{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T16:17:44Z","timestamp":1774628264228,"version":"3.50.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2016,1]]},"DOI":"10.1016\/j.mimet.2015.11.010","type":"journal-article","created":{"date-parts":[[2015,11,12]],"date-time":"2015-11-12T09:33:24Z","timestamp":1447320804000},"page":"15-22","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["High dynamic range detection of Chlamydia trachomatis growth by direct quantitative PCR of the infected cells"],"prefix":"10.1016","volume":"120","author":[{"given":"Ildik\u00f3","family":"Eszik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ildik\u00f3","family":"Lantos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamil","family":"\u00d6nder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferenc","family":"Somogyv\u00e1ri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Buri\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria","family":"Endr\u00e9sz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezs\u0151 P.","family":"Virok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2015.11.010_bb0005","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.yexmp.2015.01.011","article-title":"A multiplex PCR assay for the simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis","volume":"98","author":"Abou Tayoun","year":"2015","journal-title":"Exp. Mol. Pathol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0010","doi-asserted-by":"crossref","first-page":"15971","DOI":"10.1073\/pnas.2535394100","article-title":"Transcriptome analysis of chlamydial growth during IFN-gamma-mediated persistence and reactivation","volume":"100","author":"Belland","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mimet.2015.11.010_bb0015","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/AAC.01400-13","article-title":"Application of DNA chip scanning technology for automatic detection of Chlamydia trachomatis and Chlamydia pneumoniae inclusions","volume":"58","author":"Bogdanov","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2015.11.010_bb0020","author":"CDC Sexually Transmitted Disease Surveillance"},{"key":"10.1016\/j.mimet.2015.11.010_bb0025","first-page":"252","article-title":"Diagnostic efficacy of a real time-PCR assay for Chlamydia trachomatis infection in infertile women in north India","volume":"140","author":"Dhawan","year":"2014","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.mimet.2015.11.010_bb0030","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1093\/jac\/43.6.825","article-title":"Comparative in-vitro activity of moxifloxacin, minocycline and azithromycin against Chlamydia spp.","volume":"43","author":"Donati","year":"1999","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2015.11.010_bb0035","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1056\/NEJMoa041681","article-title":"Effect of expedited treatment of sex partners on recurrent or persistent gonorrhea or chlamydial infection","volume":"352","author":"Golden","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.mimet.2015.11.010_bb0040","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1128\/AAC.00046-09","article-title":"Antibiotic susceptibility of Waddlia chondrophila in Arcanthamoeba castellanii amoebae","volume":"53","author":"Goy","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"issue":"Suppl. 2","key":"10.1016\/j.mimet.2015.11.010_bb0045","doi-asserted-by":"crossref","first-page":"S134","DOI":"10.1086\/652395","article-title":"Risk of sequelae after Chlamydia trachomatis genital infection in women","volume":"201","author":"Haggerty","year":"2010","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.mimet.2015.11.010_bb0050","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.ultsonch.2010.07.015","article-title":"Synergistic effect of ultrasound and antibiotics against Chlamydia trachomatis-infected human epithelial cells in vitro","volume":"18","author":"Ikeda-Dantsuji","year":"2011","journal-title":"Ultrason. Sonochem."},{"key":"10.1016\/j.mimet.2015.11.010_bb0055","series-title":"Proc. Ninth Int. Symp. Hum. Chlamydial Infect. USA Int. Chlamydia Symp. San Franc.","first-page":"35","article-title":"Factors affecting chlamydial persistence or recurrence one and three months after treatment. Chlamydial infections","author":"Katz","year":"1998"},{"key":"10.1016\/j.mimet.2015.11.010_bb0060","doi-asserted-by":"crossref","DOI":"10.1136\/bmj.c1642","article-title":"Randomised controlled trial of screening for Chlamydia trachomatis to prevent pelvic inflammatory disease: the POPI (prevention of pelvic infection) trial","volume":"340","author":"Oakeshott","year":"2010","journal-title":"BMJ"},{"key":"10.1016\/j.mimet.2015.11.010_bb0065","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1128\/AAC.00056-13","article-title":"Simple resazurin-based microplate assay for measuring chlamydia infections","volume":"57","author":"Osaka","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2015.11.010_bb0070","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1128\/AAC.00427-12","article-title":"An automated image-based method for rapid analysis of chlamydia infection as a tool for screening antichlamydial agents","volume":"56","author":"Osaka","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2015.11.010_bb0075","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1111\/j.1365-2958.2006.05465.x","article-title":"Global transcriptional upregulation in the absence of increased translation in chlamydia during IFNgamma-mediated host cell tryptophan starvation","volume":"62","author":"Ouellette","year":"2006","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0080","doi-asserted-by":"crossref","DOI":"10.1111\/apm.12391","article-title":"Detection of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in infertile Bulgarian men with multiplex real-time polymerase chain reaction","author":"Ouzounova-Raykova","year":"2015","journal-title":"APMIS"},{"key":"10.1016\/j.mimet.2015.11.010_bb0085","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1099\/jmm.0.023887-0","article-title":"Effects of antibiotics on Chlamydia trachomatis viability as determined by real-time quantitative PCR","volume":"60","author":"Peuchant","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0090","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1099\/jmm.0.023887-0","article-title":"Effects of antibiotics on Chlamydia trachomatis viability as determined by real-time quantitative PCR","volume":"60","author":"Peuchant","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0095","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1128\/jcm.20.2.217-222.1984","article-title":"Enhancement of Chlamydia trachomatis infectious progeny by cultivation of HeLa 229 cells treated with DEAE-dextran and cycloheximide","volume":"20","author":"Sabet","year":"1984","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0100","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0732-8893(01)00221-8","article-title":"In vitro susceptibility of recent clinical isolates of Chlamydia trachomatis to macrolides and tetracyclines","volume":"39","author":"Samra","year":"2001","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2015.11.010_bb0105","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1128\/AAC.01655-10","article-title":"Impact of a low-oxygen environment on the efficacy of antimicrobials against intracellular Chlamydia trachomatis","volume":"55","author":"Shima","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2015.11.010_bb0110","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.1128\/CVI.00460-12","article-title":"Fluorometric high-throughput assay for measuring chlamydial neutralizing antibody","volume":"19","author":"Southern","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0115","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1093\/nar\/gkh271","article-title":"A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro","volume":"32","author":"Wang","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2015.11.010_bb0120","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1099\/0022-1317-51-12-1021","article-title":"The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review","volume":"51","author":"Watson","year":"2002","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2015.11.010_bb0125","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/AAC.36.2.291","article-title":"In vitro evaluation of activities of azithromycin, erythromycin, and tetracycline against Chlamydia trachomatis and Chlamydia pneumoniae","volume":"36","author":"Welsh","year":"1992","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2015.11.010_bb0130","author":"WHO | Trachoma Fact sheet No 382, http:\/\/www.who.int\/mediacentre\/factsheets\/fs382\/en\/ [WWW Document]"},{"key":"10.1016\/j.mimet.2015.11.010_bb0135","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.mimet.2015.11.010_bb0140","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/1471-2105-7-85","article-title":"Statistical analysis of real-time PCR data","volume":"7","author":"Yuan","year":"2006","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.mimet.2015.11.010_bb0145","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1097\/OLQ.0000000000000241","article-title":"Association of the in vitro susceptibility of clinical isolates of Chlamydia trachomatis with serovar and duration of antibiotic exposure","volume":"42","author":"Zheng","year":"2015","journal-title":"Sex. Transm. Dis."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701215301135?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701215301135?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:51:21Z","timestamp":1759103481000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701215301135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":29,"alternative-id":["S0167701215301135"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2015.11.010","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2016,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High dynamic range detection of Chlamydia trachomatis growth by direct quantitative PCR of the infected cells","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2015.11.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}