{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:21:55Z","timestamp":1773886915995,"version":"3.50.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1016\/j.mimet.2016.10.017","type":"journal-article","created":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T15:31:01Z","timestamp":1477323061000},"page":"130-134","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Development of an endpoint genotyping assay to detect the Mycoplasma pneumoniae 23S rRNA gene and distinguish the existence of macrolide resistance-associated mutations at position 2063"],"prefix":"10.1016","volume":"131","author":[{"given":"Yu","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Seto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitaka","family":"Shimotai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazue","family":"Yahagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Mizuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Matsuzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Hongo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2016.10.017_bb0005","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1111\/j.1574-6976.2008.00129.x","article-title":"Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections","volume":"32","author":"Atkinson","year":"2008","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.mimet.2016.10.017_bb0010","first-page":"20","article-title":"Detection of macrolide resistant Mycoplasma pneumoniae in England, September 2014 to September 2015","author":"Brown","year":"2015","journal-title":"Eurosurveil"},{"key":"10.1016\/j.mimet.2016.10.017_bb0015","first-page":"75","article-title":"Comparison between two real-time PCR assay and a nested-PCR for the detection of Toxoplasma gondii","volume":"77","author":"Calderaro","year":"2006","journal-title":"Acta Biomed"},{"key":"10.1016\/j.mimet.2016.10.017_bb0020","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1093\/jac\/dkr003","article-title":"Emergence of macrolide-resistant strains during an outbreak of Mycoplasma pneumoniae infections in children","volume":"66","author":"Chironna","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0025","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1469-0691.2009.02968.x","article-title":"Occurrence of macrolide-resistant Mycoplasma pneumoniae pstrains in Germany","volume":"16","author":"Dumke","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2016.10.017_bb0030","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1128\/JVI.01367-10","article-title":"Role of the CM2 protein in the influenza C virus replication cycle","volume":"85","author":"Furukawa","year":"2011","journal-title":"J. Virol."},{"key":"10.1016\/j.mimet.2016.10.017_bb0035","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.3760\/cma.j.issn.0366-6999.20131654","article-title":"Surveillance of Mycoplasma pneumoniae infection among children in Beijing from 2007 to 2012","volume":"127","author":"Hanqing","year":"2014","journal-title":"Chin. Med. J."},{"key":"10.1016\/j.mimet.2016.10.017_bb0040","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.3201\/eid1908.121455","article-title":"Macrolide resistance of Mycoplasma pneumoniae, South Korea, 2000\u20132011","volume":"19","author":"Hong","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2016.10.017_bb0045","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.mimet.2014.04.009","article-title":"Single-nucleotide polymorphism PCR for the detection of Mycoplasma pneumoniae and determination of macrolide resistance in respiratory samples","volume":"102","author":"Ji","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2016.10.017_bb0050","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1348-0421.2004.tb03530.x","article-title":"Comparison of real-time and nested PCR assay for detection oh herpes simplex virus DNA","volume":"48","author":"Kawada","year":"2004","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.mimet.2016.10.017_bb0055","doi-asserted-by":"crossref","first-page":"4046","DOI":"10.1128\/AAC.00663-13","article-title":"Nationwide surveillance of macrolide-resistant Mycoplasma pneumoniae infection in pediatric patients","volume":"57","author":"Kawai","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0060","first-page":"2671","article-title":"A single tube modified allele-specific-PCR for rapid detection of erythromycin-resistant Mycoplasma pneumoniae in Beijing","volume":"125","author":"Li","year":"2012","journal-title":"Chin. Med. J."},{"key":"10.1016\/j.mimet.2016.10.017_bb0065","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.diagmicrobio.2010.03.004","article-title":"Characterization of macrolide-resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China","volume":"67","author":"Liu","year":"2010","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2016.10.017_bb0070","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.diagmicrobio.2013.12.002","article-title":"Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by cycleave PCR","volume":"78","author":"Liu","year":"2014","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2016.10.017_bb0075","doi-asserted-by":"crossref","first-page":"4624","DOI":"10.1128\/AAC.48.12.4624-4630.2004","article-title":"Characterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan","volume":"48","author":"Matsuoka","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0080","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/AAC.00779-07","article-title":"The acute respiratory diseases study group. Increased macrolide resistance of Mycoplasma pneumoniae in pediatric patients with community-acquired pneumonia","volume":"52","author":"Morozumi","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0085","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s10156-009-0021-4","article-title":"Macrolide-resistant Mycoplasma pneumoniae: characteristics of isolates and clinical aspects of community-acquired pneumonia","volume":"16","author":"Morozumi","year":"2010","journal-title":"J. Infect. Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0090","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1002\/jmv.22101","article-title":"Rapid discrimination of oseltamivir-resistant 275Y and -susceptible 275H substitutions in the neuraminidase gene of pandemic influenza A\/H1N1 2009 virus by duplex one-step RT-PCR assay","volume":"83","author":"Nakauchi","year":"2011","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.mimet.2016.10.017_bb0095","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/j.1348-0421.2001.tb01293.x","article-title":"Characteristics of macrolide-resistant Mycoplasma pneumoniae strains isolated from patients and induced with erythromycin in vitro","volume":"45","author":"Okazaki","year":"2001","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.mimet.2016.10.017_bb0100","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1128\/AAC.48.2.460-465.2004","article-title":"In vitro selection and characterization of resistance to macrolides and related antibiotics in Mycoplasma pneumoniae","volume":"48","author":"Pereyre","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0105","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/jac\/dkp160","article-title":"Increased macrolide resistance of Mycoplasma pneumoniae in France directly detected in clinical specimens by real-time PCR and melting curve analysis","volume":"64","author":"Peuchant","year":"2009","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0110","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.mimet.2010.06.004","article-title":"Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae by pyrosequencing","volume":"82","author":"Spuesens","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2016.10.017_bb0115","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1097\/INF.0b013e31827aa7bd","article-title":"Community outbreak of macrolide-resistant Mycoplasma pneumoniae in Yamagata, Japan in 2009","volume":"32","author":"Suzuki","year":"2013","journal-title":"Pediatr. Infect. Dis."},{"key":"10.1016\/j.mimet.2016.10.017_bb0120","doi-asserted-by":"crossref","first-page":"16","DOI":"10.11150\/kansenshogakuzasshi.89.16","article-title":"Gene mutations associated with macrolide-resistance p1 gene typing of Mycoplasma pneumoniae isolated in Yamagata, Japan, between 2004 and 2013","volume":"89","author":"Suzuki","year":"2015","journal-title":"Kansenshogaku Zasshi"},{"key":"10.1016\/j.mimet.2016.10.017_bb0125","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2010.11.014","article-title":"Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay","volume":"70","author":"Thurman","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2016.10.017_bb0130","first-page":"337","article-title":"Large epidemic of mycoplasmal pneumonia caused by Mycoplasma pneumoniae strains highly resistant to macrolides among children in 2011","volume":"32","author":"Ubukata","year":"2011","journal-title":"IASR"},{"key":"10.1016\/j.mimet.2016.10.017_bb0135","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/CMR.17.4.697-728.2004","article-title":"Mycoplasma pneumoniae and its role as a human pathogen","volume":"17","author":"Waites","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.mimet.2016.10.017_bb0140","doi-asserted-by":"crossref","first-page":"3542","DOI":"10.1128\/AAC.00582-08","article-title":"Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis","volume":"52","author":"Wolff","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2016.10.017_bb0145","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.3201\/eid2108.150273","article-title":"Macrolide-resistant Mycoplasma pneumoniae, United States","volume":"21","author":"Zheng","year":"2015","journal-title":"Emerg. Infect. Dis."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701216303050?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701216303050?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:54:33Z","timestamp":1759103673000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701216303050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":29,"alternative-id":["S0167701216303050"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2016.10.017","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2016,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of an endpoint genotyping assay to detect the Mycoplasma pneumoniae 23S rRNA gene and distinguish the existence of macrolide resistance-associated mutations at position 2063","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2016.10.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}