{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:43:52Z","timestamp":1790833432800,"version":"4.1.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307447\/2014-6"],"award-info":[{"award-number":["307447\/2014-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.mimet.2016.08.017","type":"journal-article","created":{"date-parts":[[2016,8,22]],"date-time":"2016-08-22T19:18:19Z","timestamp":1471893499000},"page":"98-102","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":73,"special_numbering":"C","title":["Giardiasis: an update review on sensitivity and specificity of methods for laboratorial diagnosis"],"prefix":"10.1016","volume":"129","author":[{"given":"Renata","family":"Soares","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8334-5105","authenticated-orcid":false,"given":"Tiana","family":"Tasca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.mimet.2016.08.017_bb0005","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1128\/CMR.14.3.447-475.2001","article-title":"Biology of Giardia lamblia","volume":"14","author":"Adam","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"issue":"4","key":"10.1016\/j.mimet.2016.08.017_bb0010","doi-asserted-by":"crossref","first-page":"362","DOI":"10.26719\/2010.16.4.362","article-title":"Detection of Giardia lamblia antigen in stool specimens using enzyme-linked immunosorbent assay","volume":"16","author":"Al-Saeed","year":"2010","journal-title":"East Mediterr. Health J."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0015","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1007\/s00436-011-2565-3","article-title":"Evaluation of a PCR protocol for sensitive detection of Giardia intestinalis in human faeces","volume":"110","author":"Asher","year":"2012","journal-title":"Parasitol. Res."},{"issue":"9","key":"10.1016\/j.mimet.2016.08.017_bb0020","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1136\/jclinpath-2014-202224","article-title":"A critical assessment of two real-time PCR assays targeting the (SSU) rRna and gdh genes for the molecular identification of Giardia intestinalis in a clinical laboratory","volume":"67","author":"Boadi","year":"2014","journal-title":"J. Clin. Pathol."},{"issue":"3","key":"10.1016\/j.mimet.2016.08.017_bb0025","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.diagmicrobio.2009.10.004","article-title":"Evaluation of a real-time polymerase chain reaction assay for the laboratory diagnosis of giardiasis","volume":"66","author":"Calderaro","year":"2010","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0030","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.cll.2015.02.010","article-title":"Infections by intestinal coccidia and Giardia intestinalis","volume":"35","author":"Cama","year":"2015","journal-title":"Clin. Lab. Med."},{"key":"10.1016\/j.mimet.2016.08.017_bb0035","author":"Centers for Disease Control and Prevention"},{"issue":"5","key":"10.1016\/j.mimet.2016.08.017_bb0040","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1128\/JCM.06483-11","article-title":"Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of Giardia, Cryptosporidium and Entamoeba histolytica antigens in human stool","volume":"50","author":"Christy","year":"2012","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.mimet.2016.08.017_bb0045","doi-asserted-by":"crossref","first-page":"754","DOI":"10.5858\/2005-129-754-SFCIUA","article-title":"Screening for Giardia\/Cryptosporidium infections using an enzyme immunoassay in a centralized regional microbiology laboratory","volume":"129","author":"Church","year":"2005","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.mimet.2016.08.017_bb0050","series-title":"Procedures for the Recovery and Identification of Parasites from the Intestinal Tract; Approved Guideline. CLSI Document M28-A2","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2005"},{"issue":"1","key":"10.1016\/j.mimet.2016.08.017_bb0055","doi-asserted-by":"crossref","first-page":"167","DOI":"10.4269\/ajtmh.2012.12-0142","article-title":"Diagnosis of multiple enteric protozoan infections by enzyme-linked immunosorbent assay in the Guatemalan highlands","volume":"88","author":"den Hartog","year":"2013","journal-title":"Am.J.Trop. Med. Hyg."},{"issue":"3","key":"10.1016\/j.mimet.2016.08.017_bb0060","doi-asserted-by":"crossref","first-page":"169","DOI":"10.5152\/tpd.2013.38","article-title":"Comparison of direct microscopy and antigen cassette tests for the detection of Giardia and Cryptosporidium","volume":"37","author":"Doni","year":"2014","journal-title":"Turkiye Parazitol. Derg."},{"issue":"1","key":"10.1016\/j.mimet.2016.08.017_bb0065","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1002\/cyto.b.21048","article-title":"Giardia diagnostic methods in human fecal samples: a comparative study","volume":"84","author":"El-Nahas","year":"2013","journal-title":"Cytometry B Clin. Cytom."},{"issue":"4","key":"10.1016\/j.mimet.2016.08.017_bb0070","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1007\/s00436-013-3319-1","article-title":"Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum","volume":"112","author":"Elsafi","year":"2013","journal-title":"Parasitol. Res."},{"issue":"1","key":"10.1016\/j.mimet.2016.08.017_bb0075","first-page":"1","article-title":"Diagnosis of Giardia and Cryptosporidium infection in children with diarrheal illness using rapid immunochromatographic assay","volume":"3","author":"El-Tantawy","year":"2012","journal-title":"Arch. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2016.08.017_bb0080","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1128\/CMR.00033-10","article-title":"Zoonotic potential and molecular epidemiology of Giardia species and giardiasis","volume":"24","author":"Feng","year":"2011","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"10.1016\/j.mimet.2016.08.017_bb0085","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s13149-015-0434-5","article-title":"Evaluation of the new Immunocard STAT! \u00ae CGE test for the diagnosis of Amebiasis","volume":"108","author":"Formenti","year":"2015","journal-title":"Bull. Soc. Pathol. Exot."},{"issue":"8","key":"10.1016\/j.mimet.2016.08.017_bb0090","doi-asserted-by":"crossref","first-page":"e541","DOI":"10.1016\/j.ijid.2011.04.004","article-title":"Evaluation of enzyme immunoassay techniques for diagnosis of the most common intestinal protozoa in fecal samples","volume":"15","author":"Gaafar","year":"2011","journal-title":"Int. J. Infect. Dis."},{"issue":"12","key":"10.1016\/j.mimet.2016.08.017_bb0095","doi-asserted-by":"crossref","first-page":"4587","DOI":"10.1128\/JCM.01458-06","article-title":"Detection of Giardia lamblia antigens in human fecal specimens by a solid-phase qualitative immunochromatographic assay","volume":"44","author":"Garcia","year":"2006","journal-title":"J. Clin. Microbiol."},{"issue":"8","key":"10.1016\/j.mimet.2016.08.017_bb0100","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1007\/s10096-012-1544-7","article-title":"Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp., Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples","volume":"31","author":"Go\u00f1i","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mimet.2016.08.017_bb0105","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1590\/S0034-89102002000100010","article-title":"Detection of anti-Giardia lamblia serum antibody among children of day care centers","volume":"36","author":"Guimar\u00e3es","year":"2002","journal-title":"Rev. Saude Publica"},{"issue":"3","key":"10.1016\/j.mimet.2016.08.017_bb0110","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.eimc.2010.09.005","article-title":"Assessment of two commercially available immunochromatographic assays for a rapid diagnosis of Giardia duodenalis and Crypstosporidium spp. in human fecal specimens","volume":"29","author":"Guti\u00e9rrez-Cisneros","year":"2011","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"10.1016\/j.mimet.2016.08.017_bb0115","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/213745","article-title":"Evaluation of an immunoassay-based algorithm for screening and identification of Giardia and Cryptosporidium antigens in human faecal specimens from Saudi Arabia","volume":"2014","author":"Hawash","year":"2014","journal-title":"J. Parasitol Res."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0120","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.gtc.2006.03.006","article-title":"An updated review on Cryptosporidium and Giardia","volume":"35","author":"Huang","year":"2006","journal-title":"Gastroenterol. Clin. N. Am."},{"issue":"11","key":"10.1016\/j.mimet.2016.08.017_bb0125","first-page":"DC04","article-title":"A comparison of microscopy and enzyme linked immunosorbent assay for diagnosis of Giardia lamblia in human faecal specimens","volume":"8","author":"Jahan","year":"2014","journal-title":"J. Clin. Diagn."},{"issue":"15","key":"10.1016\/j.mimet.2016.08.017_bb0130","doi-asserted-by":"crossref","first-page":"5420","DOI":"10.1128\/AEM.00416-11","article-title":"Rapid detection and enumeration of Giardia lamblia cysts in water samples by immunomagnetic separation and flow cytometric analysis","volume":"77","author":"Keserue","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0135","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.biotechadv.2013.10.009","article-title":"Giardia\/giardiasis \u2013 a perspective on diagnostic and analytical tools","volume":"32","author":"Koehler","year":"2014","journal-title":"Biotechnol. Adv."},{"issue":"8","key":"10.1016\/j.mimet.2016.08.017_bb0140","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/BF02447929","article-title":"1997. Sensivity of microscopy versus enzyme immunoassay in the laboratory diagnosis of giardiasis","volume":"16","author":"Mank","year":"1997","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"4","key":"10.1016\/j.mimet.2016.08.017_bb0145","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s10096-011-1332-9","article-title":"Evaluation of an immunochromatographic assay: Giardia-Strip\u00ae (Coris BioConcept) for detection of Giardia intestinalis in human fecal specimens","volume":"31","author":"Nguyen","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0150","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s10096-006-0088-0","article-title":"Evaluation of the immunochromatographic coris giardia-strip test for rapid diagnosis of Giardia lamblia","volume":"25","author":"Oster","year":"2006","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2016.08.017_bb0155","first-page":"15","article-title":"Centers for Disease Control and Prevention. Giardiasis surveillance \u2013 United States, 2011\u20132012","volume":"64","author":"Painter","year":"2015","journal-title":"MMWR Surveill. Summ."},{"issue":"Pt 1","key":"10.1016\/j.mimet.2016.08.017_bb0160","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1017\/S0031182005007353","article-title":"Asymptomatic giardiasis and growth in young children; a longitudinal study in Salvador, Brazil","volume":"131","author":"Prado","year":"2005","journal-title":"Parasitology"},{"key":"10.1016\/j.mimet.2016.08.017_bb0165","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.meegid.2015.03.015","article-title":"Molecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area incentral Colombia","volume":"32","author":"Ram\u00edrez","year":"2015","journal-title":"Infect. Genet."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0170","doi-asserted-by":"crossref","first-page":"399","DOI":"10.2166\/wh.2009.172","article-title":"Detection of Giardia and Cryptosporidium cysts\/oocysts in watersheds and drinking water sources in Brazil urban areas","volume":"8","author":"Razzolini","year":"2010","journal-title":"J. Water Health"},{"issue":"8","key":"10.1016\/j.mimet.2016.08.017_bb0175","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1007\/s00436-015-4486-z","article-title":"Evaluation of immunocard Stat Test and ELISA versus light microscopy in diagnosis of giardiasis and cryptosporidiosis","volume":"114","author":"Sadaka","year":"2015","journal-title":"Parasitol. Res."},{"issue":"8","key":"10.1016\/j.mimet.2016.08.017_bb0180","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.trstmh.2012.05.011","article-title":"Epidemiological, parasitological and molecular aspects of Giardia duodenalis infection in children attending public daycare centers in southeastern Brazil","volume":"106","author":"Santos","year":"2012","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"12","key":"10.1016\/j.mimet.2016.08.017_bb0185","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1111\/j.1469-0691.2007.01836.x","article-title":"Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens","volume":"13","author":"Schuurman","year":"2007","journal-title":"Clin. Microbiol. Infect."},{"issue":"1","key":"10.1016\/j.mimet.2016.08.017_bb0190","doi-asserted-by":"crossref","first-page":"69","DOI":"10.4103\/0377-4929.151192","article-title":"Comparative analysis of enzyme-linked immunosorbent assay and direct microscopy for the diagnosis of Giardia intestinalis in fecal samples","volume":"58","author":"Singhal","year":"2015","journal-title":"Indian J. Pathol. Microbiol."},{"issue":"Suppl 4","key":"10.1016\/j.mimet.2016.08.017_bb0195","first-page":"26","article-title":"Re-evaluation commercially available enzyme-linked immunosorbent assay for the detection of Giardia lamblia and Cryptosporidium spp. from stool specimens","volume":"36","author":"Srijan","year":"2005","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.mimet.2016.08.017_bb0200","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/564721","article-title":"Comparison of the triage micro parasite panel and microscopy for the detection of Entamoeba histolytica\/Entamoeba d\u00edspar, Giardia lamblia, and Crypstosporidium parvum in stool samples collected in Kenya","volume":"2012","author":"Swierczewski","year":"2012","journal-title":"J. Trop. Med."},{"key":"10.1016\/j.mimet.2016.08.017_bb0205","doi-asserted-by":"crossref","first-page":"S725","DOI":"10.1016\/S2222-1808(14)60715-5","article-title":"Consistency of direct microscopic examination and ELISA in detection of Giardia in stool specimen among children","volume":"4","author":"Torabi","year":"2014","journal-title":"Asian Pacific J. Trop. Dis."},{"key":"10.1016\/j.mimet.2016.08.017_bb0210","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.foodcont.2015.06.007","article-title":"Development of a qRT-PCR method to assess the viability of Giardia intestinalis cysts, Cryptosporidium spp. and Toxoplasma gondii oocysts","volume":"59","author":"Travaill\u00e9","year":"2016","journal-title":"Food Control"},{"key":"10.1016\/j.mimet.2016.08.017_bb0215","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.mimet.2015.01.016","article-title":"Comparison of four rapid diagnostic tests, ELISA, microscopy and PCR for the detection of Giardia lamblia, Cryptosporidium spp. and Entamoeba histolytica in feces","volume":"110","author":"Van den Bossche","year":"2015","journal-title":"J. Microbiol. Methods"},{"issue":"6","key":"10.1016\/j.mimet.2016.08.017_bb0220","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1590\/S1516-31802005000600006","article-title":"2005. Enzyme-linked immunosorbent assay (ELISA) immunoassaying versus microscopy: advantages and drawbacks for diagnosing giardiasis","volume":"123","author":"Vidal","year":"2005","journal-title":"S\u00e3o Paulo Med. J."},{"issue":"7","key":"10.1016\/j.mimet.2016.08.017_bb0225","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1111\/j.1469-0691.2006.01457.x","article-title":"Evaluation of seven commercial antigen detection tests for Giardia and Cryptosporidium in stool samples","volume":"12","author":"Weitzel","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2016.08.017_bb0230","author":"World Health Organization"},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0235","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.idc.2012.03.009","article-title":"Protozoan infections of the gastrointestinal tract","volume":"26","author":"Wright","year":"2012","journal-title":"Infect. Dis. Clin. N. Am."},{"issue":"2","key":"10.1016\/j.mimet.2016.08.017_bb0240","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1128\/JCM.01736-08","article-title":"Evaluation of a screening test for detection of Giardia and Cryptosporidium parasites","volume":"47","author":"Youn","year":"2009","journal-title":"J. Clin. Microbiol."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770121630224X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770121630224X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:53:29Z","timestamp":1759103609000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016770121630224X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":48,"alternative-id":["S016770121630224X"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2016.08.017","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Giardiasis: an update review on sensitivity and specificity of methods for laboratorial diagnosis","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2016.08.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}