{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T18:19:53Z","timestamp":1780683593971,"version":"3.54.1"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2024,7]]},"DOI":"10.1016\/j.mimet.2024.106941","type":"journal-article","created":{"date-parts":[[2024,5,5]],"date-time":"2024-05-05T17:35:19Z","timestamp":1714930519000},"page":"106941","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Development and validation of a multi-target TaqMan qPCR method for detection of Borrelia burgdorferi sensu lato"],"prefix":"10.1016","volume":"222","author":[{"given":"S\u00e9bastien","family":"Mass\u00e9glia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magalie","family":"Ren\u00e9-Martellet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Rates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Hizo-Teufel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Margos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Bailly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2024.106941_bb0005","series-title":"Animal Health Analysis Methods - PCR - Part 2: Requirements and Recommendations for the Development and the Validation of Veterinary PCR - NF U47-600-2 AFNOR Editions","first-page":"51","author":"AFNOR","year":"2015"},{"key":"10.1016\/j.mimet.2024.106941_bb0010","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1186\/s12864-016-3016-4","article-title":"Recurrent evolution of host and vector association in bacteria of the Borrelia burgdorferi sensu lato species complex","volume":"17","author":"Becker","year":"2016","journal-title":"BMC Genomics"},{"key":"10.1016\/j.mimet.2024.106941_bb0015","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s10493-016-0012-y","article-title":"Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans","volume":"69","author":"Briciu","year":"2016","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.mimet.2024.106941_bb0020","series-title":"The Ticks of the World (Acarida, Ixolida): Nomenclature, Described stages, Hosts, Distribution, \u00c9ditions de l'Orstom","author":"Camicas","year":"1998"},{"key":"10.1016\/j.mimet.2024.106941_bb0025","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1128\/JCM.42.7.3164-3168.2004","article-title":"Multiplex real-time PCR for detection of Anaplasma phagocytophilum and Borrelia burgdorferi","volume":"42","author":"Courtney","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0011147","article-title":"progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement","volume":"5","author":"Darling","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2024.106941_bb0035","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.mimet.2014.09.001","article-title":"Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR","volume":"107","author":"de Leeuw","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2024.106941_bb0040","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mimet.2024.106941_bb0045","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1186\/s13071-016-1803-z","article-title":"Nested coevolutionary networks shape the ecological relationships of ticks, hosts, and the Lyme disease bacteria of the Borrelia burgdorferi (s.l.) complex","volume":"9","author":"Estrada-Pena","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.mimet.2024.106941_bb0050","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.mimet.2010.04.009","article-title":"Evaluation of real-time PCR targeting hbb gene for Borrelia species identification","volume":"82","author":"Ferdin","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2024.106941_bb0055","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/37551","article-title":"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi","volume":"390","author":"Fraser","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.mimet.2024.106941_bb0060","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1051\/parasite\/2008153244","article-title":"Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis: life in the wilds","volume":"15","author":"Gern","year":"2008","journal-title":"Parasite"},{"key":"10.1016\/j.mimet.2024.106941_bb0065","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/j.1752-4571.2010.00127.x","article-title":"Cryptic vector divergence masks vector-specific patterns of infection: an example from the marine cycle of Lyme borreliosis","volume":"3","author":"G\u00f3mez-D\u00edaz","year":"2010","journal-title":"Evol. Appl."},{"key":"10.1016\/j.mimet.2024.106941_bb0070","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1111\/j.1469-0691.2006.01509.x","article-title":"Evaluation of an internally controlled real-time PCR targeting the ospA gene for detection of Borrelia burgdorferi sensu lato DNA in cerebrospinal fluid","volume":"12","author":"Gooskens","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2024.106941_bb0075","doi-asserted-by":"crossref","first-page":"7273","DOI":"10.1128\/AEM.02158-13","article-title":"Species co-occurrence patterns among Lyme borreliosis pathogens in the tick vector Ixodes ricinus","volume":"79","author":"Herrmann","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0080","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/1462-2920.12310","article-title":"Borrelia chilensis, a new member of the Borrelia burgdorferi sensu lato complex that extends the range of this genospecies in the southern hemisphere","volume":"16","author":"Ivanova","year":"2014","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-60327-353-4_1","article-title":"Real-time quantitative PCR, pathogen detection and MIQE","volume":"943","author":"Johnson","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.mimet.2024.106941_bb0090","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1017\/S0031182016001864","article-title":"Validation of droplet digital PCR for the detection and absolute quantification of Borrelia DNA in Ixodes scapularis ticks","volume":"144","author":"King","year":"2017","journal-title":"Parasitology"},{"key":"10.1016\/j.mimet.2024.106941_bb0095","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3389\/fmicb.2017.00108","article-title":"A basic guide to real time PCR in microbial diagnostics: definitions, parameters, and everything","volume":"8","author":"Kralik","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0100","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0185434","article-title":"Molecular detection of Borrelia burgdorferi sensu lato - an analytical comparison of real-time PCR protocols from five different Scandinavian laboratories","volume":"12","author":"Lager","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2024.106941_bb0105","doi-asserted-by":"crossref","first-page":"3276","DOI":"10.1093\/bioinformatics\/btu531","article-title":"AliView: a fast and lightweight alignment viewer and editor for large datasets","volume":"30","author":"Larsson","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.mimet.2024.106941_bb0110","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1186\/s13071-015-1043-7","article-title":"Reliability of molecular host-identification methods for ticks: an experimental in vitro study with Ixodes ricinus","volume":"8","author":"Leger","year":"2015","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.mimet.2024.106941_bb0115","doi-asserted-by":"crossref","first-page":"153","DOI":"10.14411\/fp.2006.020","article-title":"Effect of salivary gland extract from Ixodes ricinus ticks on the proliferation of Borrelia burgdorferi sensu stricto in vivo","volume":"53","author":"Machackova","year":"2006","journal-title":"Folia Parasitol."},{"key":"10.1016\/j.mimet.2024.106941_bb0120","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s10096-016-2797-3","article-title":"Technical and clinical validation of three commercial real-time PCR kits for the diagnosis of neuroborreliosis in cerebrospinal fluid on three different real-time PCR platforms","volume":"36","author":"Maes","year":"2017","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2024.106941_bb0125","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s004360050352","article-title":"18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae)","volume":"84","author":"Mangold","year":"1998","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.mimet.2024.106941_bb0130","doi-asserted-by":"crossref","first-page":"2830","DOI":"10.1128\/jcm.30.11.2830-2834.1992","article-title":"Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis","volume":"30","author":"Marconi","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0135","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/j.meegid.2011.07.022","article-title":"Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato","volume":"11","author":"Margos","year":"2011","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.mimet.2024.106941_bb0140","doi-asserted-by":"crossref","DOI":"10.3389\/fevo.2019.00401","article-title":"Borrelia bavariensis: vector switch, niche invasion, and geographical spread of a tick-borne bacterial parasite","volume":"7","author":"Margos","year":"2019","journal-title":"Front. Ecol. Evol."},{"key":"10.1016\/j.mimet.2024.106941_bb0145","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0055377","article-title":"Introduced Siberian chipmunks (Tamias sibiricus barberi) contribute more to Lyme Borreliosis risk than native reservoir rodents","volume":"8","author":"Marsot","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2024.106941_bb0150","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1007\/s00248-020-01512-x","article-title":"\u201cCandidatus Borrelia ibitipoquensis,\u201d a Borrelia valaisiana-related Genospecies characterized from Ixodes paranaensis in Brazil","volume":"80","author":"Munoz-Leal","year":"2020","journal-title":"Microb. Ecol."},{"key":"10.1016\/j.mimet.2024.106941_bb0155","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ttbdis.2017.09.001","article-title":"Development and evaluation of a two-step multiplex TaqMan real-time PCR assay for detection\/quantification of different genospecies of Borrelia burgdorferi sensu lato","volume":"9","author":"Nunes","year":"2018","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.mimet.2024.106941_bb0160","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-37195-0_13","article-title":"Assembling genomes and mini-metagenomes from highly chimeric reads","volume":"158-170","author":"Nurk","year":"2013","journal-title":"Res. Comput. Mol. Biol."},{"key":"10.1016\/j.mimet.2024.106941_bb0165","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1089\/vbz.2006.6.103","article-title":"A reverse transcriptase-polymerase chain reaction assay of Borrelia burgdorferi 16S rRNA for highly sensitive quantification of pathogen load in a vector","volume":"6","author":"Ornstein","year":"2006","journal-title":"Vector Borne Zoonotic Dis."},{"key":"10.1016\/j.mimet.2024.106941_bb0170","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/j.1574-6968.2006.00249.x","article-title":"A single-run, real-time PCR for detection and identification of Borrelia burgdorferi sensu lato species, based on the hbb gene sequence","volume":"259","author":"Portnoi","year":"2006","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2024.106941_bb0175","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1099\/00207713-44-4-743","article-title":"Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons","volume":"44","author":"Postic","year":"1994","journal-title":"Int. J. Syst. Bacteriol."},{"key":"10.1016\/j.mimet.2024.106941_bb0180","first-page":"Doc03","article-title":"Guidelines for diagnosis and treatment in neurology - Lyme neuroborreliosis","volume":"18","author":"Rauer","year":"2020","journal-title":"Ger. Med. Sci."},{"key":"10.1016\/j.mimet.2024.106941_bb0185","doi-asserted-by":"crossref","first-page":"7203","DOI":"10.1128\/AEM.71.11.7203-7216.2005","article-title":"Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis","volume":"71","author":"Rauter","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0190","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1128\/JCM.37.7.2215-2222.1999","article-title":"Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks","volume":"37","author":"Schouls","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0195","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1093\/cid\/ciy614","article-title":"Direct diagnostic tests for Lyme disease","volume":"68","author":"Schutzer","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.mimet.2024.106941_bb0200","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1046\/j.1198-743x.2001.00282.x","article-title":"Routine diagnosis of Borrelia burgdorferi (sensu lato) infections using a real-time PCR assay","volume":"7","author":"Schwaiger","year":"2001","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.mimet.2024.106941_bb0205","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0140-6736(11)60103-7","article-title":"Lyme borreliosis","volume":"379","author":"Stanek","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.mimet.2024.106941_bb0210","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1007\/s00253-020-10375-8","article-title":"Novel targets and strategies to combat borreliosis","volume":"104","author":"Strnad","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2024.106941_bb0215","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/1756-3305-3-69","article-title":"Establishment of a minor groove binder-probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia spielmanii by ospA-specific conventional PCR","volume":"3","author":"Strube","year":"2010","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.mimet.2024.106941_bb0220","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1128\/JCM.02544-10","article-title":"Multilocus sequence typing implicates rodents as the main reservoir host of human-pathogenic Borrelia garinii in Japan","volume":"49","author":"Takano","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2024.106941_bb0225","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1177\/1040638720960829","article-title":"Suggested guidelines for validation of real-time PCR assays in veterinary diagnostic laboratories","volume":"32","author":"Toohey-Kurth","year":"2020","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.mimet.2024.106941_bb0230","doi-asserted-by":"crossref","first-page":"18159","DOI":"10.1073\/pnas.0405763102","article-title":"An ecological approach to preventing human infection: vaccinating wild mouse reservoirs intervenes in the Lyme disease cycle","volume":"101","author":"Tsao","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.mimet.2024.106941_bb0235","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s15010-015-0820-8","article-title":"A novel duplex real-time PCR permits simultaneous detection and differentiation of Borrelia miyamotoi and Borrelia burgdorferi sensu lato","volume":"44","author":"Venczel","year":"2016","journal-title":"Infection"},{"key":"10.1016\/j.mimet.2024.106941_bb0240","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.ttbdis.2016.02.008","article-title":"Mapping human risk of infection with Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis, in a periurban forest in France","volume":"7","author":"Vourc'h","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.mimet.2024.106941_bb0245","first-page":"555","article-title":"PAML: a program package for phylogenetic analysis by maximum likelihood","volume":"13","author":"Yang","year":"1997","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.mimet.2024.106941_bb0250","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinformatics."}],"updated-by":[{"DOI":"10.1016\/j.mimet.2025.107253","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000}}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701224000538?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701224000538?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T05:32:39Z","timestamp":1716269559000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701224000538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":50,"alternative-id":["S0167701224000538"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2024.106941","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.07.18.549442","asserted-by":"object"}]},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of a multi-target TaqMan qPCR method for detection of Borrelia burgdorferi sensu lato","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2024.106941","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"106941"}}