{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T09:41:55Z","timestamp":1780652515732,"version":"3.54.1"},"reference-count":31,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"German Research Foundation (DFG)","award":["BE 2157\/3-1"],"award-info":[{"award-number":["BE 2157\/3-1"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1016\/j.mimet.2013.01.009","type":"journal-article","created":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T18:19:06Z","timestamp":1359137946000},"page":"264-269","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule"],"prefix":"10.1016","volume":"92","author":[{"given":"M.P.","family":"Owen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Schauwers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.E.","family":"Hugh-Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.A.","family":"Kiernan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.C.B.","family":"Turnbull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Beyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2013.01.009_bb0005","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1083\/jcb.4.4.433","article-title":"Metachromasy: an experimental and theoretical reevaluation","volume":"4","author":"Bergeron","year":"1958","journal-title":"J. Biophys. Biochem. Cytol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0015","first-page":"359","article-title":"Sheep","volume":"No. 58","author":"Eamens","year":"1981"},{"key":"10.1016\/j.mimet.2013.01.009_bb0020","first-page":"42","article-title":"Encapsulation of Bacillus anthracis spores and spore identification","volume":"No. 87S","author":"Ezzell","year":"1996"},{"key":"10.1016\/j.mimet.2013.01.009_bb0025","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/j.1751-0813.1996.tb15453.x","article-title":"Anthrax in cattle in southern Western Australia","volume":"74","author":"Forshaw","year":"1996","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.mimet.2013.01.009_bb0030","series-title":"The Sigma-Aldrich Handbook of Stains, Dyes and Indicators","author":"Green","year":"1990"},{"key":"10.1016\/j.mimet.2013.01.009_bb0035","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3109\/10520292709115643","article-title":"Investigations of thiazin dyes as biological stains. I. The staining properties of thionin and its derivatives as compared with their chemical formulae","volume":"2","author":"Haynes","year":"1927","journal-title":"Stain. Technol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0040","first-page":"55","article-title":"Zur Milzbrandinfektion","volume":"40","author":"Heim","year":"1901","journal-title":"Arch. Hyg. (Berlin)"},{"key":"10.1016\/j.mimet.2013.01.009_bb0045","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3109\/10520292609115611","article-title":"The oxidation products of methylene blue","volume":"1","author":"Holmes","year":"1926","journal-title":"Stain. Technol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0050","series-title":"Histochemistry","author":"Horobin","year":"1982"},{"key":"10.1016\/j.mimet.2013.01.009_bb0055","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3109\/10520295.2010.515491","article-title":"How Romanowski stains work and why they remain valuable \u2014 including a proposed universal Romanowski staining mechanism and a rational troubleshooting scheme","volume":"86","author":"Horobin","year":"2011","journal-title":"Biotech. Histochem."},{"key":"10.1016\/j.mimet.2013.01.009_bb0010","first-page":"707","article-title":"ICSH reference method for staining of blood and bone marrow films by azure B and eosin Y (Romanowsky stain)","volume":"57","author":"International Committee for Standardization in Haematology","year":"1984","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0065","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1002\/cber.19060390246","article-title":"Ueber Methylen-azur","volume":"39","author":"Kehrmann","year":"1906","journal-title":"Ber. Dtsch. Chem. Ges."},{"key":"10.1016\/j.mimet.2013.01.009_bb0070","first-page":"283","article-title":"Histological and Histochemical Methods: Theory and Practice","author":"Kiernan","year":"2008"},{"key":"10.1016\/j.mimet.2013.01.009_bb0075","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3109\/10520295.2010.515492","article-title":"Romanowsky staining in cytopathology: history, advantages and limitations","volume":"86","author":"Krafts","year":"2011","journal-title":"Biotech. Histochem."},{"key":"10.1016\/j.mimet.2013.01.009_bb0080","series-title":"H. J. Conn's Biological Stains","first-page":"425","author":"Lillie","year":"1977"},{"key":"10.1016\/j.mimet.2013.01.009_bb0085","series-title":"Histopathologic Technic and Practical Histochemistry","first-page":"133","author":"Lillie","year":"1976"},{"key":"10.1016\/j.mimet.2013.01.009_bb0090","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/BF01003831","article-title":"The composition of stains produced by the oxidation of methylene blue","volume":"8","author":"Marshall","year":"1976","journal-title":"Histochem. J."},{"key":"10.1016\/j.mimet.2013.01.009_bb0095","first-page":"153","article-title":"Methylene blue-azure B-eosin as a substitute for May\u2013Grunwald\u2013Giemsa and Jenner\u2013Giemsa stains","volume":"79","author":"Marshall","year":"1977","journal-title":"Microsc. Acta"},{"key":"10.1016\/j.mimet.2013.01.009_bb0100","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0368-1742(03)80006-1","article-title":"A peculiar staining reaction of the blood of animals dead of anthrax","volume":"16","author":"M'Fadyean","year":"1903","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0105","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/S0368-1742(03)80093-0","article-title":"A further note with regard to the staining reaction of anthrax blood with methylene blue","volume":"16","author":"M'Fadyean","year":"1903","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0110","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1146\/annurev.micro.55.1.647","article-title":"Anthrax","volume":"55","author":"Mock","year":"2001","journal-title":"Annu. Rev. Microbiol."},{"issue":"79","key":"10.1016\/j.mimet.2013.01.009_bb0115","first-page":"107","article-title":"The M'Fadyean reaction: a stain for anthrax bacilli","volume":"2004","author":"Owen","year":"2004","journal-title":"Biotech. Histochem."},{"key":"10.1016\/j.mimet.2013.01.009_bb0120","doi-asserted-by":"crossref","first-page":"65","DOI":"10.3109\/10520298809107162","article-title":"Note from the biological stain commission: revision of azure b assay protocol","volume":"63","author":"Schenk","year":"1988","journal-title":"Stain. Technol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0125","doi-asserted-by":"crossref","first-page":"2325.e7","DOI":"10.1016\/j.neurobiolaging.2010.12.012","article-title":"Lest we forget you \u2014 methylene blue","volume":"32","author":"Schirmer","year":"2011","journal-title":"Neurobiol. Aging"},{"key":"10.1016\/j.mimet.2013.01.009_bb0130","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1177\/8.4.248","article-title":"Chromatographic separation and isolation of metachromatic thiazine dyes","volume":"8","author":"Taylor","year":"1960","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.mimet.2013.01.009_bb0135","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3109\/10520296109113247","article-title":"The influence of molecular structure of thiazine and oxazine dyes on their metachromatic properties","volume":"36","author":"Taylor","year":"1961","journal-title":"Stain. Technol."},{"key":"10.1016\/j.mimet.2013.01.009_bb0140","series-title":"The Elements of Pathological Histology with Special Reference to Practical Methods","author":"Weichselbaum","year":"1895"},{"key":"10.1016\/j.mimet.2013.01.009_bb0145","series-title":"Anthrax in Humans and Animals","author":"WHO","year":"2008"},{"key":"10.1016\/j.mimet.2013.01.009_bb0150","series-title":"Conn's Biological Stains","first-page":"303","article-title":"Romanowsky\u2013Giemsa stains","author":"Wittekind","year":"2002"},{"key":"10.1016\/j.mimet.2013.01.009_bb0155","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/BF01680459","article-title":"On the nature of Romanowsky\u2013Giemsa staining and the Romanowsky\u2013Giemsa effect. II. A revised Romanowsky\u2013Giemsa staining procedure","volume":"19","author":"Wittekind","year":"1987","journal-title":"Histochem. J."},{"key":"10.1016\/j.mimet.2013.01.009_bb0160","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF00995934","article-title":"Kann Azur B-Eosin die May-Gr\u00fcnwald-Giemsa F\u00e4rbung ersetzen?","volume":"32","author":"Wittekind","year":"1976","journal-title":"Blut"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701213000134?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701213000134?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:46:57Z","timestamp":1759103217000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701213000134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["S0167701213000134"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2013.01.009","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2013,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A simple, reliable M'Fadyean stain for visualizing the Bacillus anthracis capsule","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2013.01.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}