{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:41:35Z","timestamp":1777495295284,"version":"3.51.4"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100010238","name":"Archer Daniels Midland Company","doi-asserted-by":"crossref","award":["20.03.2013"],"award-info":[{"award-number":["20.03.2013"]}],"id":[{"id":"10.13039\/100010238","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Scientific Center (NCN)","award":["DEC-2012\/05\/B\/NZ1\/01657"],"award-info":[{"award-number":["DEC-2012\/05\/B\/NZ1\/01657"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2016,1]]},"DOI":"10.1016\/j.mimet.2015.10.023","type":"journal-article","created":{"date-parts":[[2015,10,31]],"date-time":"2015-10-31T03:30:17Z","timestamp":1446262217000},"page":"44-49","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Development of a system for multicopy gene integration in Saccharomyces cerevisiae"],"prefix":"10.1016","volume":"120","author":[{"given":"Marta V.","family":"Semkiv","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kostyantyn V.","family":"Dmytruk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andriy A.","family":"Sibirny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2015.10.023_bb0005","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1186\/1475-2859-12-128","article-title":"Can too many copies spoil the broth?","volume":"12","author":"Aw","year":"2013","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.mimet.2015.10.023_bb0010","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0378-1119(82)90023-3","article-title":"Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5","volume":"19","author":"Beck","year":"1982","journal-title":"Gene"},{"issue":"13","key":"10.1016\/j.mimet.2015.10.023_bb0015","doi-asserted-by":"crossref","first-page":"6127","DOI":"10.1093\/nar\/16.13.6127","article-title":"High efficiency transformation of E.coli by high voltage electroporation","volume":"16","author":"Dower","year":"1988","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2015.10.023_bb0020","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0168-9525(96)10027-5","article-title":"The yeast genome project: what did we learn?","volume":"12","author":"Dujon","year":"1996","journal-title":"Trends Genet."},{"key":"10.1016\/j.mimet.2015.10.023_bb0025","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1101\/gad.4.3.324","article-title":"A specific terminal structure is required for Ty1 transposition","volume":"4","author":"Eichinger","year":"1990","journal-title":"Genes Dev."},{"key":"10.1016\/j.mimet.2015.10.023_bb0030","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1128\/jb.156.2.625-635.1983","article-title":"The presence of a defective LEU2 gene in 2pm DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number","volume":"156","author":"Erhart","year":"1983","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2015.10.023_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0124459","article-title":"Synthesis of morphinan alkaloids in Saccharomyces cerevisiae","volume":"10","author":"Fossati","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2015.10.023_bb0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.copbio.2014.09.004","article-title":"Metabolic engineering of Saccharomyces cerevisiae for production of butanol isomers","volume":"33","author":"Generoso","year":"2015","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.mimet.2015.10.023_bb0045","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1128\/jb.153.1.163-168.1983","article-title":"Transformation of intact yeast cells treated with alkali cations","volume":"153","author":"Ito","year":"1983","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2015.10.023_bb0050","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/1567-1364.12118","article-title":"EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae","volume":"14","author":"Jensen","year":"2014","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.mimet.2015.10.023_bb0055","doi-asserted-by":"crossref","first-page":"477","DOI":"10.2144\/000112730","article-title":"\u0392-lactamase reporter system for selecting high-producing yeast clones","volume":"44","author":"Hribar","year":"2008","journal-title":"Biotechniques"},{"issue":"2","key":"10.1016\/j.mimet.2015.10.023_bb0060","first-page":"127","article-title":"Identification of the genetic locus for the structural gene and a new regulatory gene for the synthesis of repressible alkaline phosphatase in Saccharomyces cerevisiae","volume":"2","author":"Kaneko","year":"1982","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.mimet.2015.10.023_bb0065","doi-asserted-by":"crossref","first-page":"395","DOI":"10.4161\/bbug.1.6.13257","article-title":"Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanism","volume":"1","author":"Kawai","year":"2010","journal-title":"Bioeng. Bugs"},{"key":"10.1016\/j.mimet.2015.10.023_bb0070","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s002530051059","article-title":"Improved efficiency and stability of multiple cloned gene insertions at the\u03b4 sequences of Saccharomyces cerevisiae","volume":"48","author":"Lee","year":"1997","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2015.10.023_bb0075","doi-asserted-by":"crossref","unstructured":"Lopes T.S., Klootwijk J., Veenstra A.E., Aar P.C. van der, Heerikhuizen H. van, Raue H.A., Planta R.J., 1989. High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression. Gene 79, 199\u2013206.","DOI":"10.1016\/0378-1119(89)90202-3"},{"key":"10.1016\/j.mimet.2015.10.023_bb0080","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0378-1119(91)90516-E","article-title":"Mechanism of high-copy-number integration of pMIRY-type vectors into the ribosomal DNA of Saccharomyces cerevisiae","volume":"105","author":"Lopes","year":"1991","journal-title":"Gene"},{"key":"10.1016\/j.mimet.2015.10.023_bb0085","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/(SICI)1097-0061(199604)12:5<467::AID-YEA933>3.0.CO;2-3","article-title":"Factors affecting the mitotic stability of high-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae","author":"Lopes","year":"1996","journal-title":"Yeast 12"},{"key":"10.1016\/j.mimet.2015.10.023_bb0090","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/0092-8674(83)90553-6","article-title":"Pedigree analysis of plasmid segregation in yeast","volume":"34","author":"Murray","year":"1983","journal-title":"Cell"},{"issue":"6","key":"10.1016\/j.mimet.2015.10.023_bb0095","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.ymben.2009.07.005","article-title":"Engineering of Saccharomyces cerevisiae for the production of dihydroxyacetone (DHA) from sugars: a proof of concept","volume":"11","author":"Nguyen","year":"2009","journal-title":"Metab. Eng."},{"key":"10.1016\/j.mimet.2015.10.023_bb0100","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1263\/jbb.103.318","article-title":"Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger beta-galactosidase production","volume":"103","author":"Oliveira","year":"2007","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.mimet.2015.10.023_bb0105","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1128\/MMBR.64.1.34-50.2000","article-title":"Metabolic engineering of Saccharomyces cerevisiae","volume":"64","author":"Ostergaard","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.mimet.2015.10.023_bb0110","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1021\/bp9500627","article-title":"An integration vector for tunable, high copy, stable integration into the dispersed Ty \u03b4 sites of Saccharomyces cerevisiae","volume":"12","author":"Parekh","year":"1996","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.mimet.2015.10.023_bb0115","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/yea.320080602","article-title":"Foreign gene expression in yeast: a review","volume":"8","author":"Romanos","year":"1992","journal-title":"Yeast"},{"key":"10.1016\/j.mimet.2015.10.023_bb0120","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/BF00340712","article-title":"High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier","volume":"16","author":"Schiestl","year":"1989","journal-title":"Curr. Genet."},{"key":"10.1016\/j.mimet.2015.10.023_bb0125","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1472-6750-14-42","article-title":"Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiae overexpressing alkaline phosphatase","volume":"14","author":"Semkiv","year":"2014","journal-title":"BMC Biotechnol."},{"key":"10.1016\/j.mimet.2015.10.023_bb0130","doi-asserted-by":"crossref","first-page":"73","DOI":"10.2144\/000112040","article-title":"System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae","volume":"40","author":"Taxis","year":"2006","journal-title":"Biotechniques"},{"key":"10.1016\/j.mimet.2015.10.023_bb0135","first-page":"1","article-title":"Production of farnesene and santalene by Saccharomyces cerevisiae using fed-batch cultivations with RQ-controlled feed","volume":"9999","author":"Tippmann","year":"2015","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.mimet.2015.10.023_bb0140","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1002\/(SICI)1097-0290(19960105)49:1<45::AID-BIT6>3.0.CO;2-T","article-title":"G418 selection and stability of cloned genes integrated at the chromosomal d sequences of Saccharomyces cerevisiae","volume":"49","author":"Wang","year":"1996","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.mimet.2015.10.023_bb0145","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0734-9750(01)00060-X","article-title":"Glycerol production by microbial fermentation: a review","volume":"19","author":"Wang","year":"2001","journal-title":"Biotech. Adv."},{"issue":"9","key":"10.1016\/j.mimet.2015.10.023_bb0150","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1128\/AEM.02591-07","article-title":"Malic acid production by Saccharomyces cerevisiae: engineering of pyruvate carboxylation, oxaloacetate reduction, and malate export","volume":"74","author":"Zelle","year":"2008","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701215301019?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701215301019?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:51:44Z","timestamp":1759103504000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701215301019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":30,"alternative-id":["S0167701215301019"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2015.10.023","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2016,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a system for multicopy gene integration in Saccharomyces cerevisiae","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2015.10.023","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}