{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:37:49Z","timestamp":1792784269274,"version":"4.5.0"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Oceans and Human Health Initiative"},{"name":"National Marine Fisheries Service Marine Mammal Health and Stranding Response Program"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2014,5]]},"DOI":"10.1016\/j.mimet.2014.03.001","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T01:33:37Z","timestamp":1395020017000},"page":"99-104","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":34,"special_numbering":"C","title":["Real-time PCR assays for detection of Brucella spp. and the identification of genotype ST27 in bottlenose dolphins (Tursiops truncatus)"],"prefix":"10.1016","volume":"100","author":[{"given":"Qingzhong","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne E.","family":"McFee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey","family":"Goldstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah V.","family":"Tiller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Schwacke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2014.03.001_bb0010","first-page":"577","article-title":"Laboratory-based diagnosis of brucellosis\u2014a review of the literature. Part II: serological tests for brucellosis","volume":"49","author":"Al Dahouk","year":"2003","journal-title":"Clin. Lab."},{"key":"10.1016\/j.mimet.2014.03.001_bb0005","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1515\/CCLM.2007.305","article-title":"Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp.","volume":"45","author":"Al Dahouk","year":"2007","journal-title":"Clin. Chem. Lab. Med."},{"key":"10.1016\/j.mimet.2014.03.001_bb0015","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.vetmic.2008.12.023","article-title":"Real-time PCR for identification of Brucella spp.: a comparative study of IS711, bcsp31 and per target genes","volume":"137","author":"Bounaadja","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0020","first-page":"483","article-title":"Human exposure to Brucella recovered from a sea mammal","volume":"144","author":"Brew","year":"1999","journal-title":"Vet. Rec."},{"key":"10.1016\/j.mimet.2014.03.001_bb0025","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0378-1135(02)00228-6","article-title":"PCR as a diagnostic tool for brucellosis","volume":"90","author":"Bricker","year":"2002","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0030","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1128\/JCM.38.3.1258-1262.2000","article-title":"Molecular characterization of Brucella strains isolated from marine mammals","volume":"38","author":"Bricker","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0035","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mimet.2014.03.001_bb0040","author":"CFSPH"},{"key":"10.1016\/j.mimet.2014.03.001_bb0045","doi-asserted-by":"crossref","first-page":"3954","DOI":"10.1128\/JCM.05238-11","article-title":"Novel IS711 chromosomal location useful for identification of marine mammal Brucella genotype ST27, which is associated with zoonotic infection","volume":"49","author":"Cloeckaert","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0050","first-page":"868","article-title":"Occurrence and potential diagnostic applications of serological cross-reactivities between Brucella and other alpha-proteobacteria","volume":"11","author":"Delpino","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0055","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.pt.2004.08.008","article-title":"Zoonotic protozoa: from land to sea","volume":"20","author":"Fayer","year":"2004","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0060","first-page":"357","article-title":"Age, growth, and calving season of bottlenose dolphins, Tursiops truncatus, off coastal Texas","volume":"96","author":"Fernandez","year":"1998","journal-title":"Fish. Bull."},{"key":"10.1016\/j.mimet.2014.03.001_bb0065","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.1099\/ijs.0.65269-0","article-title":"Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts","volume":"57","author":"Foster","year":"2007","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0070","doi-asserted-by":"crossref","first-page":"W695","DOI":"10.1093\/nar\/gkq313","article-title":"A new bioinformatics analysis tools framework at EMBL-EBI","volume":"38","author":"Goujon","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2014.03.001_bb0075","first-page":"105","article-title":"Bacteria and fungi of marine mammals: a review","volume":"41","author":"Higgins","year":"2000","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.mimet.2014.03.001_bb0080","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1746-6148-5-22","article-title":"IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology","volume":"5","author":"Hini\u0107","year":"2009","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.mimet.2014.03.001_bb0085","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"},{"key":"10.1016\/j.mimet.2014.03.001_bb0090","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.mimet.2014.03.001_bb0095","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.micinf.2008.12.012","article-title":"Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture","volume":"11","author":"Maquart","year":"2009","journal-title":"Microbes Infect."},{"key":"10.1016\/j.mimet.2014.03.001_bb0100","doi-asserted-by":"crossref","first-page":"4363","DOI":"10.1128\/JCM.00680-06","article-title":"Characterization of a Brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand","volume":"44","author":"McDonald","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0105","first-page":"258","article-title":"Bottlenose dolphin (Tursiops truncatus) strandings in South Carolina, 1992\u20131996","volume":"100","author":"Mcfee","year":"2002","journal-title":"Fish. Bull."},{"key":"10.1016\/j.mimet.2014.03.001_bb0110","doi-asserted-by":"crossref","first-page":"575","DOI":"10.7589\/0090-3558-45.3.575","article-title":"Major pathologic findings and probable causes of mortality in bottlenose dolphins stranded in South Carolina from 1993 to 2006","volume":"45","author":"McFee","year":"2009","journal-title":"J. Wildl. Dis."},{"key":"10.1016\/j.mimet.2014.03.001_bb0115","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3354\/dao02528","article-title":"Serologic response in bottlenose dolphins Tursiops truncatus infected with Brucella sp. using a dolphin-specific indirect ELISA","volume":"102","author":"Meegan","year":"2012","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.mimet.2014.03.001_bb0120","first-page":"100","article-title":"Brucella-induced abortions and infection in bottlenose dolphins (Tursiops truncatus)","volume":"30","author":"Miller","year":"1999","journal-title":"J. Zoo Wildl. Med."},{"key":"10.1016\/j.mimet.2014.03.001_bb0125","first-page":"141","article-title":"Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9","volume":"12","author":"Mu\u0148oz","year":"2005","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0130","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1748-7692.2010.00394.x","article-title":"Prenatal data impacts common bottlenose dolphin (Tursiops truncatus) growth parameters estimated by length-at-age curves","volume":"27","author":"Neuenhoff","year":"2011","journal-title":"Mar. Mamm. Sci."},{"key":"10.1016\/j.mimet.2014.03.001_bb0135","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.ab.2006.01.051","article-title":"SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations","volume":"351","author":"Nolan","year":"2006","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mimet.2014.03.001_bb0140","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/1297-9716-42-93","article-title":"A review of Brucella infection in marine mammals, with special emphasis on Brucella pinnipedialis in the hooded seal (Cystophora cristata)","volume":"42","author":"Nymo","year":"2011","journal-title":"Vet. Res."},{"key":"10.1016\/j.mimet.2014.03.001_bb0145","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1471-2180-8-17","article-title":"Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis","volume":"8","author":"Ocampo-Sosa","year":"2008","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0150","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1177\/1040638712470945","article-title":"A multiplex real-time polymerase chain reaction assay with two internal controls for the detection of Brucella species in tissues, blood, and feces from marine mammals","volume":"25","author":"Sidor","year":"2013","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2014.03.001_bb0155","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3201\/eid0904.020576","article-title":"Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp.","volume":"9","author":"Sohn","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2014.03.001_bb0160","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/1471-2334-10-100","article-title":"Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR","volume":"10","author":"Tomaso","year":"2010","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.mimet.2014.03.001_bb0170","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1471-2180-7-34","article-title":"Characterisation of the genetic diversity of Brucella by multilocus sequencing","volume":"7","author":"Whatmore","year":"2007","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.mimet.2014.03.001_bb0165","doi-asserted-by":"crossref","first-page":"517","DOI":"10.3201\/eid1403.070829","article-title":"Marine mammal Brucella genotype associated with zoonotic infection","volume":"14","author":"Whatmore","year":"2008","journal-title":"Emerg. Infect. Dis."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701214000736?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701214000736?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:49:44Z","timestamp":1759103384000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701214000736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5]]},"references-count":34,"alternative-id":["S0167701214000736"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2014.03.001","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2014,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Real-time PCR assays for detection of Brucella spp. and the identification of genotype ST27 in bottlenose dolphins (Tursiops truncatus)","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2014.03.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}