{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T11:54:59Z","timestamp":1768996499522,"version":"3.49.0"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/L015129\/1"],"award-info":[{"award-number":["BB\/L015129\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011636","name":"March of Dimes Prematurity Research Center Ohio Collaborative","doi-asserted-by":"publisher","award":["22-FY18-82"],"award-info":[{"award-number":["22-FY18-82"]}],"id":[{"id":"10.13039\/100011636","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["104931\/Z\/14\/Z"],"award-info":[{"award-number":["104931\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020621","name":"Seoul St. Mary's Hospital, Catholic University of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000762","name":"Imperial College Healthcare NHS Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013342","name":"NIHR Imperial Biomedical Research Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013342","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000761","name":"Imperial College London","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000761","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.mimet.2025.107358","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T16:43:36Z","timestamp":1765471416000},"page":"107358","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Vaginal microbe media (VMM): a novel medium to culture Lactobacillus iners and other fastidious vaginal bacteria"],"prefix":"10.1016","volume":"240","author":[{"given":"Lauren A.","family":"Roberts","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C.A.","family":"Gaboriau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"MacIntyre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian R.","family":"Marchesi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.mimet.2025.107358_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2023.112474","article-title":"Vaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling","volume":"42","author":"Berard","year":"2023","journal-title":"Cell Rep."},{"issue":"3","key":"10.1016\/j.mimet.2025.107358_bb0010","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1038\/s41564-022-01070-7","article-title":"Cysteine dependence of Lactobacillus iners is a potential therapeutic target for vaginal microbiota modulation","volume":"7","author":"Bloom","year":"2022","journal-title":"Nat. Microbiol."},{"issue":"11","key":"10.1016\/j.mimet.2025.107358_bb0015","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1016\/j.ccell.2023.09.012","article-title":"Tumor-resident Lactobacillus iners confer chemoradiation resistance through lactate-induced metabolic rewiring","volume":"41","author":"Colbert","year":"2023","journal-title":"Cancer Cell"},{"issue":"7","key":"10.1016\/j.mimet.2025.107358_bb0020","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/nmeth.2075","article-title":"Icy: an open bioimage informatics platform for extended reproducible research","volume":"9","author":"De Chaumont","year":"2012","journal-title":"Nat. Methods"},{"issue":"3","key":"10.1016\/j.mimet.2025.107358_bb0025","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/s41564-022-01083-2","article-title":"Towards a deeper understanding of the vaginal microbiota","volume":"7","author":"France","year":"2022","journal-title":"Nat. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2025.107358_bb0030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10616-010-9263-3","article-title":"Albumin and mammalian cell culture: implications for biotechnology applications","volume":"62","author":"Francis","year":"2010","journal-title":"Cytotechnology"},{"issue":"5","key":"10.1016\/j.mimet.2025.107358_bb0035","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1128\/jcm.30.5.1323-1326.1992","article-title":"Defined medium simulating genital tract secretions for growth of vaginal microflora","volume":"30","author":"Geshnizgani","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mimet.2025.107358_bb0040","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"10.1016\/j.mimet.2025.107358_bb0045","author":"Hertzberger"},{"issue":"4","key":"10.1016\/j.mimet.2025.107358_bb0050","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s11908-023-00798-5","article-title":"Lactobacillus iners and genital health: molecular clues to an enigmatic vaginal species","volume":"25","author":"Holm","year":"2023","journal-title":"Curr. Infect. Dis. Rep."},{"issue":"3","key":"10.1016\/j.mimet.2025.107358_bb0055","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/intbio\/zyz006","article-title":"Microdroplet co-cultivation and interaction characterization of human vaginal bacteria","volume":"11","author":"Jackman","year":"2019","journal-title":"Integr. Biol."},{"issue":"9","key":"10.1016\/j.mimet.2025.107358_bb0060","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1038\/s41564-023-01447-2","article-title":"Bacterial amylases enable glycogen degradation by the vaginal microbiome","volume":"8","author":"Jenkins","year":"2023","journal-title":"Nat. Microbiol."},{"issue":"11","key":"10.1016\/j.mimet.2025.107358_bb0065","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1016\/j.tcb.2022.06.008","article-title":"Nutrient availability as an arbiter of cell size","volume":"32","author":"Kellogg","year":"2022","journal-title":"Trends Cell Biol."},{"issue":"11","key":"10.1016\/j.mimet.2025.107358_bb0070","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1038\/s41564-023-01500-0","article-title":"A citizen-science-enabled catalogue of the vaginal microbiome and associated factors","volume":"8","author":"Lebeer","year":"2023","journal-title":"Nat. Microbiol."},{"issue":"5","key":"10.1016\/j.mimet.2025.107358_bb0075","doi-asserted-by":"crossref","first-page":"775","DOI":"10.5851\/kosfa.2022.e46","article-title":"Review of the current research on fetal bovine serum and the development of cultured meat","volume":"42","author":"Lee","year":"2022","journal-title":"Food Sci. Anim. Resour."},{"key":"10.1016\/j.mimet.2025.107358_bb0080","article-title":"Exploring vaginal microbiome: from traditional methods to metagenomic next-generation sequencing\u2014a systematic review","volume":"16","author":"Liu","year":"2025","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2025.107358_bb0085","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1146\/annurev-micro-092611-150157","article-title":"Vaginal microbiome: rethinking health and disease","volume":"66","author":"Ma","year":"2012","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.mimet.2025.107358_bb0090","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.957158","article-title":"Biomarker enrichment medium: a defined medium for metabolomic analysis of microbial pathogens","volume":"13","author":"Mapar","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2025.107358_bb0095","article-title":"Bovine serum albumin promotes reactivation of viable but non-culturable mycobacterium tuberculosis via the activation of protein kinase-dependent cell division processes (preprint)","author":"Morishige","year":"2021","journal-title":"Cold Spring Harbor Laboratory."},{"issue":"1","key":"10.1016\/j.mimet.2025.107358_bb0100","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/s41522-025-00783-x","article-title":"ABO blood group antigens influence host\u2013microbe interactions and risk of early spontaneous preterm birth","volume":"11","author":"Mountain","year":"2025","journal-title":"Npj Biofilms and Microbiomes"},{"issue":"supplement_1","key":"10.1016\/j.mimet.2025.107358_bb0105","doi-asserted-by":"crossref","first-page":"4680","DOI":"10.1073\/pnas.1002611107","article-title":"Vaginal microbiome of reproductive-age women","volume":"108","author":"Ravel","year":"2011","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mimet.2025.107358_bb0110","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/645983","article-title":"Metabolic regulation of a bacterial cell system with emphasis on Escherichia coli metabolism","volume":"2013","author":"Shimizu","year":"2013","journal-title":"ISRN Biochemistry"},{"issue":"6","key":"10.1016\/j.mimet.2025.107358_bb0115","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1038\/nprot.2013.066","article-title":"Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization","volume":"8","author":"Skinner","year":"2013","journal-title":"Nat. Protoc."},{"issue":"1","key":"10.1016\/j.mimet.2025.107358_bb0120","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms14123","article-title":"Optimality and sub-optimality in a bacterial growth law","volume":"8","author":"Towbin","year":"2017","journal-title":"Nat. Commun."},{"issue":"16","key":"10.1016\/j.mimet.2025.107358_bb0125","doi-asserted-by":"crossref","first-page":"3418","DOI":"10.1016\/j.bpj.2021.06.022","article-title":"Spatiotemporal dynamics of growth and death within spherical bacterial colonies","volume":"120","author":"Welker","year":"2021","journal-title":"Biophys. J."},{"issue":"4","key":"10.1016\/j.mimet.2025.107358_bb0130","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/cmi.12537","article-title":"Vaginal epithelial cells regulate membrane adhesiveness to co-ordinate bacterial adhesion: epithelial cells regulate membrane adhesiveness","volume":"18","author":"Younes","year":"2016","journal-title":"Cell. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2025.107358_bb0135","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/0011-2240(91)90003-7","article-title":"Factors influencing survival of mammalian cells exposed to hypothermia: V. Effects of hepes, free radicals, and H2O2 under light and dark conditions","volume":"28","author":"Zieger","year":"1991","journal-title":"Cryobiology"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770122500274X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770122500274X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T21:42:52Z","timestamp":1768945372000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016770122500274X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":27,"alternative-id":["S016770122500274X"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107358","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Vaginal microbe media (VMM): a novel medium to culture Lactobacillus iners and other fastidious vaginal bacteria","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2025.107358","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107358"}}