{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:06:44Z","timestamp":1784552804255,"version":"3.55.0"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012253","name":"Guangxi University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012253","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["AB24010161"],"award-info":[{"award-number":["AB24010161"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["AB241484045"],"award-info":[{"award-number":["AB241484045"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.mimet.2026.107494","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T17:02:09Z","timestamp":1775754129000},"page":"107494","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Development and validation of a VirB12-based indirect ELISA for differentiating Brucella abortus infection from A19-\u0394VirB12 vaccination"],"prefix":"10.1016","volume":"245","author":[{"given":"Ting","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanglin","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xindong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongwei","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuijin","family":"Teng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinping","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangqiang","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuilan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhong","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijian","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzhang","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2026.107494_bb0005","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.3390\/vaccines11071273","article-title":"Characterization of Brucella abortus Mutant A19mut2, a Potential DIVA Vaccine Candidate with a Modification on Lipopolysaccharide","volume":"11","author":"Abdelgawad","year":"2023","journal-title":"Vaccines-Basel"},{"key":"10.1016\/j.mimet.2026.107494_bb0010","doi-asserted-by":"crossref","DOI":"10.1186\/s12917-020-02346-y","article-title":"Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya","volume":"16","author":"Akoko","year":"2020","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.mimet.2026.107494_bb0015","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010816","article-title":"A health promotion perspective for the control and prevention of Brucellosis (Brucella melitensis); Israel as a case study","volume":"16","author":"Baron-Epel","year":"2022","journal-title":"Plos Neglect Trop. D"},{"key":"10.1016\/j.mimet.2026.107494_bb0020","first-page":"137","article-title":"Importance of brucellosis control programs of livestock on the improvement of one health","volume":"41","author":"Dadar","year":"2021","journal-title":"Vet. Quarter."},{"key":"10.1016\/j.mimet.2026.107494_bb0025","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2023.1255239","article-title":"Brucellae as resilient intracellular pathogens: epidemiology, host-pathogen interaction, recent genomics and proteomics approaches, and future perspectives","volume":"10","author":"Dawood","year":"2023","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.mimet.2026.107494_bb0030","doi-asserted-by":"crossref","first-page":"654","DOI":"10.3390\/vaccines11030654","article-title":"The development of diagnostic and vaccine strategies for early detection and control of human brucellosis, particularly in endemic areas","volume":"11","author":"Elbehiry","year":"2023","journal-title":"Vaccines-Basel"},{"key":"10.1016\/j.mimet.2026.107494_bb0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.prevetmed.2022.105771","article-title":"Bayesian evaluation of three serological tests for diagnosis of brucella infections in dromedary camels using latent class models","volume":"208","author":"Elsohaby","year":"2022","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.mimet.2026.107494_bb0040","doi-asserted-by":"crossref","DOI":"10.1107\/S0108767309099620","article-title":"Structure and assembly of a bacterial type IV secretion core complex","volume":"65","author":"Fronzes","year":"2009","journal-title":"Acta Crystallogr. Sect. A Found. Crystallogr."},{"key":"10.1016\/j.mimet.2026.107494_bb0045","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nrmicro2218","article-title":"The structural biology of type IV secretion systems","volume":"7","author":"Fronzes","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.mimet.2026.107494_bb0050","doi-asserted-by":"crossref","first-page":"151","DOI":"10.21307\/pjm-2018-029","article-title":"Brucella - Virulence Factors, Pathogenesis and Treatment","volume":"67","author":"Glowacka","year":"2018","journal-title":"Pol. J. Microbiol."},{"key":"10.1016\/j.mimet.2026.107494_bb0055","first-page":"16","article-title":"miR-146b-5p plays a critical role in the regulation of autophagy in increment per Brucella melitensis - infected RAW264.7 cells","volume":"2020","author":"Jiao","year":"2020","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.mimet.2026.107494_bb0060","article-title":"Research progress on complications of Brucellosis","volume":"13","author":"Jin","year":"2023","journal-title":"Front. Cell Infect. Mi."},{"key":"10.1016\/j.mimet.2026.107494_bb0065","doi-asserted-by":"crossref","first-page":"633","DOI":"10.3390\/bioengineering9110633","article-title":"Integrated bioinformatics-based subtractive genomics approach to decipher the therapeutic drug target and its possible intervention against brucellosis","volume":"9","author":"Khan","year":"2022","journal-title":"Bioengineering-Basel"},{"key":"10.1016\/j.mimet.2026.107494_bb0070","doi-asserted-by":"crossref","DOI":"10.3201\/eid2909.230052","article-title":"Global estimate of human brucellosis incidence","volume":"29","author":"Laine","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2026.107494_bb0075","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1128\/JB.00127-21","article-title":"An extracytoplasmic function sigma \/ anti - sigma factor system regulates hypochlorous acid resistance and impacts expression of the type iv secretion system in Brucella melitensis","volume":"203","author":"Li","year":"2021","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2026.107494_bb0080","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1111\/j.1462-5822.2011.01618.x","article-title":"In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system","volume":"13","author":"Marchesini","year":"2011","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.mimet.2026.107494_bb0085","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s12941-017-0182-4","article-title":"Brucella melitensis VirB12 recombinant protein is a potential marker for serodiagnosis of human brucellosis","volume":"16","author":"Mirkalantari","year":"2017","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"10.1016\/j.mimet.2026.107494_bb0090","doi-asserted-by":"crossref","DOI":"10.1080\/07853890.2023.2295398","article-title":"Brucellosis: epidemiology, pathogenesis, diagnosis and treatment-a comprehensive review","volume":"55","author":"Qureshi","year":"2023","journal-title":"Ann. Med."},{"key":"10.1016\/j.mimet.2026.107494_bb0095","doi-asserted-by":"crossref","first-page":"3207","DOI":"10.1128\/IAI.00203-08","article-title":"Inactivation of the Type IV secretion system reduces the Th1 polarization of the immune response Tobrucella abortus infection","volume":"76","author":"Rol\u00e1n","year":"2008","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mimet.2026.107494_bb0100","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/CVI.00374-07","article-title":"VirB12 is a serological marker of Brucella infection in experimental and natural hosts","volume":"15","author":"Rol\u00e1n","year":"2008","journal-title":"Clin. Vac. Immunol.: CVI"},{"key":"10.1016\/j.mimet.2026.107494_bb0105","doi-asserted-by":"crossref","first-page":"e00019","DOI":"10.1128\/MMBR.00021-19","article-title":"Uncovering the hidden credentials of brucella virulence","volume":"85","author":"Roop","year":"2021","journal-title":"Microbiol. Mol. Biol. Rev.: MMBR"},{"key":"10.1016\/j.mimet.2026.107494_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2022.113557","article-title":"A designed peptide-based vaccine to combat Brucella melitensis, B. suis and B. abortus: Harnessing an epitope mapping and immunoinformatics approach","volume":"155","author":"Tarrahimofrad","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.mimet.2026.107494_bb0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.cimid.2020.101462","article-title":"Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis","volume":"70","author":"Tian","year":"2020","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2026.107494_bb0120","article-title":"Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology","volume":"14","author":"Wu","year":"2025","journal-title":"Front. Cell Infect. Mi."},{"key":"10.1016\/j.mimet.2026.107494_bb0125","doi-asserted-by":"crossref","first-page":"13637","DOI":"10.3390\/ijms222413637","article-title":"The VirB system plays a crucial role in brucella intracellular infection","volume":"22","author":"Xiong","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mimet.2026.107494_bb0130","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3389\/fmicb.2025.1684156","article-title":"Establishment of a dual droplet digital PCR method for detecting the Brucella abortus A19-\u0394VirB12 strains","volume":"16","author":"Xue","year":"2025","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2026.107494_bb0135","article-title":"Evaluation and differential diagnosis of a genetic marked brucella vaccine A19\u0394virB12 for cattle","volume":"12","author":"Yang","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.mimet.2026.107494_bb0140","doi-asserted-by":"crossref","first-page":"21","DOI":"10.2460\/ajvr.25.01.0028","article-title":"Development of a VirB12 gene-deleted Brucella abortus A19 strain as a vaccine candidate for differentiating infected from vaccinated animals in cattle and establishment of a differentiating ELISA assay","volume":"86","author":"Ye","year":"2025","journal-title":"Am. J. Vet. Res."},{"key":"10.1016\/j.mimet.2026.107494_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.vas.2025.100530","article-title":"Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-\u2206VirB12","volume":"30","author":"Ye","year":"2025","journal-title":"Vet. Anim. Sci."},{"key":"10.1016\/j.mimet.2026.107494_bb0150","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2021.708008","article-title":"A multi-epitope fusion protein-based p-ELISA method for diagnosing bovine and goat brucellosis","volume":"8","author":"Yin","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.mimet.2026.107494_bb0155","doi-asserted-by":"crossref","first-page":"388","DOI":"10.3390\/clinpract14020030","article-title":"Evolution of laboratory diagnosis of tuberculosis","volume":"14","author":"Zaporojan","year":"2024","journal-title":"Clin. Pract."},{"key":"10.1016\/j.mimet.2026.107494_bb0160","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2174\/1574888X18666230222124529","article-title":"Effector proteins of type IV secretion system: weapons of brucella used to fight against host immunity","volume":"19","author":"Zheng","year":"2024","journal-title":"Curr. Stem. Cell Res. T"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226001065?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226001065?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T12:54:25Z","timestamp":1784552065000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226001065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":32,"alternative-id":["S0167701226001065"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107494","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of a VirB12-based indirect ELISA for differentiating Brucella abortus infection from A19-\u0394VirB12 vaccination","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107494","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107494"}}