{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:21:33Z","timestamp":1762255293129,"version":"build-2065373602"},"reference-count":18,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004681","name":"Higher Education Commission, Pakistan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.mimet.2025.107191","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T02:32:19Z","timestamp":1751941939000},"page":"107191","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["styMdtM gene deletion with modified \u03bb Red recombineering approach: Implications for multidrug resistance in Salmonella Typhi"],"prefix":"10.1016","volume":"236","author":[{"given":"Anam","family":"Tariq","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aqsa","family":"Shaheen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mazhar","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moazur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2025.107191_bb0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/6432580","article-title":"Extensively drug-resistant (XDR) typhoid: evolution, prevention, and its management","volume":"2020","author":"Akram","year":"2020","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.mimet.2025.107191_bb0010","first-page":"363","article-title":"Molecular analysis of drug resistance in clinical isolates of MDR Salmonella enterica Serovar Typhi in Faisalabad, Pakistan","volume":"41","author":"Bashir","year":"2009","journal-title":"Pak. J. Zool."},{"key":"10.1016\/j.mimet.2025.107191_bb0015","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/mmi.15209","article-title":"Defying the odds: determinants of the antimicrobial response of Salmonella Typhi and their interplay","volume":"121","author":"Chowdhury","year":"2024","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mimet.2025.107191_bb0020","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mimet.2025.107191_bb0025","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.gene.2006.04.018","article-title":"A set of recombineering plasmids for gram-negative bacteria","volume":"379","author":"Datta","year":"2006","journal-title":"Gene"},{"key":"10.1016\/j.mimet.2025.107191_bb0030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micpath.2020.104096","article-title":"Gene interaction network studies to decipher the multi-drug resistance mechanism in Salmonella enterica serovar Typhi CT18 reveal potential drug targets","volume":"142","author":"Debroy","year":"2020","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.mimet.2025.107191_bb0040","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/S0966-842X(01)02067-4","article-title":"The molecular mechanisms of severe typhoid fever","volume":"9","author":"Everest","year":"2001","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.mimet.2025.107191_bb0045","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1128\/AEM.02509-08","article-title":"Rapid oligonucleotide-based recombineering of the chromosome of Salmonella enterica","volume":"75","author":"Gerlach","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mimet.2025.107191_bb0050","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/j.biochi.2012.03.001","article-title":"Functional and biochemical characterisation of the Escherichia coli major facilitator superfamily multidrug transporter MdtM","volume":"94","author":"Holdsworth","year":"2012","journal-title":"Biochimie"},{"key":"10.1016\/j.mimet.2025.107191_bb0070","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.bbapap.2009.02.002","article-title":"Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella","volume":"1794","author":"Nishino","year":"2009","journal-title":"Biochim. Biophys. Acta Proteins Proteomics"},{"key":"10.1016\/j.mimet.2025.107191_bb0075","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/35101607","article-title":"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18","volume":"413","author":"Parkhill","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.mimet.2025.107191_bb0080","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1128\/MMBR.64.4.672-693.2000","article-title":"Molecular properties of bacterial multidrug transporters","volume":"64","author":"Putman","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.mimet.2025.107191_bb0100","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.jiac.2015.01.002","article-title":"Characterization of putative multidrug resistance transporters of the major facilitator-superfamily expressed in Salmonella Typhi","volume":"21","author":"Shaheen","year":"2015","journal-title":"J. Infect. Chemother."},{"key":"10.1016\/j.mimet.2025.107191_bb0105","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1002\/prot.26141","article-title":"Characterization of the multidrug efflux transporter styMdtM from Salmonella enterica serovar Typhi","volume":"89","author":"Shaheen","year":"2021","journal-title":"Proteins: Struct., Funct., Bioinf."},{"key":"10.1016\/j.mimet.2025.107191_bb0110","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/nprot.2008.227","article-title":"Recombineering: a homologous recombination-based method of genetic engineering","volume":"4","author":"Sharan","year":"2009","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mimet.2025.107191_bb0115","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mimet.2025.107191_bb0120","doi-asserted-by":"crossref","first-page":"5978","DOI":"10.1073\/pnas.100127597","article-title":"An efficient recombination system for chromosome engineering in Escherichia coli","volume":"97","author":"Yu","year":"2000","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mimet.2025.107191_bb0125","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s00253-011-3317-0","article-title":"A method to generate recombinant Salmonella Typhi Ty21a strains expressing multiple heterologous genes using an improved recombineering strategy","volume":"91","author":"Yu","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701225001071?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701225001071?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:08:27Z","timestamp":1762254507000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701225001071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":18,"alternative-id":["S0167701225001071"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107191","relation":{},"ISSN":["0167-7012"],"issn-type":[{"type":"print","value":"0167-7012"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"styMdtM gene deletion with modified \u03bb Red recombineering approach: Implications for multidrug resistance in Salmonella Typhi","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107191","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107191"}}