{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T16:03:21Z","timestamp":1789833801260,"version":"4.0.1"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100009392","name":"Prince Sattam bin Abdulaziz University","doi-asserted-by":"publisher","award":["PSAU\/2024\/03\/31454"],"award-info":[{"award-number":["PSAU\/2024\/03\/31454"]}],"id":[{"id":"10.13039\/100009392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.micpath.2026.108782","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T23:16:35Z","timestamp":1787181395000},"page":"108782","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Integrated in vitro and in silico evaluation of the antimicrobial and bioactive properties of natural honey and its enhanced derivatives against multidrug-resistant pathogens"],"prefix":"10.1016","volume":"220","author":[{"given":"Ghadah S.","family":"Abusalim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4045-0810","authenticated-orcid":false,"given":"Mohammed","family":"Alissa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2026.108782_bib1","doi-asserted-by":"crossref","first-page":"616","DOI":"10.3390\/molecules27030616","article-title":"Multidrug resistance (MDR): a widespread phenomenon in pharmacological therapies","volume":"27","author":"Catalano","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.micpath.2026.108782_bib2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3949\/ccjm.80a.12182","article-title":"Carbapenem-resistant enterobacteriaceae: a menace to our most vulnerable patients","volume":"80","author":"Perez","year":"2013","journal-title":"Cleve. Clin. J. Med."},{"key":"10.1016\/j.micpath.2026.108782_bib3","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.micpath.2026.108782_bib4","first-page":"709","article-title":"Multidrug resistant bacteria in the community: an update","volume":"34","author":"van Duin","year":"2020","journal-title":"Infect. Dis. Clin."},{"key":"10.1016\/j.micpath.2026.108782_bib5","first-page":"369","article-title":"Antioxidant and antibacterial activity of soil Streptomyces isolates collected from Mogadishu against beta-lactamase-resistant bacteria","volume":"6","author":"Ali","year":"2025","journal-title":"Microb. Infect. Dis."},{"key":"10.1016\/j.micpath.2026.108782_bib6","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.3390\/molecules25051252","article-title":"Antibacterial action mechanisms of honey: physiological effects of avocado, chestnut, and polyfloral honey upon Staphylococcus aureus and Escherichia coli","volume":"25","author":"Combarros-Fuertes","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.micpath.2026.108782_bib7","doi-asserted-by":"crossref","first-page":"551","DOI":"10.3390\/antibiotics10050551","article-title":"Honey as an ecological reservoir of antibacterial compounds produced by antagonistic microbial interactions in plant nectars, honey and honey Bee","volume":"10","author":"Brudzynski","year":"2021","journal-title":"Antibiotics"},{"key":"10.1016\/j.micpath.2026.108782_bib8","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.tifs.2021.11.012","article-title":"Honey antibacterial activity: a neglected aspect of honey quality assurance as functional food","volume":"118","author":"Majtan","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"10.1016\/j.micpath.2026.108782_bib9","doi-asserted-by":"crossref","first-page":"3720","DOI":"10.1016\/j.sjbs.2020.08.018","article-title":"Analysis of sugar composition and pesticides using HPLC and GC\u2013MS techniques in honey samples collected from Saudi Arabian markets","volume":"27","author":"Alghamdi","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"10.1016\/j.micpath.2026.108782_bib10","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1016\/j.sjbs.2020.10.017","article-title":"The antibacterial activities of honey","volume":"28","author":"Almasaudi","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"10.1016\/j.micpath.2026.108782_bib11","doi-asserted-by":"crossref","first-page":"5155","DOI":"10.3390\/polym14235155","article-title":"Antibacterial properties of honey nanocomposite fibrous meshes","volume":"14","author":"Matharu","year":"2022","journal-title":"Polymers"},{"key":"10.1016\/j.micpath.2026.108782_bib12","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3390\/vetsci7040181","article-title":"Antibacterial activity of honey samples from Ukraine","volume":"7","author":"Cilia","year":"2020","journal-title":"Vet. Sci."},{"issue":"1","key":"10.1016\/j.micpath.2026.108782_bib13","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-54576-2","article-title":"Honey can inhibit and eliminate biofilms produced by Pseudomonas aeruginosa","volume":"9","author":"Lu","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2026.108782_bib14","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1080\/07373937.2022.2068028","article-title":"Effects of different drying techniques on the quality and bioactive compounds of plant-based products: a critical review on current trends","volume":"40","author":"Belwal","year":"2022","journal-title":"Dry. Technol."},{"key":"10.1016\/j.micpath.2026.108782_bib15","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.3390\/nano12152560","article-title":"Recent advances in honey-based nanoparticles for wound dressing: a review","volume":"12","author":"Bahari","year":"2022","journal-title":"Nanomaterials"},{"key":"10.1016\/j.micpath.2026.108782_bib16","doi-asserted-by":"crossref","first-page":"829","DOI":"10.3390\/bioengineering11080829","article-title":"Green innovation and synthesis of honeybee products-mediated nanoparticles: potential approaches and wide applications","volume":"11","author":"Khalifa","year":"2024","journal-title":"Bioengineering"},{"key":"10.1016\/j.micpath.2026.108782_bib17","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1080\/17460913.2025.2498233","article-title":"Selected honey as a multifaceted antimicrobial agent: review of compounds, mechanisms, and research challenges","volume":"20","author":"Machado","year":"2025","journal-title":"Future Microbiol."},{"key":"10.1016\/j.micpath.2026.108782_bib18","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/immuno5020019","article-title":"Immunomodulatory and anti-inflammatory properties of honey and bee products","volume":"5","author":"Saad","year":"2025","journal-title":"Immuno"},{"key":"10.1016\/j.micpath.2026.108782_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00218839.2020.1738135","article-title":"Standard methods for Apis mellifera honey research","volume":"59","author":"Bicudo de Almeida-Muradian","year":"2020","journal-title":"J. Apicult. Res."},{"key":"10.1016\/j.micpath.2026.108782_bib20","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/7383018","article-title":"Microbiological quality, physicochemical properties, and antioxidant capacity of honey samples commercialized in the Moroccan Errachidia region","volume":"2020","author":"El Menyiy","year":"2020","journal-title":"J. Food Qual."},{"key":"10.1016\/j.micpath.2026.108782_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.lwt.2021.111932","article-title":"Determination of total flavonoid content by aluminum chloride assay: a critical evaluation","volume":"150","author":"Shraim","year":"2021","journal-title":"LWT"},{"key":"10.1016\/j.micpath.2026.108782_bib22","doi-asserted-by":"crossref","first-page":"306","DOI":"10.3390\/foods9030306","article-title":"Antioxidant activity, total phenolic content, individual phenolics and physicochemical parameters suitability for Romanian honey authentication","volume":"9","author":"Pauliuc","year":"2020","journal-title":"Foods"},{"key":"10.1016\/j.micpath.2026.108782_bib23","first-page":"1114","article-title":"Antioxidant, antimicrobial activities and GCMS analysis of fruit extract of Solanum nigrum L","volume":"9","author":"Sivaraj","year":"2020","journal-title":"J. Pharmacogn. Phytochem."},{"key":"10.1016\/j.micpath.2026.108782_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2021.111934","article-title":"Antimicrobial activity of graphite oxide doped with silver against Bacillus subtilis, Candida albicans, Escherichia coli, and Staphylococcus aureus by agar well diffusion test: synthesis and characterization","volume":"123","author":"Chavez-Esquivel","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"10.1016\/j.micpath.2026.108782_bib29","doi-asserted-by":"crossref","first-page":"65","DOI":"10.3390\/antibiotics9020065","article-title":"Strategies to combat multidrug-resistant and persistent infectious diseases","volume":"9","author":"Pacios","year":"2020","journal-title":"Antibiotics"},{"issue":"1","key":"10.1016\/j.micpath.2026.108782_bib30","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s12906-023-03841-z","article-title":"Antimicrobial analysis of honey against Staphylococcus aureus isolates from wound, ADMET properties of its bioactive compounds and in-silico evaluation against dihydropteroate synthase","volume":"23","author":"Edet","year":"2023","journal-title":"BMC Complement. Med. Ther."},{"key":"10.1016\/j.micpath.2026.108782_bib31","doi-asserted-by":"crossref","first-page":"500","DOI":"10.3390\/insects11080500","article-title":"Stingless bee honey: evaluating its antibacterial activity and bacterial diversity","volume":"11","author":"Rosli","year":"2020","journal-title":"Insects"},{"key":"10.1016\/j.micpath.2026.108782_bib32","doi-asserted-by":"crossref","first-page":"272.e1","DOI":"10.1016\/j.scispo.2020.10.005","article-title":"The health benefits of honey as an energy source with antioxidant, antibacterial and antiseptic effects","volume":"36","author":"Ilia","year":"2021","journal-title":"Sci. Sports"},{"key":"10.1016\/j.micpath.2026.108782_bib33","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1080\/19476337.2019.1701559","article-title":"Bioactive compounds and antibacterial properties of monofloral Ulmo honey","volume":"18","author":"Vel\u00e1squez","year":"2020","journal-title":"CyTA J. Food"},{"key":"10.1016\/j.micpath.2026.108782_bib34","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.3390\/foods9091263","article-title":"Demanding new honey qualitative standard based on antibacterial activity","volume":"9","author":"Bucekova","year":"2020","journal-title":"Foods"},{"issue":"1","key":"10.1016\/j.micpath.2026.108782_bib43","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s12906-025-04778-1","article-title":"Exploring the antibacterial and anti-biofilm activity of two Iranian medical-grade kinds of honey on multidrug-resistant Pseudomonas aeruginosa","volume":"25","author":"Alshaybawee","year":"2025","journal-title":"BMC Complement. Med. Ther."},{"key":"10.1016\/j.micpath.2026.108782_bib35","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/873081","article-title":"Response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane","volume":"2014","author":"Mur\u00ednov\u00e1","year":"2014","journal-title":"Int. J. Microbiol."},{"key":"10.1016\/j.micpath.2026.108782_bib36","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3390\/antibiotics14030255","article-title":"Honey as a natural antimicrobial","volume":"14","author":"Ogwu","year":"2025","journal-title":"Antibiotics"},{"key":"10.1016\/j.micpath.2026.108782_bib37","doi-asserted-by":"crossref","first-page":"314","DOI":"10.3390\/antibiotics9060314","article-title":"Activity of specialized biomolecules against gram-positive and gram-negative bacteria","volume":"9","author":"Tavares","year":"2020","journal-title":"Antibiotics"},{"key":"10.1016\/j.micpath.2026.108782_bib38","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.3390\/antibiotics11091182","article-title":"Antibacterial mechanism of action of two types of honey against Escherichia coli through interfering with bacterial membrane permeability, inhibiting proteins, and inducing bacterial DNA damage","volume":"11","author":"Al-Sayaghi","year":"2022","journal-title":"Antibiotics"},{"key":"10.1016\/j.micpath.2026.108782_bib39","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.2147\/IDR.S493635","article-title":"Capsular polysaccharide as a potential target in hypervirulent and drug-resistant Klebsiella pneumoniae treatment","volume":"18","author":"Ke","year":"2025","journal-title":"Infect. Drug Resist."},{"key":"10.1016\/j.micpath.2026.108782_bib40","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3390\/biology14020165","article-title":"Biofilm resilience: molecular mechanisms driving antibiotic resistance in clinical contexts","volume":"14","author":"Almatroudi","year":"2025","journal-title":"Biology"},{"key":"10.1016\/j.micpath.2026.108782_bib41","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2024.1350391","article-title":"Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community","volume":"15","author":"Shariati","year":"2024","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.micpath.2026.108782_bib42","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3390\/biology13020109","article-title":"Bacterial biofilm in chronic wounds and possible therapeutic approaches","volume":"13","author":"Cavallo","year":"2024","journal-title":"Biology"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401026005085?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401026005085?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T15:22:21Z","timestamp":1789831341000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401026005085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":39,"alternative-id":["S0882401026005085"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2026.108782","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated in vitro and in silico evaluation of the antimicrobial and bioactive properties of natural honey and its enhanced derivatives against multidrug-resistant pathogens","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2026.108782","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"108782"}}