{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:28:40Z","timestamp":1791019720340,"version":"4.1.0"},"reference-count":54,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.mimet.2013.05.011","type":"journal-article","created":{"date-parts":[[2013,6,6]],"date-time":"2013-06-06T12:57:32Z","timestamp":1370523452000},"page":"280-289","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of the viability of Cryptosporidium parvum oocysts with the induction ratio of hsp70 mRNA production in manure"],"prefix":"10.1016","volume":"94","author":[{"given":"Gabriela","family":"Garc\u00e9s-Sanchez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Wilderer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Horn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Charles","family":"Munch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lebuhn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2013.05.011_bb0005","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1021\/ac001293h","article-title":"Detection of viable oocysts in Cryptosporidium parvum following nucleic acid sequence based amplification","volume":"73","author":"Baeumner","year":"2001","journal-title":"Anal. Chem."},{"key":"10.1016\/j.mimet.2013.05.011_bb0010","series-title":"Investigations on Microbial Indicators and\/or Test Organisms in Supervision of Hygienic Safety in Co-digestion of Animal Slurry, Biowaste and\/or Animal Byproducts","first-page":"283","author":"B\u00f6hm","year":"2004"},{"issue":"10","key":"10.1016\/j.mimet.2013.05.011_bb9000","doi-asserted-by":"crossref","first-page":"2592","DOI":"10.1128\/jcm.33.10.2592-2595.1995","article-title":"Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces","volume":"33","author":"Bukhari","year":"1995","journal-title":"J. Clinical Microbiol."},{"issue":"11","key":"10.1016\/j.mimet.2013.05.011_bb7500","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/aem.58.11.3488-3493.1992","article-title":"Viability of Cryptosporidium parvum Oocysts: Correlation of In Vitro Excystation with Inclusion or Exclusion of Fluorogenic Vital Dyes","volume":"58","author":"Campbell","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0015","doi-asserted-by":"crossref","first-page":"3528","DOI":"10.1016\/j.watres.2008.05.001","article-title":"Contribution of treated wastewater to the contamination of recreational river areas with Cryptosporidium spp. and Giardia duodenalis","volume":"42","author":"Castro-Hermida","year":"2008","journal-title":"Water Res."},{"key":"10.1016\/j.mimet.2013.05.011_bb0020","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/0012-1606(92)90215-3","article-title":"Gene expression in the Caenorhabditis elegans dauer larva: developmental regulation of Hsp90 and other genes","volume":"151","author":"Dalley","year":"1992","journal-title":"Dev. Biol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0030","series-title":"Arbeitsblatt W 101. Deutsche Verein des Gas- und Wasserfaches (DVGW) (German Association of Gas and Water)","article-title":"Richtlinie f\u00fcr Trinkwasserschutzgebiete; I. Teil: Schutzgebiete fur Grundwasser","author":"DVGW","year":"1995"},{"issue":"8","key":"10.1016\/j.mimet.2013.05.011_bb0035","doi-asserted-by":"crossref","first-page":"253","DOI":"10.2166\/wst.2006.256","article-title":"Mesophilic\u2013thermophilic\u2013mesophilic anaerobic digestion of semi-liquid dairy cattle manure","volume":"53","author":"Effenberger","year":"2006","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0045","series-title":"Detecting and Mitigating the Environmental Impact of Fecal Pathogens Originating from Confined Animal Feeding Operations: Review. U.S. EPA\/600\/R-06\/021","author":"EPA","year":"2005"},{"key":"10.1016\/j.mimet.2013.05.011_bb0040","first-page":"1","volume":"300","author":"European Commission","year":"2009","journal-title":"Off. J. Eur. Union"},{"key":"10.1016\/j.mimet.2013.05.011_bb0060","series-title":"Livestock's Long Shadow \u2014 Environmental Issues and Options","author":"FAO","year":"2006"},{"issue":"4","key":"10.1016\/j.mimet.2013.05.011_bb0050","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1128\/aem.62.4.1431-1433.1996","article-title":"Effects of low temperatures on viability of Cryptosporidium parvum oocysts","volume":"62","author":"Fayer","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0055","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetpar.2004.09.004","article-title":"Cryptosporidium: a water-borne zoonotic parasite","volume":"126","author":"Fayer","year":"2004","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0065","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.physiol.61.1.243","article-title":"Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology","volume":"61","author":"Feder","year":"1999","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0070","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0378-1097(03)00538-X","article-title":"Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated Cryptosporidium parvum oocysts","volume":"226","author":"Fontaine","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"issue":"8","key":"10.1016\/j.mimet.2013.05.011_bb0075","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2166\/wst.2006.250","article-title":"Quantification of Cryptosporidium parvum in anaerobic digesters treating manure by (reverse-transcription) quantitative real-time PCR, infectivity and excystation tests","volume":"53","author":"Garc\u00e9s","year":"2006","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0080","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1016\/j.watres.2009.03.019","article-title":"Evaluation of two methods for quantification of hsp70 mRNA from the waterborne pathogen Cryptosporidium parvum by reverse transcription real-time PCR in environmental samples","volume":"43","author":"Garc\u00e9s-Sanchez","year":"2009","journal-title":"Water Res."},{"key":"10.1016\/j.mimet.2013.05.011_bb0085","doi-asserted-by":"crossref","first-page":"42","DOI":"10.2134\/jeq2005.0042a","article-title":"Sources of pathogenic microorganisms and their fate during land application of wastes","volume":"34","author":"Gerba","year":"2005","journal-title":"J. Environ. Qual."},{"issue":"9","key":"10.1016\/j.mimet.2013.05.011_bb0090","doi-asserted-by":"crossref","first-page":"5178","DOI":"10.1128\/AEM.69.9.5178-5185.2003","article-title":"Real-time PCR for the quantification of Giardia and Cryptosporidium in environmental water samples and sewage","volume":"69","author":"Guy","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"issue":"7","key":"10.1016\/j.mimet.2013.05.011_bb0095","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1046\/j.1462-2920.2003.00442.x","article-title":"An immunomagnetic separation-reverse transcription polymerase chain reaction (IMS-RT-PCR) test for sensitive and rapid detection of viable waterborne Cryptosporidium parvum","volume":"5","author":"Hallier-Soulier","year":"2003","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0100","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/j.watres.2005.11.047","article-title":"Changes of physical and biochemical properties of Cryptosporidium oocysts with various storage conditions","volume":"40","author":"Inoue","year":"2006","journal-title":"Water Res."},{"issue":"12","key":"10.1016\/j.mimet.2013.05.011_bb0105","doi-asserted-by":"crossref","first-page":"4060","DOI":"10.1128\/JCM.00807-09","article-title":"Oligonucleotide\u2013gold nanoparticle networks for detection of Cryptosporidium parvum heat shock protein 70 mRNA","volume":"47","author":"Javier","year":"2009","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.mimet.2013.05.011_bb0115","doi-asserted-by":"crossref","first-page":"3844","DOI":"10.1128\/aem.63.10.3844-3850.1997","article-title":"Assessment of a DYE permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts","volume":"63","author":"Jenkins","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0120","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0167-7012(00)00198-6","article-title":"Estimating viability of Cryptosporidium parvum oocysts using reverse transcriptase-polymerase chain reaction (RT-PCR) directed at mRNA encoding amyloglucosidase","volume":"43","author":"Jenkins","year":"2000","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mimet.2013.05.011_bb0125","first-page":"1","article-title":"Comparison of tests for viable and infectious Cryptosporidium parvum oocysts","volume":"89","author":"Jenkins","year":"2003","journal-title":"Parasitol. Res."},{"issue":"3","key":"10.1016\/j.mimet.2013.05.011_bb0130","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1128\/AEM.71.3.1135-1141.2005","article-title":"Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors","volume":"71","author":"Jiang","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"issue":"7","key":"10.1016\/j.mimet.2013.05.011_bb0135","doi-asserted-by":"crossref","first-page":"2820","DOI":"10.1128\/AEM.65.7.2820-2826.1999","article-title":"Method for detection and enumeration of C. parvum oocysts in feces, manures and soils","volume":"65","author":"Kuczynska","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.mimet.2013.05.011_bb0140","doi-asserted-by":"crossref","first-page":"97","DOI":"10.2166\/wst.2003.0230","article-title":"Using quantitative real-time PCR to determine the hygienic status of cattle manure","volume":"48","author":"Lebuhn","year":"2003","journal-title":"Water Sci. Technol."},{"issue":"1","key":"10.1016\/j.mimet.2013.05.011_bb0145","doi-asserted-by":"crossref","first-page":"263","DOI":"10.2166\/wst.2004.0065","article-title":"Evaluating real-time PCR for the quantification of distinct pathogens and indicator organisms in environmental samples","volume":"50","author":"Lebuhn","year":"2004","journal-title":"Water Sci. Technol."},{"issue":"1\u20132","key":"10.1016\/j.mimet.2013.05.011_bb0150","doi-asserted-by":"crossref","first-page":"93","DOI":"10.2166\/wst.2005.0503","article-title":"Hygienization by anaerobic digestion: comparison between evaluation by cultivation and quantitative real-time PCR","volume":"52","author":"Lebuhn","year":"2005","journal-title":"Water Sci. Technol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0155","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/s00436-007-0772-8","article-title":"CP2 gene as a useful viability marker for Cryptosporidium parvum","volume":"102","author":"Lee","year":"2008","journal-title":"Parasitol. Res."},{"issue":"18","key":"10.1016\/j.mimet.2013.05.011_bb0160","doi-asserted-by":"crossref","first-page":"6476","DOI":"10.1128\/AEM.00677-11","article-title":"Detection of viable Cryptosporidium parvum in soil by reverse transcription\u2013real-time PCR targeting hsp70 mRNA","volume":"77","author":"Liang","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0170","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1146\/annurev.bi.55.070186.005443","article-title":"The heat-shock response","volume":"55","author":"Lindquist","year":"1986","journal-title":"Ann. Rev. Biochem."},{"key":"10.1016\/j.mimet.2013.05.011_bb0165","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1146\/annurev.ge.22.120188.003215","article-title":"The heat-shock proteins","volume":"22","author":"Lindquist","year":"1988","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.mimet.2013.05.011_bb0180","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1007\/BF01923463","article-title":"Hsp70 in parasites: as an inducible protective protein and as antigen","volume":"50","author":"Maresca","year":"1994","journal-title":"Birkh\u00e4user Verlag Basel, Experientia"},{"key":"10.1016\/j.mimet.2013.05.011_bb0175","doi-asserted-by":"crossref","first-page":"38","DOI":"10.2174\/1874940201003010038","article-title":"Assessing PCR inhibition from humic substances","volume":"3","author":"Matheson","year":"2010","journal-title":"The Open Enzym. Inhib.J."},{"issue":"1","key":"10.1016\/j.mimet.2013.05.011_bb0185","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s00436-006-0437-z","article-title":"Development and validation of a cell culture based assay for in vitro assessment of anticryptosporidial compounds","volume":"101","author":"Najdrowski","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.mimet.2013.05.011_bb0190","first-page":"1","article-title":"Characterisation and expression of an Hsp70 gene from Parastrongyloides trichosuri","volume":"xx","author":"Newton-Howes","year":"2006","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0195","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1091\/mbc.1.1.135","article-title":"Regulation of hsp70 synthesis by messenger RNA degradation","volume":"1","author":"Petersen","year":"1989","journal-title":"Cell. Regul."},{"key":"10.1016\/j.mimet.2013.05.011_bb0200","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0166-6851(92)90022-C","article-title":"Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase","volume":"53","author":"Requena","year":"1992","journal-title":"Mol. Biochem. Parasit."},{"issue":"8","key":"10.1016\/j.mimet.2013.05.011_bb0205","doi-asserted-by":"crossref","first-page":"3809","DOI":"10.1128\/AEM.68.8.3809-3817.2002","article-title":"Comparison of in vitro cell culture and mouse assay for measurement of infectivity in Cryptosporidium parvum","volume":"68","author":"Rochelle","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0210","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2005.03.007","article-title":"Microbial gene expression in soil: methods, applications and challenges","volume":"63","author":"Saleh-Lakha","year":"2005","journal-title":"J. Microbiol. Methods"},{"issue":"11","key":"10.1016\/j.mimet.2013.05.011_bb0220","doi-asserted-by":"crossref","first-page":"6793","DOI":"10.1128\/AEM.71.11.6793-6798.2005","article-title":"Detection of infectious Cryptosporidium oocysts by cell culture immunofluorescence assay: applicability to environmental samples","volume":"71","author":"Schets","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.mimet.2013.05.011_bb0225","doi-asserted-by":"crossref","first-page":"242","DOI":"10.3855\/jidc.360","article-title":"Cryptosporidiosis in developing countries","volume":"1","author":"Snelling","year":"2007","journal-title":"J. Infect. Dev. Countries"},{"key":"10.1016\/j.mimet.2013.05.011_bb0230","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1139\/o83-064","article-title":"Alterations in the pattern of gene expression following heat shock in the nematode Caenorhabditis elegans","volume":"61","author":"Snutch","year":"1983","journal-title":"Can. J. Biochem. Cell Biol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0235","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0378-1119(88)90339-3","article-title":"The Caenorhabditis elegans hsp70 gene family: a molecular genetic characterization","volume":"64","author":"Snutch","year":"1988","journal-title":"Gene"},{"issue":"3","key":"10.1016\/j.mimet.2013.05.011_bb0250","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1046\/j.1365-2435.2002.00639.x","article-title":"Decreased heat-shock resistance and down-regulation of Hsp70 expression with increasing age in adult Drosophila melanogaster","volume":"16","author":"S\u00f8rensen","year":"2002","journal-title":"Funct. Ecol."},{"issue":"9","key":"10.1016\/j.mimet.2013.05.011_bb0240","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1128\/aem.62.9.3385-3390.1996","article-title":"Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR","volume":"62","author":"Stinear","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"issue":"9","key":"10.1016\/j.mimet.2013.05.011_bb0255","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1099\/jmm.0.46678-0","article-title":"Real-time PCR detection and speciation of Cryptosporidium infection using Scorpion probes","volume":"55","author":"Stroup","year":"2006","journal-title":"J. Med. Microbiol."},{"issue":"6","key":"10.1016\/j.mimet.2013.05.011_bb0245","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1128\/AEM.68.6.2991-2996.2002","article-title":"Immunomagnetic separation (IMS)-fluorescent antibody detection and IMS-PCR detection of seeded Cryptosporidium parvum oocysts in natural waters and their limitations","volume":"68","author":"Sturbaum","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2013.05.011_bb0280","series-title":"The Nematode Caenorhabditis elegans","first-page":"587","article-title":"Methods","author":"Sulston","year":"1988"},{"key":"10.1016\/j.mimet.2013.05.011_bb0265","series-title":"World Health Organization, WHO\/HSE\/WSH\/09.04","article-title":"Risk assessment of Cryptosporidium in drinking-water","author":"WHO","year":"2009"},{"key":"10.1016\/j.mimet.2013.05.011_bb0270","series-title":"World Health Organization","isbn-type":"print","article-title":"Guidelines for Drinking-water Quality Fourth Edition","author":"WHO","year":"2011","ISBN":"https:\/\/id.crossref.org\/isbn\/9789241548151"},{"issue":"4","key":"10.1016\/j.mimet.2013.05.011_bb0275","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1128\/AEM.65.4.1584-1588.1999","article-title":"\u03b2-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability","volume":"65","author":"Widmer","year":"1999","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701213001620?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701213001620?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:48:21Z","timestamp":1759103301000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701213001620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":54,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["S0167701213001620"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2013.05.011","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assessment of the viability of Cryptosporidium parvum oocysts with the induction ratio of hsp70 mRNA production in manure","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2013.05.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}