{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T06:39:41Z","timestamp":1778827181646,"version":"3.51.4"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Canadian Federal Genomics Research and Development Initiative (GRDI)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2015,2]]},"DOI":"10.1016\/j.mimet.2014.12.003","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T02:03:13Z","timestamp":1418608993000},"page":"167-179","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["A new pyrosequencing assay for rapid detection and genotyping of Shiga toxin, intimin and O157-specific rfbE genes of Escherichia coli"],"prefix":"10.1016","volume":"109","author":[{"given":"Noriko","family":"Goji","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Mathews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Huszczynski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chad R.","family":"Laing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor P.J.","family":"Gannon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morag R.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kingsley K.","family":"Amoako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2014.12.003_bb0005","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jviromet.2010.09.026","article-title":"Detection and differentiation of tick-borne encephalitis virus subtypes by a reverse transcription quantitative real-time PCR and pyrosequencing","volume":"171","author":"Achazi","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mimet.2014.12.003_bb0010","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1101\/gr.10.8.1249","article-title":"Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing","volume":"10","author":"Alderborn","year":"2000","journal-title":"Genome Res."},{"key":"10.1016\/j.mimet.2014.12.003_bb0015","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.micinf.2006.12.001","article-title":"Hemolysin from Shiga toxin-negative Escherichia coli O26 strains injures microvascular endothelium","volume":"9","author":"Aldick","year":"2007","journal-title":"Microbes Infect."},{"key":"10.1016\/j.mimet.2014.12.003_bb0020","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1111\/j.1469-0691.2010.03203.x","article-title":"Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli","volume":"16","author":"Alperi","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2014.12.003_bb0030","doi-asserted-by":"crossref","first-page":"781652","DOI":"10.1155\/2012\/781652","article-title":"Rapid detection and identification of Yersinia pestis from food using immunomagnetic separation and pyrosequencing","volume":"2012","author":"Amoako","year":"2012","journal-title":"J. Pathog."},{"key":"10.1016\/j.mimet.2014.12.003_bb0035","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.mimet.2012.05.012","article-title":"Rapid detection and antimicrobial resistance gene profiling of Yersinia pestis using pyrosequencing technology","volume":"90","author":"Amoako","year":"2012","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2014.12.003_bb0025","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.ijfoodmicro.2013.05.028","article-title":"Rapid detection and identification of Bacillus anthracis in food using pyrosequencing technology","volume":"165","author":"Amoako","year":"2013","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0040","doi-asserted-by":"crossref","first-page":"643","DOI":"10.4315\/0362-028X.JFP-11-392","article-title":"Rapid and reliable detection of Shiga toxin-producing Escherichia coli by real-time multiplex PCR","volume":"75","author":"Anklam","year":"2012","journal-title":"J. Food Prot."},{"key":"10.1016\/j.mimet.2014.12.003_bb0045","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1089\/fpd.2011.1082","article-title":"Applicability of a multiplex PCR to detect the seven major Shiga toxin-producing Escherichia coli based on genes that code for serogroup-specific O-antigens and major virulence factors in cattle feces","volume":"9","author":"Bai","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.mimet.2014.12.003_bb0050","doi-asserted-by":"crossref","first-page":"4806","DOI":"10.1128\/AEM.00623-08","article-title":"Evaluation of major types of Shiga toxin 2E-producing Escherichia coli bacteria present in food, pigs, and the environment as potential pathogens for humans","volume":"74","author":"Beutin","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0055","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1128\/AEM.03161-13","article-title":"Intimin gene (eae) subtype-based real-time PCR strategy for specific detection of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in cattle feces","volume":"80","author":"Bibbal","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0060","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1128\/AEM.01815-08","article-title":"Detection and characterization of the fimbrial sfp cluster in enterohemorrhagic Escherichia coli O165:H25\/NM isolates from humans and cattle","volume":"75","author":"Bielaszewska","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0065","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.4315\/0362-028x.jfp-12-116","article-title":"Polyester cloth-based hybridization array system for identification of enterohemorrhagic Escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157","volume":"75","author":"Blais","year":"2012","journal-title":"J. Food Prot."},{"key":"10.1016\/j.mimet.2014.12.003_bb0070","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1128\/JCM.42.2.645-651.2004","article-title":"Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from cattle in Spain and identification of a new intimin variant gene (eae-xi)","volume":"42","author":"Blanco","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0075","doi-asserted-by":"crossref","first-page":"e85901","DOI":"10.1371\/journal.pone.0085901","article-title":"Evaluating the use of multilocus variable number tandem repeat analysis of Shiga toxin-producing Escherichia coli O157 as a routine public health tool in England","volume":"9","author":"Byrne","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2014.12.003_bb0080","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.mimet.2014.06.020","article-title":"Further development of sample preparation and detection methods for O157 and the top 6 non-O157 STEC serogroups in cattle feces","volume":"105C","author":"Conrad","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2014.12.003_bb0085","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1574-6968.2007.00755.x","article-title":"Intimin subtyping of Escherichia coli: concomitant carriage of multiple intimin subtypes from forage-fed cattle and sheep","volume":"272","author":"Cookson","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2014.12.003_bb0090","doi-asserted-by":"crossref","first-page":"e23400","DOI":"10.1371\/journal.pone.0023400","article-title":"Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing","volume":"6","author":"Deng","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2014.12.003_bb0095","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1007\/s00284-005-0447-6","article-title":"Escherichia coli O157 somatic antigen is present in an isolate of E. fergusonii","volume":"52","author":"Fegan","year":"2006","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0105","doi-asserted-by":"crossref","first-page":"6917","DOI":"10.1128\/AEM.02455-13","article-title":"Prevalences of Shiga toxin subtypes and selected other virulence factors among Shiga-toxigenic Escherichia coli strains isolated from fresh produce","volume":"79","author":"Feng","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0100","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1111\/j.1574-6968.2010.01990.x","article-title":"Prevalence, characterization and clonal analysis of Escherichia coli O157: non-H7 serotypes that carry eae alleles","volume":"308","author":"Feng","year":"2010","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2014.12.003_bb0110","doi-asserted-by":"crossref","first-page":"26","DOI":"10.2807\/1560-7917.ES2014.19.17.20787","article-title":"Emergence of Escherichia coli encoding Shiga toxin 2f in human Shiga toxin-producing E. coli (STEC) infections in the Netherlands, January 2008 to December 2011","volume":"19","author":"Friesema","year":"2014","journal-title":"Euro Surveill."},{"key":"10.1016\/j.mimet.2014.12.003_bb0115","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1128\/AAC.49.1.457-460.2005","article-title":"Detection and quantification of macrolide resistance mutations at positions 2058 and 2059 of the 23S rRNA gene by pyrosequencing","volume":"49","author":"Haanpera","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2014.12.003_bb0120","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00453-14","article-title":"PCR based detection of Shiga toxin-producing Escherichia coli (STEC) in a routine microbiology laboratory over 16years: molecular characterization of strains","author":"Haugum","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0125","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.4315\/0362-028X.JFP-13-059","article-title":"Method for the detection of priority Shiga toxin-producing Escherichia coli in beef trim","volume":"76","author":"Huszczynski","year":"2013","journal-title":"J. Food Prot."},{"key":"10.1016\/j.mimet.2014.12.003_bb0130","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1099\/jmm.0.041517-0","article-title":"Assessment of a real-time PCR for the detection and characterization of verocytotoxigenic Escherichia coli","volume":"61","author":"Jenkins","year":"2012","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0135","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli","volume":"52","author":"Joensen","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0140","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1034\/j.1600-0463.2002.100309.x","article-title":"Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments","volume":"110","author":"Jonasson","year":"2002","journal-title":"APMIS"},{"key":"10.1016\/j.mimet.2014.12.003_bb0150","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.1128\/JCM.41.11.4930-4940.2003","article-title":"Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and\/or serious disease","volume":"41","author":"Karmali","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0145","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.vetmic.2009.04.011","article-title":"Verocytotoxin-producing Escherichia coli (VTEC)","volume":"140","author":"Karmali","year":"2010","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0155","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1128\/JCM.01598-13","article-title":"Virulence gene profiles and population genetic analysis for exploration of pathogenic serogroups of Shiga toxin-producing Escherichia coli","volume":"51","author":"Kobayashi","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0160","doi-asserted-by":"crossref","first-page":"3518","DOI":"10.1099\/mic.0.2008\/019026-0","article-title":"Optical mapping and 454 sequencing of Escherichia coli O157: H7 isolates linked to the US 2006 spinach-associated outbreak","volume":"154","author":"Kotewicz","year":"2008","journal-title":"Microbiology"},{"key":"10.1016\/j.mimet.2014.12.003_bb0165","doi-asserted-by":"crossref","DOI":"10.1002\/0471142727.mb0715s104","article-title":"Pyrosequencing: powerful and quantitative sequencing technology","volume":"104","author":"Kreutz","year":"2013","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0170","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/j.1574-6968.2006.00328.x","article-title":"Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP","volume":"261","author":"Lacher","year":"2006","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2014.12.003_bb0175","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1186\/1471-2164-10-287","article-title":"In silico genomic analyses reveal three distinct lineages of Escherichia coli O157:H7, one of which is associated with hyper-virulence","volume":"10","author":"Laing","year":"2009","journal-title":"BMC Genomics"},{"key":"10.1016\/j.mimet.2014.12.003_bb0180","doi-asserted-by":"crossref","first-page":"6301","DOI":"10.1128\/AEM.02200-13","article-title":"Prevalence of hemolysin genes and comparison of ehxA subtype patterns in Shiga toxin-producing Escherichia coli (STEC) and non-STEC strains from clinical, food, and animal sources","volume":"79","author":"Lorenz","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0185","first-page":"1696","article-title":"Simplex and multiplex real-time PCR assays for the detection of flagellar (H-antigen) fliC alleles and intimin (eae) variants associated with enterohaemorrhagic Escherichia coli (EHEC) serotypes O26:H11, O103:H2, O111:H8, O145:H28 and O157:H7","volume":"109","author":"Madic","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0190","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1089\/fpd.2013.1704","article-title":"Global incidence of human Shiga toxin-producing Escherichia coli infections and deaths: a systematic review and knowledge synthesis","volume":"11","author":"Majowicz","year":"2014","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.mimet.2014.12.003_bb0195","doi-asserted-by":"crossref","first-page":"e73927","DOI":"10.1371\/journal.pone.0073927","article-title":"Enterohemorrhagic Escherichia coli as causes of hemolytic uremic syndrome in the Czech Republic","volume":"8","author":"Marejkova","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2014.12.003_bb0200","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/oxfordjournals.molbev.a026032","article-title":"Molecular evolution and mosaic structure of alpha, beta, and gamma intimins of pathogenic Escherichia coli","volume":"16","author":"McGraw","year":"1999","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0205","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.resmic.2005.03.006","article-title":"Antimicrobial resistance of Shiga toxin (verotoxin)-producing Escherichia coli O157:H7 and non-O157 strains isolated from humans, cattle, sheep and food in Spain","volume":"156","author":"Mora","year":"2005","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0210","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1128\/AEM.07520-11","article-title":"Seropathotypes, Phylogroups, Stx subtypes, and intimin types of wildlife-carried, shiga toxin-producing Escherichia coli strains with the same characteristics as human-pathogenic isolates","volume":"78","author":"Mora","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0215","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1128\/JCM.02591-06","article-title":"Subtyping method for Escherichia coli shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations","volume":"45","author":"Persson","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0220","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.ijmm.2010.06.003","article-title":"Comparative analysis of virulence genes, genetic diversity, and phylogeny of Shiga toxin 2g and heat-stable enterotoxin STIa encoding Escherichia coli isolates from humans, animals, and environmental sources","volume":"301","author":"Prager","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0225","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1101\/gr.150601","article-title":"Pyrosequencing sheds light on DNA sequencing","volume":"11","author":"Ronaghi","year":"2001","journal-title":"Genome Res."},{"key":"10.1016\/j.mimet.2014.12.003_bb0230","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1111\/j.1600-0463.2004.apm1120903.x","article-title":"Designation of O174 and O175 to temporary O groups OX3 and OX7, and six new E. coli O groups that include Verocytotoxin-producing E. coli (VTEC): O176, O177, O178, O179, O180 and O181","volume":"112","author":"Scheutz","year":"2004","journal-title":"APMIS"},{"key":"10.1016\/j.mimet.2014.12.003_bb0235","doi-asserted-by":"crossref","DOI":"10.2807\/ese.16.24.19889-en","article-title":"Characteristics of the enteroaggregative Shiga toxin\/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011","volume":"16","author":"Scheutz","year":"2011","journal-title":"Euro Surveill."},{"key":"10.1016\/j.mimet.2014.12.003_bb0240","doi-asserted-by":"crossref","first-page":"2951","DOI":"10.1128\/JCM.00860-12","article-title":"Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature","volume":"50","author":"Scheutz","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0245","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1089\/fpd.2012.1469","article-title":"Rapid identification and differentiation of non-O157 Shiga toxin-producing Escherichia coli using polymerase chain reaction coupled to electrospray ionization mass spectrometry","volume":"10","author":"Shen","year":"2013","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.mimet.2014.12.003_bb0250","doi-asserted-by":"crossref","first-page":"133","DOI":"10.3389\/fcimb.2012.00133","article-title":"Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli","volume":"2","author":"Steyert","year":"2012","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0255","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1128\/JCM.01696-12","article-title":"Public health value of next-generation DNA sequencing of enterohemorrhagic Escherichia coli isolates from an outbreak","volume":"51","author":"Underwood","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2014.12.003_bb0260","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.4315\/0362-028X.JFP-12-087","article-title":"Influence of primer sequences and DNA extraction method on detection of non-O157 Shiga toxin-producing Escherichia coli in ground beef by real-time PCR targeting the eae, stx, and serogroup-specific genes","volume":"75","author":"Wasilenko","year":"2012","journal-title":"J. Food Prot."},{"key":"10.1016\/j.mimet.2014.12.003_bb0265","doi-asserted-by":"crossref","first-page":"6445","DOI":"10.1093\/nar\/gki954","article-title":"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery","volume":"33","author":"Yang","year":"2005","journal-title":"Nucleic Acids Res."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701214003364?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701214003364?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:51:52Z","timestamp":1759103512000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701214003364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":53,"alternative-id":["S0167701214003364"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2014.12.003","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2015,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A new pyrosequencing assay for rapid detection and genotyping of Shiga toxin, intimin and O157-specific rfbE genes of Escherichia coli","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2014.12.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown copyright \u00a9 2014 Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}