{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T10:24:34Z","timestamp":1778495074630,"version":"3.51.4"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1016\/j.mimet.2017.08.009","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T12:45:36Z","timestamp":1502714736000},"page":"90-96","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay"],"prefix":"10.1016","volume":"141","author":[{"given":"Jun","family":"Sakai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0787-0397","authenticated-orcid":false,"given":"Takuya","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norihito","family":"Tarumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhisa","family":"Misawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinsuke","family":"Tamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Imai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Iwata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigefumi","family":"Maesaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2017.08.009_bb0005","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1128\/jcm.34.5.1180-1183.1996","article-title":"Performance of Meridian ImmunoCard Mycoplasma test in a multicenter clinical trial","volume":"34","author":"Alexander","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0010","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1111\/j.1574-6976.2008.00129.x","article-title":"Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections","volume":"32","author":"Atkinson","year":"2008","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.mimet.2017.08.009_bb0015","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.jmii.2013.03.015","article-title":"Comparison of real-time polymerase chain reaction and serological tests for the confirmation of Mycoplasma pneumoniae infection in children with clinical diagnosis of atypical pneumonia","volume":"47","author":"Chang","year":"2014","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.mimet.2017.08.009_bb0020","first-page":"L220","article-title":"Induction of IL-8 by Mycoplasma pneumoniae membrane in BEAS-2B cells","volume":"295","author":"Chmura","year":"2008","journal-title":"Am. J. Phys. Lung Cell. Mol. Phys."},{"key":"10.1016\/j.mimet.2017.08.009_bb0025","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3343\/alm.2012.32.4.257","article-title":"Evaluation of peptide nucleic acid probe-based real-time PCR for detection of mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens","volume":"32","author":"Choi","year":"2011","journal-title":"Ann. Lab. Med."},{"key":"10.1016\/j.mimet.2017.08.009_bb0030","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1046\/j.1469-0691.2003.00590.x","article-title":"Laboratory diagnosis of Mycoplasma pneumoniae infection","volume":"9","author":"Daxboeck","year":"2003","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2017.08.009_bb0035","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1469-0691.2009.02968.x","article-title":"Occurrence of macrolide-resistant Mycoplasma pneumoniae strains in Germany","volume":"16","author":"Dumke","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"issue":"9","key":"10.1016\/j.mimet.2017.08.009_bb0040","doi-asserted-by":"crossref","DOI":"10.3201\/eid1909.121466","article-title":"Macrolide-resistant Mycoplasma pneumoniae in humans, Ontario, Canada, 2010\u20132011","volume":"19","author":"Eshaghi","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2017.08.009_bb0045","doi-asserted-by":"crossref","first-page":"603","DOI":"10.7754\/Clin.Lab.2014.141016","article-title":"Sensitivity and specificity of a loop-mediated isothermal amplification assay for the detection of Mycoplasma pneumoniae from nasopharyngeal swab samples compared with those of real-time PCR","volume":"61","author":"Ishiguro","year":"2015","journal-title":"Clin. Lab."},{"key":"10.1016\/j.mimet.2017.08.009_bb0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0151654","article-title":"Novel methodology for rapid detection of KRAS mutation using PNA-LNA mediated loop-mediated isothermal amplification","volume":"11","author":"Itonaga","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2017.08.009_bb0055","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jiac.2013.09.009","article-title":"Clinical features, risk factors and treatment of fulminant Mycoplasma pneumoniae pneumonia: a review of the Japanese literature","volume":"20","author":"Izumikawa","year":"2014","journal-title":"J. Infect. Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0060","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1186\/s12879-015-1041-6","article-title":"Management of Mycoplasma genitalium infections - can we hit a moving target?","volume":"15","author":"Jensen","year":"2015","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.mimet.2017.08.009_bb0065","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1111\/jdv.13849","article-title":"2016 European guideline on Mycoplasma genitalium infections","volume":"30","author":"Jensen","year":"2016","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0070","doi-asserted-by":"crossref","first-page":"4046","DOI":"10.1128\/AAC.00663-13","article-title":"Nationwide surveillance of macrolide-resistant Mycoplasma pneumoniae infection in pediatric patients","volume":"57","author":"Kawai","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0075","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0133759","article-title":"A simple and rapid identification method for Mycobacterium bovis BCG with loop-mediated isothermal amplification","volume":"10","author":"Kouzaki","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2017.08.009_bb0080","doi-asserted-by":"crossref","first-page":"2770","DOI":"10.1128\/AAC.39.12.2770","article-title":"Transition mutations in the 23S rRNA of erythromycin-resistant isolates of Mycoplasma pneumoniae","volume":"39","author":"Lucier","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0085","doi-asserted-by":"crossref","first-page":"4624","DOI":"10.1128\/AAC.48.12.4624-4630.2004","article-title":"Characterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan","volume":"48","author":"Matsuoka","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0090","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/1471-2334-12-126","article-title":"Macrolide-resistant Mycoplasma pneumoniae in adolescents with community-acquired pneumonia","volume":"12","author":"Miyashita","year":"2012","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.mimet.2017.08.009_bb0095","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.jiac.2015.02.007","article-title":"Diagnostic sensitivity of a rapid antigen test for the detection of Mycoplasma pneumoniae: comparison with real-time PCR","volume":"21","author":"Miyashita","year":"2015","journal-title":"J. Infect. Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0100","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1128\/AAC.49.6.2302-2306.2005","article-title":"Emergence of macrolides-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation","volume":"49","author":"Morozumi","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0105","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/AAC.00779-07","article-title":"Increased macrolide resistance of Mycoplasma pneumoniae in pediatric patients with community-acquired pneumonia","volume":"52","author":"Morozumi","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0110","doi-asserted-by":"crossref","first-page":"7276","DOI":"10.1158\/0008-5472.CAN-05-0331","article-title":"Genetic heterogeneity of the epidermal growth factor receptor in non-small cell lung cancer cell lines revealed by a rapid and sensitive detection system, the peptide nucleic acid-locked nucleic acid PCR clamp","volume":"65","author":"Nagai","year":"2005","journal-title":"Cancer Res."},{"key":"10.1016\/j.mimet.2017.08.009_bb0115","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1126\/science.1962210","article-title":"Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide","volume":"254","author":"Nielsen","year":"1991","journal-title":"Science"},{"key":"10.1016\/j.mimet.2017.08.009_bb0120","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2017.08.009_bb0125","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/j.1348-0421.2001.tb01293.x","article-title":"Characteristics of macrolide-resistant Mycoplasma pneumoniae strains isolated from patients and induced with erythromycin in vitro","volume":"45","author":"Okazaki","year":"2001","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0130","doi-asserted-by":"crossref","first-page":"5332","DOI":"10.1093\/nar\/21.23.5332","article-title":"Single base pair mutation analysis by PNA directed PCR clamping","volume":"21","author":"Orum","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2017.08.009_bb0135","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1128\/JCM.01431-15","article-title":"Isothermal detection of Mycoplasma pneumoniae directly from respiratory clinical specimens","volume":"53","author":"Petrone","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0140","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1099\/jmm.0.46071-0","article-title":"Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae","volume":"54","author":"Saito","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0145","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1128\/AAC.50.2.709-712.2006","article-title":"Clinical evaluation of macrolide-resistant Mycoplasma pneumoniae","volume":"50","author":"Suzuki","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0150","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1097\/INF.0b013e31827aa7bd","article-title":"Community outbreak of macrolide-resistant Mycoplasma pneumoniae in Yamagata, Japan in 2009","volume":"32","author":"Suzuki","year":"2013","journal-title":"Pediatr. Infect. Dis."},{"key":"10.1016\/j.mimet.2017.08.009_bb0155","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1128\/CMR.00006-11","article-title":"Mycoplasma genitalium: from Chrysalis to multicolored butterfly","volume":"24","author":"Taylor-Robinson","year":"2011","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.mimet.2017.08.009_bb0160","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1093\/nar\/24.5.983","article-title":"Simple and sensitive detection of mutations in the ras proto-oncogenes using PNA-mediated PCR clamping","volume":"24","author":"Thiede","year":"1996","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2017.08.009_bb0165","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1099\/jmm.0.47216-0","article-title":"Development of a loop-mediated isothermal amplification method for diagnosing Pneumocystis pneumonia","volume":"57","author":"Uemura","year":"2008","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/AAC.45.1.1-12.2001","article-title":"Macrolide resistance conferred by base substitutions in 23S rRNA","volume":"45","author":"Vester","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0175","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1128\/AAC.39.3.577","article-title":"Erythromycin resistance by ribosome modification","volume":"39","author":"Weisblum","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2017.08.009_bb0180","doi-asserted-by":"crossref","first-page":"693","DOI":"10.3389\/fmicb.2016.00693","article-title":"Epidemiology of Mycoplasma pneumoniae infections in Japan and therapeutic strategies for macrolide-resistant M. pneumoniae","volume":"7","author":"Yamazaki","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2017.08.009_bb0185","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.cytogfr.2004.01.001","article-title":"Cytokines in Mycoplasma pneumoniae infections","volume":"15","author":"Yang","year":"2004","journal-title":"Cytokine Growth Factor Rev."},{"key":"10.1016\/j.mimet.2017.08.009_bb0190","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1128\/AAC.02060-12","article-title":"Surveillance of macrolide-resistant Mycoplasma pneumoniae in Beijing, China, from 2008 to 2012","volume":"57","author":"Zhao","year":"2013","journal-title":"Antimicrob. Agents Chemother."}],"updated-by":[{"DOI":"10.1016\/j.mimet.2017.11.004","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000}}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701217302178?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701217302178?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:55:54Z","timestamp":1759103754000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701217302178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":38,"alternative-id":["S0167701217302178"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2017.08.009","relation":{"erratum":[{"id-type":"doi","id":"10.1016\/j.mimet.2017.11.004","asserted-by":"object"}]},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2017,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2017.08.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}