{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T06:10:45Z","timestamp":1784700645169,"version":"3.55.0"},"reference-count":69,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbiological Research"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1016\/j.micres.2017.03.007","type":"journal-article","created":{"date-parts":[[2017,3,19]],"date-time":"2017-03-19T09:32:52Z","timestamp":1489915972000},"page":"40-48","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Functional characterisation of Burkholderia pseudomallei biotin protein ligase: A toolkit for anti-melioidosis drug development"],"prefix":"10.1016","volume":"199","author":[{"given":"Thomas E.H.","family":"Bond","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alanna E.","family":"Sorenson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick M.","family":"Schaeffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.micres.2017.03.007_bib0005","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.chembiol.2003.09.002","article-title":"The process of structure-based drug design","volume":"10","author":"Anderson","year":"2003","journal-title":"Chem. Biol."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0010","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1039\/C5AY03064A","article-title":"Green fluorescent protein-based assays for high-throughput functional characterization and ligand-binding studies of biotin protein ligase","volume":"8","author":"Askin","year":"2016","journal-title":"Anal. Methods-UK"},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0015","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.jmb.2005.08.032","article-title":"Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation","volume":"353","author":"Bagautdinov","year":"2005","journal-title":"J. Mol. Biol."},{"issue":"4","key":"10.1016\/j.micres.2017.03.007_bib0020","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1110\/ps.8.4.921","article-title":"A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation","volume":"8","author":"Beckett","year":"1999","journal-title":"Protein Sci."},{"key":"10.1016\/j.micres.2017.03.007_bib0025","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev.genet.41.042007.170450","article-title":"Biotin sensing: universal influence of biotin status on transcription","volume":"41","author":"Beckett","year":"2007","journal-title":"Annu. Rev. Genet."},{"issue":"4","key":"10.1016\/j.micres.2017.03.007_bib0030","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/jac\/dkl020","article-title":"The role of horizontal gene transfer in the spread of trimethoprim\u2013sulfamethoxazole resistance among uropathogenic Escherichia coli in Europe and Canada","volume":"57","author":"Blahna","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"issue":"9","key":"10.1016\/j.micres.2017.03.007_bib0035","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.1128\/jb.177.9.2572-2575.1995","article-title":"Characterization of the Bacillus-Subtilis Bira gene encoding a repressor of the biotin operon","volume":"177","author":"Bower","year":"1995","journal-title":"J. Bacteriol."},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0040","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1146\/annurev.micro.55.1.305","article-title":"Bacterial fatty acid biosynthesis: targets for antibacterial drug discovery","volume":"55","author":"Campbell","year":"2001","journal-title":"Ann. Rev. Microbiol."},{"issue":"12","key":"10.1016\/j.micres.2017.03.007_bib0045","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1110\/ps.ps.22401","article-title":"The C-terminal domain of biotin protein ligase from E. coli is required for catalytic activity","volume":"10","author":"Chapman-Smith","year":"2001","journal-title":"Protein Sci."},{"issue":"6","key":"10.1016\/j.micres.2017.03.007_bib0050","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1046\/j.1432-1033.2003.03493.x","article-title":"Biotinylation in the hyperthermophile Aquifex aeolicus \u2013 isolation of a cross-linked BPL: BCCP complex","volume":"270","author":"Clarke","year":"2003","journal-title":"Eur. J. Biochem."},{"issue":"6685","key":"10.1016\/j.micres.2017.03.007_bib0055","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/31159","article-title":"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence","volume":"393","author":"Cole","year":"1998","journal-title":"Nature"},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0060","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.diagmicrobio.2012.10.011","article-title":"ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis","volume":"75","author":"Cooper","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0065","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/S0035-9203(00)90333-X","article-title":"Melioidosis: acute and chronic disease, relapse and re-activation","volume":"94","author":"Currie","year":"2000","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"6","key":"10.1016\/j.micres.2017.03.007_bib0070","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1021\/ml300106p","article-title":"Biotin analogues with antibacterial activity are potent inhibitors of biotin protein ligase","volume":"3","author":"da Costa","year":"2012","journal-title":"ACS Med. Chem. Lett."},{"issue":"21","key":"10.1016\/j.micres.2017.03.007_bib0075","doi-asserted-by":"crossref","first-page":"17823","DOI":"10.1074\/jbc.M112.356576","article-title":"Selective inhibition of biotin protein ligase from Staphylococcus aureus","volume":"287","author":"da Costa","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.micres.2017.03.007_bib0080","article-title":"Humanised biotin protein ligase provides clues about inhibitor selectivity","volume":"282","author":"da Costa","year":"2015","journal-title":"FEBS J."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0085","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0001-706X(99)00059-5","article-title":"Melioidosis as an emerging global problem","volume":"74","author":"Dance","year":"2000","journal-title":"Acta Trop."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0090","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1006\/plas.1999.1421","article-title":"Genetic exchange between bacteria in the environment","volume":"42","author":"Davison","year":"1999","journal-title":"Plasmid"},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0095","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1517\/13543780903505092","article-title":"Newer antibacterial drugs for a new century","volume":"19","author":"Devasahayam","year":"2010","journal-title":"Expert Opin. Invest. Drug"},{"issue":"11","key":"10.1016\/j.micres.2017.03.007_bib0100","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/j.chembiol.2011.08.013","article-title":"Bisubstrate adenylation inhibitors of biotin protein ligase from Mycobacterium tuberculosis","volume":"18","author":"Duckworth","year":"2011","journal-title":"Chem. Biol."},{"issue":"8","key":"10.1016\/j.micres.2017.03.007_bib0105","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/j.jmb.2015.02.021","article-title":"Allosteric coupling via distant disorder-to-order transitions","volume":"427","author":"Eginton","year":"2015","journal-title":"J. Mol. Biol."},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0110","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1586\/eri.10.4","article-title":"Present and future therapeutic strategies for melioidosis and glanders","volume":"8","author":"Estes","year":"2010","journal-title":"Expert Rev. Anti Infect. Ther."},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0115","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00591-15","article-title":"The atypical occurrence of two biotin protein ligases in Francisella novicida is due to distinct roles in virulence and biotin metabolism","volume":"6","author":"Feng","year":"2015","journal-title":"MBio"},{"issue":"25","key":"10.1016\/j.micres.2017.03.007_bib0120","doi-asserted-by":"crossref","first-page":"26066","DOI":"10.1074\/jbc.M402989200","article-title":"Identification and characterization of the first class of potent bacterial acetyl-CoA carboxylase inhibitors with antibacterial activity","volume":"279","author":"Freiberg","year":"2004","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0125","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1128\/JB.188.2.477-486.2006","article-title":"Biochemical and structural characterization of an essential acyl coenzyme A carboxylase from Mycobacterium tuberculosis","volume":"188","author":"Gago","year":"2006","journal-title":"J. Bacteriol."},{"issue":"23","key":"10.1016\/j.micres.2017.03.007_bib0130","doi-asserted-by":"crossref","first-page":"16484","DOI":"10.1074\/jbc.M113.468892","article-title":"Crystal structure of mycobacterium tuberculosis polyketide synthase 11 (PKS11) reveals intermediates in the synthesis of methyl-branched alkylpyrones","volume":"288","author":"Gokulan","year":"2013","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0135","doi-asserted-by":"crossref","first-page":"e9222","DOI":"10.1371\/journal.pone.0009222","article-title":"Structural ordering of disordered ligand-binding loops of biotin protein ligase into active conformations as a consequence of dehydration","volume":"5","author":"Gupta","year":"2010","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0140","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1146\/annurev.bi.59.070190.004441","article-title":"DNA recognition by proteins with the helix-turn-helix motif","volume":"59","author":"Harrison","year":"1990","journal-title":"Annu. Rev. Biochem."},{"issue":"5","key":"10.1016\/j.micres.2017.03.007_bib0145","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0096757","article-title":"Successful conversion of the Bacillus subtilis BirA group II biotin protein ligase into a group I ligase","volume":"9","author":"Henke","year":"2014","journal-title":"PLoS One"},{"issue":"39","key":"10.1016\/j.micres.2017.03.007_bib0150","doi-asserted-by":"crossref","first-page":"14240","DOI":"10.1073\/pnas.0403302101","article-title":"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei","volume":"101","author":"Holden","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"39","key":"10.1016\/j.micres.2017.03.007_bib0155","doi-asserted-by":"crossref","first-page":"14240","DOI":"10.1073\/pnas.0403302101","article-title":"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei","volume":"101","author":"Holden","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"5","key":"10.1016\/j.micres.2017.03.007_bib0160","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.cell.2007.06.049","article-title":"A common mechanism of cellular death induced by bactericidal antibiotics","volume":"130","author":"Kohanski","year":"2007","journal-title":"Cell"},{"issue":"8","key":"10.1016\/j.micres.2017.03.007_bib0165","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1110\/ps.9.8.1530","article-title":"Function of a conserved sequence motif in biotin holoenzyme synthetases","volume":"9","author":"Kwon","year":"2000","journal-title":"Protein Sci."},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0170","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1110\/ps.33502","article-title":"Binding specificity and the ligand dissociation process in the E. coli biotin holoenzyme synthetase","volume":"11","author":"Kwon","year":"2002","journal-title":"Protein Sci."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0175","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/S0021-9258(18)48362-7","article-title":"The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase","volume":"267","author":"Li","year":"1992","journal-title":"J. Biol. Chem."},{"issue":"7","key":"10.1016\/j.micres.2017.03.007_bib0180","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1002\/pro.2475","article-title":"Active site conformational changes upon reaction intermediate biotinyl-5\u2032-AMP binding in biotin protein ligase from Mycobacterium tuberculosis","volume":"23","author":"Ma","year":"2014","journal-title":"Protein Sci."},{"issue":"12","key":"10.1016\/j.micres.2017.03.007_bib0185","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.1128\/JCM.43.12.6032-6034.2005","article-title":"Recurrent melioidosis in patients in northeast Thailand is frequently due to reinfection rather than relapse","volume":"43","author":"Maharjan","year":"2005","journal-title":"J. Clin. Microbiol."},{"issue":"13","key":"10.1016\/j.micres.2017.03.007_bib0190","doi-asserted-by":"crossref","first-page":"5258","DOI":"10.1021\/bi048135v","article-title":"Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor","volume":"44","author":"Matulis","year":"2005","journal-title":"Biochemistry"},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0195","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00109-011-0811-x","article-title":"A novel molecular mechanism to explain biotin-unresponsive holocarboxylase synthetase deficiency","volume":"90","author":"Mayende","year":"2012","journal-title":"J. Mol. Med. (Berl.)"},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0200","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1128\/AAC.43.3.465","article-title":"Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei","volume":"43","author":"Moore","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"issue":"12","key":"10.1016\/j.micres.2017.03.007_bib0205","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1039\/c3mb70426b","article-title":"Dissecting the salt dependence of the Tus\u2013Ter protein\u2013DNA complexes by high-throughput differential scanning fluorimetry of a GFP-tagged Tus","volume":"9","author":"Moreau","year":"2013","journal-title":"Mol. Biosyst."},{"issue":"7","key":"10.1016\/j.micres.2017.03.007_bib0210","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1039\/c002001j","article-title":"Quantitative determination of protein stability and ligand binding using a green fluorescent protein reporter system","volume":"6","author":"Moreau","year":"2010","journal-title":"Mol. Biosyst."},{"issue":"31","key":"10.1016\/j.micres.2017.03.007_bib0215","doi-asserted-by":"crossref","first-page":"11892","DOI":"10.1039\/c2ra22368f","article-title":"Rapid determination of protein stability and ligand binding by differential scanning fluorimetry of GFP-tagged proteins","volume":"2","author":"Moreau","year":"2012","journal-title":"RSC Adv."},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0220","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ab.2008.01.026","article-title":"Escherichia coli biotin protein ligase: characterization and development of a high-throughput assay","volume":"376","author":"Ng","year":"2008","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0225","doi-asserted-by":"crossref","first-page":"4","DOI":"10.2174\/1568026613666131111103149","article-title":"Structure guided design of biotin protein ligase inhibitors for antibiotic discovery","volume":"14","author":"Paparella","year":"2014","journal-title":"Curr. Top. Med. Chem."},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0230","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1038\/nrd2201","article-title":"Drugs for bad bugs: confronting the challenges of antibacterial discovery","volume":"6","author":"Payne","year":"2006","journal-title":"Nat. Rev. Drug Discov."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0235","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.abb.2008.08.021","article-title":"Biotin protein ligase from Candida albicans: expression, purification and development of a novel assay","volume":"479","author":"Pendini","year":"2008","journal-title":"Arch. Biochem. Biophys."},{"issue":"6","key":"10.1016\/j.micres.2017.03.007_bib0240","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1002\/pro.2262","article-title":"Structural characterization of Staphylococcus aureus biotin protein ligase and interaction partners: an antibiotic target","volume":"22","author":"Pendini","year":"2013","journal-title":"Protein Sci."},{"issue":"46","key":"10.1016\/j.micres.2017.03.007_bib0245","doi-asserted-by":"crossref","first-page":"32847","DOI":"10.1074\/jbc.274.46.32847","article-title":"Biotin protein ligase from Saccharomyces cerevisiae: the N-terminal domain is required for complete activity","volume":"274","author":"Polyak","year":"1999","journal-title":"J. Biol. Chem."},{"issue":"5","key":"10.1016\/j.micres.2017.03.007_bib0250","doi-asserted-by":"crossref","first-page":"e2320","DOI":"10.1371\/journal.pone.0002320","article-title":"Ligand specificity of group I biotin protein ligase of Mycobacterium tuberculosis","volume":"3","author":"Purushothaman","year":"2008","journal-title":"PLoS One"},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0255","doi-asserted-by":"crossref","first-page":"e16850","DOI":"10.1371\/journal.pone.0016850","article-title":"Diversity in functional organization of class I and class II biotin protein ligase","volume":"6","author":"Purushothaman","year":"2011","journal-title":"PLoS One"},{"issue":"9","key":"10.1016\/j.micres.2017.03.007_bib0260","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1111\/j.1462-2920.2004.00664.x","article-title":"Uncultured soil bacteria are a reservoir of new antibiotic resistance genes","volume":"6","author":"Riesenfeld","year":"2004","journal-title":"Environ. Microbiol."},{"issue":"10","key":"10.1016\/j.micres.2017.03.007_bib0265","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1101\/gr.314502","article-title":"Conservation of the biotin regulon and the BirA regulatory signal in Eubacteria and Archaea","volume":"12","author":"Rodionov","year":"2002","journal-title":"Genome Res."},{"issue":"14","key":"10.1016\/j.micres.2017.03.007_bib0270","doi-asserted-by":"crossref","first-page":"6461","DOI":"10.1016\/S0021-9258(18)68661-2","article-title":"Evolutionary conservation among biotin enzymes","volume":"263","author":"Samols","year":"1988","journal-title":"J. Biol. Chem."},{"issue":"10","key":"10.1016\/j.micres.2017.03.007_bib0275","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1038\/nbt1093-1138","article-title":"Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli","volume":"11","author":"Schatz","year":"1993","journal-title":"Nat. Biotechnol."},{"issue":"21","key":"10.1016\/j.micres.2017.03.007_bib0280","doi-asserted-by":"crossref","first-page":"17823","DOI":"10.1074\/jbc.M112.356576","article-title":"Selective inhibition of biotin protein ligase from Staphylococcus aureus","volume":"287","author":"Soares da Costa","year":"2012","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0285","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.diagmicrobio.2013.07.009","article-title":"Improved diagnosis of melioidosis using a 2-dimensional immunoarray","volume":"77","author":"Sorenson","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"5","key":"10.1016\/j.micres.2017.03.007_bib0290","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1039\/C4AY02666G","article-title":"In-gel detection of biotin\u2013protein conjugates with a green fluorescent streptavidin probe","volume":"7","author":"Sorenson","year":"2015","journal-title":"Anal. Methods-UK"},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0295","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1006\/jmbi.1999.3086","article-title":"Ligand-linked structural changes in the Escherichia coli biotin repressor: the significance of surface loops for binding and allostery","volume":"292","author":"Streaker","year":"1999","journal-title":"J. Mol. Biol."},{"issue":"9","key":"10.1016\/j.micres.2017.03.007_bib0300","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1039\/c3sc51127h","article-title":"Optimising in situ click chemistry: the screening and identification of biotin protein ligase inhibitors","volume":"4","author":"Tieu","year":"2013","journal-title":"Chem. Sci."},{"issue":"19","key":"10.1016\/j.micres.2017.03.007_bib0305","doi-asserted-by":"crossref","first-page":"4689","DOI":"10.1016\/j.bmcl.2014.08.030","article-title":"Heterocyclic acyl-phosphate bioisostere-based inhibitors of Staphylococcus aureus biotin protein ligase","volume":"24","author":"Tieu","year":"2014","journal-title":"Bioorg. Med. Chem. Lett."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0310","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1021\/ml500475n","article-title":"Improved synthesis of biotinol-5\u2032-AMP: implications for antibacterial discovery","volume":"6","author":"Tieu","year":"2015","journal-title":"ACS Med. Chem. Lett."},{"issue":"1","key":"10.1016\/j.micres.2017.03.007_bib0315","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jmb.2008.12.086","article-title":"Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity","volume":"387","author":"Tron","year":"2009","journal-title":"J. Mol. Biol."},{"issue":"9370","key":"10.1016\/j.micres.2017.03.007_bib0320","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1016\/S0140-6736(03)13374-0","article-title":"Melioidosis","volume":"361","author":"White","year":"2003","journal-title":"Lancet"},{"issue":"4","key":"10.1016\/j.micres.2017.03.007_bib0325","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/nrmicro1385","article-title":"Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei","volume":"4","author":"Wiersinga","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"issue":"19","key":"10.1016\/j.micres.2017.03.007_bib0330","doi-asserted-by":"crossref","first-page":"9257","DOI":"10.1073\/pnas.89.19.9257","article-title":"Escherichia-coli biotin holoenzyme synthetase biorepressor crystal-structure delineates the biotin-Binding and DNA-binding domains","volume":"89","author":"Wilson","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"2","key":"10.1016\/j.micres.2017.03.007_bib0335","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.jmb.2005.12.066","article-title":"Co-repressor induced order and biotin repressor dimerization: a case for divergent followed by convergent evolution","volume":"357","author":"Wood","year":"2006","journal-title":"J. Mol. Biol."},{"issue":"3","key":"10.1016\/j.micres.2017.03.007_bib0340","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nrmicro1614","article-title":"The antibiotic resistome: the nexus of chemical and genetic diversity","volume":"5","author":"Wright","year":"2007","journal-title":"Nat. Rev. Microbiol."},{"issue":"51","key":"10.1016\/j.micres.2017.03.007_bib0345","doi-asserted-by":"crossref","first-page":"16624","DOI":"10.1021\/bi00051a010","article-title":"Evidence for distinct ligand-bound conformational states of the multifunctional Escherichia coli repressor of biotin biosynthesis","volume":"34","author":"Xu","year":"1995","journal-title":"Biochemistry"}],"container-title":["Microbiological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944501317300198?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944501317300198?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T17:26:41Z","timestamp":1658856401000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0944501317300198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":69,"alternative-id":["S0944501317300198"],"URL":"https:\/\/doi.org\/10.1016\/j.micres.2017.03.007","relation":{},"ISSN":["0944-5013"],"issn-type":[{"value":"0944-5013","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Functional characterisation of Burkholderia pseudomallei biotin protein ligase: A toolkit for anti-melioidosis drug development","name":"articletitle","label":"Article Title"},{"value":"Microbiological Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micres.2017.03.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier GmbH.","name":"copyright","label":"Copyright"}]}}