{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:11:19Z","timestamp":1789049479932,"version":"build-2803163510"},"reference-count":81,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006261","name":"Taif University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.mimet.2026.107642","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T23:30:39Z","timestamp":1786145439000},"page":"107642","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Solvent extraction and analytical characterization of polyhydroxyalkanoate inclusions produced by the microeukaryote Paramecium jenningsi"],"prefix":"10.1016","volume":"249","author":[{"given":"Shahid","family":"Nawaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itrat","family":"Zahra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Liaqat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bushra","family":"Muneer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghulam","family":"Mustafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayesha","family":"Arshad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usama","family":"Shabbir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awais","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuba","family":"Arooj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibtissem","family":"Louiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amor","family":"Hedfi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel Ben","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul Rauf","family":"Shakoori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah Rauf","family":"Shakoori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2026.107642_bb0005","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1002\/jctb.5572","article-title":"Purification and characterization of polyhydroxyalkanoate (PHA) from a Bacillus megaterium strain using various dehydration techniques","volume":"93","author":"Akdo\u011fan","year":"2018","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"10.1016\/j.mimet.2026.107642_bb0010","first-page":"180","article-title":"Assessment of groundwater and surface water quality within agricultural lands: a case study from the Novoanninsky district, Volgograd region, Russia","volume":"14","author":"Alexandrovna","year":"2025","journal-title":"Int. J. Agric. Biosci."},{"key":"10.1016\/j.mimet.2026.107642_bb0015","first-page":"42","article-title":"Public awareness and attitudes toward environmental pollution: a survey study in district Lahore, Punjab-Pakistan","volume":"4","author":"Ali","year":"2025","journal-title":"Sci. Soc. Insights"},{"key":"10.1016\/j.mimet.2026.107642_bb0020","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.ijbiomac.2016.04.069","article-title":"Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: a review of recent advancements","volume":"89","author":"Anjum","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107642_bb0025","first-page":"1","article-title":"Global sustainable agriculture: challenges, strategies, and future pathways","volume":"4","author":"Arif","year":"2025","journal-title":"Sci. Soc. Insights"},{"key":"10.1016\/j.mimet.2026.107642_bb0030","doi-asserted-by":"crossref","DOI":"10.1080\/02757540.2025.2569319","article-title":"Growth optimization and biochemical adaptation of Paramecium jenningsi in the bioremediation of Dianix yellow brown azo dye","author":"Arooj","year":"2026","journal-title":"J. Chem. Ecol."},{"key":"10.1016\/j.mimet.2026.107642_bb0035","doi-asserted-by":"crossref","DOI":"10.1007\/s12649-026-03591-2","article-title":"Bioconversion of sugarcane bagasse into polyhydroxyalkanoates by Paramecium caudatum: a protozoan cell factory for lignocellulosic waste valorization","author":"Arooj","year":"2026","journal-title":"Waste Biomass Valor."},{"key":"10.1016\/j.mimet.2026.107642_bb0040","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s11756-025-02133-w","article-title":"Harnessing Paramecium primaurelia for polyhydroxyalkanoate production: optimization of carbon sources for sustainable bioplastic synthesis","volume":"81","author":"Arshad","year":"2026","journal-title":"Biologia"},{"key":"10.1016\/j.mimet.2026.107642_bb0045","first-page":"1027","article-title":"Determinants of firms\u2019 sustainable development goals alignments: empirical insights from the Middle East green initiative","volume":"14","author":"Bagadeem","year":"2025","journal-title":"Int. J. Agric. Biosci."},{"key":"10.1016\/j.mimet.2026.107642_bb0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2022.133723","article-title":"Polyhydroxyalkanoates, the bioplastics of microbial origin: properties, biochemical synthesis, and their applications","volume":"294","author":"Behera","year":"2022","journal-title":"Chemosphere"},{"key":"10.1016\/j.mimet.2026.107642_bb0055","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/752821","article-title":"Isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper, and cardboard industry wastes","volume":"2013","author":"Bhuwal","year":"2013","journal-title":"Int. J. Biomater."},{"key":"10.1016\/j.mimet.2026.107642_bb0060","doi-asserted-by":"crossref","first-page":"6437","DOI":"10.1007\/s00253-018-9055-9","article-title":"Carbon flux to growth or polyhydroxyalkanoate synthesis under microaerophilic conditions is affected by fatty acid chain-length in Pseudomonas putida LS46","volume":"102","author":"Blunt","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2026.107642_bb0065","doi-asserted-by":"crossref","DOI":"10.3389\/feart.2024.1349865","article-title":"Micro- and nanoplastics in freshwater ecosystems\u2014interaction with and impact upon bacterivorous ciliates","volume":"12","author":"Bulannga","year":"2024","journal-title":"Front. Earth Sci."},{"key":"10.1016\/j.mimet.2026.107642_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.126906","article-title":"Rapid quantification of intracellular polyhydroxyalkanoates via fluorescence techniques: a critical review","volume":"350","author":"Cao","year":"2022","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0075","first-page":"61","article-title":"A solvent-free process to design low density polyhydroxyalkanoate","volume":"14","author":"Carvalho","year":"2023","journal-title":"Polym. Renew. Resour."},{"key":"10.1016\/j.mimet.2026.107642_bb0080","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s13213-010-0181-6","article-title":"Screening for polyhydroxyalkanoate (PHA)-producing bacterial strains and comparison of PHA production from various inexpensive carbon sources","volume":"61","author":"Chaudhry","year":"2011","journal-title":"Ann. Microbiol."},{"key":"10.1016\/j.mimet.2026.107642_bb0085","series-title":"Plastics from Bacteria. Microbiol. Monogr","first-page":"17","article-title":"Plastics completely synthesized by bacteria: polyhydroxyalkanoates","volume":"14","author":"Chen","year":"2010"},{"key":"10.1016\/j.mimet.2026.107642_bb0090","article-title":"Extrusion of PHA-containing bacterial biomass and the fate of endotoxins: a cost-reducing platform for applications in molding, coating and 3D printing","volume":"33","author":"Collet","year":"2022","journal-title":"Mater. Today Commun."},{"key":"10.1016\/j.mimet.2026.107642_bb0095","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.spc.2024.09.025","article-title":"Global projections of plastic use, end-of-life fate and potential changes in consumption, reduction, recycling and replacement with bioplastics to 2050","volume":"51","author":"Dokl","year":"2024","journal-title":"Sustainable Prod. Consumption"},{"key":"10.1016\/j.mimet.2026.107642_bb0100","article-title":"Integrated pretreatment and microbial matching for PHA production from lignocellulosic agro-forestry residues","volume":"11","author":"Dongna","year":"2025","journal-title":"Fermentation"},{"key":"10.1016\/j.mimet.2026.107642_bb0105","first-page":"1012","article-title":"Essential oil production and sage growth as influenced by treated wastewater irrigation and water stress","volume":"14","author":"Fattah","year":"2025","journal-title":"Int. J. Agric. Biosci."},{"key":"10.1016\/j.mimet.2026.107642_bb0110","first-page":"820","article-title":"Pollution, ecological and health risk assessment of heavy metals in urban soils of an industrial city in North-East Kazakhstan","volume":"13","author":"Faurat","year":"2024","journal-title":"Int. J. Agric. Biosci."},{"key":"10.1016\/j.mimet.2026.107642_bb0115","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.3390\/pr12081720","article-title":"Current status and challenges in the commercial production of polyhydroxyalkanoate-based bioplastic: a review","volume":"12","author":"Gautam","year":"2024","journal-title":"Processes"},{"key":"10.1016\/j.mimet.2026.107642_bb0120","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms13061350","article-title":"Diversity of mercury-tolerant microorganisms","volume":"13","author":"Golysheva","year":"2025","journal-title":"Microorganisms"},{"key":"10.1016\/j.mimet.2026.107642_bb0125","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.3390\/microorganisms12081668","article-title":"Advances in microbial biotechnology for sustainable alternatives to petroleum-based plastics: a comprehensive review of polyhydroxyalkanoate production","volume":"12","author":"Gonz\u00e1lez-Rojo","year":"2024","journal-title":"Microorganisms"},{"key":"10.1016\/j.mimet.2026.107642_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.rineng.2025.105345","article-title":"Polyhydroxyalkanoates (PHAs): key challenges in production and sustainable strategies for cost reduction within a circular economy framework","volume":"26","author":"Gundlapalli","year":"2025","journal-title":"Results Eng."},{"key":"10.1016\/j.mimet.2026.107642_bb0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2024.124821","article-title":"Drying strategies for maximizing polyhydroxybutyrate recovery from microalgae cultivated in a raceway pond: a comparative study","volume":"361","author":"Hassan","year":"2024","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.mimet.2026.107642_bb0140","first-page":"35","article-title":"Microplastics contamination in freshwater fish: a review","volume":"19","author":"Hayat","year":"2025","journal-title":"Agrobiol. Rec."},{"key":"10.1016\/j.mimet.2026.107642_bb0145","first-page":"271","article-title":"Evaluation of possible ameliorative role of robinetin to counteract polystyrene microplastics instigated renal toxicity in rats","volume":"44","author":"Ijaz","year":"2024","journal-title":"Pak. Vet. J."},{"key":"10.1016\/j.mimet.2026.107642_bb0150","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bej.2007.11.029","article-title":"Isolation and purification of bacterial poly(3-hydroxyalkanoates)","volume":"39","author":"Jacquel","year":"2008","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.mimet.2026.107642_bb0155","doi-asserted-by":"crossref","DOI":"10.3390\/polym15081937","article-title":"Exploiting polyhydroxyalkanoates for biomedical applications","volume":"15","author":"Kalia","year":"2023","journal-title":"Polymers"},{"key":"10.1016\/j.mimet.2026.107642_bb0160","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2025.120895","article-title":"Exploiting latent microbial potentials for producing polyhydroxyalkanoates: a holistic approach","volume":"269","author":"Kalia","year":"2025","journal-title":"Environ. Res."},{"key":"10.1016\/j.mimet.2026.107642_bb0165","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1016\/j.ijbiomac.2016.04.086","article-title":"Biosynthesis, purification and characterization of polyhydroxybutyrate from Botryococcus braunii K\u00fctz","volume":"89","author":"Kavitha","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107642_bb0170","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.ijbiomac.2021.01.108","article-title":"Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC\/MS)","volume":"174","author":"Khang","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107642_bb0175","first-page":"1","article-title":"Plastic pollution and global sustainability: a deep dive into environmental, health, and economic challenges","volume":"43","author":"Kiran","year":"2025","journal-title":"South Asian J. Zool."},{"key":"10.1016\/j.mimet.2026.107642_bb0180","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/BF02428979","article-title":"Staining method of poly(3-hydroxyalkanoic acids)-producing bacteria by Nile blue","volume":"8","author":"Kitamura","year":"1994","journal-title":"Biotechnol. Tech."},{"key":"10.1016\/j.mimet.2026.107642_bb0185","doi-asserted-by":"crossref","first-page":"74","DOI":"10.3390\/bioengineering9020074","article-title":"A new wave of industrialization of PHA biopolyesters","volume":"9","author":"Koller","year":"2022","journal-title":"Bioengineering"},{"key":"10.1016\/j.mimet.2026.107642_bb0190","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s10532-025-10164-y","article-title":"Biodegradability of polyhydroxyalkanoate (PHA) biopolyesters in nature: a review","volume":"36","author":"Koller","year":"2025","journal-title":"Biodegradation"},{"key":"10.1016\/j.mimet.2026.107642_bb0195","first-page":"560","article-title":"Role of tillage and bio-humus in the restoration of degraded pastures of Agropyrum pectiniforme in the steppe zone of Northeast Kazakhstan under changing climatic conditions","volume":"4","author":"Kukusheva","year":"2024","journal-title":"Int. J. Agric. Biosci."},{"key":"10.1016\/j.mimet.2026.107642_bb0200","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ijbiomac.2015.03.046","article-title":"Bioconversion of crude glycerol to polyhydroxyalkanoate by Bacillus thuringiensis under non-limiting nitrogen conditions","volume":"78","author":"Kumar","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107642_bb0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2024.143978","article-title":"Current methods for plastic waste recycling: challenges and opportunities","volume":"370","author":"Lee","year":"2025","journal-title":"Chemosphere"},{"key":"10.1016\/j.mimet.2026.107642_bb0210","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1007\/s00253-010-2611-6","article-title":"Identification of polyhydroxyalkanoates in Halococcus and other haloarchaeal species","volume":"87","author":"Legat","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mimet.2026.107642_bb0215","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.3390\/w14091419","article-title":"Copper bioremediation ability of ciliate Paramecium multimicronucleatum isolated from industrial wastewater","volume":"14","author":"Liaqat","year":"2022","journal-title":"Water"},{"key":"10.1016\/j.mimet.2026.107642_bb0220","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s00248-007-9329-8","article-title":"Screening and isolation of PHB-producing bacteria in a polluted marine microbial mat","volume":"56","author":"L\u00f3pez-Cort\u00e9s","year":"2008","journal-title":"Microb. Ecol."},{"key":"10.1016\/j.mimet.2026.107642_bb0225","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1126\/science.abg5433","article-title":"The global threat from plastic pollution","volume":"373","author":"MacLeod","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.mimet.2026.107642_bb0230","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.biortech.2018.01.002","article-title":"Production, process optimization and molecular characterization of polyhydroxyalkanoate (PHA) by CO\u2082 sequestering Bacillus cereus SS105","volume":"254","author":"Maheshwari","year":"2018","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0235","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/s41396-021-01110-w","article-title":"Microbial storage and its implications for soil ecology","volume":"16","author":"Mason-Jones","year":"2022","journal-title":"ISME J."},{"key":"10.1016\/j.mimet.2026.107642_bb0240","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1007\/s10924-012-0457-y","article-title":"Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Bacillus cereus S10","volume":"20","author":"Masood","year":"2012","journal-title":"J. Polym. Environ."},{"key":"10.1016\/j.mimet.2026.107642_bb0245","doi-asserted-by":"crossref","first-page":"5519","DOI":"10.1039\/D0GC01647K","article-title":"Review of recent advances in the biodegradability of polyhydroxyalkanoate (PHA) bioplastics and their composites","volume":"22","author":"Meereboer","year":"2020","journal-title":"Green Chem."},{"key":"10.1016\/j.mimet.2026.107642_bb0250","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.ecohyd.2023.04.004","article-title":"Paramecium jenningsi effectively grazes on toxic Raphidiopsis raciborskii and degrades cylindrospermopsin: implications for control of harmful cyanobacterial blooms","volume":"23","author":"Mohamed","year":"2023","journal-title":"Ecohydrol. Hydrobiol."},{"key":"10.1016\/j.mimet.2026.107642_bb0255","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2020.124439","article-title":"Polyhydroxyalkanoate synthesis and characterization: a proteogenomic and process optimization study for biovalorization of industrial lignin","volume":"320","author":"Morya","year":"2021","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0260","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2025.142850","article-title":"Advances and challenges in polyhydroxyalkanoates (PHA) production using Halomonas species: a review","volume":"309","author":"Muigano","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107642_bb0265","doi-asserted-by":"crossref","first-page":"855","DOI":"10.3390\/bioengineering10070855","article-title":"Microbial polyhydroxyalkanoate (PHA) biopolymers\u2014intrinsically natural","volume":"10","author":"Mukherjee","year":"2023","journal-title":"Bioengineering"},{"key":"10.1016\/j.mimet.2026.107642_bb0270","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1042\/EBC20200142","article-title":"Microbial cell factories for the production of polyhydroxyalkanoates","volume":"65","author":"Nagarajan","year":"2021","journal-title":"Essays Biochem."},{"key":"10.1016\/j.mimet.2026.107642_bb0275","first-page":"8","article-title":"Characterization of polyhydroxyalkanoates produced by Bacillus subtilis isolated from soil samples","volume":"1","author":"Nair","year":"2014","journal-title":"Malaya J. Biosci."},{"key":"10.1016\/j.mimet.2026.107642_bb0280","doi-asserted-by":"crossref","first-page":"97","DOI":"10.17509\/ijost.v4i1.15806","article-title":"How to read and interpret FTIR spectroscope of organic material","volume":"4","author":"Nandiyanto","year":"2019","journal-title":"Indones. J. Sci. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0285","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.3390\/polym16152227","article-title":"Valorization of algal biomass to produce microbial polyhydroxyalkanoates: recent updates, challenges, and perspectives","volume":"16","author":"Narayanasamy","year":"2024","journal-title":"Polymers"},{"key":"10.1016\/j.mimet.2026.107642_bb0290","doi-asserted-by":"crossref","DOI":"10.3390\/su162411124","article-title":"Mechanisms of heavy metal tolerance in bacteria: a review","volume":"16","author":"Nnaji","year":"2024","journal-title":"Sustainability"},{"key":"10.1016\/j.mimet.2026.107642_bb0295","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/j.ijbiomac.2022.03.102","article-title":"The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions","volume":"206","author":"Novackova","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107642_bb0300","author":"OECD"},{"key":"10.1016\/j.mimet.2026.107642_bb0305","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.biortech.2014.11.029","article-title":"Integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures","volume":"176","author":"Patel","year":"2015","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0310","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.3390\/polym16182570","article-title":"Polyhydroxyalkanoate production by methanotrophs: recent updates and perspectives","volume":"16","author":"Patel","year":"2024","journal-title":"Polymers"},{"key":"10.1016\/j.mimet.2026.107642_bb0315","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1038\/s41598-024-52098-0","article-title":"Production, optimization, scale-up and characterization of polyhydroxyalkanoate copolymers utilizing dairy processing waste","volume":"14","author":"Patil","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mimet.2026.107642_bb0320","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2019.107283","article-title":"A sustainable approach for the downstream processing of bacterial polyhydroxyalkanoates: state-of-the-art and latest developments","volume":"150","author":"P\u00e9rez-Rivero","year":"2019","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.mimet.2026.107642_bb0325","first-page":"14","article-title":"A review on climate change and occupational health: adapting workplaces to extreme weather events","volume":"1","author":"Qamar","year":"2025","journal-title":"Med. Plant Lett."},{"key":"10.1016\/j.mimet.2026.107642_bb0330","doi-asserted-by":"crossref","first-page":"7753","DOI":"10.1007\/s13399-022-02817-2","article-title":"Biodegradation and decolorization of reactive red 2 azo dye by Paramecium jenningsi and Paramecium multimicronucleatum in industrial wastewater","volume":"14","author":"Ramzan","year":"2022","journal-title":"Biomass Convers. Biorefinery"},{"key":"10.1016\/j.mimet.2026.107642_bb0335","doi-asserted-by":"crossref","first-page":"34","DOI":"10.3390\/bioengineering7020034","article-title":"Preparation and characterization of films based on a natural P(3HB)\/mcl-PHA blend obtained through the co-culture of Cupriavidus necator and Pseudomonas citronellolis in apple pulp waste","volume":"7","author":"Rebocho","year":"2020","journal-title":"Bioengineering"},{"key":"10.1016\/j.mimet.2026.107642_bb0340","article-title":"Polyhydroxyalkanoate production by Paramecium caudatum isolated from industrial wastewater: a microeukaryotic host for bioplastic accumulation","volume":"47","author":"Shabbir","year":"2025","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.mimet.2026.107642_bb0345","first-page":"79","article-title":"Nanoparticles: concept, types and applications in engineering technology (water treatment, energy production and biomedical technology)","volume":"4","author":"Shahzaib","year":"2025","journal-title":"Sci. Soc. Insights"},{"key":"10.1016\/j.mimet.2026.107642_bb0350","series-title":"Sustainable Biotechnology\u2014Enzymatic Resources of Renewable Energy","first-page":"355","article-title":"Microbially originated polyhydroxyalkanoate (PHA) biopolymers: an insight into the molecular mechanism and biogenesis of PHA granules","author":"Singh","year":"2018"},{"key":"10.1016\/j.mimet.2026.107642_bb0355","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2020.123536","article-title":"Critical overview of biomass feedstocks as sustainable substrates for the production of polyhydroxybutyrate (PHB)","volume":"311","author":"Sirohi","year":"2020","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0360","article-title":"Valorization of Musa paradisiaca peel waste for bioplastic production: a study on microbial degradation dynamics","author":"Talha","year":"2025","journal-title":"Waste Biomass Valor."},{"key":"10.1016\/j.mimet.2026.107642_bb0365","doi-asserted-by":"crossref","first-page":"706","DOI":"10.3390\/polym6030706","article-title":"Start a research on biopolymer polyhydroxyalkanoate (PHA): a review","volume":"6","author":"Tan","year":"2014","journal-title":"Polymers"},{"key":"10.1016\/j.mimet.2026.107642_bb0370","doi-asserted-by":"crossref","first-page":"937","DOI":"10.3390\/microorganisms11040937","article-title":"A review for the special issue on Paramecium as a modern model organism","volume":"11","author":"Van Houten","year":"2023","journal-title":"Microorganisms"},{"key":"10.1016\/j.mimet.2026.107642_bb0375","doi-asserted-by":"crossref","first-page":"7043","DOI":"10.1021\/acssuschemeng.5c00454","article-title":"Photodriven carbon nitride nanosheet\u2013bacterium biohybrid with an encapsulation structure for CO\u2082 to C4 bioplastic conversion","volume":"13","author":"Wang","year":"2025","journal-title":"ACS Sustain. Chem. Eng."},{"key":"10.1016\/j.mimet.2026.107642_bb0380","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/s12257-007-0138-1","article-title":"Enhancement of poly-3-hydroxybutyrate (PHB) productivity by the two-stage supplementation of carbon sources and continuous feeding of NH\u2084Cl","volume":"13","author":"Yeo","year":"2008","journal-title":"Biotechnol. Bioprocess Eng."},{"key":"10.1016\/j.mimet.2026.107642_bb0385","doi-asserted-by":"crossref","DOI":"10.1088\/2053-1591\/acc159","article-title":"Bioremediation of heavy metals using a novel species of ciliate Paramecium multimicronucleatum isolated from industrial wastewater","volume":"10","author":"Zahra","year":"2023","journal-title":"Mater. Res. Express"},{"key":"10.1016\/j.mimet.2026.107642_bb0390","doi-asserted-by":"crossref","DOI":"10.1016\/j.jprot.2024.105178","article-title":"Insights into the differential proteome landscape of a newly isolated Paramecium multimicronucleatum in response to cadmium stress","volume":"300","author":"Zahra","year":"2024","journal-title":"J. Proteome"},{"key":"10.1016\/j.mimet.2026.107642_bb0395","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1007\/s13762-024-05681-x","article-title":"Biosynthesis of polyhydroxyalkanoates employing a newly isolated Paramecium multimicronucleatum","volume":"22","author":"Zahra","year":"2025","journal-title":"Int. J. Environ. Sci. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0400","first-page":"1867","article-title":"Achieving high-rate hydrogen recovery from wastewater using customizable alginate polymer gel matrices encapsulating biomass","volume":"4","author":"Zhu","year":"2018","journal-title":"Environ. Sci.: Water Res. Technol."},{"key":"10.1016\/j.mimet.2026.107642_bb0405","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1039\/D3SU00126A","article-title":"A review on polyhydroxyalkanoate (PHA) production through the use of lignocellulosic biomass","volume":"1","author":"Zytner","year":"2023","journal-title":"RSC Sustain."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770122600254X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016770122600254X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:26:56Z","timestamp":1789046816000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016770122600254X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":81,"alternative-id":["S016770122600254X"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107642","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Solvent extraction and analytical characterization of polyhydroxyalkanoate inclusions produced by the microeukaryote Paramecium jenningsi","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107642","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107642"}}