{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:02:08Z","timestamp":1761580928643,"version":"3.44.0"},"reference-count":23,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2012,1]]},"DOI":"10.1016\/j.mimet.2011.10.017","type":"journal-article","created":{"date-parts":[[2011,10,28]],"date-time":"2011-10-28T20:03:23Z","timestamp":1319832203000},"page":"98-102","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Rapid detection by multiplex PCR of Genomic Islands, prophages and Integrative Conjugative Elements in V. cholerae 7th pandemic variants"],"prefix":"10.1016","volume":"88","author":[{"given":"Matteo","family":"Spagnoletti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Ceccarelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro M.","family":"Colombo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2011.10.017_bb0120","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.3201\/eid1011.040682","article-title":"Cholera in Mozambique, variant of Vibrio cholerae","volume":"10","author":"Ansaruzzaman","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2011.10.017_bb0010","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1574-6968.2006.00518.x","article-title":"Molecular characterization of ICEVchVie0 and its disappearance in Vibrio cholerae O1 strains isolated in 2003 in Vietnam","volume":"266","author":"Bani","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2011.10.017_bb0020","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.ijmm.2010.11.005","article-title":"ICEVchInd5 is prevalent in epidemic Vibrio cholerae O1 El Tor strains isolated in India","volume":"301","author":"Ceccarelli","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0025","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/S1473-3099(11)70078-0","article-title":"Origin of Vibrio cholerae in Haiti","volume":"11","author":"Ceccarelli","year":"2011","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.mimet.2011.10.017_bb0125","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/1471-2180-11-130","article-title":"New V. cholerae atypical El Tor variant emerged during the 2006 epidemic outbreak in Angola","volume":"11","author":"Ceccarelli","year":"2011","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0030","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1128\/AEM.66.9.4022-4028.2000","article-title":"Virulence genes in environmental strains of Vibrio cholerae","volume":"66","author":"Chakraborty","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0035","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1056\/NEJMoa1012928","article-title":"The origin of the Haitian cholera outbreak strain","volume":"364","author":"Chin","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.mimet.2011.10.017_bb0040","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.mib.2010.08.007","article-title":"Genomic evolution of Vibrio cholerae","volume":"13","author":"Cho","year":"2010","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0045","doi-asserted-by":"crossref","first-page":"15442","DOI":"10.1073\/pnas.0907787106","article-title":"Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae","volume":"106","author":"Chun","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mimet.2011.10.017_bb0050","doi-asserted-by":"crossref","first-page":"5151","DOI":"10.1073\/pnas.0700365104","article-title":"Genomic analysis of the Mozambique strain of Vibrio cholerae O1 reveals the origin of El Tor strains carrying classical CTX prophage","volume":"104","author":"Faruque","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mimet.2011.10.017_bb0055","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1128\/JCM.38.11.4249-4253.2000","article-title":"Emergence of Vibrio cholerae O1 biotype El Tor serotype Inaba from the prevailing O1 Ogawa serotype strains in India","volume":"38","author":"Garg","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0060","doi-asserted-by":"crossref","first-page":"3524","DOI":"10.1128\/JB.00040-10","article-title":"Genome sequence of hybrid V. cholerae O1 MJ-1236, B-33 and CIRS101 and comparative genomics with V. cholerae","volume":"192","author":"Grim","year":"2010","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0065","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1038\/nature09469","article-title":"Satellite phage TLCphi enables toxigenic conversion by CTX phage through dif site alteration","volume":"467","author":"Hassan","year":"2010","journal-title":"Nature"},{"year":"2010","series-title":"Java web tools for PCR, in silico PCR, and oligonucleotide assembly and analyses","author":"Kalendar","key":"10.1016\/j.mimet.2011.10.017_bb0130"},{"key":"10.1016\/j.mimet.2011.10.017_bb0075","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1007\/s12275-009-0292-6","article-title":"Classification of hybrid and altered Vibrio cholerae strains by CTX prophage and RS1 element structure","volume":"47","author":"Lee","year":"2009","journal-title":"J. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0080","doi-asserted-by":"crossref","first-page":"4053","DOI":"10.1099\/mic.0.27172-0","article-title":"The Vibrio seventh pandemic island-II is a 26.9 kb genomic island present in Vibrio cholerae El Tor and O139 serogroup isolates that shows homology to a 43.4 kb genomic island in V. vulnificus","volume":"150","author":"O'Shea","year":"2004","journal-title":"Microbiology"},{"key":"10.1016\/j.mimet.2011.10.017_bb0085","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S1286-4579(03)00035-2","article-title":"Vibrio cholerae O139 Bengal: odyssey of a fortuitous variant","volume":"5","author":"Ramamurthy","year":"2003","journal-title":"Microbes Infect."},{"key":"10.1016\/j.mimet.2011.10.017_bb0090","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1046\/j.1365-2958.1998.00889.x","article-title":"Replication and integration of a Vibrio cholerae cryptic plasmid linked to the CTX prophage","volume":"28","author":"Rubin","year":"1998","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0095","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.tim.2009.10.003","article-title":"Evolution of new variants of Vibrio cholerae O1","volume":"18","author":"Safa","year":"2010","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0100","doi-asserted-by":"crossref","first-page":"352","DOI":"10.3201\/eid1502.080943","article-title":"Outbreaks caused by new variants of Vibrio cholerae O1 El Tor, India","volume":"15","author":"Taneja","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2011.10.017_bb0105","first-page":"130","article-title":"Discovery of novel Vibrio cholerae VSP-II genomic islands using comparative genomic analysis","volume":"308","author":"Taviani","year":"2010","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2011.10.017_bb0115","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/nrmicro2382","article-title":"Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow","volume":"8","author":"Wozniak","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.mimet.2011.10.017_bb0110","doi-asserted-by":"crossref","first-page":"e1000786","DOI":"10.1371\/journal.pgen.1000786","article-title":"Comparative ICE genomics: insights into the evolution of the SXT\/R391 family of ICEs","volume":"5","author":"Wozniak","year":"2009","journal-title":"PLoS Genet."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770121100371X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770121100371X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:45:28Z","timestamp":1759103128000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016770121100371X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["S016770121100371X"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2011.10.017","relation":{},"ISSN":["0167-7012"],"issn-type":[{"type":"print","value":"0167-7012"}],"subject":[],"published":{"date-parts":[[2012,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid detection by multiplex PCR of Genomic Islands, prophages and Integrative Conjugative Elements in V. cholerae 7th pandemic variants","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2011.10.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2011 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}