{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T02:12:58Z","timestamp":1785031978587,"version":"3.55.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.mimet.2026.107585","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:05:00Z","timestamp":1781222700000},"page":"107585","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Extract-free one-pot ambient RPA-CRISPR detection of Plasmodium in whole blood"],"prefix":"10.1016","volume":"247","author":[{"given":"Jipei","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.mimet.2026.107585_bb0005","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.molcel.2014.03.011","article-title":"CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity","volume":"54","author":"Barrangou","year":"2014","journal-title":"Mol. Cell"},{"issue":"2","key":"10.1016\/j.mimet.2026.107585_bb0010","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25020695","article-title":"The laboratory diagnosis of malaria: a focus on the diagnostic assays in non-endemic areas","volume":"25","author":"Calderaro","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"33","key":"10.1016\/j.mimet.2026.107585_bb0015","doi-asserted-by":"crossref","first-page":"18355","DOI":"10.1021\/acs.analchem.5c03865","article-title":"Ultrasensitive detection of nucleic acid and protein at ambient temperature by using an engineered CRISPR-Cas12a-based digital assay","volume":"97","author":"Cheng","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.mimet.2026.107585_bb0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2021.103415","article-title":"A novel CRISPR-based malaria diagnostic capable of plasmodium detection, species differentiation, and drug-resistance genotyping","volume":"68","author":"Cunningham","year":"2021","journal-title":"EBioMedicine"},{"issue":"13","key":"10.1016\/j.mimet.2026.107585_bb0025","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1056\/NEJMra2405313","article-title":"Malaria","volume":"392","author":"Daily","year":"2025","journal-title":"N. Engl. J. Med."},{"issue":"2","key":"10.1016\/j.mimet.2026.107585_bb0030","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.parepi.2017.04.001","article-title":"A sensitive species-specific reverse transcription real-time PCR method for detection of Plasmodium falciparum and plasmodium vivax","volume":"2","author":"Gavina","year":"2017","journal-title":"Parasite Epidemiol. Control"},{"issue":"6336","key":"10.1016\/j.mimet.2026.107585_bb0035","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.mimet.2026.107585_bb0040","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2025.1640938","article-title":"Research progress on the application of RPA-CRISPR\/Cas12a in the rapid visual detection of pathogenic microorganisms","volume":"15","author":"Ji","year":"2025","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0045","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1186\/s13071-025-06779-y","article-title":"Off-grid field-deployable molecular diagnostic platform for malaria surveillance","volume":"18","author":"Kona","year":"2025","journal-title":"Parasit. Vectors"},{"issue":"41","key":"10.1016\/j.mimet.2026.107585_bb0050","doi-asserted-by":"crossref","first-page":"25722","DOI":"10.1073\/pnas.2010196117","article-title":"Ultrasensitive CRISPR-based diagnostic for field-applicable detection of Plasmodium species in symptomatic and asymptomatic malaria","volume":"117","author":"Lee","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"23","key":"10.1016\/j.mimet.2026.107585_bb0055","doi-asserted-by":"crossref","DOI":"10.3390\/jcm11236923","article-title":"Profiling plasma cytokines by a CRISPR-ELISA assay for early detection of lung Cancer","volume":"11","author":"Li","year":"2022","journal-title":"J. Clin. Med."},{"issue":"3","key":"10.1016\/j.mimet.2026.107585_bb0060","first-page":"425","article-title":"SARS-CoV-2 and its omicron variants detection with RT-RPA -CRISPR\/Cas13a-based method at room temperature","volume":"12","author":"Li","year":"2023","journal-title":"Rep. Biochem. Mol. Biol."},{"key":"10.1016\/j.mimet.2026.107585_bb0065","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2019.113351","article-title":"Direct genotyping from whole blood using alkaline polyethylene glycol","volume":"582","author":"Liu","year":"2019","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0070","doi-asserted-by":"crossref","first-page":"9142","DOI":"10.1038\/s41467-025-64205-4","article-title":"CRISPR anti-tag-mediated room-temperature RNA detection using CRISPR\/Cas13a","volume":"16","author":"Moon","year":"2025","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0075","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1038\/s41598-025-86373-5","article-title":"Room temperature CRISPR diagnostics for low-resource settings","volume":"15","author":"Nalefski","year":"2025","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0080","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-025-97259-x","article-title":"Performance and challenges of malaria rapid diagnostic tests in endemic regions of Africa","volume":"15","author":"Ojeniyi","year":"2025","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0085","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s12936-023-04506-5","article-title":"Accuracy of diagnosis among clinical malaria patients: comparing microscopy, RDT and a highly sensitive quantitative PCR looking at the implications for submicroscopic infections","volume":"22","author":"Opoku Afriyie","year":"2023","journal-title":"Malar. J."},{"issue":"6","key":"10.1016\/j.mimet.2026.107585_bb0090","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/S1473-3099(24)00139-7","article-title":"Progress towards safer and more effective radical cure of Plasmodium vivax","volume":"24","author":"Price","year":"2024","journal-title":"Lancet Infect. Dis."},{"issue":"12","key":"10.1016\/j.mimet.2026.107585_bb0095","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1128\/JCM.42.12.5636-5643.2004","article-title":"Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays","volume":"42","author":"Rougemont","year":"2004","journal-title":"J. Clin. Microbiol."},{"issue":"7","key":"10.1016\/j.mimet.2026.107585_bb0100","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"issue":"12","key":"10.1016\/j.mimet.2026.107585_bb0105","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1016\/j.tranon.2019.08.011","article-title":"A CRISPR test for detection of circulating nuclei acids","volume":"12","author":"Tsou","year":"2019","journal-title":"Transl. Oncol."},{"issue":"2","key":"10.1016\/j.mimet.2026.107585_bb0110","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics10020114","article-title":"A CRISPR test for rapidly and sensitively detecting circulating EGFR mutations","volume":"10","author":"Tsou","year":"2020","journal-title":"Diagnostics"},{"issue":"3","key":"10.1016\/j.mimet.2026.107585_bb0115","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines9030239","article-title":"Rapid and sensitive detection of SARS-CoV-2 using clustered regularly interspaced short palindromic repeats","volume":"9","author":"Tsou","year":"2021","journal-title":"Biomedicines"},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0120","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s12936-023-04496-4","article-title":"Laboratory evaluation of the miniature direct-on-blood PCR nucleic acid lateral flow immunoassay (mini-dbPCR-NALFIA), a simplified molecular diagnostic test for Plasmodium","volume":"22","author":"van Dijk","year":"2023","journal-title":"Malar. J."},{"issue":"9596","key":"10.1016\/j.mimet.2026.107585_bb0125","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1016\/S0140-6736(07)61602-X","article-title":"The strengthening the reporting of observational studies in epidemiology (STROBE) statement: guidelines for reporting observational studies","volume":"370","author":"von Elm","year":"2007","journal-title":"Lancet"},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0130","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1186\/s13071-023-05868-0","article-title":"CRISPR\/Cas12a combined with RPA for detection of T. gondii in mouse whole blood","volume":"16","author":"Wang","year":"2023","journal-title":"Parasit. Vectors"},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0135","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01550-25","article-title":"An RPA-CRISPR\/Cas12a-based rapid and sensitive nucleic acid method for detection of toxoplasma gondii in tissue and blood samples","volume":"14","author":"Wang","year":"2026","journal-title":"Microbiol. Spectrum"},{"issue":"4","key":"10.1016\/j.mimet.2026.107585_bb0140","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1373\/clinchem.2018.299347","article-title":"Room temperature detection of plasma Epstein-Barr virus DNA with CRISPR-Cas13","volume":"65","author":"Wu","year":"2019","journal-title":"Clin. Chem."},{"issue":"1","key":"10.1016\/j.mimet.2026.107585_bb0145","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics11010125","article-title":"Sensitive detection of KRAS mutations by clustered regularly interspaced short palindromic repeats","volume":"11","author":"Zhou","year":"2021","journal-title":"Diagnostics"},{"issue":"5","key":"10.1016\/j.mimet.2026.107585_bb0150","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics11050823","article-title":"Detection and differentiation of SARS-CoV-2, influenza, and respiratory syncytial viruses by CRISPR","volume":"11","author":"Zhou","year":"2021","journal-title":"Diagnostics"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226001971?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226001971?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T16:33:09Z","timestamp":1783787589000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226001971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":30,"alternative-id":["S0167701226001971"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107585","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Extract-free one-pot ambient RPA-CRISPR detection of Plasmodium in whole blood","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107585","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107585"}}