{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:26:08Z","timestamp":1790735168686,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.mimet.2026.107568","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:21:53Z","timestamp":1780327313000},"page":"107568","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Detection of viable and VBNC Salmonella in retail meat using optimized PMAxx real-time PCR"],"prefix":"10.1016","volume":"246","author":[{"given":"Hong Thanh","family":"Pham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuyen Huu","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thu Hoang Anh","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anh Ngoc Quynh","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuc Dong","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngan Thi Kim","family":"Phan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lien Thi Xuan","family":"Truong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2026.107568_bb0005","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.mimet.2014.06.003","article-title":"Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar enteritidis","volume":"103","author":"Barbau-Piednoir","year":"2014","journal-title":"J. Microbiol. Methods"},{"issue":"12","key":"10.1016\/j.mimet.2026.107568_bb0010","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.4315\/0362-028X-71.12.2436","article-title":"Rapid detection of Salmonella in foods using real-time PCR","volume":"71","author":"Cheng","year":"2008","journal-title":"J. Food Prot."},{"issue":"4","key":"10.1016\/j.mimet.2026.107568_bb0015","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1007\/s00284-019-01829-y","article-title":"Key factors for removing Bias in viability PCR-based methods: a review","volume":"77","author":"Codony","year":"2020","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.mimet.2026.107568_bb0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2023.106737","article-title":"Understanding the reaction balances behind the viability PCR protocols based on photoreactive dyes","volume":"209","author":"Codony","year":"2023","journal-title":"J. Microbiol. Methods"},{"issue":"2","key":"10.1016\/j.mimet.2026.107568_bb0025","first-page":"117","article-title":"Heat treatment of Bacteria: a simple method of DNA extraction for molecular techniques","volume":"41","author":"Dashti","year":"2009","journal-title":"Kuwait Med. J"},{"issue":"2","key":"10.1016\/j.mimet.2026.107568_bb0030","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1007\/s42770-019-00161-8","article-title":"A novel method for rapid and sensitive detection of viable Escherichia coli cells using UV-induced PMA-coupled quantitative PCR","volume":"51","author":"Deshmukh","year":"2020","journal-title":"Braz. J. Microbiol."},{"issue":"4","key":"10.1016\/j.mimet.2026.107568_bb0035","doi-asserted-by":"crossref","first-page":"311","DOI":"10.26502\/jfsnr.2642-11000057","article-title":"Boiling extraction method vs commercial kits for bacterial DNA isolation from food samples","volume":"3","author":"Dimitrakopoulou","year":"2020","journal-title":"J. Food Sci. Nutr. Res"},{"issue":"12","key":"10.1016\/j.mimet.2026.107568_bb0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0189302","article-title":"Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR","volume":"12","author":"Dinh Thanh","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.mimet.2026.107568_bb0045","author":"European Centre for Disease Prevention and Control"},{"key":"10.1016\/j.mimet.2026.107568_bb0050","author":"European Commission"},{"issue":"10","key":"10.1016\/j.mimet.2026.107568_bb0055","doi-asserted-by":"crossref","first-page":"4281","DOI":"10.1007\/s00253-020-10542-x","article-title":"Methods for detection of viable foodborne pathogens: current state-of-art and future prospects","volume":"104","author":"Foddai","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"7","key":"10.1016\/j.mimet.2026.107568_bb0060","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02566-19","article-title":"Viable but nonculturable Escherichia coli O157: H7 and Salmonella enterica in fresh produce: rapid determination by loop-mediated isothermal amplification coupled with a propidium monoazide treatment","volume":"86","author":"Han","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.mimet.2026.107568_bb0065","first-page":"1","article-title":"Large-scale salmonella outbreak associated with banh mi, Viet Nam, 2024","volume":"15","author":"Ho","year":"2024","journal-title":"West. Pac. Surveill. Response J."},{"key":"10.1016\/j.mimet.2026.107568_bb0070","author":"International standard ISO 6579-1"},{"issue":"11","key":"10.1016\/j.mimet.2026.107568_bb0075","doi-asserted-by":"crossref","DOI":"10.1007\/s13205-022-03371-4","article-title":"Overview of VBNC, a survival strategy for microorganisms","volume":"12","author":"\u0130zg\u00f6rd\u00fc","year":"2022","journal-title":"3 Biotech"},{"issue":"5","key":"10.1016\/j.mimet.2026.107568_bb0080","doi-asserted-by":"crossref","first-page":"2914","DOI":"10.1021\/acs.analchem.4c05593","article-title":"Propidium Monoazide is unreliable for quantitative live\u2013dead molecular assays","volume":"97","author":"Kaur","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.mimet.2026.107568_bb0085","article-title":"Antibiotic and colistin resistance pattern of Salmonella spp. isolated from pediatric patients with diarrhea in the southern region of Vietnam","volume":"65","author":"Le","year":"2025","journal-title":"NMNI"},{"key":"10.1016\/j.mimet.2026.107568_bb0090","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.foodcont.2014.07.011","article-title":"Review of Salmonella detection and identification methods: aspects of rapid emergency response and food safety","volume":"47","author":"Lee","year":"2015","journal-title":"Food Control"},{"key":"10.1016\/j.mimet.2026.107568_bb0095","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.foodcont.2016.09.009","article-title":"Sextuplex PCR combined with immunomagnetic separation and PMA treatment for rapid detection and specific identification of viable Salmonella spp., Salmonella enterica serovars Paratyphi B, Salmonella typhimurium, and Salmonella enteritidis in raw meat","volume":"73","author":"Li","year":"2017","journal-title":"Food Control"},{"issue":"9","key":"10.1016\/j.mimet.2026.107568_bb0100","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1002\/fsn3.2916","article-title":"Simultaneous detection of viable Salmonella spp., Escherichia coli, and Staphylococcus aureus in bird's nest, donkey-hide gelatin, and wolfberry using PMA with multiplex real-time quantitative PCR","volume":"10","author":"Liang","year":"2022","journal-title":"Food Sci. Nutr."},{"key":"10.1016\/j.mimet.2026.107568_bb0105","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2020.107292","article-title":"Rapid and sensitive detection of VBNC Escherichia coli O157: H7 in beef by PMAxx and real-time LAMP","volume":"115","author":"Lv","year":"2020","journal-title":"Food Control"},{"issue":"6","key":"10.1016\/j.mimet.2026.107568_bb0110","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1007\/s00709-024-01971-w","article-title":"Organic extracts from sustainable hybrid poplar hairy root cultures as potential natural antimicrobial and antibiofilm agents","volume":"261","author":"Malik","year":"2024","journal-title":"Protoplasma"},{"issue":"5","key":"10.1016\/j.mimet.2026.107568_bb0115","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1089\/fpd.2020.2891","article-title":"Prevalence, serovar, and antimicrobial resistance of nontyphoidal Salmonella in vegetable, fruit, and water samples in Ho Chi Minh City, Vietnam","volume":"18","author":"Nguyen","year":"2021","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.mimet.2026.107568_bb0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.onehlt.2024.100698","article-title":"A review and meta-analysis of non-typhoidal Salmonella in Vietnam: challenges to the control and antimicrobial resistance traits of a neglected zoonotic pathogen","volume":"18","author":"Nhung","year":"2024","journal-title":"One Health"},{"issue":"2","key":"10.1016\/j.mimet.2026.107568_bb0125","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.mimet.2006.04.015","article-title":"Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells","volume":"67","author":"Nocker","year":"2006","journal-title":"J. Microbiol. Methods"},{"issue":"4","key":"10.1016\/j.mimet.2026.107568_bb0130","doi-asserted-by":"crossref","first-page":"804","DOI":"10.2144\/03344rr02","article-title":"Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5\u2032-nuclease PCR","volume":"34","author":"Nogva","year":"2003","journal-title":"Biotechniques"},{"issue":"14","key":"10.1016\/j.mimet.2026.107568_bb0135","doi-asserted-by":"crossref","DOI":"10.1093\/femsle\/fnab087","article-title":"Behavior of Salmonella enteritidis and shigella flexneri during induction and recovery of the viable but nonculturable state","volume":"368","author":"Oliveira","year":"2021","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mimet.2026.107568_bb0140","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2017.11.011","article-title":"Optimization of PMAxx pretreatment to distinguish between human norovirus with intact and altered capsids in shellfish and sewage samples","volume":"266","author":"Randazzo","year":"2018","journal-title":"Int. J. Food Microbiol."},{"issue":"3","key":"10.1016\/j.mimet.2026.107568_bb0145","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1007\/s42770-020-00261-w","article-title":"Comparison of stress conditions to induce viable but non-cultivable state in Salmonella","volume":"51","author":"Salive","year":"2020","journal-title":"Braz. J. Microbiol."},{"issue":"11530","key":"10.1016\/j.mimet.2026.107568_bb0150","first-page":"1","article-title":"Development of a viability digital PCR protocol for the selective detection and quantification of live Erwinia amylovora cells in cankers","volume":"9","author":"Santander","year":"2019","journal-title":"Sci. Rep."},{"issue":"6","key":"10.1016\/j.mimet.2026.107568_bb0155","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1111\/lam.13468","article-title":"High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157: H7 and Salmonella enterica serovar enteritidis","volume":"72","author":"Song","year":"2021","journal-title":"Lett. Appl. Microbiol."},{"issue":"10","key":"10.1016\/j.mimet.2026.107568_bb0160","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1111\/1750-3841.15459","article-title":"Propidium monoazide for viable Salmonella enterica detection by PCR and LAMP assays in comparison to RNA-based RT-PCR, RT-LAMP, and culture-based assays","volume":"85","author":"Techathuvanan","year":"2020","journal-title":"J. Food Sci."},{"issue":"23","key":"10.1016\/j.mimet.2026.107568_bb0165","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232314708","article-title":"An improved real-time viability PCR assay to detect Salmonella in a culture-independent era","volume":"23","author":"Thilakarathna","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mimet.2026.107568_bb0170","author":"U.S. Centers for Disease Control and Prevention"},{"key":"10.1016\/j.mimet.2026.107568_bb0175","article-title":"Trypan blue exclusion assay, neutral red","author":"Ude","year":"2022","journal-title":"AO\/PI"},{"issue":"5","key":"10.1016\/j.mimet.2026.107568_bb0180","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02653-20","article-title":"A viability quantitative PCR dilemma: are longer amplicons better?","volume":"87","author":"Van Holm","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.mimet.2026.107568_bb0185","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.jfda.2015.03.002","article-title":"Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide","volume":"23","author":"Wang","year":"2015","journal-title":"J. Food Drug Anal."},{"key":"10.1016\/j.mimet.2026.107568_bb0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2019.113363","article-title":"Monitoring long-term DNA storage via absolute copy number quantification by ddPCR","volume":"583","author":"Wang","year":"2019","journal-title":"Anal. Biochem."},{"issue":"10","key":"10.1016\/j.mimet.2026.107568_bb0195","doi-asserted-by":"crossref","DOI":"10.1093\/lambio\/ovad120","article-title":"A comparative analysis of quantitative detection methods for viable food-borne pathogens using RT-qPCR and PMA-qPCR","volume":"76","author":"Wang","year":"2023","journal-title":"Lett. Appl. Microbiol."},{"issue":"8","key":"10.1016\/j.mimet.2026.107568_bb0200","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1007\/s12275-020-0084-6","article-title":"Simultaneous detection of Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7 in environmental water using PMA combined with mPCR","volume":"58","author":"Xie","year":"2020","journal-title":"J. Microbiol."},{"issue":"7","key":"10.1016\/j.mimet.2026.107568_bb0205","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1139\/cjm-2018-0547","article-title":"Propidium monoazide real-time PCR amplification for viable Salmonella species and Salmonella Heidelberg in pork","volume":"65","author":"Zhai","year":"2019","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.mimet.2026.107568_bb0210","article-title":"Development of PMAxxTM-based qPCR for the quantification of viable and non-viable load of Salmonella from poultry environment","volume":"11","author":"Zhang","year":"2020","journal-title":"Front. Microbiol."},{"issue":"10","key":"10.1016\/j.mimet.2026.107568_bb0215","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1007\/s44211-023-00384-8","article-title":"Advances in detection methods for viable Salmonella spp.: current applications and challenges","volume":"39","author":"Zhuang","year":"2023","journal-title":"Anal. Sci."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226001806?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226001806?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T11:42:05Z","timestamp":1784202125000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226001806"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":43,"alternative-id":["S0167701226001806"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107568","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of viable and VBNC Salmonella in retail meat using optimized PMAxx real-time PCR","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107568","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107568"}}