{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:06:38Z","timestamp":1789563998570,"version":"build-2803163510"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"am","delay-in-days":354,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007579","name":"Auburn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007579","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009168","name":"Animal and Plant Health Inspection Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009168","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1016\/j.mimet.2020.105892","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T11:31:54Z","timestamp":1584185514000},"page":"105892","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Validation of a screening method for the detection of colistin-resistant E. coli containing mcr-1 in feral swine feces"],"prefix":"10.1016","volume":"172","author":[{"given":"Jeffrey C.","family":"Chandler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan B.","family":"Franklin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah N.","family":"Bevins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin T.","family":"Bentler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Bonnedahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Ahlstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bledar","family":"Bisha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan A.","family":"Shriner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.mimet.2020.105892_bb0005","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1111\/1758-2229.12779","article-title":"Early emergence of mcr-1-positive Enterobacteriaceae in gulls from Spain and Portugal","volume":"11","author":"Ahlstrom","year":"2019","journal-title":"Environ. Microbiol. Rep."},{"key":"10.1016\/j.mimet.2020.105892_bb0010","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.3389\/fmicb.2018.01217","article-title":"Molecular epidemiology of mcr-encoded colistin resistance in enterobacteriaceae from food-producing animals in Italy revealed through the EU harmonized antimicrobial resistance monitoring","volume":"9","author":"Alba","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0015","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1080\/03079457.2018.1524573","article-title":"A review on the current situation and challenges of colistin resistance in poultry production","volume":"47","author":"Apostolakos","year":"2018","journal-title":"Avian Pathol."},{"key":"10.1016\/j.mimet.2020.105892_bb0020","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1089\/mdr.2017.0026","article-title":"First report of the plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli ST405 isolated from wildlife in Bejaia, Algeria","volume":"24","author":"Bachiri","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"10.1016\/j.mimet.2020.105892_bb0030","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1093\/biosci\/biu015","article-title":"Consequences associated with the recent range expansion of nonnative feral swine","volume":"64","author":"Bevins","year":"2014","journal-title":"BioScience."},{"key":"10.1016\/j.mimet.2020.105892_bb0035","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1586\/eri.12.78","article-title":"Colistin: an update on the antibiotic of the 21st century","volume":"10","author":"Biswas","year":"2012","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"10.1016\/j.mimet.2020.105892_bb0040","doi-asserted-by":"crossref","first-page":"2318","DOI":"10.1093\/jac\/dkw139","article-title":"Real-time PCR for detection of plasmid-mediated polymyxin resistance (mcr-1) from cultured bacteria and stools","volume":"71","author":"Bontron","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0045","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.tifs.2014.03.008","article-title":"Guidelines for validation of qualitative real-time PCR methods","volume":"37","author":"Broeders","year":"2014","journal-title":"Trends Food Sci. Technol."},{"key":"10.1016\/j.mimet.2020.105892_bb0050","series-title":"Model Selection and Multimodel Inference: A Practical Information-Theoretic Approach","author":"Burnham","year":"2002"},{"key":"10.1016\/j.mimet.2020.105892_bb0055","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s10096-016-2846-y","article-title":"MCR: modern colistin resistance","volume":"36","author":"Caniaux","year":"2017","journal-title":"Eur. J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0060","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00853-19","article-title":"Identification of novel mobilized colistin resistance gene mcr-9 in a multidrug-resistant, colistin-susceptible Salmonella enterica serotype Typhimurium isolate","volume":"10","author":"Carroll","year":"2019","journal-title":"MBio."},{"key":"10.1016\/j.mimet.2020.105892_bb0065","first-page":"312","article-title":"A method to detect Escherichia coli carrying the colistin-resistance genes mcr-1 and mcr-2 using a single real-time polymerase chain reaction and its application to chicken cecal and porcine fecal samples","volume":"82","author":"Chalmers","year":"2018","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.mimet.2020.105892_bb0070","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.1128\/JCM.00268-17","article-title":"Colistin and polymyxin B susceptibility testing for carbapenem-resistant and mcr-positive Enterobacteriaceae: comparison of Sensititre, MicroScan, Vitek 2, and Etest with broth microdilution","volume":"55","author":"Chew","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0075","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ijfoodmicro.2019.02.006","article-title":"Revealing mcr-1-positive ESBL-producing Escherichia coli strains among Enterobacteriaceae from food-producing animals (bovine, swine and poultry) and meat (bovine and swine), Portugal, 2010-2015","volume":"296","author":"Clemente","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0080","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jgar.2017.01.007","article-title":"A SYBR\u00ae green-based real-time PCR method for improved detection of mcr-1-mediated colistin resistance in human stool samples","volume":"9","author":"Dona","year":"2017","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"10.1016\/j.mimet.2020.105892_bb0085","first-page":"3359","article-title":"Rapid detection and discrimination of chromosome-and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test","volume":"73","author":"Dortet","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0090","series-title":"Recommendations for MIC Determination of Colistin (polymyxin E) as Recommended by the Joint CLSI-EUCAST Polymyxin Breakpoints Working Group","author":"European Committee on Antimicrobial Susceptibility Testing (EUCAST)","year":"2016"},{"key":"10.1016\/j.mimet.2020.105892_bb0095","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1469-0691.2007.01912.x","article-title":"Heteroresistance: a concern of increasing clinical significance?","volume":"14","author":"Falagas","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2020.105892_bb0100","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00234-17","article-title":"Colistin-resistant mcr-1-positive Escherichia coli on public beaches, an infectious threat emerging in recreational waters","volume":"61","author":"Fernandes","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0105","doi-asserted-by":"crossref","first-page":"4408","DOI":"10.1038\/s41598-020-61318-2","article-title":"Gulls as sources of environmental contamination by colistin-resistant bacteria","volume":"10","author":"Franklin","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mimet.2020.105892_bb0110","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/zph.12147","article-title":"A scoping review of the role of wildlife in the transmission of bacterial pathogens and antimicrobial resistance to the food chain","volume":"62","author":"Greig","year":"2015","journal-title":"Zoonoses Public Health"},{"key":"10.1016\/j.mimet.2020.105892_bb0115","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0159863","article-title":"Prevalence of mcr-1 in E. coli from livestock and food in Germany, 2010-2015","volume":"11","author":"Irrgang","year":"2016","journal-title":"PLoS ONE"},{"key":"10.1016\/j.mimet.2020.105892_bb0120","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1128\/JCM.44.3.872-875.2006","article-title":"Sensitivity of Escherichia coli O157 detection in bovine feces assessed by broth enrichment followed by immunomagnetic separation and direct plating methodologies","volume":"44","author":"LeJeune","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0125","article-title":"A multiplex SYBR green real-time PCR assay for the detection of three colistin resistance genes from cultured bacteria, feces, and environment samples","volume":"8","author":"Li","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0130","doi-asserted-by":"crossref","first-page":"2335","DOI":"10.1093\/jac\/dkw262","article-title":"The colistin resistance mcr-1 gene is going wild","volume":"71","author":"Liakopoulos","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0135","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1592\/phco.30.12.1279","article-title":"Resurgence of colistin: a review of resistance, toxicity, pharmacodynamics, and dosing","volume":"30","author":"Lim","year":"2010","journal-title":"Pharmacotherapy."},{"key":"10.1016\/j.mimet.2020.105892_bb0140","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","article-title":"Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study","volume":"16","author":"Liu","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.mimet.2020.105892_bb0145","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1128\/AAC.01406-06","article-title":"Comparative evaluation of the VITEK 2, disk diffusion, etest, broth microdilution, and agar dilution susceptibility testing methods for colistin in clinical isolates, including heteroresistant Enterobacter cloacae and Acinetobacter baumannii strains","volume":"51","author":"Lo-Ten-Foe","year":"2007","journal-title":"Antimicrob. Agents Ch."},{"key":"10.1016\/j.mimet.2020.105892_bb0150","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.02244-16","article-title":"Prevalence of mcr-1 in the cecal contents of food animals in the United States","volume":"61","author":"Meinersmann","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0155","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.ijantimicag.2016.07.001","article-title":"First description of plasmid-mediated colistin-resistant extended-spectrum beta-lactamase-producing Escherichia coli in a wild migratory bird from Asia","volume":"48","author":"Mohsin","year":"2016","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.mimet.2020.105892_bb0160","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.vetmic.2018.06.006","article-title":"Dissemination of the mcr-1 colistin resistance gene among pigs: an experimental study","volume":"221","author":"Mourand","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0165","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1093\/jac\/dkw192","article-title":"Detection of the plasmid-mediated colistin-resistance gene mcr-1 in clinical isolates and stool specimens obtained from hospitalized patients using a newly developed real-time PCR assay","volume":"71","author":"Nijhuis","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0170","doi-asserted-by":"crossref","DOI":"10.1002\/mbo3.682","article-title":"Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods","volume":"8","author":"Osei Sekyere","year":"2019","journal-title":"Microbiologyopen."},{"key":"10.1016\/j.mimet.2020.105892_bb0175","series-title":"R Foundation for Statistical Computing, Vienna, Austria","article-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2017"},{"key":"10.1016\/j.mimet.2020.105892_bb0180","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3389\/fmicb.2014.00023","article-title":"Potential impact of antimicrobial resistance in wildlife, environment and human health","volume":"5","author":"Radhouani","year":"2014","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0185","doi-asserted-by":"crossref","first-page":"6881","DOI":"10.1128\/JB.00619-08","article-title":"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates","volume":"190","author":"Rasko","year":"2008","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2020.105892_bb0190","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.3389\/fmicb.2016.01789","article-title":"Colistin in pig production: chemistry, mechanism of antibacterial action, microbial resistance emergence, and one health perspectives","volume":"7","author":"Rhouma","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0195","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1093\/jac\/dkw245","article-title":"Detection of the mcr-1 gene in Escherichia coli prevalent in the migratory bird species Larus argentatus","volume":"71","author":"Ruzauskas","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.mimet.2020.105892_bb0200","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1111\/1365-2664.12866","article-title":"Interpreting and predicting the spread of invasive wild pigs","volume":"54","author":"Snow","year":"2017","journal-title":"J. Appl. Ecol."},{"key":"10.1016\/j.mimet.2020.105892_bb0205","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.tim.2018.02.006","article-title":"Towards understanding MCR-like colistin resistance","volume":"26","author":"Sun","year":"2018","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.mimet.2020.105892_bb0210","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/j.1469-0691.2007.01708.x","article-title":"Comparison of Etest, Vitek and agar dilution for susceptibility testing of colistin","volume":"13","author":"Tan","year":"2007","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2020.105892_bb0215","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/S1473-3099(16)30329-2","article-title":"China bans colistin as a feed additive for animals","volume":"16","author":"Walsh","year":"2016","journal-title":"Lancet Infectious Diseases"},{"key":"10.1016\/j.mimet.2020.105892_bb0220","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/S1473-3099(16)30527-8","article-title":"Prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive Enterobacteriaceae in patients and healthy adults from China: an epidemiological and clinical study","volume":"17","author":"Wang","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.mimet.2020.105892_bb0225","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1038\/s41467-018-03205-z","article-title":"The global distribution and spread of the mobilized colistin resistance gene mcr-1","volume":"9","author":"Wang","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mimet.2020.105892_bb0230","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1080\/14787210.2016.1216314","article-title":"On the path to untreatable infections: colistin use in agriculture and the end of \u2018last resort\u2019 antibiotics","volume":"14","author":"Watkins","year":"2016","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"10.1016\/j.mimet.2020.105892_bb0235","first-page":"21","article-title":"Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016","author":"Xavier","year":"2016","journal-title":"Eur. Surveill."},{"key":"10.1016\/j.mimet.2020.105892_bb0240","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.scitotenv.2019.05.251","article-title":"Swine waste: a reservoir of high-risk blaNDM and mcr-1","volume":"683","author":"Yang","year":"2019","journal-title":"Sci. Total Environ."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701220300713?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701220300713?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:08:58Z","timestamp":1762254538000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701220300713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":47,"alternative-id":["S0167701220300713"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2020.105892","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of a screening method for the detection of colistin-resistant E. coli containing mcr-1 in feral swine feces","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2020.105892","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"105892"}}