{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:51:27Z","timestamp":1785912687076,"version":"3.56.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["226817\/Z\/22\/Z"],"award-info":[{"award-number":["226817\/Z\/22\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.mimet.2026.107591","type":"journal-article","created":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T17:35:09Z","timestamp":1781458509000},"page":"107591","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Non-uniform growth probability of Mycobacterium tuberculosis across inoculum densities partially restored by culture filtrate: Implications for supplemented growth assays"],"prefix":"10.1016","volume":"247","author":[{"given":"Ryan","family":"Dinkele","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ditshego","family":"Ralefeta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atica","family":"Moosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Digby F.","family":"Warner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Gessner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2026.107591_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.tube.2020.101945","article-title":"Differentially culturable tubercle bacteria dynamics during standard anti-tuberculosis treatment: a prospective cohort study","volume":"124","author":"Almeida J\u00fanior","year":"2020","journal-title":"Tuberculosis"},{"issue":"6","key":"10.1016\/j.mimet.2026.107591_bb0010","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.gde.2011.10.001","article-title":"Persistence: mechanisms for triggering and enhancing phenotypic variability","volume":"21","author":"Balaban","year":"2011","journal-title":"Curr. Opin. Genet. Dev."},{"key":"10.1016\/j.mimet.2026.107591_bb0015","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0065-2911(08)60166-6","article-title":"Bacterial viability and culturability","volume":"41","author":"Barer","year":"1999","journal-title":"Adv. Microb. Physiol."},{"issue":"1","key":"10.1016\/j.mimet.2026.107591_bb0020","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98176-5","article-title":"Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria","volume":"11","author":"Barr","year":"2021","journal-title":"Sci. Rep."},{"issue":"5","key":"10.1016\/j.mimet.2026.107591_bb0025","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/0043-1354(93)90143-6","article-title":"The \u201cmost probable number\u201d estimate and its confidence limits","volume":"27","author":"Beliaeff","year":"1993","journal-title":"Water Res."},{"key":"10.1016\/j.mimet.2026.107591_bb0030","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2020.00443","article-title":"Investigating non-sterilizing cure in TB patients at the end of successful anti-TB therapy [original research]","volume":"10","author":"Beltran","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.mimet.2026.107591_bb0035","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1065893","article-title":"Culture filtrate supplementation can be used to improve mycobacterium tuberculosis culture positivity for spinal tuberculosis diagnosis","volume":"12","author":"Beltran","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.mimet.2026.107591_bb0040","doi-asserted-by":"crossref","DOI":"10.1164\/rccm.201604-0769OC","article-title":"Detection and quantification of differentially Culturable tubercle Bacteria in sputum from patients with tuberculosis","author":"Chengalroyen","year":"2016","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.mimet.2026.107591_bb0045","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2020.582416","article-title":"Biological profiling enables rapid mechanistic classification of phenotypic screening hits and identification of KatG activation-dependent pyridine Carboxamide prodrugs with activity against mycobacterium tuberculosis [original research]","author":"Chengalroyen","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"12","key":"10.1016\/j.mimet.2026.107591_bb0050","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1038\/s41564-024-01846-z","article-title":"Single-cell imaging of the mycobacterium tuberculosis cell cycle reveals linear and heterogenous growth","volume":"9","author":"Chung","year":"2024","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.mimet.2026.107591_bb0055","series-title":"Statistical Models in S","first-page":"309","article-title":"Local regression models","author":"Cleveland","year":"2017"},{"issue":"3","key":"10.1016\/j.mimet.2026.107591_bb0060","doi-asserted-by":"crossref","first-page":"642","DOI":"10.2136\/sssaj1985.03615995004900030023x","article-title":"Evaluation of the Most probable number method for enumerating denitrifying Bacteria","volume":"49","author":"Davidson","year":"1985","journal-title":"Soil Sci. Soc. Am. J."},{"key":"10.1016\/j.mimet.2026.107591_bb0065","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.60083","article-title":"Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes","volume":"9","author":"de Wet","year":"2020","journal-title":"eLife"},{"issue":"2","key":"10.1016\/j.mimet.2026.107591_bb0070","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009262","article-title":"Capture and visualization of live mycobacterium tuberculosis bacilli from tuberculosis patient bioaerosols","volume":"17","author":"Dinkele","year":"2021","journal-title":"PLoS Pathog."},{"issue":"1","key":"10.1016\/j.mimet.2026.107591_bb0075","first-page":"1","article-title":"Fitting linear mixed-effects models using lme4","volume":"67","author":"Douglas","year":"2015","journal-title":"J. Stat. Softw."},{"issue":"7","key":"10.1016\/j.mimet.2026.107591_bb0080","doi-asserted-by":"crossref","DOI":"10.1038\/nmicrobiol.2016.77","article-title":"MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis","volume":"1","author":"Ducret","year":"2016","journal-title":"Nat. Microbiol."},{"issue":"10","key":"10.1016\/j.mimet.2026.107591_bb0085","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/s41579-024-01025-1","article-title":"Drug-resistant tuberculosis: a persistent global health concern","volume":"22","author":"Farhat","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.mimet.2026.107591_bb0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2026.107528","article-title":"Decoding the MPN principle: clarifying the logical structure of most probable number estimation","volume":"246","author":"Gunawardane","year":"2026","journal-title":"J. Microbiol. Methods"},{"issue":"12","key":"10.1016\/j.mimet.2026.107591_bb0095","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1128\/aem.54.12.2996-3002.1988","article-title":"Test of the validity of the Poisson assumption for analysis of most-probable-number results","volume":"54","author":"Haas","year":"1988","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.mimet.2026.107591_bb0100","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1111\/j.1365-2958.2007.05945.x","article-title":"A partner for the resuscitation-promoting factors of mycobacterium tuberculosis","volume":"66","author":"Hett","year":"2007","journal-title":"Mol. Microbiol."},{"issue":"2","key":"10.1016\/j.mimet.2026.107591_bb0105","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000001","article-title":"A mycobacterial enzyme essential for cell division synergizes with resuscitation-promoting factor","volume":"4","author":"Hett","year":"2008","journal-title":"PLoS Pathog."},{"issue":"3","key":"10.1016\/j.mimet.2026.107591_bb0110","doi-asserted-by":"crossref","first-page":"e00221","DOI":"10.1128\/cmr.00227-21","article-title":"Tuberculosis treatment monitoring and outcome measures: new interest and new strategies","volume":"35","author":"Heyckendorf","year":"2022","journal-title":"Clin. Microbiol. Rev."},{"issue":"4","key":"10.1016\/j.mimet.2026.107591_bb0115","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1046\/j.1365-2672.2000.01020.x","article-title":"The physiology and pathogenicity of mycobacterium tuberculosis grown under controlled conditions in a defined medium","volume":"88","author":"James","year":"2000","journal-title":"J. Appl. Microbiol."},{"issue":"430","key":"10.1016\/j.mimet.2026.107591_bb0120","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aam6310","article-title":"Rapid detection of mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe","volume":"10","author":"Kamariza","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.mimet.2026.107591_bb0125","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.pbiomolbio.2019.10.001","article-title":"Tuberculosis control in crisis-causes and solutions","volume":"152","author":"Kaplan","year":"2020","journal-title":"Prog. Biophys. Mol. Biol."},{"issue":"7","key":"10.1016\/j.mimet.2026.107591_bb0130","doi-asserted-by":"crossref","first-page":"O418","DOI":"10.1111\/1469-0691.12441","article-title":"Early stationary phase culture supernatant accelerates growth of sputum cultures collected after initiation of anti-tuberculosis treatment","volume":"20","author":"Kolwijck","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"issue":"1","key":"10.1016\/j.mimet.2026.107591_bb0135","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1128\/CMR.00014-14","article-title":"The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota","volume":"28","author":"Lagier","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.mimet.2026.107591_bb0140","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.943545","article-title":"Drug resistant tuberculosis: implications for transmission, diagnosis, and disease management [review]","author":"Liebenberg","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2026.107591_bb0145","doi-asserted-by":"crossref","first-page":"3588","DOI":"10.1038\/s41598-024-85092-7","article-title":"Drug-induced differential culturability in diverse strains of mycobacterium tuberculosis","volume":"15","author":"March","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mimet.2026.107591_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.tube.2021.102103","article-title":"Supplementation of sputum cultures with culture filtrate to detect tuberculosis in a cross-sectional study of HIV-infected individuals","volume":"129","author":"McIvor","year":"2021","journal-title":"Tuberculosis"},{"issue":"1","key":"10.1016\/j.mimet.2026.107591_bb0155","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1365-2958.2005.04930.x","article-title":"Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation","volume":"59","author":"Mukamolova","year":"2006","journal-title":"Mol. Microbiol."},{"issue":"2","key":"10.1016\/j.mimet.2026.107591_bb0160","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1164\/rccm.200905-0661OC","article-title":"Resuscitation-promoting factors reveal an occult population of tubercle Bacilli in sputum","volume":"181","author":"Mukamolova","year":"2010","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"suppl_7","key":"10.1016\/j.mimet.2026.107591_bb0165","doi-asserted-by":"crossref","first-page":"S702","DOI":"10.1093\/infdis\/jix335","article-title":"The south African tuberculosis care Cascade: estimated losses and methodological challenges","volume":"216","author":"Naidoo","year":"2017","journal-title":"J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mimet.2026.107591_bb0170","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s10482-012-9784-1","article-title":"Peptidoglycan fragments stimulate resuscitation of \u201cnon-culturable\u201d mycobacteria","volume":"103","author":"Nikitushkin","year":"2013","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"13","key":"10.1016\/j.mimet.2026.107591_bb0175","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1111\/febs.13292","article-title":"A product of RpfB and RipA joint enzymatic action promotes the resuscitation of dormant mycobacteria","volume":"282","author":"Nikitushkin","year":"2015","journal-title":"FEBS J."},{"issue":"5","key":"10.1016\/j.mimet.2026.107591_bb0180","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1038\/s41564-023-01365-3","article-title":"Transforming tuberculosis diagnosis","volume":"8","author":"Pai","year":"2023","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.mimet.2026.107591_bb0185","article-title":"Differentially culturable tubercle bacteria as a measure of tuberculosis treatment response","volume":"12","author":"Peters","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.mimet.2026.107591_bb0190","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3389\/fpubh.2014.00103","article-title":"Current perspectives on viable but non-Culturable (VBNC) pathogenic Bacteria","volume":"2","author":"Ramamurthy","year":"2014","journal-title":"Front. Public Health"},{"key":"10.1016\/j.mimet.2026.107591_bb0195","author":"RCoreTeam"},{"issue":"24","key":"10.1016\/j.mimet.2026.107591_bb0200","doi-asserted-by":"crossref","first-page":"E4832","DOI":"10.1073\/pnas.1705385114","article-title":"Rifamycin action on RNA polymerase in antibiotic-tolerant mycobacterium tuberculosis results in differentially detectable populations","volume":"114","author":"Saito","year":"2017","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"5","key":"10.1016\/j.mimet.2026.107591_bb0205","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1099\/00221287-148-5-1581","article-title":"Formation and resuscitation of \u2018non-culturable\u2019 cells of Rhodococcus rhodochrous and mycobacterium tuberculosis in prolonged stationary phase","volume":"148","author":"Shleeva","year":"2002","journal-title":"Microbiology"},{"key":"10.1016\/j.mimet.2026.107591_bb0210","series-title":"In","first-page":"1","article-title":"The next generation of drug resistant tuberculosis drug design","author":"Singh","year":"2025"},{"issue":"31","key":"10.1016\/j.mimet.2026.107591_bb0215","doi-asserted-by":"crossref","first-page":"18744","DOI":"10.1073\/pnas.2002738117","article-title":"Morphological profiling of tubercle bacilli identifies drug pathways of action","volume":"117","author":"Smith","year":"2020","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mimet.2026.107591_bb0220","series-title":"Global tuberculosis report 2024","author":"WHO","year":"2024"},{"issue":"4","key":"10.1016\/j.mimet.2026.107591_bb0225","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1111\/j.1365-2672.2008.03850.x","article-title":"Effect of recombinant Rv1009 protein on promoting the growth of mycobacterium tuberculosis","volume":"105","author":"Wu","year":"2008","journal-title":"J. Appl. Microbiol."},{"issue":"6","key":"10.1016\/j.mimet.2026.107591_bb0230","doi-asserted-by":"crossref","first-page":"e02701","DOI":"10.1128\/mbio.02701-22","article-title":"Transcriptional Biomarkers of Differentially Detectable Mycobacterium tuberculosis in Patient Sputum","volume":"13","author":"Zainabadi","year":"2022","journal-title":"mBio"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226002034?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226002034?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:24:31Z","timestamp":1785911071000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226002034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":46,"alternative-id":["S0167701226002034"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107591","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Non-uniform growth probability of Mycobacterium tuberculosis across inoculum densities partially restored by culture filtrate: Implications for supplemented growth assays","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107591","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107591"}}