{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:13:58Z","timestamp":1793661238957,"version":"4.5.1"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.mimet.2025.107291","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:10:55Z","timestamp":1760145055000},"page":"107291","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Machine learning powered profiling: Rapid identification of Klebsiella Pneumoniae drug resistance from MALDI-TOF MS"],"prefix":"10.1016","volume":"238","author":[{"given":"Xiaobo","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuntao","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.mimet.2025.107291_bb0005","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1128\/CMR.00072-12","article-title":"Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology","volume":"26","author":"Clark","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"issue":"20","key":"10.1016\/j.mimet.2025.107291_bb0010","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1161\/CIRCULATIONAHA.115.001593","article-title":"Machine learning in medicine","volume":"132","author":"Deo","year":"2015","journal-title":"Circulation"},{"issue":"7","key":"10.1016\/j.mimet.2025.107291_bb0015","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00464-21","article-title":"Rapid and accurate detection of aminoglycoside-modifying enzymes and 16S rRNA methyltransferases by targeted liquid chromatography-tandem mass spectrometry","volume":"59","author":"Foudraine","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2025.107291_bb0020","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.mimet.2018.11.008","article-title":"Rapid detection of colistin-resistant Klebsiella pneumoniae using MALDI-TOF MS peak-based assay","volume":"155","author":"Giordano","year":"2018","journal-title":"J. Microbiol. Methods"},{"issue":"7","key":"10.1016\/j.mimet.2025.107291_bb0025","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/S1473-3099(17)30228-1","article-title":"Effect of appropriate combination therapy on mortality of patients with bloodstream infections due to carbapenemase-producing Enterobacteriaceae (INCREMENT): a retrospective cohort study","volume":"17","author":"Guti\u00e9rrez-Guti\u00e9rrez","year":"2017","journal-title":"Lancet Infect. Dis."},{"issue":"3","key":"10.1016\/j.mimet.2025.107291_bb0030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1007608","article-title":"Machine learning with random subspace ensembles identifies antimicrobial resistance determinants from pan-genomes of three pathogens","volume":"16","author":"Hyun","year":"2020","journal-title":"PLoS Comput. Biol."},{"issue":"12","key":"10.1016\/j.mimet.2025.107291_bb0035","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01814-19","article-title":"Matrix-assisted laser desorption ionization-time of flight mass spectrometry for antimicrobial susceptibility testing","volume":"59","author":"Idelevich","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2025.107291_bb0040","doi-asserted-by":"crossref","DOI":"10.2196\/58039","article-title":"Pioneering Klebsiella pneumoniae antibiotic resistance prediction with artificial intelligence-clinical decision support system-enhanced matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry: retrospective study","volume":"26","author":"Jian","year":"2024","journal-title":"J. Med. Internet Res."},{"issue":"4","key":"10.1016\/j.mimet.2025.107291_bb0045","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1074\/mcp.RA118.000739","article-title":"Decreased antibiotic susceptibility driven by global remodeling of the Klebsiella pneumoniae proteome","volume":"18","author":"Keasey","year":"2019","journal-title":"Mol. Cell. Proteomics"},{"issue":"3","key":"10.1016\/j.mimet.2025.107291_bb0050","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.201910264","article-title":"Predicting antimicrobial resistance in Pseudomonas aeruginosa with machine learning-enabled molecular diagnostics","volume":"12","author":"Khaledi","year":"2020","journal-title":"EMBO Mol. Med."},{"issue":"21","key":"10.1016\/j.mimet.2025.107291_bb0055","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242115605","article-title":"Raman-based techniques in medical applications for diagnostic tasks: a review","volume":"24","author":"Khristoforova","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"12","key":"10.1016\/j.mimet.2025.107291_bb0060","first-page":"4442","article-title":"A rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for single-plasmid tracking in mice","volume":"52","author":"Lau","year":"2014","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.mimet.2025.107291_bb0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.jiph.2024.102541","article-title":"Artificial intelligence-clinical decision support system for enhanced infectious disease management: accelerating ceftazidime-avibactam resistance detection in Klebsiella pneumoniae","volume":"17","author":"Lin","year":"2024","journal-title":"J. Infect. Public Health"},{"issue":"1","key":"10.1016\/j.mimet.2025.107291_bb0070","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02502-21","article-title":"Application of mNGS in the etiological analysis of lower respiratory tract infections and the prediction of drug resistance","volume":"10","author":"Liu","year":"2022","journal-title":"Microbiol. Spectr."},{"issue":"1","key":"10.1016\/j.mimet.2025.107291_bb0075","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s40121-021-00408-8","article-title":"The impact of Carbapenem resistance on mortality in patients with Klebsiella pneumoniae bloodstream infection: an individual patient data meta-analysis of 1952 patients","volume":"10","author":"Maraolo","year":"2021","journal-title":"Infect. Dis. Ther."},{"issue":"4","key":"10.1016\/j.mimet.2025.107291_bb0080","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1128\/CMR.15.4.647-679.2002","article-title":"Interactions among strategies associated with bacterial infection: pathogenicity, epidemicity, and antibiotic resistance","volume":"15","author":"Mart\u00ednez","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"10.1016\/j.mimet.2025.107291_bb0085","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/MMBR.00078-15","article-title":"Klebsiella pneumoniae: going on the offense with a strong defense","volume":"80","author":"Paczosa","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.mimet.2025.107291_bb0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijmmb.2023.100432","article-title":"Introducing the new face of CLSI M100 in 2023: an explanatory review","volume":"46","author":"Rai","year":"2023","journal-title":"Indian J. Med. Microbiol."},{"issue":"4","key":"10.1016\/j.mimet.2025.107291_bb0095","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2020.2899","article-title":"Prevalence of antibiotic-resistant pathogens in culture-proven Sepsis and outcomes associated with inadequate and broad-spectrum empiric antibiotic use","volume":"3","author":"Rhee","year":"2020","journal-title":"JAMA Netw. Open"},{"issue":"Suppl. 1","key":"10.1016\/j.mimet.2025.107291_bb0100","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1086\/655995","article-title":"Progress and challenges in implementing the research on ESKAPE pathogens","volume":"31","author":"Rice","year":"2010","journal-title":"Infect. Control Hosp. Epidemiol."},{"issue":"7491","key":"10.1016\/j.mimet.2025.107291_bb0105","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nature13118","article-title":"The present and future role of microfluidics in biomedical research","volume":"507","author":"Sackmann","year":"2014","journal-title":"Nature"},{"issue":"3","key":"10.1016\/j.mimet.2025.107291_bb0110","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1002\/mas.21359","article-title":"MALDI TOF MS profiling of bacteria at the strain level: a review","volume":"32","author":"Sandrin","year":"2013","journal-title":"Mass Spectrom. Rev."},{"issue":"4","key":"10.1016\/j.mimet.2025.107291_bb0115","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1086\/600885","article-title":"Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry","volume":"49","author":"Seng","year":"2009","journal-title":"Clin. Infect. Dis."},{"issue":"10","key":"10.1016\/j.mimet.2025.107291_bb0120","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.3390\/microorganisms11102537","article-title":"Epidemiology and resistance phenotypes of Carbapenem-resistant Klebsiella pneumoniae in Corfu general hospital (2019-2022): a comprehensive time series analysis of resistance gene dynamics","volume":"11","author":"Sorovos","year":"2023","journal-title":"Microorganisms"},{"issue":"1","key":"10.1016\/j.mimet.2025.107291_bb0125","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41579-018-0098-9","article-title":"Developmental roadmap for antimicrobial susceptibility testing systems","volume":"17","author":"Van Belkum","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2025.107291_bb0130","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1038\/s41591-021-01619-9","article-title":"Direct antimicrobial resistance prediction from clinical MALDI-TOF mass spectra using machine learning","volume":"28","author":"Weis","year":"2022","journal-title":"Nat. Med."},{"issue":"1","key":"10.1016\/j.mimet.2025.107291_bb0135","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/JCM.01643-15","article-title":"Clinical performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry method for detection of certain Bla<sub>KPC<\/sub>\u2212containing plasmids","volume":"54","author":"Youn","year":"2016","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.mimet.2025.107291_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijantimicag.2023.106799","article-title":"Direct prediction of carbapenem-resistant, carbapenemase-producing, and colistin-resistant Klebsiella pneumoniae isolates from routine MALDI-TOF mass spectra using machine learning and outcome evaluation","volume":"61","author":"Yu","year":"2023","journal-title":"Int. J. Antimicrob. Agents"},{"issue":"6","key":"10.1016\/j.mimet.2025.107291_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijantimicag.2023.106994","article-title":"Prediction of methicillin-resistant Staphylococcus aureus and carbapenem-resistant Klebsiella pneumoniae from flagged blood cultures by combining rapid Sepsityper MALDI-TOF mass spectrometry with machine learning","volume":"62","author":"Yu","year":"2023","journal-title":"Int. J. Antimicrob. Agents"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701225002076?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701225002076?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T22:23:06Z","timestamp":1763504586000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701225002076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":29,"alternative-id":["S0167701225002076"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107291","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Machine learning powered profiling: Rapid identification of Klebsiella Pneumoniae drug resistance from MALDI-TOF MS","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107291","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107291"}}