{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T08:48:00Z","timestamp":1769244480511,"version":"3.49.0"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Concejo Nacional de Investigaci\u00f3n en Ciencia y T\u00e9cnica"},{"name":"Facultad de Bioqu\u00edmica y Cs. Biol\u00f3gicas"},{"DOI":"10.13039\/501100005746","name":"Universidad Nacional del Litoral","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005746","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hospital Escuela Eva Per\u00f3n"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.mimet.2018.04.015","type":"journal-article","created":{"date-parts":[[2018,4,21]],"date-time":"2018-04-21T14:06:37Z","timestamp":1524319597000},"page":"9-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Semiquantitative Dot Blot with the GRA8 antigen to differentiate the stages of toxoplasmosis infection"],"prefix":"10.1016","volume":"149","author":[{"given":"Juan Gabriel","family":"Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Julia","family":"Vilari\u00f1o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2018.04.015_bb0005","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.20506\/rst.28.3.1950","article-title":"Dot blot para el diagn\u00f3stico de la cisticercosis porcina","volume":"28","author":"Agudelo-Fl\u00f3rez","year":"2009","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.mimet.2018.04.015_bb0010","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1128\/JCM.38.3.1144-1150.2000","article-title":"Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay","volume":"38","author":"Aubert","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0015","first-page":"67","article-title":"Expression and single-step purification of GRA8 antigen of Toxoplasma gondii in Escherichia coli","volume":"3","author":"Babaie","year":"2011","journal-title":"Avicenna J. Med. Biotech."},{"issue":"6","key":"10.1016\/j.mimet.2018.04.015_bb0020","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005670","article-title":"Point-of-care testing for Toxoplasma gondii IgG\/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries","author":"Begeman","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"6","key":"10.1016\/j.mimet.2018.04.015_bb0025","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.jped.2016.02.014","article-title":"Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis","author":"Capobiango","year":"2016","journal-title":"J. Pediatr."},{"key":"10.1016\/j.mimet.2018.04.015_bb0030","first-page":"105","article-title":"Confirmation of acute toxoplasmosis infection in pregnant women","volume":"1","author":"Costa","year":"2017","journal-title":"J. Med. Microbiol. Imm. Res."},{"key":"10.1016\/j.mimet.2018.04.015_bb0035","first-page":"246","article-title":"Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies","volume":"33","author":"Costa","year":"2016","journal-title":"Trop. Biomed."},{"key":"10.1016\/j.mimet.2018.04.015_bb0040","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.micpath.2016.09.021","article-title":"Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection","volume":"100","author":"Costa","year":"2016","journal-title":"Microbiol. Path."},{"key":"10.1016\/j.mimet.2018.04.015_bb0045","doi-asserted-by":"crossref","DOI":"10.2217\/bmm-2017-0345","article-title":"Antigens to detect the acute phase of toxoplasmosis in pregnant women: standardized comparison","volume":"12","author":"Costa","year":"2018","journal-title":"Biomark. Med"},{"key":"10.1016\/j.mimet.2018.04.015_bb0050","first-page":"249","article-title":"Efficient production of the Toxoplasma gondii GRA6, P35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis","volume":"50","author":"Hiszczyjska-Sawicka","year":"2005","journal-title":"Acta Parasitol."},{"issue":"7","key":"10.1016\/j.mimet.2018.04.015_bb0055","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1128\/JCM.00464-11","article-title":"Identification of Toxoplasma gondii SUB1 antigen as a marker for acute infection by use of an innovative evaluation method","author":"Hruzik","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0060","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1128\/JCM.42.1.351-353.2004","article-title":"Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats","volume":"42","author":"Huang","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0065","doi-asserted-by":"crossref","first-page":"493","DOI":"10.3347\/kjp.2014.52.5.493","article-title":"A new IgG immunoblot kit for diagnosis of toxoplasmosis in pregnant women","volume":"5","author":"Khammari","year":"2014","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0070","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1600-0463.2010.02629.x","article-title":"Recombinant proteins in the diagnosis of toxoplasmosis","volume":"118","author":"Kotresha","year":"2010","journal-title":"APMIS"},{"key":"10.1016\/j.mimet.2018.04.015_bb0075","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1128\/CDLI.7.5.781-787.2000","article-title":"Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzime-linked immunosorbent assay with a combination of recombinant antigens","volume":"7","author":"Li","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0080","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1128\/JCM.38.1.179-184.2000","article-title":"Serodiagnosis of recently acquired Toxoplasma gondii infection with a recombinant antigen","volume":"38","author":"Li","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0085","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.exppara.2005.12.014","article-title":"Toxoplasma gondii: expression pattern and detection of infection using full-length recombinant P35 antigen","volume":"113","author":"Lu","year":"2006","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0090","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0022-1759(00)00241-6","article-title":"A multi-antigen print immunoassay for the development of serological diagnosis of infectious diseases","volume":"242","author":"Lyashchenko","year":"2000","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.mimet.2018.04.015_bb0095","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0001-2998(78)80014-2","article-title":"Basic principles of ROC analysis","volume":"8","author":"Metz","year":"1978","journal-title":"Semin. Nucl. Med."},{"key":"10.1016\/j.mimet.2018.04.015_bb0100","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0022-1759(83)90399-X","article-title":"Dot enzyme-linked immunosorbent assay (DOT-ELISA) a microtechnique for the rapid diagnosis of visceral Leishmaniasis","volume":"64","author":"Pappas","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.mimet.2018.04.015_bb0105","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1645\/GE-2712.1","article-title":"A review: competence, compromise, and concomitance-reaction of the host cell to Toxoplasma gondii infection and development","volume":"97","author":"Peng","year":"2011","journal-title":"J. Parasitol."},{"key":"10.1016\/j.mimet.2018.04.015_bb0110","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Robert-Gangneux","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.mimet.2018.04.015_bb0115","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","article-title":"Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa","volume":"166","author":"Sch\u00e4gger","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mimet.2018.04.015_bb0120","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jviromet.2011.01.011","article-title":"Simultaneous and rapid detection of white spot syndrome virus and yellow head virus infection in shrimp with a dual immunochromatographic strip test","volume":"173","author":"Sithigorngul","year":"2011","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mimet.2018.04.015_bb0125","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1080\/20477724.2016.1238186","article-title":"Identification, production and assessment of two Toxoplasma gondii recombinant proteins for use in a toxoplasma IgG avidity assay","volume":"8","author":"Teh","year":"2016","journal-title":"Pathog. Glob. Health."},{"key":"10.1016\/j.mimet.2018.04.015_bb0130","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1080\/00365549850160963","article-title":"Pyrimethamine-sulfadoxine treatment of congenital toxoplasmosis: follow-up of 78 cases between 1980 and 1997","volume":"30","author":"Villena","year":"1998","journal-title":"Reims Toxoplasmosis Group. Scand. J. Infect. Dis."},{"key":"10.1016\/j.mimet.2018.04.015_bb0135","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.eimc.2013.05.006","article-title":"Estandarizaci\u00f3n de la t\u00e9cnica Dot-ELISA para la detecci\u00f3n de anticuerpos anti-Trypanosoma cruzi y su comparaci\u00f3n con ELISA y Western blot","volume":"32","author":"Yunuen Cervantes-Land\u00edna","year":"2014","journal-title":"Enferm. Infecc. Microbiol. Clin."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701218301830?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701218301830?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:56:31Z","timestamp":1759103791000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701218301830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":27,"alternative-id":["S0167701218301830"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2018.04.015","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Semiquantitative Dot Blot with the GRA8 antigen to differentiate the stages of toxoplasmosis infection","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2018.04.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}