{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:17:11Z","timestamp":1791231431602,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["12I0317N"],"award-info":[{"award-number":["12I0317N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["11V4315N"],"award-info":[{"award-number":["11V4315N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014054","name":"Drugs for Neglected Diseases initiative","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","award":["TT-ZAPBOF 33049"],"award-info":[{"award-number":["TT-ZAPBOF 33049"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","award":["CM1307"],"award-info":[{"award-number":["CM1307"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006338","name":"Vlaamse Interuniversitaire Raad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.mimet.2020.105935","type":"journal-article","created":{"date-parts":[[2020,5,3]],"date-time":"2020-05-03T21:04:28Z","timestamp":1588539868000},"page":"105935","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Comparative evaluation of nucleic acid stabilizing reagents for RNA- and DNA-based Leishmania detection in blood as proxy for visceral burdens"],"prefix":"10.1016","volume":"173","author":[{"given":"Eline","family":"Eberhardt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rik","family":"Hendrickx","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Van den Kerkhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Severine","family":"Monnerat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Hendrickx","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Maes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Caljon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.mimet.2020.105935_bb0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0000599","article-title":"Of cattle, sand flies and men: a systematic review of risk factor analyses for south Asian visceral leishmaniasis and implications for elimination","volume":"4","author":"Bern","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"3","key":"10.1016\/j.mimet.2020.105935_bb0010","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/jcm.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acids","volume":"28","author":"Boom","year":"1990","journal-title":"J. Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.mimet.2020.105935_bb0015","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1128\/CDLI.8.4.828-831.2001","article-title":"Real-time PCR as a new tool for quantifying Leishmania infantum in liver in infected mice","volume":"8","author":"Bretagne","year":"2001","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.mimet.2020.105935_bb0020","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/S0169-4758(98)01330-1","article-title":"Kinetoplast DNA minicircle database","volume":"14","author":"Brewster","year":"1998","journal-title":"Parasitol. Today"},{"issue":"5","key":"10.1016\/j.mimet.2020.105935_bb0025","doi-asserted-by":"crossref","first-page":"970","DOI":"10.4269\/ajtmh.15-0145","article-title":"A novel molecular test to diagnose canine visceral Leishmaniasis at the point of care","volume":"93","author":"Castellanos-Gonzalez","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.mimet.2020.105935_bb0030","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.mcp.2016.08.006","article-title":"Leishmania-FAST15: a rapid, sensitive and low-cost real-time PCR assay for the detection of Leishmania infantum and Leishmania braziliensis kinetoplast DNA in canine blood samples","volume":"31","author":"Dantas-Torres","year":"2017","journal-title":"Mol. Cell. Probes"},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0035","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.pt.2005.11.004","article-title":"Risk factors in the spread of leishmaniases: towards integrated monitoring?","volume":"22","author":"Dujardin","year":"2006","journal-title":"Trends Parasitol."},{"issue":"2","key":"10.1016\/j.mimet.2020.105935_bb0040","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jmoldx.2017.12.003","article-title":"Evaluation of a pan-Leishmania spliced-leader RNA detection method in human blood and experimentally infected Syrian Golden hamsters","volume":"20","author":"Eberhardt","year":"2018","journal-title":"J. Mol. Diagn."},{"issue":"3\u20134","key":"10.1016\/j.mimet.2020.105935_bb0045","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetpar.2006.01.011","article-title":"Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis","volume":"137","author":"Francino","year":"2006","journal-title":"Vet. Parasitol."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0050","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s12639-014-0452-4","article-title":"SYBR green-based detection of Leishmania infantum DNA using peripheral blood samples","volume":"40","author":"Ghasemian","year":"2016","journal-title":"J. Parasit. Dis."},{"issue":"9","key":"10.1016\/j.mimet.2020.105935_bb0055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0185606","article-title":"Real-time PCR in detection and quantitation of Leishmania donovani for the diagnosis of visceral Leishmaniasis patients and the monitoring of their response to treatment","volume":"12","author":"Hossain","year":"2017","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.mimet.2020.105935_bb0060","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0167-7012(03)00025-3","article-title":"Molecular methods for the assessment of bacterial viability","volume":"53","author":"Keer","year":"2003","journal-title":"J. Microbiol. Methods"},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0065","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1128\/JCM.38.1.236-240.2000","article-title":"Optimized PCR using patient blood samples for diagnosis and follow-up of visceral Leishmaniasis, with special reference to AIDS patients","volume":"38","author":"Lachaud","year":"2000","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0070","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1128\/JCM.40.1.210-215.2002","article-title":"Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis","volume":"40","author":"Lachaud","year":"2002","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.mimet.2020.105935_bb0075","doi-asserted-by":"crossref","first-page":"6602","DOI":"10.1074\/jbc.274.10.6602","article-title":"Developmental regulation of spliced leader RNA gene in Leishmania donovani amastigotes is mediated by specific polyadenylation","volume":"274","author":"Lamontagne","year":"1999","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0080","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0036-46652014000100001","article-title":"Animal models for the study of leishmaniasis immunology","volume":"56","author":"Loria-Cervera","year":"2014","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"issue":"11","key":"10.1016\/j.mimet.2020.105935_bb0085","doi-asserted-by":"crossref","first-page":"5249","DOI":"10.1128\/JCM.42.11.5249-5255.2004","article-title":"Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity","volume":"42","author":"Mary","year":"2004","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0090","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/qjmed\/hct116","article-title":"Leishmaniasis: clinical syndromes and treatment","volume":"107","author":"McGwire","year":"2014","journal-title":"QJM"},{"issue":"18","key":"10.1016\/j.mimet.2020.105935_bb0095","doi-asserted-by":"crossref","first-page":"7341","DOI":"10.1093\/nar\/14.18.7341","article-title":"Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence","volume":"14","author":"Miller","year":"1986","journal-title":"Nucleic Acids Res."},{"issue":"5","key":"10.1016\/j.mimet.2020.105935_bb0100","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1128\/JCM.40.5.1666-1669.2002","article-title":"Real-time PCR for detection and quantitation of Leishmania in mouse tissues","volume":"40","author":"Nicolas","year":"2002","journal-title":"J. Clin. Microbiol."},{"issue":"12","key":"10.1016\/j.mimet.2020.105935_bb0105","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006052","article-title":"Drug resistance and treatment failure in leishmaniasis: a 21st century challenge","volume":"11","author":"Ponte-Sucre","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.mimet.2020.105935_bb0110","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2147\/CLEP.S44267","article-title":"Epidemiology of visceral leishmaniasis","volume":"6","author":"Ready","year":"2014","journal-title":"J. Clin. Epidemiol."},{"key":"10.1016\/j.mimet.2020.105935_bb0115","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s40249-016-0112-2","article-title":"Current challenges in treatment options for visceral leishmaniasis in India: a public health perspective","volume":"5","author":"Singh","year":"2016","journal-title":"Infect. Dis. Poverty."},{"issue":"6","key":"10.1016\/j.mimet.2020.105935_bb0120","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1007\/s00281-012-0350-8","article-title":"Immunopathogenesis of non-healing American cutaneous leishmaniasis and progressive visceral leishmaniasis","volume":"34","author":"Soong","year":"2012","journal-title":"Semin. Immunopathol."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0125","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1645\/GE-3221.1","article-title":"Real-time PCR to quantify Leishmania donovani in hamsters","volume":"99","author":"Srivastava","year":"2013","journal-title":"J. Parasitol."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0130","first-page":"80","article-title":"Host resistance to the Khartoum strain of Leishmania donovani","volume":"45","author":"Stauber","year":"1958","journal-title":"Rice Inst. Pam."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0135","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ijpddr.2013.11.001","article-title":"Duplex quantitative reverse-transcriptase PCR for simultaneous assessment of drug activity against Leishmania intracellular amastigotes and their host cells","volume":"4","author":"van den Bogaart","year":"2014","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0140","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.ijpddr.2018.01.006","article-title":"In vitro and in vivo pharmacodynamics of three novel antileishmanial lead series","volume":"8","author":"Van den Kerkhof","year":"2018","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"issue":"1","key":"10.1016\/j.mimet.2020.105935_bb0145","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JCM.01416-07","article-title":"Comparison between quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for quantification of Leishmania parasites","volume":"46","author":"van der Meide","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"8","key":"10.1016\/j.mimet.2020.105935_bb0150","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1586\/14787210.2014.916614","article-title":"Treatment failure in leishmaniasis: drug-resistance or another (epi-) phenotype?","volume":"12","author":"Vanaerschot","year":"2014","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"10.1016\/j.mimet.2020.105935_bb0155","series-title":"Control of the leishmaniasis: report of a meeting of the WHO Expert Committe on the Control Leishmaniases","author":"WHO","year":"2010"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701220301597?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701220301597?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:29:09Z","timestamp":1777501749000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701220301597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":31,"alternative-id":["S0167701220301597"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2020.105935","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative evaluation of nucleic acid stabilizing reagents for RNA- and DNA-based Leishmania detection in blood as proxy for visceral burdens","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2020.105935","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"105935"}}