{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:22:42Z","timestamp":1792189362242,"version":"4.4.0"},"reference-count":24,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2007,1]]},"DOI":"10.1016\/j.mimet.2006.07.011","type":"journal-article","created":{"date-parts":[[2006,9,13]],"date-time":"2006-09-13T07:16:35Z","timestamp":1158131795000},"page":"178-183","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development of a new real-time TaqMan PCR assay for quantitative analyses of Candida albicans resistance genes expression"],"prefix":"10.1016","volume":"68","author":[{"given":"Grzegorz","family":"Kofla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Ruhnke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2006.07.011_bib1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/AAC.41.3.535","article-title":"Reversible fluconazole resistance in Candida albicans: a potential in vitro model","volume":"41","author":"Calvet","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2006.07.011_bib2","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1046\/j.1365-2958.2002.02814.x","article-title":"A common drug-responsive element mediates the upregulation of the Candida albicans ABC transporters CDR1 and CDR2, two genes involved in antifungal drug resistance","volume":"43","author":"De Micheli","year":"2002","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib3","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1086\/520192","article-title":"Nosocomial bloodstream infections in United States hospitals: a three-year analysis","volume":"29","author":"Edmond","year":"1999","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.mimet.2006.07.011_bib4","unstructured":"Fast Prep Instruction Manual, Qbiogene, 2003."},{"key":"10.1016\/j.mimet.2006.07.011_bib5","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1128\/JCM.42.5.2085-2093.2004","article-title":"Rapid quantification of drug resistance gene expression in Candida albicans by reverse transcriptase LightCycler PCR and fluorescent probe hybridization","volume":"42","author":"Frade","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib6","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1128\/AAC.42.12.3065","article-title":"Multiple molecular mechanisms contribute to a stepwise development of fluconazole resistance in clinical Candida albicans strains","volume":"42","author":"Franz","year":"1998","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2006.07.011_bib7","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1128\/jcm.26.9.1874-1877.1988","article-title":"Improved method for azole antifungal susceptibility testing","volume":"26","author":"Gordon","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib8","doi-asserted-by":"crossref","first-page":"3064","DOI":"10.1128\/AAC.48.8.3064-3079.2004","article-title":"Comparison of gene expression profiles of Candida albicans azole resistant clinical isolates and laboratory strains exposed to drug inducing multidrug transporters","volume":"48","author":"Karababa","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2006.07.011_bib9","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1128\/JCM.38.2.586-590.2000","article-title":"Quantification of fungal DNA by using fluorescence resonance energy transfer and LightCycler system","volume":"38","author":"Loeffler","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib10","doi-asserted-by":"crossref","first-page":"3293","DOI":"10.1128\/JCM.41.7.3293-3298.2003","article-title":"Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay","volume":"41","author":"Maaroufi","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib11","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1128\/JCM.42.7.3159-3163.2004","article-title":"Comparison of different methods of isolation of DNA of commonly encountered Candida species and its quantitation by using a real-time PCR-based assay","volume":"42","author":"Maaroufi","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib12","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/S1525-1578(10)60498-9","article-title":"Early detection and identification of commonly encountered Candida species from simulated blood cultures by using a real-time PCR-based assay","volume":"6","author":"Maaroufi","year":"2004","journal-title":"J. Mol. Diagnostics"},{"key":"10.1016\/j.mimet.2006.07.011_bib13","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1002\/jnr.490350607","article-title":"Reverse transcription and polymerase chain reaction technique for quantification of mRNA in primary astrocyte cultures","volume":"15","author":"Murphy","year":"1993","journal-title":"J. Neurosci. Res."},{"key":"10.1016\/j.mimet.2006.07.011_bib14","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1211\/0022357022359","article-title":"Exposure of the yeast Candida albicans to the anti-neoplastic agent adriamycin increases the tolerance to amphotericin B","volume":"55","author":"O'Keeffe","year":"2003","journal-title":"J. Pharm. Pharmacol."},{"key":"10.1016\/j.mimet.2006.07.011_bib15","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0732-8893(97)00240-X","article-title":"National surveillance of nosocomial blood stream infection due to Candida albicans: frequency of occurrence and anti-fungal susceptibility in the SCOPE programme","volume":"31","author":"Pfaller","year":"1998","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2006.07.011_bib16","unstructured":"Perkin-Elmer, User Bulletin # 2, 1997, DNA\/RNA real-time quantitative PCR, Perkin-Elmer, 1998."},{"key":"10.1016\/j.mimet.2006.07.011_bib17","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S0732-8893(03)00139-1","article-title":"Real-time automated polymerase chain reaction (PCR) to detect Candida albicans and Aspergillus fumigatus DNA in whole blood from high risk patients","volume":"47","author":"Pryce","year":"2003","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2006.07.011_bib18","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.1128\/jcm.32.9.2092-2098.1994","article-title":"Emergence of fluconazole-resistant strains of Candida albicans in patients with recurrent oropharyngeal candidosis and human immunodeficiency virus infection","volume":"32","author":"Ruhnke","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2006.07.011_bib19","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1128\/AAC.39.11.2378","article-title":"Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters","volume":"39","author":"Sanglard","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2006.07.011_bib21","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1128\/AAC.40.10.2300","article-title":"Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors","volume":"40","author":"Sanglard","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2006.07.011_bib20","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1099\/00221287-143-2-405","article-title":"Cloning of Candida albicans genes conferring resistance to azole antifungal agents: characterisation of CDR2, a new multidrug ABC transporter gene","volume":"143","author":"Sanglard","year":"1997","journal-title":"Microbiology"},{"key":"10.1016\/j.mimet.2006.07.011_bib22","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.1128\/AAC.47.8.2404-2412.2003","article-title":"Candida albicans mutations in the ergosterol biosynthetic pathway and resistance to several antifungal agents","volume":"47","author":"Sanglard","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2006.07.011_bib23","doi-asserted-by":"crossref","first-page":"9717","DOI":"10.1073\/pnas.86.24.9717","article-title":"Quantification of mRNA by the polymerase chain reaction","volume":"86","author":"Wang","year":"1989","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mimet.2006.07.011_bib24","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1128\/AAC.41.7.1488","article-title":"Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus","volume":"41","author":"White","year":"1997","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701206002223?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701206002223?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:34:55Z","timestamp":1759102495000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701206002223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["S0167701206002223"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2006.07.011","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2007,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a new real-time TaqMan PCR assay for quantitative analyses of Candida albicans resistance genes expression","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2006.07.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}