{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:29:34Z","timestamp":1778624974480,"version":"3.51.4"},"reference-count":82,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04293\/2020"],"award-info":[{"award-number":["UIDB\/04293\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/50016\/2025"],"award-info":[{"award-number":["UID\/50016\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001704","name":"European Society of Clinical Microbiology and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbiological Research"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.micres.2026.128442","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T17:10:36Z","timestamp":1768324236000},"page":"128442","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Automated high-throughput microscopy screening unveiled new Listeria monocytogenes genes involved in cell infection"],"prefix":"10.1016","volume":"306","author":[{"given":"\u00c2ngela","family":"Alves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Meireles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Suriano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rute","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz G.","family":"Bernardes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Pombinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4001-1332","authenticated-orcid":false,"given":"Didier","family":"Cabanes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micres.2026.128442_bib1","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1126\/science.1116008","article-title":"Genome-wide RNAi screen for host factors required for intracellular bacterial infection","volume":"309","author":"Agaisse","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.micres.2026.128442_bib2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1600-0854.2007.00683.x","article-title":"Characterization of a Listeria monocytogenes protein interfering with Rab5a","volume":"9","author":"Alvarez-Dominguez","year":"2008","journal-title":"Traffic"},{"key":"10.1016\/j.micres.2026.128442_bib3","doi-asserted-by":"crossref","first-page":"6887","DOI":"10.1128\/AEM.70.11.6887-6891.2004","article-title":"New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria","volume":"70","author":"Arnaud","year":"2004","journal-title":"Appl. Environ. Microbiol"},{"key":"10.1016\/j.micres.2026.128442_bib4","doi-asserted-by":"crossref","first-page":"496","DOI":"10.2478\/s11658-010-0019-z","article-title":"Integrin receptors play a role in the internalin B-dependent entry of Listeria monocytogenes into host cells","volume":"15","author":"Auriemma","year":"2010","journal-title":"Cell Mol. Biol. Lett."},{"key":"10.1016\/j.micres.2026.128442_bib5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bbamcr.2011.06.007","article-title":"ClpXP, an ATP-powered unfolding and protein-degradation machine","volume":"1823","author":"Baker","year":"2012","journal-title":"Biochim. Biophys. Acta (BBA) Mol. Cell Res."},{"key":"10.1016\/j.micres.2026.128442_bib6","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.1128\/AEM.02612-09","article-title":"Single-cell techniques using chromosomally tagged fluorescent bacteria to study Listeria monocytogenes infection processes","volume":"76","author":"Balestrino","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib7","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1039\/C6SC03438A","article-title":"Insights into ClpXP proteolysis: heterooligomerization and partial deactivation enhance chaperone affinity and substrate turnover in Listeria monocytogenes","volume":"8","author":"Balogh","year":"2017","journal-title":"Chem. Sci."},{"key":"10.1016\/j.micres.2026.128442_bib8","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1039\/D2CB00077F","article-title":"Listeria monocytogenes utilizes the ClpP1\/2 proteolytic machinery for fine-tuned substrate degradation at elevated temperatures","volume":"3","author":"Balogh","year":"2022","journal-title":"RSC Chem. Biol."},{"key":"10.1016\/j.micres.2026.128442_bib9","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/BF00331554","article-title":"Occurrence of a regulatory deficiency in purine biosynthesis among purA mutants of Salmonella typhimurium","volume":"145","author":"Benson","year":"1976","journal-title":"MGG Mol. Gen. Genet."},{"key":"10.1016\/j.micres.2026.128442_bib10","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1261\/rna.1796210","article-title":"Autoregulatory systems controlling translation factor expression: thermostat-like control of translational accuracy","volume":"16","author":"Betney","year":"2010","journal-title":"RNA"},{"key":"10.1016\/j.micres.2026.128442_bib11","article-title":"Bacterial plasminogen receptors utilize host plasminogen system for effective invasion and dissemination","volume":"2012","author":"Bhattacharya","year":"2012","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.micres.2026.128442_bib12","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1046\/j.1365-2958.1999.01560.x","article-title":"The 213-amino-acid leucine-rich repeat region of the Listeria monocytogenes InlB protein is sufficient for entry into mammalian cells, stimulation of PI 3-kinase and membrane ruffling","volume":"34","author":"Braun","year":"1999","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib13","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1128\/IAI.00837-12","article-title":"Genome-wide identification of genes required for fitness of group a streptococcus in human blood","volume":"81","author":"Breton","year":"2013","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib14","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1128\/iai.1.5.446-454.1970","article-title":"Interconversion of purine mononucleotides in Pasteurella pestis","volume":"1","author":"Brubaker","year":"1970","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000449","article-title":"In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection","volume":"5","author":"Camejo","year":"2009","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micres.2026.128442_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004919","article-title":"L-Rhamnosylation of Listeria monocytogenes wall teichoic acids promotes resistance to antimicrobial peptides by delaying interaction with the membrane","volume":"11","author":"Carvalho","year":"2015","journal-title":"PLOS Pathog."},{"key":"10.1016\/j.micres.2026.128442_bib17","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.1111\/1462-2920.14351","article-title":"l-Rhamnosylation of wall teichoic acids promotes efficient surface association of Listeria monocytogenes virulence factors InlB and Ami through interaction with GW domains","volume":"20","author":"Carvalho","year":"2018","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib18","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1073\/pnas.012363899","article-title":"Hpt, a bacterial homolog of the microsomal glucose-6-phosphate translocase, mediates rapid intracellular proliferation in Listeria","volume":"99","author":"Chico-Calero","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.micres.2026.128442_bib19","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1111\/j.1365-2672.2011.04982.x","article-title":"A role for proline synthesis and transport in Listeria monocytogenes barotolerance","volume":"110","author":"Considine","year":"2011","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib20","doi-asserted-by":"crossref","first-page":"3925","DOI":"10.1128\/iai.64.9.3925-3929.1996","article-title":"Iron availability affects entry of Listeria monocytogenes into the enterocytelike cell line Caco-2","volume":"64","author":"Conte","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib21","doi-asserted-by":"crossref","first-page":"19484","DOI":"10.1073\/pnas.1112371108","article-title":"Illuminating the landscape of host-pathogen interactions with the bacterium Listeria monocytogenes","volume":"108","author":"Cossart","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.micres.2026.128442_bib22","doi-asserted-by":"crossref","first-page":"6840","DOI":"10.1128\/JB.181.21.6840-6843.1999","article-title":"Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in Listeria monocytogenes","volume":"181","author":"Cotter","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micres.2026.128442_bib23","doi-asserted-by":"crossref","first-page":"2784","DOI":"10.1128\/AAC.46.9.2784-2790.2002","article-title":"The LisRK signal transduction system determines the sensitivity of Listeria monocytogenes to nisin and cephalosporins","volume":"46","author":"Cotter","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.micres.2026.128442_bib24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/322273a0","article-title":"Expression of peptide chain release factor 2 requires high-efficiency frameshift","volume":"322","author":"Craigen","year":"1986","journal-title":"Nature"},{"key":"10.1016\/j.micres.2026.128442_bib25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0075437","article-title":"A mariner transposon-based signature-tagged mutagenesis system for the analysis of oral infection by Listeria monocytogenes.","volume":"8","author":"Cummins","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.micres.2026.128442_bib26","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1365-2958.1995.tb02297.x","article-title":"Entry of Listeria monocytogenes into hepatocytes requires expression of InIB, a surface protein of the internalin multigene family","volume":"16","author":"Dramsi","year":"1995","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib27","doi-asserted-by":"crossref","unstructured":"Faith, N.G., Kim, J.W., Azizoglu, R., Kathariou, S., Czuprynski, C., 2012. Purine biosynthesis mutants (purA and purB) of serotype 4b Listeria monocytogenes are severely attenuated for systemic infection in intragastrically inoculated A\/j mice. \u3008https:\/\/home.liebertpub.com\/fpd\u3009 9, 480\u2013486. https:\/\/doi.org\/10.1089\/FPD.2011.1013.","DOI":"10.1089\/fpd.2011.1013"},{"key":"10.1016\/j.micres.2026.128442_bib28","doi-asserted-by":"crossref","DOI":"10.1128\/iai.00343-25","article-title":"Listeria monocytogenes adenosine auxotrophs are impaired for intracellular and extracellular growth but retain potent immunogenicity","author":"Feng","year":"2025","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib29","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1101\/gr.276747.122","article-title":"Listeria monocytogenes genes supporting growth under standard laboratory cultivation conditions and during macrophage infection","volume":"32","author":"Fischer","year":"2022","journal-title":"Genome Res."},{"key":"10.1016\/j.micres.2026.128442_bib30","article-title":"The European union one health 2023 zoonoses report","volume":"22","author":"Food Safety Authority","year":"2024","journal-title":"EFSA J."},{"key":"10.1016\/j.micres.2026.128442_bib31","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/0092-8674(91)90009-N","article-title":"Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci","volume":"65","author":"Gaillard","year":"1991","journal-title":"Cell"},{"key":"10.1016\/j.micres.2026.128442_bib32","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.13311","article-title":"Mycobacterium tuberculosis glyceraldehyde-3-phosphate dehydrogenase plays a dual role\u2014as an adhesin and as a receptor for plasmin(ogen)","volume":"23","author":"Gani","year":"2021","journal-title":"Cell Microbiol."},{"issue":"1979","key":"10.1016\/j.micres.2026.128442_bib33","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1126\/science.1063447","article-title":"Comparative genomics of Listeria Species","volume":"294","author":"Glaser","year":"2001","journal-title":"Science"},{"key":"10.1016\/j.micres.2026.128442_bib34","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00104-20","article-title":"De novo purine biosynthesis is required for intracellular growth of Staphylococcus aureus and for the hypervirulence phenotype of a purr mutant","volume":"88","author":"Goncheva","year":"2020","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib35","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.01505","article-title":"The \u03c3B-mediated general stress response of Listeria monocytogenes: life and death decision making in a pathogen","volume":"11","author":"Guerreiro","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib36","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00751-19","article-title":"Mild stress conditions during laboratory culture promote the proliferation of mutations that negatively affect sigma B activity in Listeria monocytogenes","volume":"202","author":"Guerreiro","year":"2020","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micres.2026.128442_bib37","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1006\/mpat.2001.0451","article-title":"Escherichia coli K1 pur A and sor C are preferentially expressed upon association with human brain microvascular endothelial cells","volume":"31","author":"Hoffman","year":"2001","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micres.2026.128442_bib38","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.fsi.2022.04.001","article-title":"PurA facilitates Edwardsiella piscicida to escape NF-\u03baB signaling activation","volume":"124","author":"Hu","year":"2022","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.micres.2026.128442_bib39","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1099\/00221287-53-2-147","article-title":"The growth of purine mutants of Bacillus anthracis in the body of the mouse","volume":"53","author":"Iv\u00e1novics","year":"1968","journal-title":"J. Gen. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib40","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1046\/j.1365-2958.1999.01652.x","article-title":"Interaction between the protein InlB of Listeria monocytogenes and lipoteichoic acid: a novel mechanism of protein association at the surface of Gram-positive bacteria","volume":"34","author":"Jonqui\u00e8res","year":"1999","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib41","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3389\/fcimb.2020.00089","article-title":"Diverse localization and protein binding abilities of glyceraldehyde-3-phosphate dehydrogenase in pathogenic bacteria: the key to its multifunctionality?","volume":"10","author":"Kopeckova","year":"2020","journal-title":"Front Cell Infect. Microbiol"},{"key":"10.1016\/j.micres.2026.128442_bib42","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.tim.2004.12.003","article-title":"Bacterial metastasis: the host plasminogen system in bacterial invasion","volume":"13","author":"L\u00e4hteenm\u00e4ki","year":"2005","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib43","doi-asserted-by":"crossref","first-page":"5470","DOI":"10.1002\/j.1460-2075.1996.tb00930.x","article-title":"A purified mariner transposase is sufficient to mediate transposition in vitro","volume":"15","author":"Lampe","year":"1996","journal-title":"EMBO J."},{"key":"10.1016\/j.micres.2026.128442_bib44","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/genetics\/149.1.179","article-title":"Factors affecting transposition of the Himar1 mariner transposon in vitro","volume":"149","author":"Lampe","year":"1998","journal-title":"Genetics"},{"key":"10.1016\/j.micres.2026.128442_bib45","doi-asserted-by":"crossref","first-page":"3068","DOI":"10.1128\/JB.00928-09","article-title":"Golden pigment production and virulence gene expression are affected by metabolisms in Staphylococcus aureus","volume":"192","author":"Lan","year":"2010","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micres.2026.128442_bib46","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1099\/mic.0.2006\/000620-0","article-title":"Molecular characterization of the Fur protein of Listeria monocytogenes","volume":"153","author":"Ledala","year":"2007","journal-title":"Microbiology"},{"key":"10.1016\/j.micres.2026.128442_bib47","doi-asserted-by":"crossref","first-page":"48569","DOI":"10.1074\/jbc.M408759200","article-title":"Promiscuous target interactions in the mariner transposon himar1","volume":"279","author":"Lipkow","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.micres.2026.128442_bib48","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.chom.2020.10.001","article-title":"Exploration of bacterial bottlenecks and Streptococcus pneumoniae pathogenesis by CRISPRi-Seq","volume":"29","author":"Liu","year":"2021","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.micres.2026.128442_bib49","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.ijmm.2018.08.006","article-title":"Uropathogenic Escherichia coli preferentially utilize metabolites in urine for nucleotide biosynthesis through salvage pathways","volume":"308","author":"Ma","year":"2018","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib50","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1046\/j.1365-2958.2000.01708.x","article-title":"pH-regulated activation and release of a bacteria-associated phospholipase C during intracellular infection by Listeria monocytogenes","volume":"35","author":"Marquis","year":"2000","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib51","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0882-4010(87)90071-4","article-title":"Effect of different purine auxotrophic mutations on mouse-virulence of a Vi-positive strain of Salmonella dublin and of two strains of Salmonella typhimurium","volume":"3","author":"McFarland","year":"1987","journal-title":"Micro Pathog."},{"key":"10.1016\/j.micres.2026.128442_bib52","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.1128\/iai.64.12.5430-5433.1996","article-title":"Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin","volume":"64","author":"Mengaud","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib53","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1099\/00221287-146-3-731","article-title":"Identification of new loci involved in adhesion of Listeria monocytogenes to eukaryotic cells","volume":"146","author":"Milohanic","year":"2000","journal-title":"Microbiology"},{"key":"10.1016\/j.micres.2026.128442_bib54","doi-asserted-by":"crossref","first-page":"3921","DOI":"10.1128\/AEM.00314-08","article-title":"Tools for functional postgenomic analysis of Listeria monocytogenes","volume":"74","author":"Monk","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib55","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.1007657","article-title":"Identification of genetic elements required for Listeria monocytogenes growth under limited nutrient conditions and virulence by a screening of transposon insertion library","volume":"13","author":"Narayanan","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AEM.02349-20","article-title":"Biofilm formation by Listeria monocytogenes 15G01, a persistent isolate from a seafood-processing plant, is influenced by inactivation of multiple genes belonging to different functional groups","volume":"87","author":"Nowak","year":"2021","journal-title":"Appl. Environ. Microbiol"},{"key":"10.1016\/j.micres.2026.128442_bib57","doi-asserted-by":"crossref","first-page":"4216","DOI":"10.1128\/AEM.00031-10","article-title":"Listeria monocytogenes \u03c3B has a small core regulon and a conserved role in virulence but makes differential contributions to stress tolerance across a diverse collection of strains","volume":"76","author":"Oliver","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib58","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/1348-0421.12030","article-title":"Fibronectin-binding protein, FbpA, is the adhesin responsible for pathogenesis of Listeria monocytogenes infection","volume":"57","author":"Osanai","year":"2013","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.micres.2026.128442_bib59","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02095-21","article-title":"The lipoteichoic acid-related proteins YqgS and LafA contribute to the resistance of Listeria monocytogenes to Nisin","volume":"10","author":"Pang","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micres.2026.128442_bib60","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0378-1119(90)90479-B","article-title":"High-efficiency transformation of Listeria monocytogenes by electroporation of penicillin-treated cells","volume":"94","author":"Park","year":"1990","journal-title":"Gene"},{"key":"10.1016\/j.micres.2026.128442_bib61","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02580-16","article-title":"Novel cadmium resistance determinant in Listeria monocytogenes","volume":"83","author":"Parsons","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib62","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01283-17","article-title":"Chitinase expression in Listeria monocytogenes is influenced by lmo0327, which encodes an internalin-like protein","volume":"83","author":"Paspaliari","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib63","doi-asserted-by":"crossref","first-page":"866","DOI":"10.3109\/1040841X.2015.1080214","article-title":"Bacterial pathogens activate plasminogen to breach tissue barriers and escape from innate immunity","volume":"42","author":"Peetermans","year":"2016","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib64","first-page":"113","article-title":"ActA of Listeria monocytogenes and its manifold activities as an important listerial virulence factor","volume":"399","author":"Pillich","year":"2017","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.micres.2026.128442_bib65","doi-asserted-by":"crossref","first-page":"a010009","DOI":"10.1101\/cshperspect.a010009","article-title":"Entry of Listeria monocytogenes in mammalian epithelial cells: an updated view","volume":"2","author":"Pizarro-Cerd\u00e1","year":"2012","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.micres.2026.128442_bib66","doi-asserted-by":"crossref","first-page":"5620","DOI":"10.1128\/IAI.66.12.5620-5629.1998","article-title":"Large-scale identification of virulence genes from Streptococcus pneumoniae","volume":"66","author":"Polissi","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib67","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1128\/AEM.00626-15","article-title":"Two-component-system histidine kinases involved in growth of Listeria monocytogenes EGD-e at low temperatures","volume":"81","author":"P\u00f6ntinen","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib68","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/nrmicro.2017.126","article-title":"Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis","volume":"16","author":"Radoshevich","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib69","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nrmicro2112","article-title":"Life on the inside: the intracellular lifestyle of cytosolic bacteria","volume":"7","author":"Ray","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib70","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1128\/IAI.72.2.717-727.2004","article-title":"Disruption of putative regulatory loci in Listeria monocytogenes demonstrates a significant role for fur and PerR in virulence","volume":"72","author":"Rea","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib71","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1086\/654880","article-title":"LapB, a novel Listeria monocytogenes LPXTG surface adhesin, required for entry into eukaryotic cells and virulence","volume":"202","author":"Reis","year":"2010","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.micres.2026.128442_bib72","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nature14029","article-title":"Glutathione activates virulence gene expression of an intracellular pathogen","volume":"517","author":"Reniere","year":"2015","journal-title":"Nature"},{"key":"10.1016\/j.micres.2026.128442_bib73","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1186\/1471-2164-11-573","article-title":"Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling","volume":"11","author":"Schauer","year":"2010","journal-title":"BMC Genom."},{"key":"10.1016\/j.micres.2026.128442_bib74","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.GPP3-0014-2018","article-title":"Epidemiology and clinical manifestations of Listeria monocytogenes infection","volume":"7","author":"Schlech","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.micres.2026.128442_bib75","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.1128\/AEM.67.6.2571-2577.2001","article-title":"Identification and disruption of the proBA Locus in Listeria monocytogenes: role of proline biosynthesis in salt tolerance and murine infection","volume":"67","author":"Sleator","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.micres.2026.128442_bib76","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1111\/j.1469-0691.2005.01176.x","article-title":"A novel role for the LisRK two-component regulatory system in listerial osmotolerance","volume":"11","author":"Sleator","year":"2005","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.micres.2026.128442_bib77","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.1128\/iai.63.11.4231-4237.1995","article-title":"The two distinct phospholipases C of Listeria monocytogenes have overlapping roles in escape from a vacuole and cell-to-cell spread","volume":"63","author":"Smith","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micres.2026.128442_bib78","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1128\/JB.00091-11","article-title":"Atp requirement for acidic resistance in Escherichia coli.","volume":"193","author":"Sun","year":"2011","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micres.2026.128442_bib79","doi-asserted-by":"crossref","first-page":"6943","DOI":"10.1128\/AEM.69.11.6943-6945.2003","article-title":"Development of a synthetic minimal medium for Listeria monocytogenes","volume":"69","author":"Tsai","year":"2003","journal-title":"Appl. Environ. Microbiol"},{"key":"10.1016\/j.micres.2026.128442_bib80","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/path.1711760208","article-title":"Altered expression and function of E-cadherin in cervical intraepithelial neoplasia and invasive squamous cell carcinoma","volume":"176","author":"Vessey","year":"1995","journal-title":"J. Pathol."},{"key":"10.1016\/j.micres.2026.128442_bib81","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2221047120","article-title":"Carbon dioxide\/bicarbonate is required for sensitive inactivation of mammalian glyceraldehyde-3-phosphate dehydrogenase by hydrogen peroxide","volume":"120","author":"Winterbourn","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.micres.2026.128442_bib82","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.1128\/JB.00016-09","article-title":"Development of a mariner-based transposon and identification of Listeria monocytogenes determinants, including the peptidyl-prolyl isomerase PrsA2, that contribute to its hemolytic phenotype","volume":"191","author":"Zemansky","year":"2009","journal-title":"J. Bacteriol."}],"container-title":["Microbiological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944501326000066?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0944501326000066?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:02:55Z","timestamp":1778623375000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0944501326000066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":82,"alternative-id":["S0944501326000066"],"URL":"https:\/\/doi.org\/10.1016\/j.micres.2026.128442","relation":{},"ISSN":["0944-5013"],"issn-type":[{"value":"0944-5013","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Automated high-throughput microscopy screening unveiled new Listeria monocytogenes genes involved in cell infection","name":"articletitle","label":"Article Title"},{"value":"Microbiological Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micres.2026.128442","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"128442"}}