{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T02:54:41Z","timestamp":1785293681557,"version":"3.55.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004726","name":"Ministry of Health","doi-asserted-by":"publisher","award":["RCIZSUM 14\/2014"],"award-info":[{"award-number":["RCIZSUM 14\/2014"]}],"id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2018,5]]},"DOI":"10.1016\/j.mimet.2018.03.009","type":"journal-article","created":{"date-parts":[[2018,3,21]],"date-time":"2018-03-21T19:32:36Z","timestamp":1521660756000},"page":"12-17","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants"],"prefix":"10.1016","volume":"148","author":[{"given":"Carla","family":"Sebastiani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovica","family":"Curcio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella","family":"Ciullo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Cruciani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Crotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Pesca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Torricelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Sebastianelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Felici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Biagetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2018.03.009_bb0005","series-title":"Practical Statistics for Medical Research","first-page":"406","article-title":"Comparing groups - continuous data","author":"Altman","year":"1991"},{"key":"10.1016\/j.mimet.2018.03.009_bb0010","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.theriogenology.2007.04.001","article-title":"Infectious causes of bovine abortion during mid-to late-gestation","volume":"68","author":"Anderson","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.mimet.2018.03.009_bb0015","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily","year":"1992","journal-title":"J. Trop. Med. Hyg."},{"key":"10.1016\/j.mimet.2018.03.009_bb0020","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0378-1135(99)00178-9","article-title":"The detection of Coxiella burnetii from ovine genital swabs,milk and fecal samples by the use of a single touchdown polymerase chain reaction","volume":"72","author":"Berri","year":"2000","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2018.03.009_bb0025","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/1471-2180-9-130","article-title":"Simultaneous differential detection of Chlamydophila abortus, Chlamydophila pecorum and Coxiella burnetii from aborted ruminant's clinical samples using multiplex PCR","volume":"9","author":"Berri","year":"2009","journal-title":"BMC Microbiol."},{"issue":"2","key":"10.1016\/j.mimet.2018.03.009_bb0030","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.femsle.2005.01.007","article-title":"Polimerase chain reaction assay specific for pathogenic leptospira based on the gene hap1 encoding the hemolysis-associated protein1","volume":"243","author":"Branger","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2018.03.009_bb0035","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.tifs.2014.03.008","article-title":"Guidelines for validation of qualitative qPCR methods","volume":"37","author":"Broeders","year":"2014","journal-title":"Trends Food Sci. Technol."},{"key":"10.1016\/j.mimet.2018.03.009_bb0040","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative QPCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mimet.2018.03.009_bb0045","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.theriogenology.2015.11.001","article-title":"Evaluation of bovine abortion cases and tissue suitability for identification of infectious agents in California diagnostic laboratory cases from 2007 to 2012","volume":"85","author":"Clothier","year":"2016","journal-title":"Theriogenology"},{"key":"10.1016\/j.mimet.2018.03.009_bb0050","first-page":"148","article-title":"Untersuchungen zur Rolle des Rotfuches (Vulpes vulpes) als moglicher endwirt von Neospora caninum","volume":"124","author":"Constantin","year":"2011","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"10.1016\/j.mimet.2018.03.009_bb0055","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.mcp.2005.09.003","article-title":"Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies","volume":"20","author":"Ehricht","year":"2006","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.mimet.2018.03.009_bb0060","series-title":"Verification of Analytical Methods for GMO Testing when Implementing Interlaboratory Validated Methods. EUR \u2013 Scientific and Technical Research Series","author":"EUR 24790","year":"2011"},{"key":"10.1016\/j.mimet.2018.03.009_bb0065","series-title":"La Brucellosi in Italia dal 1998 tal 2011. Rapporti ISTISAN 13\/45","author":"Graziani","year":"2013"},{"key":"10.1016\/j.mimet.2018.03.009_bb0070","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1177\/1040638713484729","article-title":"A prospective study of sheep and goat abortion using real-time polymerase chain reaction and cut point estimation shows Coxiella burnetii and Chlamydophila abortus infection concurrently with other major pathogens","volume":"25","author":"Hazlett","year":"2013","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2018.03.009_bb0075","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.mimet.2008.07.002","article-title":"Novel identification and differentiation of Brucella melitensis. B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real time PCR systems","volume":"25","author":"Hinic","year":"2008","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2018.03.009_bb0080","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.mcp.2009.01.004","article-title":"Real time PCR for the early detection and quantification of Coxiella burnetii as an alternative to the murine assay","volume":"23","author":"Howe","year":"2009","journal-title":"Mol. Cell. Probes"},{"issue":"9","key":"10.1016\/j.mimet.2018.03.009_bb0085","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.1128\/AEM.02823-06","article-title":"Optimization of 12 hour TaqMan PCR based method for detection of Salmonella bacteria in meat","volume":"73","author":"Josefsen","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2018.03.009_bb0090","first-page":"13","article-title":"Seroprevalence study of the main causes of abortion in dairy cattle in Morocco","volume":"52","author":"Lucchese","year":"2016","journal-title":"Vet. Ital."},{"issue":"Suppl","key":"10.1016\/j.mimet.2018.03.009_bb0095","first-page":"S15","article-title":"Neosporosi bovina in Italia: un biennio di attivita diagnostica","author":"Magnino","year":"2000","journal-title":"La Selezione Veterinaria"},{"key":"10.1016\/j.mimet.2018.03.009_bb0100","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.cvfa.2010.10.011","article-title":"Control of important causes of infectious abortion in sheep and goats","volume":"27","author":"Menzies","year":"2011","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.mimet.2018.03.009_bb0105","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1136\/vr.165.6.175","article-title":"Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin-embedded samples","volume":"165","author":"Navarro","year":"2009","journal-title":"Vet. Rec."},{"key":"10.1016\/j.mimet.2018.03.009_bb0110","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0890-8508(92)90002-F","article-title":"Amplification on an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella","volume":"6","author":"Rahn","year":"1992","journal-title":"Mol. Cell. Probes"},{"issue":"5","key":"10.1016\/j.mimet.2018.03.009_bb0115","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1177\/1040638713497483","article-title":"Simultaneous detection of Chlamydia spp., Coxiella burnetii, and Neospora caninum in abortion material of ruminants by multiplex real\u2013time polymerase chain reaction","volume":"25","author":"Reisenberg","year":"2013","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2018.03.009_bb0120","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1439-0442.2007.00913.x","article-title":"Aetiology of bovine abortion in Switzerland from 1986 to 1995 - a retrospective study with emphasis on detection of Neospora caninum and Toxoplasma gondii by PCR","volume":"54","author":"Reitt","year":"2007","journal-title":"J. Vet. Med. A Physiol. Pathol. Clin. Med."},{"key":"10.1016\/j.mimet.2018.03.009_bb0125","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: a re-emerging zoonosis","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet. Microbiol."},{"issue":"4","key":"10.1016\/j.mimet.2018.03.009_bb0130","first-page":"269","article-title":"Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus","volume":"50","author":"Selim","year":"2014","journal-title":"Vet. Ital."},{"key":"10.1016\/j.mimet.2018.03.009_bb0135","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the Lip 32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"4","key":"10.1016\/j.mimet.2018.03.009_bb0140","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1177\/1040638711407880","article-title":"Development of a set of multiplex standard polymerase chain reaction assay for the identification of infectious agents from aborted bovine clinical samples","volume":"23","author":"Tramuta","year":"2011","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2018.03.009_bb0145","series-title":"Proceedings of the 5th Meeting of the European Society for Chlamydia Research. Budapest, Hungary, September 1\u20134, 2004","article-title":"A PCR-RFLP assay targeting the 16S rRNA gene for the diagnosis of animal chlamydioses","author":"Vicari","year":"2004"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701218302069?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701218302069?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:55:43Z","timestamp":1759103743000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701218302069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":29,"alternative-id":["S0167701218302069"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2018.03.009","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2018,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2018.03.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}