{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:57:39Z","timestamp":1792429059748,"version":"4.5.0"},"reference-count":50,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2011,4]]},"DOI":"10.1016\/j.mimet.2010.12.027","type":"journal-article","created":{"date-parts":[[2011,1,22]],"date-time":"2011-01-22T04:34:48Z","timestamp":1295670888000},"page":"16-21","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":91,"title":["Improving qPCR efficiency in environmental samples by selective removal of humic acids with DAX-8"],"prefix":"10.1016","volume":"85","author":[{"given":"A.","family":"Schriewer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Wehlmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Wuertz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2010.12.027_bb0250","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/24.13.2623","article-title":"PicoGreen quantitation of DNA: Effective evaluation of samples pre- or post-PCR","volume":"24","author":"Ahn","year":"1996","journal-title":"Nucleic. Acids. Res."},{"key":"10.1016\/j.mimet.2010.12.027_bb0005","doi-asserted-by":"crossref","first-page":"4463","DOI":"10.1128\/JCM.38.12.4463-4470.2000","article-title":"Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat","volume":"38","author":"Al-Soud","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0010","article-title":"Improved purification and PCR amplification of DNA from environmental samples","volume":"62","author":"Arbeli","year":"2008","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"10.1016\/j.mimet.2010.12.027_bb0015","doi-asserted-by":"crossref","first-page":"6611","DOI":"10.1128\/AEM.70.11.6611-6618.2004","article-title":"Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters","volume":"70","author":"Audemard","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0020","doi-asserted-by":"crossref","first-page":"333","DOI":"10.2144\/05393ST01","article-title":"Kinetics quality assessment for relative quantification by real-time PCR","volume":"39","author":"Bar","year":"2005","journal-title":"Biotechniques"},{"key":"10.1016\/j.mimet.2010.12.027_bb0025","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/jcm.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic-acids","volume":"28","author":"Boom","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0030","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0167-7012(02)00210-5","article-title":"Removal of PCR inhibitors from soil DNA by chemical flocculation","volume":"52","author":"Braid","year":"2003","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mimet.2010.12.027_bb0035","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1128\/AEM.64.2.669-677.1998","article-title":"Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR","volume":"64","author":"Chandler","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0040","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1745-4581.2008.00120.x","article-title":"Enumeration of immunomagnetically captured Escherichia coli in water samples using quantum dot-labeled antibodies","volume":"16","author":"Dudak","year":"2008","journal-title":"J. Rapid Methods Autom. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0045","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.ab.2006.08.022","article-title":"Absolute quantitation of microRNAs with a PCR-based assay","volume":"359","author":"Duncan","year":"2006","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mimet.2010.12.027_bb0050","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0167-7012(03)00005-8","article-title":"An immunomagnetic separation-real-time PCR method for quantification of Cryptosporidium parvum in water samples","volume":"54","author":"Fontaine","year":"2003","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mimet.2010.12.027_bb0055","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1128\/jcm.34.9.2125-2128.1996","article-title":"Substances interfering with direct detection of Mycobacterium tuberculosis in clinical specimens by PCR: effects of bovine serum albumin","volume":"34","author":"Forbes","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0060","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0166-0934(97)00072-4","article-title":"Reactivity and amplification efficiency of the NASBA HIV-1 RNA amplification system with regard to different HIV-1 subtypes","volume":"66","author":"Gobbers","year":"1997","journal-title":"J. Virol. Meth."},{"key":"10.1016\/j.mimet.2010.12.027_bb0065","doi-asserted-by":"crossref","first-page":"5178","DOI":"10.1128\/AEM.69.9.5178-5185.2003","article-title":"Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage","volume":"69","author":"Guy","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0070","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s00436-006-0428-0","article-title":"Real-time PCR for quantifying Haemonchus contortus eggs and potential limiting factors","volume":"101","author":"Harmon","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.mimet.2010.12.027_bb0075","doi-asserted-by":"crossref","first-page":"1916","DOI":"10.1016\/j.bios.2006.08.016","article-title":"Rapid direct determination using combined separation by prepared immunomagnetic and flow cytometry of Flavobacterium psychrophilum","volume":"22","author":"Hibi","year":"2007","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.mimet.2010.12.027_bb0080","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0167-7012(03)00006-X","article-title":"A quantitative analysis of DNA extraction and purification from compost","volume":"54","author":"Howeler","year":"2003","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mimet.2010.12.027_bb0085","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1128\/AEM.71.3.1135-1141.2005","article-title":"Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors","volume":"71","author":"Jiang","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0090","doi-asserted-by":"crossref","first-page":"3849","DOI":"10.1128\/aem.61.11.3849-3855.1995","article-title":"Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples","volume":"61","author":"Johnson","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0095","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1128\/aem.62.3.1102-1106.1996","article-title":"Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein","volume":"62","author":"Kreader","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0100","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1023\/A:1005297006179","article-title":"Detection of infectious Cryptosporidium parvum oocysts in environmental water samples using an integrated cell culture-PCR (CC-PCR) system","volume":"123","author":"Lechevallier","year":"2000","journal-title":"Water Air Soil Pollut."},{"key":"10.1016\/j.mimet.2010.12.027_bb0105","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0006-291X(02)00478-3","article-title":"Validation of a quantitative method for real time PCR kinetics","volume":"294","author":"Liu","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.mimet.2010.12.027_bb0110","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1021\/es015777m","article-title":"PCR detection of specific pathogens in water: a risk-based analysis","volume":"36","author":"Loge","year":"2002","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0115","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1017\/S0950268801006276","article-title":"PCR-IMS detection and molecular typing of Cryptosporidium parvum recovered from a recreational river source and an associated mussel (Mytilus edulis) bed in Northern Ireland","volume":"127","author":"Lowery","year":"2001","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.mimet.2010.12.027_bb0120","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0167-7012(96)00961-X","article-title":"A method for the detection and quantitation of PCR template in environmental samples by high performance liquid chromatography","volume":"28","author":"Marlowe","year":"1997","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mimet.2010.12.027_bb0125","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.chroma.2006.08.086","article-title":"Isolation and seasonal effects on characteristics of fulvic acid isolated from an Australian floodplain river and billabong","volume":"1153","author":"McDonald","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.mimet.2010.12.027_bb0130","doi-asserted-by":"crossref","first-page":"88","DOI":"10.2144\/00291st03","article-title":"Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR","volume":"29","author":"Monpoeho","year":"2000","journal-title":"Biotechniques"},{"key":"10.1016\/j.mimet.2010.12.027_bb0135","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.1093\/nar\/26.13.3309","article-title":"Efficient removal of PCR inhibitors using agarose-embedded DNA preparations","volume":"26","author":"Moreira","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2010.12.027_bb0140","doi-asserted-by":"crossref","first-page":"246","DOI":"10.2144\/04372RR01","article-title":"Potential influence of the first PCR cycles in real-time comparative gene quantifications","volume":"37","author":"Nogva","year":"2004","journal-title":"Biotechniques"},{"key":"10.1016\/j.mimet.2010.12.027_bb0145","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0003-2670(02)00931-5","article-title":"Sorption of aquatic humic matter by DAX-8 and XAD-8 resins \u2014 comparative study using pyrolysis gas chromatography","volume":"471","author":"Peuravuori","year":"2002","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.mimet.2010.12.027_bb0150","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.talanta.2004.06.042","article-title":"Comparative study for separation of aquatic humic-type organic constituents by DAX-8, PVP and DEAE sorbing solids and tangential ultrafiltration: elemental composition, size-exclusion chromatography, UV\u2013vis and FT-IR","volume":"65","author":"Peuravuori","year":"2005","journal-title":"Talanta"},{"key":"10.1016\/j.mimet.2010.12.027_bb0155","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2010.12.027_bb0160","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1039\/b716138g","article-title":"Quantitative PCR analysis of fungi and bacteria in building materials and comparison to culture-based analysis","volume":"10","author":"Pietarinen","year":"2008","journal-title":"J. Environ. Monit."},{"key":"10.1016\/j.mimet.2010.12.027_bb0165","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0167-7012(02)00111-2","article-title":"Evaluation of procedures for reliable PCR detection of Ralstonia solanacearum in common natural substrates","volume":"51","author":"Poussier","year":"2002","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mimet.2010.12.027_bb0170","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1128\/CVI.00270-07","article-title":"Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as an inhibitor in real-time PCR for serum samples from patients with brucellosis","volume":"15","author":"Queipo-Ortuno","year":"2008","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0175","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1016\/j.watres.2006.12.034","article-title":"Validation of hollow fiber ultrafiltration and real-time PCR using bacteriophage PP7 as surrogate for the quantification of viruses from water samples","volume":"41","author":"Rajal","year":"2007","journal-title":"Water Res."},{"key":"10.1016\/j.mimet.2010.12.027_bb0180","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/S0304-3940(02)01423-4","article-title":"Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data","volume":"339","author":"Ramakers","year":"2003","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.mimet.2010.12.027_bb0185","doi-asserted-by":"crossref","first-page":"5610","DOI":"10.1128\/AEM.00364-06","article-title":"Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions","volume":"72","author":"Reischer","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0190","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1128\/aem.63.1.106-114.1997","article-title":"Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water","volume":"63","author":"Rochelle","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0195","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0929-1393(01)00149-4","article-title":"Extraction and purification of microbial DNA from soil and sediment samples","volume":"18","author":"Roose-Amsaleg","year":"2001","journal-title":"Appl. Soil Ecol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0200","doi-asserted-by":"crossref","first-page":"5802","DOI":"10.1128\/AEM.00635-10","article-title":"Bacteroidales as a predictor of pathogens in surface waters of the central California coast","volume":"76","author":"Schriewer","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0205","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.bbrc.2004.08.168","article-title":"Effect of DNA damage on PCR amplification efficiency with the relative threshold cycle method","volume":"323","author":"Sikorsky","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.mimet.2010.12.027_bb0210","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s002530051057","article-title":"Parameters affecting polymerase chain reaction detection of waterborne Cryptosporidium parvum oocysts","volume":"48","author":"Sluter","year":"1997","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0215","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1023\/A:1021319421153","article-title":"Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency","volume":"24","author":"Tichopad","year":"2002","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.mimet.2010.12.027_bb0220","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1128\/aem.57.4.1070-1074.1991","article-title":"Rapid method for direct extraction of DNA from soil and sediments","volume":"57","author":"Tsai","year":"1991","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0225","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1128\/aem.58.2.754-757.1992","article-title":"Detection of low numbers of bacterial-cells in soils and sediments by polymerase chain-reaction","volume":"58","author":"Tsai","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0230","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s00253-007-1196-1","article-title":"Improving PCR and qPCR detection of hydrogenase A (hydA) associated with Clostridia in pure cultures and environmental sludges using bovine serum albumin","volume":"77","author":"Wang","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0235","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1128\/jcm.33.10.2643-2646.1995","article-title":"Inhibition of PCR by aqueous and vitreous fluids","volume":"33","author":"Wiedbrauk","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0240","doi-asserted-by":"crossref","first-page":"3741","DOI":"10.1128\/aem.63.10.3741-3751.1997","article-title":"Inhibition and facilitation of nucleic acid amplification","volume":"63","author":"Wilson","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2010.12.027_bb0245","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1128\/JCM.42.1.408-411.2004","article-title":"Impact of DNA polymerases and their buffer systems on quantitative real-time PCR","volume":"42","author":"Wolffs","year":"2004","journal-title":"J. Clin. Microbiol."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701211000030?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701211000030?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:42:32Z","timestamp":1759102952000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701211000030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["S0167701211000030"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2010.12.027","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2011,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Improving qPCR efficiency in environmental samples by selective removal of humic acids with DAX-8","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2010.12.027","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2011 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}