{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:29:05Z","timestamp":1786116545507,"version":"build-2736575974"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100023448","name":"Robert Koch Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100023448","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.mimet.2026.107609","type":"journal-article","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T14:18:44Z","timestamp":1782656324000},"page":"107609","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Method for needle-free release of pathogens from host cells using reusable chromatography components"],"prefix":"10.1016","volume":"247","author":[{"given":"Frank","family":"Seeber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharareh Salehi","family":"Hossainy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph-Martin","family":"Ufermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ojo-Ajogu","family":"Akuh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Warschkau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefania Delgado","family":"Betancourt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2026.107609_bb0005","doi-asserted-by":"crossref","first-page":"3A.5.1","DOI":"10.1002\/9780471729259.mc03a05s11","article-title":"Laboratory maintenance of Rickettsia rickettsii","volume":"11","author":"Ammerman","year":"2008","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0010","first-page":"377","article-title":"Impact of needle selection on survival of muscle-derived cells when used for laryngeal injections","volume":"14","author":"Awonusi","year":"2023","journal-title":"J. Cell. Sci. Ther."},{"key":"10.1016\/j.mimet.2026.107609_bb0015","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1046\/j.1462-5822.2002.00183.x","article-title":"Association of a macrophage galactoside-binding protein with Mycobacterium-containing phagosomes","volume":"4","author":"Beatty","year":"2002","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0020","first-page":"Unit 1A.1","article-title":"Biosafety: guidelines for working with pathogenic and infectious microorganisms","author":"Burnett","year":"2009","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0025","series-title":"Biological Safety: Principles and Practices","first-page":"59","article-title":"Laboratory-Associated Infections","author":"Byers","year":"2017"},{"key":"10.1016\/j.mimet.2026.107609_bb0030","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1084\/jem.176.5.1311","article-title":"Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions","volume":"176","author":"Carlson","year":"1992","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.mimet.2026.107609_bb0035","doi-asserted-by":"crossref","unstructured":"de Ara\u00fajo, M.E.G., Lamberti, G., Huber, L.A., 2015. Homogenization of mammalian cells. Cold Spring Harb. Protoc. 2015, 1009\u20131012.","DOI":"10.1101\/pdb.prot083436"},{"key":"10.1016\/j.mimet.2026.107609_bb0040","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00673-18","article-title":"Stress-induced proliferation and cell cycle plasticity of intracellular Trypanosoma cruzi amastigotes","volume":"9","author":"Dumoulin","year":"2018","journal-title":"MBio"},{"key":"10.1016\/j.mimet.2026.107609_bb0045","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1177\/014107680409700705","article-title":"Needle-stick injuries in the National Health Service: a culture of silence","volume":"97","author":"Elmiyeh","year":"2004","journal-title":"J. Royal Soc. Med."},{"key":"10.1016\/j.mimet.2026.107609_bb0050","series-title":"Biological Safety: Principles and Practices","first-page":"105","article-title":"Protozoa and Helminths","author":"Herwaldt","year":"2017"},{"key":"10.1016\/j.mimet.2026.107609_bb0055","series-title":"Toxoplasma Gondii (Third Edition)","first-page":"897","article-title":"Chapter 20 - genetic manipulation of Toxoplasma gondii","author":"Jacot","year":"2020"},{"key":"10.1016\/j.mimet.2026.107609_bb0060","doi-asserted-by":"crossref","DOI":"10.1002\/cpmc.26","article-title":"Toxoplasma gondii: laboratory maintenance and growth","volume":"44","author":"Khan","year":"2017","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0065","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/S0091-679X(08)61571-X","article-title":"Cell culture contamination: sources, consequences, prevention, and elimination","volume":"57","author":"Lincoln","year":"1998","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.mimet.2026.107609_bb0070","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/978-1-4939-9857-9_15","article-title":"Toxoplasma gondii: bradyzoite differentiation in vitro and in vivo","volume":"2071","author":"Mayoral","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.mimet.2026.107609_bb0075","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaerosci.2023.106137","article-title":"Aerosol generation by liquid jet impingement onto a solid surface","volume":"169","author":"Mbaye","year":"2023","journal-title":"J. Aerosol Sci."},{"key":"10.1016\/j.mimet.2026.107609_bb0080","doi-asserted-by":"crossref","first-page":"L031601","DOI":"10.1103\/PhysRevFluids.6.L031601","article-title":"Aerosol agitation: quantifying the hydrodynamic stressors on particulates encapsulated in small droplets","volume":"6","author":"McRae","year":"2021","journal-title":"Phys. Rev. Fluids."},{"key":"10.1016\/j.mimet.2026.107609_bb0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/2332-0877.1000352","article-title":"Occupationally acquired P. falciparum malaria","volume":"06","author":"Mickiene","year":"2018","journal-title":"J. Infect. Dis. Ther."},{"key":"10.1016\/j.mimet.2026.107609_bb0090","doi-asserted-by":"crossref","DOI":"10.1115\/1.4071455","article-title":"Excessive shear rate, not shear stress, influences cell mechanical damage in small-bore needle injections","volume":"148","author":"Morgan","year":"2026","journal-title":"J. Biomech. Eng."},{"key":"10.1016\/j.mimet.2026.107609_bb0095","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.surge.2023.10.009","article-title":"Time to consider blunt needles for implant surgery? A systematic review and meta-analysis shows that blunt suture needles reduce glove perforation","volume":"22","author":"O'Donnell","year":"2024","journal-title":"Surgeon"},{"key":"10.1016\/j.mimet.2026.107609_bb0100","first-page":"Unit 19.15","article-title":"Animal models of mycobacteria infection","author":"Orme","year":"2001","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.mimet.2026.107609_bb0105","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.3390\/ijerph15122697","article-title":"Improved biosafety and biosecurity measures and\/or strategies to tackle laboratory-acquired infections and related risks","volume":"15","author":"Peng","year":"2018","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"10.1016\/j.mimet.2026.107609_bb0110","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1177\/153567601401900303","article-title":"Quantification of microbial aerosol generation during standard laboratory procedures","volume":"19","author":"Pottage","year":"2014","journal-title":"Appl. Biosaf."},{"key":"10.1016\/j.mimet.2026.107609_bb0115","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1089\/apb.2021.0038","article-title":"Microbial aerosols generated from standard microbiological laboratory procedures","volume":"27","author":"Pottage","year":"2022","journal-title":"Appl. Biosaf."},{"key":"10.1016\/j.mimet.2026.107609_bb0120","doi-asserted-by":"crossref","first-page":"1728709","DOI":"10.3389\/fpubh.2025.1728709","article-title":"Preventing laboratory-acquired Toxoplasma gondii infection: literature review and case-based post-exposure prophylaxis proposal","volume":"13","author":"P\u00f3voas","year":"2025","journal-title":"Front. Public Health"},{"key":"10.1016\/j.mimet.2026.107609_bb0125","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1111\/cmi.12311","article-title":"Cell-to-cell transfer of Leishmania amazonensis amastigotes is mediated by immunomodulatory LAMP-rich parasitophorous extrusions","volume":"16","author":"Real","year":"2014","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0130","article-title":"Devices for preventing percutaneous exposure injuries caused by needles in healthcare personnel","volume":"11","author":"Reddy","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"10.1016\/j.mimet.2026.107609_bb0135","doi-asserted-by":"crossref","first-page":"6C. 1.1","DOI":"10.1002\/9780471729259.mc06c01s15","article-title":"Laboratory maintenance of Coxiella burnetii","volume":"15","author":"Samuel","year":"2009","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0140","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0378-1119(95)00786-5","article-title":"Escherichia coli beta-galactosidase as an in vitro and in vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite Toxoplasma gondii","volume":"169","author":"Seeber","year":"1996","journal-title":"Gene"},{"key":"10.1016\/j.mimet.2026.107609_bb0145","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1115\/1.1992528","article-title":"Experimental investigation into the deep penetration of soft solids by sharp and blunt punches, with application to the piercing of skin","volume":"127","author":"Shergold","year":"2005","journal-title":"J. Biomech. Eng."},{"key":"10.1016\/j.mimet.2026.107609_bb0150","series-title":"Biological Safety: Principles and Practices","first-page":"411","article-title":"Aerosols in the Microbiology Laboratory","author":"Shieber","year":"2017"},{"key":"10.1016\/j.mimet.2026.107609_bb0155","doi-asserted-by":"crossref","first-page":"762","DOI":"10.3855\/jidc.9428","article-title":"Laboratory associated zoonotic parasitic infections: a review of main agents and biosecurity measures","volume":"17","author":"Sini","year":"2023","journal-title":"J. Infect. Dev. Ctries."},{"key":"10.1016\/j.mimet.2026.107609_bb0160","article-title":"Animal models for Toxoplasma gondii infection","volume":"3","author":"Subauste","year":"2023","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0165","series-title":"Effectiveness of Measures to Prevent Needlestick Injuries among Employees in Health Professions","first-page":"118","author":"Sulsky","year":"2006"},{"key":"10.1016\/j.mimet.2026.107609_bb0170","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.3201\/eid1010.040277","article-title":"Occupational malaria following needlestick injury","volume":"10","author":"Tarantola","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mimet.2026.107609_bb0175","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ajic.2004.11.011","article-title":"Infection risks following accidental exposure to blood or body fluids in health care workers: a review of pathogens transmitted in published cases","volume":"34","author":"Tarantola","year":"2006","journal-title":"Am. J. Infect. Control"},{"key":"10.1016\/j.mimet.2026.107609_bb0180","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1007\/s00436-002-0695-3","article-title":"A simple and reproducible method to obtain large numbers of axenic amastigotes of different Leishmania species","volume":"88","author":"Teixeira","year":"2002","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.mimet.2026.107609_bb0185","doi-asserted-by":"crossref","first-page":"9194","DOI":"10.1038\/s41598-018-27568-x","article-title":"Ex vivo biomechanical characterization of syringe-needle ejections for intracerebral cell delivery","volume":"8","author":"Wahlberg","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mimet.2026.107609_bb0190","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/978-1-4939-9857-9_5","article-title":"Assays for monitoring Toxoplasma gondii infectivity in the laboratory mouse","volume":"2071","author":"Wang","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.mimet.2026.107609_bb0195","doi-asserted-by":"crossref","DOI":"10.1002\/cpmc.30","article-title":"Purification Toxoplasma gondii tissue cysts using percoll gradients","volume":"45","author":"Watts","year":"2017","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.mimet.2026.107609_bb0200","series-title":"WHO Guideline on the Use of Safety-Engineered Syringes for Intramuscular, Intradermal and Subcutaneous Injections in Health Care Settings","author":"World Health Organization","year":"2016"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226002216?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226002216?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T10:50:02Z","timestamp":1786013402000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226002216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":40,"alternative-id":["S0167701226002216"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107609","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Method for needle-free release of pathogens from host cells using reusable chromatography components","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107609","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107609"}}