{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:44:48Z","timestamp":1791114288356,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2011,1]]},"DOI":"10.1016\/j.mimet.2010.10.021","type":"journal-article","created":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T04:14:19Z","timestamp":1288584859000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"title":["A comparison between polymerase chain reaction (PCR) and traditional techniques for the diagnosis of leptospirosis in bovines"],"prefix":"10.1016","volume":"84","author":[{"given":"Patricia","family":"Hern\u00e1ndez-Rodr\u00edguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar A.","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto A.","family":"Dalmau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladys M.","family":"Quintero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2010.10.021_bb0005","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and leptospirosis","volume":"140","author":"Adler","year":"2010","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0010","doi-asserted-by":"crossref","first-page":"216","DOI":"10.7705\/biomedica.v26i2.1411","article-title":"Evaluaci\u00f3n de la prueba de inmunofluorescencia indirecta para el diagn\u00f3stico de Leptospirosis humana","volume":"26","author":"Agudelo","year":"2006","journal-title":"Biom\u00e9dica"},{"key":"10.1016\/j.mimet.2010.10.021_bb0015","first-page":"1","article-title":"Epidemiolog\u00eda y diagnostic de la Leptospirosis como fundamentos para el dise\u00f1o de estrategias de control","volume":"6","author":"Alfaro","year":"2004","journal-title":"Rev. Digit. CENIAP HOY"},{"key":"10.1016\/j.mimet.2010.10.021_bb0020","doi-asserted-by":"crossref","first-page":"340","DOI":"10.4269\/ajtmh.1995.52.340","article-title":"Evaluation of the immunofluorescent antibody test for the diagnosis of human leptospirosis","volume":"52","author":"Appassakij","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.mimet.2010.10.021_bb0025","first-page":"10","article-title":"Leptospirosis en bovinos del tr\u00f3pico alto de la zona central cafetera. Prevalencia por examen directo y cultivo de orina","volume":"27","author":"Boh\u00f3rquez","year":"2002","journal-title":"ACOVEZ Colombia."},{"key":"10.1016\/j.mimet.2010.10.021_bb0030","first-page":"52","article-title":"Reproductive failure associated with Leptospira interrogans serovar bratislava infection of swine","volume":"3","author":"Bolin","year":"1996","journal-title":"Vet Diagn Invest."},{"key":"10.1016\/j.mimet.2010.10.021_bb0035","first-page":"20","article-title":"Estandarizaci\u00f3n y validaci\u00f3n de una prueba de PCR para el diagn\u00f3stico precoz de Leptospirosis","volume":"24","author":"C\u00e9spedes","year":"2007","journal-title":"Rev Peru Med Exp Salud P\u00fablica."},{"key":"10.1016\/j.mimet.2010.10.021_bb0040","first-page":"217","article-title":"Leptospirosis. Revisi\u00f3n de tema y experiencia de casos durante una d\u00e9cada en el hospital cl\u00ednico de la Universidad de Chile","volume":"12","author":"Cornejo","year":"2001","journal-title":"Rev. hospital Cl\u00edn. Univ. Chile"},{"key":"10.1016\/j.mimet.2010.10.021_bb0050","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1128\/JCM.40.4.1464-1469.2002","article-title":"Evaluation of eight rapid screening tests for acute leptospirosis in Hawaii","volume":"40","author":"Effler","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0055","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0167-5877(84)90085-0","article-title":"Bovine leptospirosis in the tropics: prevalence, pathogenesis and control","volume":"2","author":"Ellis","year":"1984","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.mimet.2010.10.021_bb0060","series-title":"Leptospira and Leptospirosis","author":"Faine","year":"1999"},{"key":"10.1016\/j.mimet.2010.10.021_bb0065","first-page":"215","article-title":"Epidemiological study of Leptospira hardjo infection in second calf dairy cows","volume":"112","author":"Hathaway","year":"1998","journal-title":"Vet. Rec."},{"key":"10.1016\/j.mimet.2010.10.021_bb0070","unstructured":"ICA Instituto Colombiano Agropecuario., 1999. Informes Anuales de los a\u00f1os 1997, 1998, 1999 \u2014 Divisi\u00f3n de Sanidad Animal, Coordinaci\u00f3n Grupo Diagn\u00f3stico Veterinario. Colombia."},{"key":"10.1016\/j.mimet.2010.10.021_bb0075","series-title":"Appleton and Lange. East Norwalk, Connecticut","first-page":"570","article-title":"Zinsser Microbiology","author":"Jolkik","year":"1988"},{"key":"10.1016\/j.mimet.2010.10.021_bb0080","doi-asserted-by":"crossref","first-page":"278","DOI":"10.4269\/ajtmh.2002.67.278","article-title":"The importance of leptospirosis in southeast Asia","volume":"67","author":"Kantilaras","year":"2002","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.mimet.2010.10.021_bb0085","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/nrmicro2208","article-title":"Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen","volume":"7","author":"Ko","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0090","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0300-8932(97)73190-7","article-title":"M\u00e9todos de investigaci\u00f3n en cardiolog\u00eda cl\u00ednica (IV). Las mediciones cl\u00ednicas en cardiolog\u00eda: validez y errores de medici\u00f3n","volume":"50","author":"Latour","year":"1997","journal-title":"Rev. Esp. Cardiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0095","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1177\/104063870601800216","article-title":"Identification of pathogenic Leptospira strain in tissues of premature foal by use of polymerase chain reaction analysis","volume":"18","author":"Le\u00f3n","year":"2006","journal-title":"J Vet Diagn Invest."},{"key":"10.1016\/j.mimet.2010.10.021_bb0100","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1099\/jmm.0.45860-0","article-title":"Detection of pathogenic leptospires by real-time quantitative PCR","volume":"54","author":"Levett","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0105","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1128\/JCM.43.2.539-545.2005","article-title":"Identification of variable number tandem-repeat loci in Leptospira interrogans sensu stricto","volume":"43","author":"Majed","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0110","first-page":"18","article-title":"Leptospiral antibodies in stray dogs of Moreno, Province of Buenos Aires","volume":"12","author":"Meyers","year":"1998","journal-title":"Rev. Argent. Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0115","series-title":"Centro Panamericano de Zoonosis, OPS\/OMS; (Nota t\u00e9cnica 30)","article-title":"Leptospirosis: manual de m\u00e9todos para el diagn\u00f3stico de laboratorio. Buenos Aires","author":"Myers","year":"1985"},{"key":"10.1016\/j.mimet.2010.10.021_bb0120","first-page":"343","article-title":"Phenotipic and genotipic conservation of ompL1 and ompL41 among Leptospiral isolates of Andaman Island","volume":"122","author":"Natarajaseenivasan","year":"2005","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.mimet.2010.10.021_bb0125","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1590\/S1020-49892004001000005","article-title":"Sistema de aglutinaci\u00f3n con l\u00e1tex para el diagn\u00f3stico r\u00e1pido de la Leptospirosis en Cuba","volume":"16","author":"Obreg\u00f3n","year":"2004","journal-title":"Rev. Panam. Salud P\u00fablica"},{"key":"10.1016\/j.mimet.2010.10.021_bb0130","first-page":"205","article-title":"Leptospirosis en personas de riesgo de quince explotaciones porcinas y de la central de sacrificio de Manizales, Colombia","volume":"35","author":"Orrego","year":"2003","journal-title":"Arch Med Vet."},{"key":"10.1016\/j.mimet.2010.10.021_bb0135","series-title":"Leptospirosis Bovina en el Departamento de C\u00f3rdoba","first-page":"59","author":"Otte","year":"1991"},{"key":"10.1016\/j.mimet.2010.10.021_bb0140","unstructured":"(PAHO) Pan-American Health Organization, 1997. Organization Technical Report. Pan-American Health Organization. Zoonoses cases reported between 1992 and 1996."},{"key":"10.1016\/j.mimet.2010.10.021_bb0145","first-page":"641","article-title":"Detection of IgM specific antibody using indirect immunofluorescent assay for diagnosis of acute leptospirosis","volume":"86","author":"Pradutkanchana","year":"2003","journal-title":"J. Med. Assoc. Thai."},{"key":"10.1016\/j.mimet.2010.10.021_bb0150","first-page":"175","article-title":"Comparison of Leptospiral infection in the horse and donkey","volume":"49","author":"Rahim","year":"2005","journal-title":"Bull Vet Ins Pulaway."},{"key":"10.1016\/j.mimet.2010.10.021_bb0155","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/S0034-5288(18)31076-2","article-title":"Species differentiation of Leptospira interrogans serovars hardjo strain Hardjo bovis from strain Hardjoprajitno by DNA slot blot hybridisation","volume":"49","author":"Ramadass","year":"1990","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.mimet.2010.10.021_bb0160","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1371\/journal.ppat.0030097","article-title":"The OmpA-like protein Loa22 is essential for leptospiral virulence","volume":"3","author":"Ristow","year":"2007","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.mimet.2010.10.021_bb0210","unstructured":"Sambrook, Russell, 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. 3rd Eds. pp 2.344."},{"key":"10.1016\/j.mimet.2010.10.021_bb0170","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0255-0857(21)02650-5","article-title":"Multiplex PCR on leptospiral isolates from Kolenchery, Kerala, I. Indian","volume":"23","author":"Sugathan","year":"2005","journal-title":"J Med Microbiol."},{"key":"10.1016\/j.mimet.2010.10.021_bb0175","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1586\/14737159.8.1.53","article-title":"Proteomics in leptospirosis research: towards molecular diagnostics and vaccine development","volume":"8","author":"Thongboonkerd","year":"2008","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1016\/j.mimet.2010.10.021_bb0180","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1128\/AAC.46.3.848-853.2002","article-title":"Quantitative PCR assay to evaluate ampicillin, ofloxacin, and doxycycline for treatment of experimental leptospirosis","volume":"46","author":"Truccolo","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2010.10.021_bb0185","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1590\/S1020-49892000000100006","article-title":"Descripci\u00f3n de un brote de Leptospirosis en la ciudad de Santa Fe, Argentina, marzo\u2013abril 1998","volume":"7","author":"Vanasco","year":"2000","journal-title":"Rev. Panam. Salud P\u00fablica"},{"key":"10.1016\/j.mimet.2010.10.021_bb0190","first-page":"27","article-title":"Conservation of the S10-spc-alpha locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira","volume":"16","author":"Victoria","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.mimet.2010.10.021_bb0195","first-page":"143","article-title":"Diseases and health damage","volume":"1","author":"(WHO) World Health Organization","year":"1998","journal-title":"Health in America"},{"key":"10.1016\/j.mimet.2010.10.021_bb0200","series-title":"Guidance for diagnosis, surveillance and control","first-page":"57","article-title":"Leptospirosis","year":"2003"},{"key":"10.1016\/j.mimet.2010.10.021_bb0205","series-title":"Unidad de informaci\u00f3n y Vigilancia Epidemiol\u00f3gica Subgerencia de prevenci\u00f3n y control Divisi\u00f3n de Sanidad Animal ICA (Instituto Colombiano Agropecuario)","author":"Z\u00fa\u00f1iga","year":"1996"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701210003635?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701210003635?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:42:11Z","timestamp":1759102931000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701210003635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["S0167701210003635"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2010.10.021","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2011,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A comparison between polymerase chain reaction (PCR) and traditional techniques for the diagnosis of leptospirosis in bovines","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2010.10.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}