{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:26:00Z","timestamp":1760887560715,"version":"3.44.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2005,3]]},"DOI":"10.1016\/j.mimet.2004.10.018","type":"journal-article","created":{"date-parts":[[2004,12,6]],"date-time":"2004-12-06T14:30:37Z","timestamp":1102343437000},"page":"383-393","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Isolation of Brucella abortus total RNA from B. abortus-infected murine RAW macrophages"],"prefix":"10.1016","volume":"60","author":[{"given":"Jill","family":"Covert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Eskra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Splitter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2004.10.018_bib1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1093\/oxfordjournals.jbchem.a129936","article-title":"Studies on SDS-phenol methods for extraction of rat liver nuclear RNA: II. Polyacrylamide gel electrophoresis of nuclear RNA obtained using various conditions for SDS-phenol extraction","volume":"72","author":"Abe","year":"1972","journal-title":"J. Biochem. (Tokyo)"},{"key":"10.1016\/j.mimet.2004.10.018_bib2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0166-0934(98)00082-2","article-title":"A comparison of two RNA isolation methods for double-stranded RNA of infectious bursal disease virus","volume":"74","author":"Akin","year":"1998","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mimet.2004.10.018_bib3","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1128\/IAI.68.7.4255-4263.2000","article-title":"Intracellular trafficking of Brucella abortus in J774 macrophages","volume":"68","author":"Arenas","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib4","doi-asserted-by":"crossref","first-page":"9697","DOI":"10.1073\/pnas.112318199","article-title":"Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays","volume":"99","author":"Bernstein","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mimet.2004.10.018_bib5","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0167-7012(03)00175-1","article-title":"Computational methods and evaluation of RNA stabilization reagents for genome-wide expression studies","volume":"55","author":"Bhagwat","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2004.10.018_bib6","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis","volume":"4","author":"Boschiroli","year":"2001","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib36","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1006\/abio.1994.1528","article-title":"A method to isolate RNA from gram-positive bacteria and mycobacteria","volume":"222","author":"Cheung","year":"1994","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mimet.2004.10.018_bib8","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1749-6632.2000.tb05285.x","article-title":"Development, testing and commercialization of a new Brucellosis vaccine for cattle","volume":"916","author":"Cheville","year":"2000","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"10.1016\/j.mimet.2004.10.018_bib9","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1128\/jb.176.1.198-205.1994","article-title":"Translation initiation factor IF1 is essential for cell viability in Escherichia coli","volume":"176","author":"Cummings","year":"1994","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2004.10.018_bib10","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0378-1135(02)00233-X","article-title":"Brucella vaccines in wildlife","volume":"90","author":"Davis","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib39","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/nbt0198-45","article-title":"Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays","volume":"16","author":"de Saizieu","year":"1998","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mimet.2004.10.018_bib11","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1574-6968.2000.tb09100.x","article-title":"Isolation of RNA from mycobacteria grown under in vitro and in vivo conditions","volume":"186","author":"Dietrich","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2004.10.018_bib12","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1046\/j.1365-2958.2003.03313.x","article-title":"Unravelling the biology of macrophage infection by gene expression profiling of intracellular Salmonella enterica","volume":"47","author":"Eriksson","year":"2003","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib13","doi-asserted-by":"crossref","first-page":"7736","DOI":"10.1128\/IAI.69.12.7736-7742.2001","article-title":"Brucella abortus genes identified following constitutive growth and macrophage infection","volume":"69","author":"Eskra","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib14","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1128\/IAI.68.1.257-263.2000","article-title":"Effects of opsonization and gamma interferon on growth of Brucella melitensis 16M in mouse peritoneal macrophages in vitro","volume":"68","author":"Eze","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0378-1135(01)00460-6","article-title":"Development of a Brucella suis specific hybridisation probe and PCR which distinguishes B. suis from Brucella abortus","volume":"84","author":"Fayazi","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib16","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1128\/IAI.68.3.1297-1303.2000","article-title":"Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis","volume":"68","author":"Foulongne","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib17","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1128\/IAI.68.1.342-351.2000","article-title":"In vitro Brucella suis infection prevents the programmed cell death of human monocytic cells","volume":"68","author":"Gross","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib18","doi-asserted-by":"crossref","first-page":"4073","DOI":"10.1021\/ac000428g","article-title":"In-situ sampling and separation of RNA from individual mammalian cells","volume":"72","author":"Han","year":"2000","journal-title":"Anal. Chem."},{"key":"10.1016\/j.mimet.2004.10.018_bib19","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/iai.61.1.124-134.1993","article-title":"Effects of cytokines on intracellular growth of Brucella abortus","volume":"61","author":"Jiang","year":"1993","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib20","doi-asserted-by":"crossref","first-page":"6695","DOI":"10.1128\/IAI.67.12.6695-6697.1999","article-title":"Constitutive and inducible expression of green fluorescent protein in Brucella suis","volume":"67","author":"Kohler","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib21","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1128\/iai.63.4.1409-1414.1995","article-title":"Protein synthesis in Brucella abortus induced during macrophage infection","volume":"63","author":"Lin","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib22","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0378-1135(02)00241-9","article-title":"Brucellosis in Mexico: current status and trends","volume":"90","author":"Luna-Martinez","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib23","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/S0167-7012(02)00218-X","article-title":"A rapid method for RNA preparation from Gram-positive bacteria","volume":"52","author":"Oh","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2004.10.018_bib24","doi-asserted-by":"crossref","first-page":"5711","DOI":"10.1128\/IAI.66.12.5711-5724.1998","article-title":"Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes","volume":"66","author":"Pizarro-Cerda","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib25","doi-asserted-by":"crossref","first-page":"4041","DOI":"10.1128\/IAI.67.8.4041-4047.1999","article-title":"Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages","volume":"67","author":"Porte","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib26","doi-asserted-by":"crossref","first-page":"5274","DOI":"10.1128\/iai.64.12.5274-5283.1996","article-title":"Identification and comparison of macrophage-induced proteins and proteins induced under various stress conditions in Brucella abortus","volume":"64","author":"Rafie-Kolpin","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib27","doi-asserted-by":"crossref","first-page":"5529","DOI":"10.1093\/nar\/gkf682","article-title":"Genome-wide analysis of mRNA decay in resting and activated primary human T lymphocytes","volume":"30","author":"Raghavan","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2004.10.018_bib35","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/j.1472-765X.1995.tb00995.x","article-title":"A rapid protocol for isolation of RNA from mycobacteria","volume":"21","author":"Rajagopalan","year":"1995","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib28","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1128\/IAI.69.6.3995-4006.2001","article-title":"Intracellular survival of Brucella spp. in human monocytes involves conventional uptake but special phagosomes","volume":"69","author":"Rittig","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2004.10.018_bib29","doi-asserted-by":"crossref","first-page":"291","DOI":"10.5858\/2002-126-0291-TMLSRI","article-title":"The microbiology laboratory's role in response to bioterrorism","volume":"126","author":"Robinson-Dunn","year":"2002","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.mimet.2004.10.018_bib40","first-page":"123","article-title":"Improved quantitation and reproducibility in Mycobacterium tuberculosis DNA microarrays","volume":"4","author":"Schroeder","year":"2002","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2004.10.018_bib30","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2004.10.018_bib34","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1172\/JCI0215268","article-title":"mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils","volume":"110","author":"Staudinger","year":"2002","journal-title":"J. Clint. Invest."},{"key":"10.1016\/j.mimet.2004.10.018_bib38","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/76543","article-title":"Genome-directed primers for selective labeling of bacterial transcripts for DNA microarray analysis","volume":"18","author":"Talaat","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mimet.2004.10.018_bib31","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/j.1574-6968.1998.tb12906.x","article-title":"In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes","volume":"160","author":"Wagner","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2004.10.018_bib32","first-page":"2.4.1","article-title":"Preparation of genomic DNA from bacteria","volume":"vol. 1","author":"Wilson","year":"1998"},{"key":"10.1016\/j.mimet.2004.10.018_bib37","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0167-7012(02)00078-7","article-title":"Efficient isolation of total RNA from antibiotic-producing bacterium Amycolaptosis mediterranei","volume":"51","author":"Yao","year":"2002","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2004.10.018_bib33","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1039\/b000368i","article-title":"Rapid isolation method for lipopolysaccharide and lipid A from gram-negative bacteria","volume":"125","author":"Yi","year":"2000","journal-title":"Analyst"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770120400301X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770120400301X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:32:23Z","timestamp":1759102343000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016770120400301X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["S016770120400301X"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2004.10.018","relation":{},"ISSN":["0167-7012"],"issn-type":[{"type":"print","value":"0167-7012"}],"subject":[],"published":{"date-parts":[[2005,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation of Brucella abortus total RNA from B. abortus-infected murine RAW macrophages","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2004.10.018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2004 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}