"18","key":"10.1016\/j.micpath.2026.108675_bib17","doi-asserted-by":"crossref","first-page":"7901","DOI":"10.1007\/s00253-018-9219-7","article-title":"Characterization of a novel phage infecting the pathogenic multidrug-resistant Bacillus cereus and functional analysis of its endolysin","volume":"102","author":"Peng","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"1","key":"10.1016\/j.micpath.2026.108675_bib18","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98457-z","article-title":"Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa","volume":"11","author":"Sharma","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2026.108675_bib19","doi-asserted-by":"crossref","DOI":"10.33314\/jnhrc.v19i04.3837","article-title":"Isolation and characterization of lytic bacteriophage against multi-drug resistant Pseudomonas aeruginosa","author":"Maharjan","year":"2022","journal-title":"J. Nepal Health Res. Council"},{"issue":"3","key":"10.1016\/j.micpath.2026.108675_bib20","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.cryobiol.2013.03.007","article-title":"Effect of buffer systems and disaccharides concentration on Podoviridae coliphage stability during freeze drying and storage","volume":"66","author":"Dini","year":"2013","journal-title":"Cryobiology"},{"key":"10.1016\/j.micpath.2026.108675_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2016.01219","article-title":"Establishment of infection models in zebrafish larvae (Danio rerio) to Study the pathogenesis of Aeromonas hydrophila","volume":"7","author":"Saraceni","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib22","article-title":"Characterization of bacteriophage pAh-1 and its protective effects on experimental infection of Aeromonas hydrophila in Zebrafish (Danio rerio)","author":"Easwaran","year":"2016","journal-title":"J. Fish. Dis."},{"issue":"3","key":"10.1016\/j.micpath.2026.108675_bib23","doi-asserted-by":"crossref","DOI":"10.3390\/v13030477","article-title":"Molecular characterization of Ahp2, a lytic bacteriophage of Aeromonas hydrophila","volume":"13","author":"Wang","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2026.108675_bib24","article-title":"Biologic and genomic characterization of a novel virulent Aeromonas hydrophila phage phiA051, with high homology to prophages","volume":"11","author":"Wang","year":"2024","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.micpath.2026.108675_bib25","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2025.1679093","article-title":"Isolation and genomic analysis of phage BUCT551 against drug-resistant Aeromonas hydrophila","volume":"12","author":"Han","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.micpath.2026.108675_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2023.199193","article-title":"The characterization and genome analysis of a novel phage phiA034 targeting multiple species of Aeromonas","author":"Ye","year":"2023","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2026.108675_bib27","article-title":"Evaluation of bacteriophage therapy of Aeromonas hydrophila infection in a freshwater fish, Pangasius buchanani","volume":"2","author":"Kumari","year":"2023","journal-title":"Front. Aquacult."},{"key":"10.1016\/j.micpath.2026.108675_bib28","article-title":"Comprehensive investigation of a novel and safe lytic phage vB_AroA_KFSA9 as a commercial candidate for biocontrol of Aeromonas hydrophila on fresh produce","author":"Choi","year":"2025","journal-title":"Curr. Res. Food Sci."},{"key":"10.1016\/j.micpath.2026.108675_bib29","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.898961","article-title":"Lytic bacteriophage PZL-Ah152 as biocontrol measures against lethal Aeromonas hydrophila without distorting Gut Microbiota","volume":"13","author":"Feng","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib30","article-title":"Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila","author":"Liu","year":"2020","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.virol.2023.109887","article-title":"Whole genome sequence analysis of Aeromonas-infecting bacteriophage AHPMCC7, a new species of genus ahphunavirus and its application in litopenaeus vannamei culture","author":"Ghosh","year":"2023","journal-title":"Virology"},{"key":"10.1016\/j.micpath.2026.108675_bib32","article-title":"Characterization of a novel broad-spectrum endolysin PlyD4 encoded by a highly conserved prophage found in Aeromonas hydrophila ST251 strains","author":"Wang","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.micpath.2026.108675_bib33","doi-asserted-by":"crossref","DOI":"10.1007\/s13205-024-04101-8","article-title":"Phage therapy: a novel approach against multidrug-resistant pathogens","author":"Kapoor","year":"2024","journal-title":"3 Biotech"},{"key":"10.1016\/j.micpath.2026.108675_bib34","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-54068-3","article-title":"The use of biological seed coatings based on bacteriophages and polymers against Clavibacter michiganensis subsp. nebraskensis in maize seeds","author":"Kimmelshue","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2026.108675_bib35","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2019.00488","article-title":"Atmospheric spray freeze drying of sugar solution with phage D29","author":"Ly","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2026.108675_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.ifset.2021.102829","article-title":"Progressive freeze concentration of whey protein\u2013sucrose\u2013salt mixtures","author":"Vuist","year":"2021","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"10.1016\/j.micpath.2026.108675_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2010.01.034","article-title":"Stabilization of bacteriophage during freeze drying","author":"Puapermpoonsiri","year":"2010","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.micpath.2026.108675_bib38","doi-asserted-by":"crossref","DOI":"10.1023\/A:1013054431517","article-title":"Effect of moisture on the stability of a lyophilized humanized monoclonal antibody formulation","author":"Breen","year":"2001","journal-title":"Pharm. Res."},{"key":"10.1016\/j.micpath.2026.108675_bib39","doi-asserted-by":"crossref","DOI":"10.3390\/foods14244197","article-title":"Modulating protein glycation in skim milk powder via low humidity dry heating to improve its heat-stabilizing properties","author":"Zhao","year":"2025","journal-title":"Foods"},{"key":"10.1016\/j.micpath.2026.108675_bib40","doi-asserted-by":"crossref","DOI":"10.1208\/s12249-020-01673-5","article-title":"The stabilizing excipients in dry state therapeutic phage formulations","author":"Zhang","year":"2020","journal-title":"AAPS PharmSciTech"},{"key":"10.1016\/j.micpath.2026.108675_bib41","doi-asserted-by":"crossref","DOI":"10.1038\/nature25178","article-title":"Dietary trehalose enhances virulence of epidemic Clostridium difficile","author":"Collins","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2026.108675_bib42","doi-asserted-by":"crossref","DOI":"10.1021\/acs.jctc.9b01071","article-title":"Properties of aqueous trehalose mixtures: glass transition and hydrogen bonding","author":"Olgenblum","year":"2020","journal-title":"J. Chem. Theor. Comput."},{"key":"10.1016\/j.micpath.2026.108675_bib43","article-title":"Bacteriophage encapsulation using spray drying for phage therapy","author":"Malik","year":"2020","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.micpath.2026.108675_bib44","article-title":"Moisture sorption isotherm and thermodynamic properties of centella asiatica L. (CAL) powder","author":"Azhar","year":"2020","journal-title":"Chem. Eng. Commun."},{"key":"10.1016\/j.micpath.2026.108675_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.fpsl.2023.101095","article-title":"Incorporation of A511 bacteriophage in a whey protein isolate-based edible coating for the control of Listeria monocytogenes in cheese","author":"Garc\u00eda-Anaya","year":"2023","journal-title":"Food Packag. Shelf Life"},{"key":"10.1016\/j.micpath.2026.108675_bib46","doi-asserted-by":"crossref","DOI":"10.1007\/s11694-024-02528-4","article-title":"Microencapsulation of Lacticaseibacillus rhamnosus GG ATCC 53103 by freeze-drying: evaluation of storage stability and survival in simulated infant gastrointestinal digestion","author":"Izadi","year":"2024","journal-title":"J. Food Meas. Char."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401026004018?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401026004018?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T11:39:07Z","timestamp":1786016347000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401026004018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":46,"alternative-id":["S0882401026004018"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2026.108675","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biological control evaluation of bacteriophage freeze-dried powder against Aeromonas hydrophila infection, based on whey protein\/trehalose","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2026.108675","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"108675"}}