{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:21:37Z","timestamp":1762255297551,"version":"build-2065373602"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006247","name":"Anhui University of Science and Technology","doi-asserted-by":"publisher","award":["YZ2023H1B003"],"award-info":[{"award-number":["YZ2023H1B003"]}],"id":[{"id":"10.13039\/501100006247","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.mimet.2025.107121","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T21:00:56Z","timestamp":1743022856000},"page":"107121","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["The development of a subunit vaccine for Mycobacterium tuberculosis Rv0081 as a booster for BCG and the investigation of its immunogenicity"],"prefix":"10.1016","volume":"232-234","author":[{"given":"Yun","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiangsen","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaochun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinkuang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LingYun","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Runlin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2025.107121_bb0005","first-page":"30","article-title":"Recent advances in understanding the Th1\/Th2 effector choice[J]","volume":"10","author":"Butcher","year":"2021","journal-title":"Journa"},{"issue":"6","key":"10.1016\/j.mimet.2025.107121_bb0010","first-page":"1605","article-title":"Safety and immunogenicity of the ID93+ GLA-SE tuberculosis vaccine in BCG-vaccinated healthy adults: a randomized, double-blind, placebo-controlled phase 2 trial[J]","volume":"12","author":"Choi","year":"2023","journal-title":"Journa"},{"issue":"10","key":"10.1016\/j.mimet.2025.107121_bb0015","first-page":"1472","article-title":"Safety and immunogenicity of VPM1002versus BCG in south African newborn babies: a randomised, phase 2 non-inferiority double-blind controlled trial[J]","volume":"22","author":"Cotton","year":"2022","journal-title":"Journa"},{"issue":"4","key":"10.1016\/j.mimet.2025.107121_bb0020","first-page":"373","article-title":"Safety and immunogenicity of the adjunct therapeutic vaccine ID93+GLA-SE in adults who have completed treatment for tuberculosis: a randomised, double-blind, placebo-controlled, phase 2a trial[J]","volume":"9","author":"Day","year":"2021","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0025","article-title":"Tuberculosis vaccine: a journey from BCG to present[J]","volume":"252","author":"Fatima","year":"2020","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0030","first-page":"145","article-title":"Developments in vaccine adjuvants[J]","volume":"2412","author":"Firdaus","year":"2022","journal-title":"Journa"},{"issue":"1","key":"10.1016\/j.mimet.2025.107121_bb0035","first-page":"6774","article-title":"A phase I\/II randomized trial of H56:IC31 vaccination and adjunctive cyclooxygenase-2-inhibitor treatment in tuberculosis patients[J]","volume":"12","author":"Jenum","year":"2021","journal-title":"Journa"},{"issue":"1","key":"10.1016\/j.mimet.2025.107121_bb0040","first-page":"2721","article-title":"Insights into the molecular determinants involved in Mycobacterium tuberculosis persistence and their therapeutic implications[J]","volume":"12","author":"Joshi","year":"2021","journal-title":"Journa"},{"issue":"6","key":"10.1016\/j.mimet.2025.107121_bb0045","first-page":"784","article-title":"Biosensors for the detection of Mycobacterium tuberculosis: a comprehe sive overview[J]","volume":"48","author":"Joshi","year":"2022","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0050","article-title":"Applications of transcriptomics and proteomics for understanding dormancy and resuscitation in Mycobacterium tuberculosis[J]","volume":"12","author":"Kundu","year":"2021","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0055","article-title":"Enhanced immunogenicity of the tuberculosis subunit Rv0572c vaccine delivered in DMT liposome adjuvant as a BCG-booster[J]","volume":"134","author":"Mao","year":"2022","journal-title":"Journa"},{"issue":"4","key":"10.1016\/j.mimet.2025.107121_bb0060","article-title":"Intricate genetic programs controlling dormancy in Mycobacterium tuberculosis[J]","volume":"31","author":"Peterson","year":"2020","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0065","first-page":"180","article-title":"Functional signatures of human CD4 and CD8 T cell responses to Mycobacterium tuberculosis[J]","volume":"5","author":"Prezzemolo","year":"2014","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0070","article-title":"Late stage specific Rv0109 (PE_PGRS1) protein of Mycobacterium tuberculosis induces mitochondria mediated macrophage apoptosis[J]","volume":"176","author":"Priyanka","year":"2023","journal-title":"Journa"},{"issue":"4","key":"10.1016\/j.mimet.2025.107121_bb0075","article-title":"Mycobacterium tuberculosis protein PPE15 (Rv1039c) possesses eukaryote-like SH3 domain that interferes with NADPH oxidase assembly and reactive oxygen species production[J]","volume":"1871","author":"Priyanka","year":"2024","journal-title":"Journa"},{"issue":"3","key":"10.1016\/j.mimet.2025.107121_bb0080","article-title":"A multistage antigen complex Epera013 promotes efficient and comprehensive immune responses in BALB\/c mice[J]","volume":"11","author":"Qian","year":"2023","journal-title":"Journa"},{"issue":"12","key":"10.1016\/j.mimet.2025.107121_bb0085","first-page":"5284","article-title":"BCG vaccination strategies against tuberculosis: updates and perspectives[J]","volume":"17","author":"Qu","year":"2021","journal-title":"Journa"},{"issue":"12","key":"10.1016\/j.mimet.2025.107121_bb0090","article-title":"Safety and immunogenicity of H1\/IC31\u00ae, an adjuvanted TB subunit vaccine, in HIV-infected adults with CD4+ lymphocyte counts greater than 350 cells\/mm3: a phase II, multi-Centre, double-blind, randomized, placebo-controlled trial[J]","volume":"9","author":"Reither","year":"2014","journal-title":"Journa"},{"issue":"1","key":"10.1016\/j.mimet.2025.107121_bb0095","first-page":"715","article-title":"Adjuvant system AS01: from mode of action to effective vaccines[J]","volume":"23","author":"Roman","year":"2024","journal-title":"Journa"},{"issue":"3","key":"10.1016\/j.mimet.2025.107121_bb0100","first-page":"e28","article-title":"The status of tuberculosis vaccine development[J]","volume":"20","author":"Schrager","year":"2020","journal-title":"Journa"},{"issue":"2","key":"10.1016\/j.mimet.2025.107121_bb0105","first-page":"220","article-title":"Dose optimization of H56:IC31 vaccine for tuberculosis-endemic populations. a double-blind, placebo-controlled, dose-selection trial[J]","volume":"199","author":"Suliman","year":"2019","journal-title":"Journa"},{"issue":"1","key":"10.1016\/j.mimet.2025.107121_bb0110","article-title":"Enhancing TB vaccine efficacy: current Progress on vaccines, adjuvants and immunization strategies[J]","volume":"12","author":"Wang","year":"2023","journal-title":"Journa"},{"year":"2023","series-title":"Global Tuberculosis Report 2023","author":"World Health Organization","key":"10.1016\/j.mimet.2025.107121_bb0115"},{"author":"World Health Organization","key":"10.1016\/j.mimet.2025.107121_bb0120"},{"key":"10.1016\/j.mimet.2025.107121_bb0125","first-page":"226","article-title":"Immunogenicity and protective efficacy of a fusion protein tuberculosis vaccine combining five Esx family proteins[J]","volume":"7","author":"Xiang","year":"2017","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0130","first-page":"90 (2)","article-title":"COVID-19: avoiding a second tragedy in a tuberculosis burdened country[J]","author":"Yadav","year":"2020","journal-title":"Journa"},{"issue":"6","key":"10.1016\/j.mimet.2025.107121_bb0135","first-page":"691","article-title":"Progress on diagnosis and treatment of latent tuberculosis infection[J]","volume":"51","author":"Ying","year":"2022","journal-title":"Journa"},{"key":"10.1016\/j.mimet.2025.107121_bb0140","article-title":"Advances in protein subunit vaccines against tuberculosis[J]","volume":"14","author":"Zhang","year":"2023","journal-title":"Journa"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701225000375?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701225000375?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:08:33Z","timestamp":1762254513000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701225000375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":28,"alternative-id":["S0167701225000375"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107121","relation":{},"ISSN":["0167-7012"],"issn-type":[{"type":"print","value":"0167-7012"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The development of a subunit vaccine for Mycobacterium tuberculosis Rv0081 as a booster for BCG and the investigation of its immunogenicity","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107121","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107121"}}