{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:25:39Z","timestamp":1792787139513,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Federal Ministry of Food, Agriculture and Consumer Protection","award":["819-589"],"award-info":[{"award-number":["819-589"]}]},{"name":"University of Giessen","id":[{"id":"https:\/\/ror.org\/033eqas34","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Justus-Liebig-Universit\u00e4t Gie\u00dfen","id":[{"id":"https:\/\/ror.org\/033eqas34","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.mimet.2020.105968","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T11:24:39Z","timestamp":1590751479000},"page":"105968","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Assessing efficacy of N-Acetyl-l-Cysteine\u2013Sodium Hydroxide on bacterial viability and enhanced recovery of Mycobacterium avium subsp. paratuberculosis from bovine colostrum"],"prefix":"10.1016","volume":"175","author":[{"given":"Abdulwahed Ahmed","family":"Hassan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izhar U.H.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Ganz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Wehrend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Failing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Eisenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Abdulmawjood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"B\u00fclte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2020.105968_bb0005","series-title":"Conference Proceedings. Jelgava May 2\u20133, 2019","first-page":"45","article-title":"Evaluation of microbiological quality of colostrum. Foodbalt 2019 13th Baltic Conference on Food Science and Technology \u201cFood. Nutrition. Well-Being.\u201d","author":"Baltrukova","year":"2019"},{"key":"10.1016\/j.mimet.2020.105968_bb0010","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1128\/JCM.00333-13","article-title":"Optimization of hexadecylpyridinium chloride decontamination for the culture of Mycobacterium avium subsp. paratuberculosis from milk","volume":"51","author":"Bradner","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0015","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1128\/JCM.00508-13","article-title":"Chemical decontamination with N-acetyl-L-cysteine\u2013sodium hydroxide improves recovery of viable Mycobacterium avium subsp. paratuberculosis organisms from cultured milk","volume":"51","author":"Bradner","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0020","doi-asserted-by":"crossref","first-page":"3694","DOI":"10.3168\/jds.2013-7730","article-title":"Short communication: application of an N-acetyl-L-cysteine-NaOH decontamination method for the recovery of viable Mycobacterium avium subspecies paratuberculosis from milk of naturally infected cows","volume":"97","author":"Bradner","year":"2014","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0025","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.mimet.2005.01.010","article-title":"Comparison of NaOH-N-acetyl cysteine and sulfuric acid decontamination methods for recovery of mycobacteria from clinical specimens","volume":"62","author":"Buijtels","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2020.105968_bb0030","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/00480169.1995.35871","article-title":"Determination of the optimum concentration of decontaminants for the primary isolation of Mycobacterium bovis","volume":"43","author":"Corner","year":"1995","journal-title":"N-Z. Vet. J."},{"key":"10.1016\/j.mimet.2020.105968_bb0035","series-title":"BMDP Statistical Software Manual","first-page":"1993","volume":"Vol. 1&2","author":"Dixon","year":"1993"},{"key":"10.1016\/j.mimet.2020.105968_bb0040","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1017\/S0950268815000977","article-title":"Within-herd prevalence thresholds for the detection of Mycobacterium avium subspecies paratuberculosis-positive dairy herds using boot swabs and liquid manure samples","volume":"144","author":"Donat","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.mimet.2020.105968_bb0045","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1757-4749-2-11","article-title":"Isolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP) from cattle in Ireland using both traditional culture and molecular based methods","volume":"27","author":"Douarre","year":"2010","journal-title":"Gut Pathol."},{"key":"10.1016\/j.mimet.2020.105968_bb0050","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1046\/j.1472-765x.2001.00979.x","article-title":"Comparative evaluation of four decontamination protocols for the isolation of Mycobacterium avium subsp. paratuberculosis from milk","volume":"33","author":"Dundee","year":"2001","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0055","first-page":"523","article-title":"Bacterial contamination of colostrum fed to newborn calves in Qu\u00e9bec dairy herds","volume":"43","author":"Fecteau","year":"2002","journal-title":"Can. Vet. J."},{"key":"10.1016\/j.mimet.2020.105968_bb0060","series-title":"Paratuberkulose. Amtliche Methodensammlung: Meldepflichtige Tierkrankheiten","author":"FLI","year":"2020"},{"key":"10.1016\/j.mimet.2020.105968_bb0065","first-page":"81","article-title":"Development of improved method for isolation of Mycobacterium avium subsp. paratuberculosis from bulk tank milk: effect of age of milk, centrifugation, and decontamination","volume":"69","author":"Gao","year":"2005","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.mimet.2020.105968_bb0070","series-title":"February 26\u201327","first-page":"44","article-title":"Microbial hazards associated with feeding colostrum. Proceedings of the 24th annual southwest nutrition and management conference","volume":"2009","author":"Godden","year":"2009"},{"key":"10.1016\/j.mimet.2020.105968_bb0080","doi-asserted-by":"crossref","first-page":"2428","DOI":"10.1128\/AEM.68.5.2428-2435.2002","article-title":"Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows\u2019 milk from approved dairy processing establishments in the United Kingdom","volume":"68","author":"Grant","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0075","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1128\/AEM.68.2.602-607.2002","article-title":"Effect of commercial-scale high-temperature, short-time pasteurization on the viability of Mycobacterium paratuberculosis in naturally infected cows\u2019 milk","volume":"68","author":"Grant","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0085","article-title":"Evaluation of different diagnostic methods for the detection of Mycobacterium avium subsp. paratuberculosis in boot swabs and liquid manure samples","volume":"18","author":"Hahn","year":"2017","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.mimet.2020.105968_bb0090","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/s13104-020-05019-z","article-title":"Quantitative assessment and impact of thermal treatment on quality of Holstein dairy cattle colostrum immunoglobulin and viscosity","volume":"13","author":"Hassan","year":"2020","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.mimet.2020.105968_bb0095","series-title":"Composition and hygiene of colostrum on modern Pennsylvania dairy farms. Cooperative Extension, DAS 11\u2013171","author":"Heinrichs","year":"2011"},{"key":"10.1016\/j.mimet.2020.105968_bb0100","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s12223-016-0488-1","article-title":"An intra-laboratory cultural and real-time PCR method comparison and evaluation for the detection of subclinical paratuberculosis in dairy herds","volume":"62","author":"Heuvelink","year":"2017","journal-title":"Folia Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0105","doi-asserted-by":"crossref","first-page":"878","DOI":"10.4315\/0362-028X-69.4.878","article-title":"Growth of Mycobacterium avium subsp. paratuberculosis in the presence of hexadecylpyridinium chloride, natamycin, and vancomycin","volume":"69","author":"Johansen","year":"2006","journal-title":"J. Food Prot."},{"key":"10.1016\/j.mimet.2020.105968_bb0110","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.prevetmed.2017.05.013","article-title":"Testing of milk replacers for Mycobacterium avium subsp. paratuberculosis by PCR and bacterial culture as a possible source for Johne\u2019s disease (paratuberculosis) in calves","volume":"144","author":"Khol","year":"2017","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.mimet.2020.105968_bb0115","first-page":"775","article-title":"Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria","volume":"87","author":"Kubica","year":"1963","journal-title":"Am. Rev. Respir. Dis."},{"key":"10.1016\/j.mimet.2020.105968_bb0120","first-page":"284","article-title":"Comments on the use of the new mucolytic agent, N-acetyl-L-cysteine, as a sputum digestant for the isolation of mycobacteria","volume":"89","author":"Kubica","year":"1964","journal-title":"Am. Rev. Respir. Dis."},{"key":"10.1016\/j.mimet.2020.105968_bb0125","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1136\/vr.103641","article-title":"Microbiological contamination of colostrum on Irish dairy farms","volume":"178","author":"McAloon","year":"2016","journal-title":"Vet. Rec."},{"key":"10.1016\/j.mimet.2020.105968_bb0130","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.cvfa.2004.06.005","article-title":"Managing the production, storage and delivery of colostrum","volume":"20","author":"McGuirk","year":"2004","journal-title":"Vet. Clin. North. Am. Food Anim. Pract."},{"key":"10.1016\/j.mimet.2020.105968_bb0135","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.3168\/jds.2011-5174","article-title":"Nationwide evaluation of quality and composition of colostrum on dairy farms in the United States","volume":"95","author":"Morrill","year":"2012","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0140","doi-asserted-by":"crossref","first-page":"4610","DOI":"10.3168\/jds.2008-1272","article-title":"Colostrum and milk as risk factors for infection with Mycobacterium avium subspecies paratuberculosis in dairy cattle","volume":"91","author":"Nielsen","year":"2008","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0145","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1128\/JCM.36.2.402-408.1998","article-title":"Effects of decontamination methods and culture conditions on viability of Mycobacterium ulcerans in the BACTEC system","volume":"36","author":"Palomino","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0150","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1128\/JCM.43.1.442-444.2005","article-title":"Cetylpyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage","volume":"43","author":"Pardini","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0155","doi-asserted-by":"crossref","first-page":"343","DOI":"10.4142\/jvs.2017.18.S1.343","article-title":"Potential biomarkers as an indicator of vertical transmission of Johne\u2019s disease in a Korean native cattle farm","volume":"31","author":"Park","year":"2017","journal-title":"J. Vet. Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0160","doi-asserted-by":"crossref","first-page":"8981","DOI":"10.3168\/jds.2016-11200","article-title":"Survey of bovine colostrum quality and hygiene on northern Victorian dairy farms","volume":"99","author":"Phipps","year":"2016","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0165","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1111\/1574-6976.12030","article-title":"The complex microbiota of raw milk","volume":"37","author":"Quigley","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.mimet.2020.105968_bb0170","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0202034","article-title":"Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces","volume":"13","author":"Sattar","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2020.105968_bb0175","doi-asserted-by":"crossref","first-page":"283","DOI":"10.17221\/1859-VETMED","article-title":"Detection methods for Mycobacterium avium subsp. paratuberculosis in milk and milk products: a review","volume":"53","author":"Slana","year":"2008","journal-title":"Vet. Med."},{"key":"10.1016\/j.mimet.2020.105968_bb0180","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1128\/jcm.1.5.411-413.1975","article-title":"Use of cetylpyridinium chloride and sodium chloride for the decontamination of sputum specimens that are transported to the laboratory for the isolation of Mycobacterium tuberculosis","volume":"1","author":"Smithwick","year":"1975","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0185","doi-asserted-by":"crossref","first-page":"3600","DOI":"10.3168\/jds.2008-1107","article-title":"Pasteurization of colostrum reduces the incidence of paratuberculosis","volume":"91","author":"Stabel","year":"2008","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0190","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.4315\/0362-028X-67.12.2719","article-title":"Efficacy of pasteurization conditions for the inactivation of Mycobacterium avium subsp. paratuberculosis in milk","volume":"67","author":"Stabel","year":"2004","journal-title":"J. Food Prot."},{"key":"10.1016\/j.mimet.2020.105968_bb0195","doi-asserted-by":"crossref","first-page":"6296","DOI":"10.3168\/jds.2014-8204","article-title":"Clinical disease and stage of lactation influence shedding of Mycobacterium avium subspecies paratuberculosis into milk and colostrum of naturally infected dairy cows","volume":"97","author":"Stabel","year":"2014","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0200","doi-asserted-by":"crossref","first-page":"2571","DOI":"10.3168\/jds.S0022-0302(05)72933-7","article-title":"Preventing bacterial contamination and proliferation during the harvest, storage, and feeding of fresh bovine colostrum","volume":"88","author":"Stewart","year":"2005","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0205","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.2460\/ajvr.1995.56.10.1322","article-title":"Isolation of Mycobacterium paratuberculosis from colostrum and milk of subclinically infected cows","volume":"56","author":"Streeter","year":"1995","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.mimet.2020.105968_bb0210","doi-asserted-by":"crossref","first-page":"3857","DOI":"10.3168\/jds.2007-0152","article-title":"Passive transfer of immunoglobulin G and preweaning health in Holstein calves fed a commercial colostrum replacer","volume":"90","author":"Swan","year":"2007","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mimet.2020.105968_bb0215","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1128\/jcm.30.1.166-171.1992","article-title":"Mycobacterium paratuberculosis cultured from milk and supramammary lymph nodes of infected asymptomatic cows","volume":"30","author":"Sweeney","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0220","series-title":"Entwicklung und Validierung eines fluoreszenzmikroskopischen Schnellnachweisverfahrens f\u00fcr lebensf\u00e4hige Mycobacterium avium ssp. paratuberculosis (MAP)\u2013Zellen in Milch und Sauglingsanfangsnahrung.","author":"Weirich","year":"2012"},{"key":"10.1016\/j.mimet.2020.105968_bb0225","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1128\/JCM.37.4.1077-1083.1999","article-title":"Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep","volume":"37","author":"Whittington","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2020.105968_bb0230","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.1469-0691.1998.tb00372.x","article-title":"Comparison of the effect of two decontamination procedures on the detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test","volume":"4","author":"Zabel","year":"1998","journal-title":"Clin. Microbiol. Infect."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701220304139?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701220304139?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T23:59:55Z","timestamp":1759103995000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701220304139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":46,"alternative-id":["S0167701220304139"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2020.105968","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assessing efficacy of N-Acetyl-l-Cysteine\u2013Sodium Hydroxide on bacterial viability and enhanced recovery of Mycobacterium avium subsp. paratuberculosis from bovine colostrum","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2020.105968","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2020 Published by Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"105968"}}