{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T17:17:56Z","timestamp":1775841476554,"version":"3.50.1"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100008238","name":"Bureau of Science and Technology of Hebei Province","doi-asserted-by":"publisher","award":["16226604D"],"award-info":[{"award-number":["16226604D"]}],"id":[{"id":"10.13039\/501100008238","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008238","name":"Bureau of Science and Technology of Hebei Province","doi-asserted-by":"publisher","award":["SLRC2017039"],"award-info":[{"award-number":["SLRC2017039"]}],"id":[{"id":"10.13039\/501100008238","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["C2017325001"],"award-info":[{"award-number":["C2017325001"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1016\/j.mimet.2019.02.015","type":"journal-article","created":{"date-parts":[[2019,2,25]],"date-time":"2019-02-25T01:20:27Z","timestamp":1551057627000},"page":"56-61","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["Rapid and sensitive detection of Mycoplasma hyopneumoniae by recombinase polymerase amplification assay"],"prefix":"10.1016","volume":"159","author":[{"given":"Libing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiwen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoxi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinfeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingan","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianchang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanzhe","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2019.02.015_bb0005","doi-asserted-by":"crossref","first-page":"e71642","DOI":"10.1371\/journal.pone.0071642","article-title":"A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus","volume":"8","author":"Abd El Wahed","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2019.02.015_bb0010","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jviromet.2013.06.027","article-title":"A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay","volume":"193","author":"Amer","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mimet.2019.02.015_bb0015","first-page":"654","article-title":"Cross-reactions between Mycoplasma hyopneumoniae and Mycoplasma flocculare--practical implications for the serodiagnosis of mycoplasmal pneumonia of swine","volume":"23","author":"Armstrong","year":"1987","journal-title":"Isr. J. Med. Sci."},{"key":"10.1016\/j.mimet.2019.02.015_bb0020","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00135-13","article-title":"Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification","volume":"4","author":"Boyle","year":"2013","journal-title":"MBio."},{"key":"10.1016\/j.mimet.2019.02.015_bb0025","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1177\/104063870701900115","article-title":"Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples","volume":"19","author":"Cai","year":"2007","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2019.02.015_bb0030","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1177\/104063879901100307","article-title":"Application of a nested polymerase chain reaction assay to detect Mycoplasma hyopneumoniae from nasal swabs","volume":"11","author":"Calsamiglia","year":"1999","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2019.02.015_bb0035","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.mcp.2014.11.005","article-title":"Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology","volume":"29","author":"Daher","year":"2015","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.mimet.2019.02.015_bb0040","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1373\/clinchem.2015.245829","article-title":"Recombinase polymerase amplification for diagnostic applications","volume":"62","author":"Daher","year":"2016","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mimet.2019.02.015_bb0045","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.vetmic.2006.04.022","article-title":"Variable number of tandem aminoacid repeats in adhesion-related CDS products in Mycoplasma hyopneumoniae strains","volume":"116","author":"de Castro","year":"2006","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0050","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.vetmic.2004.05.007","article-title":"Development of two real-time PCR assays for the detection of Mycoplasma hyopneumoniae in clinical samples","volume":"102","author":"Dubosson","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0055","article-title":"Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015","volume":"20","author":"Faye","year":"2015","journal-title":"Euro Surv.: Bull. Eur. sur les maladies transmissibles = Eur. Commun. Dis. Bull."},{"key":"10.1016\/j.mimet.2019.02.015_bb0060","first-page":"202","article-title":"Serological cross-reactivity of porcine reference antisera to Mycoplasma hyopneumoniae, M. flocculare, M. hyorhinis and M. hyosynoviae indicated by the enzyme-linked immunosorbent assay, complement fixation and indirect hemagglutination tests","volume":"48","author":"Freeman","year":"1984","journal-title":"Can. J. Comp. Med."},{"key":"10.1016\/j.mimet.2019.02.015_bb0065","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1177\/104063870201400603","article-title":"Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine","volume":"14","author":"Kurth","year":"2002","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.mimet.2019.02.015_bb0070","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1007\/s11274-012-1216-x","article-title":"Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae","volume":"29","author":"Li","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0075","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.mcp.2016.01.009","article-title":"Factors influencing recombinase polymerase amplification (RPA) assay outcomes at point of care","volume":"30","author":"Lillis","year":"2016","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.mimet.2019.02.015_bb0080","doi-asserted-by":"crossref","first-page":"4677","DOI":"10.4238\/2015.May.4.27","article-title":"A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene","volume":"14","author":"Liu","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"10.1016\/j.mimet.2019.02.015_bb0085","doi-asserted-by":"crossref","first-page":"7977","DOI":"10.1128\/JB.01068-07","article-title":"Array-based genomic comparative hybridization analysis of field strains of Mycoplasma hyopneumoniae","volume":"189","author":"Madsen","year":"2007","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0090","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1051\/vetres:2007006","article-title":"Diversity of Mycoplasma hyopneumoniae in pig farms revealed by direct molecular typing of clinical material","volume":"38","author":"Mayor","year":"2007","journal-title":"Vet. Res."},{"key":"10.1016\/j.mimet.2019.02.015_bb0095","doi-asserted-by":"crossref","first-page":"40244","DOI":"10.1038\/srep40244","article-title":"Development of a recombinase polymerase amplification assay for detection of epidemic human noroviruses","volume":"7","author":"Moore","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mimet.2019.02.015_bb0100","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.vetmic.2004.11.006","article-title":"Serological diagnosis of enzootic pneumonia of swine by a double-sandwich enzyme-linked immunosorbent assay using a monoclonal antibody and recombinant antigen (P46) of Mycoplasma hyopneumoniae","volume":"105","author":"Okada","year":"2005","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0105","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0110","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.tvjl.2008.02.020","article-title":"Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection","volume":"181","author":"Sibila","year":"2009","journal-title":"Vet. J."},{"key":"10.1016\/j.mimet.2019.02.015_bb0115","doi-asserted-by":"crossref","first-page":"593","DOI":"10.20506\/rst.12.2.699","article-title":"Application of enzyme-linked immunosorbent assay for the surveillance of Mycoplasma hyopneumoniae infection in pigs","volume":"12","author":"Sorensen","year":"1993","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.mimet.2019.02.015_bb0120","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.mimet.2005.12.002","article-title":"Comparison of molecular techniques for the typing of Mycoplasma hyopneumoniae isolates","volume":"66","author":"Stakenborg","year":"2006","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2019.02.015_bb0125","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/AEM.64.2.543-548.1998","article-title":"Detection of Mycoplasma hyopneumoniae by air sampling with a nested PCR assay","volume":"64","author":"Stark","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0130","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1128\/JCM.02366-07","article-title":"Real-time PCR assays to address genetic diversity among strains of Mycoplasma hyopneumoniae","volume":"46","author":"Strait","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0135","series-title":"Diseases of Swine","first-page":"779","article-title":"Mycoplasmosis","author":"Thacker","year":"2012"},{"key":"10.1016\/j.mimet.2019.02.015_bb0140","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0378-1135(00)00228-5","article-title":"A nested PCR assay for the detection of Mycoplasma hyopneumoniae in tracheobronchiolar washings from pigs","volume":"76","author":"Verdin","year":"2000","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2019.02.015_bb0145","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jviromet.2017.01.017","article-title":"Reverse transcription recombinase polymerase amplification assay for the rapid detection of type 2 porcine reproductive and respiratory syndrome virus","volume":"243","author":"Wang","year":"2017","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mimet.2019.02.015_bb0150","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.ab.2017.12.012","article-title":"Real-time RPA assay for rapid detection and differentiation of wild-type pseudorabies and gE-deleted vaccine viruses","volume":"543","author":"Wang","year":"2018","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mimet.2019.02.015_bb0155","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12985-017-0688-6","article-title":"Development of real-time and lateral flow strip reverse transcription recombinase polymerase amplification assays for rapid detection of peste des petits ruminants virus","volume":"14","author":"Yang","year":"2017","journal-title":"Virol. J."},{"key":"10.1016\/j.mimet.2019.02.015_bb0160","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s12985-017-0792-7","article-title":"Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus","volume":"14","author":"Yang","year":"2017","journal-title":"Virol. J."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701219300028?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701219300028?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:58:26Z","timestamp":1759103906000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701219300028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":32,"alternative-id":["S0167701219300028"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2019.02.015","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid and sensitive detection of Mycoplasma hyopneumoniae by recombinase polymerase amplification assay","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2019.02.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}