{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:10:49Z","timestamp":1789049449297,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.mimet.2026.107675","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:23:37Z","timestamp":1787034217000},"page":"107675","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Benchmarking biofilm assays, surface models, and detachment methods in Pseudomonas aeruginosa for reproducible downstream molecular analysis"],"prefix":"10.1016","volume":"249","author":[{"given":"Mustafa","family":"Vohra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narayan","family":"Kamath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitin Pal","family":"Kalia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarika","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mimet.2026.107675_bb0005","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ijfoodmicro.2015.07.022","article-title":"Impact of growth temperature and surface type on the resistance of Pseudomonas aeruginosa and Staphylococcus aureus biofilms to disinfectants","volume":"214","author":"Abdallah","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.mimet.2026.107675_bb0010","doi-asserted-by":"crossref","first-page":"13779","DOI":"10.1038\/s41598-021-93115-w","article-title":"Interlaboratory study for the evaluation of three microtiter plate-based biofilm quantification methods","volume":"11","author":"Allkja","year":"2021","journal-title":"Sci. Report. UK"},{"key":"10.1016\/j.mimet.2026.107675_bb0015","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3109\/17453670902947457","article-title":"Sonication is superior to scraping for retrieval of bacteria in biofilm on titanium and steel surfaces in vitro","volume":"80","author":"Bjerkan","year":"2009","journal-title":"Acta Orthop."},{"key":"10.1016\/j.mimet.2026.107675_bb0020","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/s41522-018-0062-5","article-title":"Towards standardized mechanical characterization of microbial biofilms: analysis and critical review","volume":"4","author":"Boudarel","year":"2018","journal-title":"NPJ Biofilms Microbiomes"},{"key":"10.1016\/j.mimet.2026.107675_bb0025","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.3389\/fmicb.2017.02671","article-title":"Surface sensing for biofilm formation in Pseudomonas aeruginosa","volume":"8","author":"Chang","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2026.107675_bb0030","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.03518-22","article-title":"Material substrate physical properties control Pseudomonas aeruginosa biofilm architecture","author":"Cont","year":"2023","journal-title":"Mbio"},{"key":"10.1016\/j.mimet.2026.107675_bb0035","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/microorganisms7030073","article-title":"Biofilm specific activity: a measure to quantify microbial biofilm","volume":"7","author":"Corte","year":"2019","journal-title":"Microorg"},{"key":"10.1016\/j.mimet.2026.107675_bb0040","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.diagmicrobio.2012.11.019","article-title":"Comparison of three methods to study biofilm formation by clinical strains of Escherichia coli","volume":"75","author":"Cr\u00e9met","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2026.107675_bb0045","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00749-15","article-title":"The Pseudomonas aeruginosa transcriptional landscape is shaped by environmental heterogeneity and genetic variation","volume":"6","author":"D\u00f6tsch","year":"2015","journal-title":"mBio"},{"key":"10.1016\/j.mimet.2026.107675_bb0050","doi-asserted-by":"crossref","DOI":"10.3791\/63069","article-title":"Generation of greater bacterial biofilm biomass using PCR-plate deep well microplate devices","author":"Doucet","year":"2022","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.mimet.2026.107675_bb0055","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.mimet.2013.12.011","article-title":"Optimization of resazurin-based viability staining for quantification of microbial biofilms","volume":"98","author":"Driessche","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2026.107675_bb0060","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.81112","article-title":"Substrate stiffness impacts early biofilm formation by modulating Pseudomonas aeruginosa twitching motility","volume":"12","author":"Gomez","year":"2023","journal-title":"Elife"},{"key":"10.1016\/j.mimet.2026.107675_bb0065","first-page":"29","article-title":"Critical assessment of methods to quantify biofilm growth and evaluate antibiofilm activity of host defence peptides","volume":"8","author":"Haney","year":"2018","journal-title":"Biomol"},{"key":"10.1016\/j.mimet.2026.107675_bb0070","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s12866-024-03249-w","article-title":"In vitro investigation of relationship between quorum-sensing system genes, biofilm forming ability, and drug resistance in clinical isolates of Pseudomonas aeruginosa","volume":"24","author":"Hemmati","year":"2024","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.mimet.2026.107675_bb0075","doi-asserted-by":"crossref","DOI":"10.1128\/jb.00086-22","article-title":"Pseudomonas aeruginosa initiates a rapid and specific transcriptional response during surface attachment","volume":"204","author":"Jones","year":"2022","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mimet.2026.107675_bb0080","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.5740\/jaoacint.15-066","article-title":"Data quality in biofilm high-throughput routine analysis: intralaboratory protocol adaptation and experiment reproducibility","volume":"98","author":"Jorge","year":"2015","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.mimet.2026.107675_bb0085","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.diagmicrobio.2012.11.014","article-title":"Modification of the Congo red agar method to detect biofilm production by Staphylococcus epidermidis","volume":"75","author":"Kaiser","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2026.107675_bb0090","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2119434119","article-title":"Pseudomonas aeruginosa distinguishes surfaces by stiffness using retraction of type IV pili","volume":"119","author":"Koch","year":"2022","journal-title":"Proc. Nat. Acad. Sci."},{"key":"10.1016\/j.mimet.2026.107675_bb0095","article-title":"Interspecies and intraspecies \u2018talk\u2019 shape the bacterial biofilms","volume":"9","author":"Lou","year":"2025","journal-title":"Food Qual. Saf."},{"key":"10.1016\/j.mimet.2026.107675_bb0100","doi-asserted-by":"crossref","DOI":"10.1002\/mbo3.455","article-title":"Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of Enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model","volume":"6","author":"Mohmmed","year":"2017","journal-title":"MicrobiologyOpen"},{"key":"10.1016\/j.mimet.2026.107675_bb0105","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s11306-023-02080-0","article-title":"Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics","volume":"20","author":"Mosley","year":"2024","journal-title":"Metabolomics"},{"key":"10.1016\/j.mimet.2026.107675_bb0110","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1038\/nprot.2010.110","article-title":"A 96-well-plate\u2013based optical method for the quantitative and qualitative evaluation of Pseudomonas aeruginosa biofilm formation and its application to susceptibility testing","volume":"5","author":"M\u00fcsken","year":"2010","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mimet.2026.107675_bb0115","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/pj.2012.130","article-title":"Characteristics of bacterial biofilm associated with implant material in clinical practice","volume":"45","author":"Nandakumar","year":"2013","journal-title":"Polym. J."},{"key":"10.1016\/j.mimet.2026.107675_bb0120","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.892181","article-title":"Comparative study of different sampling methods of biofilm formed on stainless-steel surfaces in a CDC biofilm reactor","volume":"13","author":"Niboucha","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mimet.2026.107675_bb0125","doi-asserted-by":"crossref","DOI":"10.3791\/2437","article-title":"Microtiter dish biofilm formation assay","author":"O\u2019Toole","year":"2011","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.mimet.2026.107675_bb0130","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.mimet.2007.11.010","article-title":"Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates","volume":"72","author":"Peeters","year":"2008","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.mimet.2026.107675_bb0135","article-title":"Transcriptomic analysis of biofilm formation by Bacillus cereus under different carbon source conditions","volume":"8","author":"Qi","year":"2023","journal-title":"Food Qual. Saf."},{"key":"10.1016\/j.mimet.2026.107675_bb0140","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1186\/s13018-024-05309-3","article-title":"Biofilm and the effect of sonication in a chronic Staphylococcus epidermidis orthopedic in vivo implant infection model","volume":"19","author":"Sandbakken","year":"2024","journal-title":"J. Orthop. Surg. Res."},{"key":"10.1016\/j.mimet.2026.107675_bb0145","doi-asserted-by":"crossref","first-page":"295","DOI":"10.2144\/000114238","article-title":"Assessing and reducing sources of gene expression variability in Staphylococcus epidermidis biofilms","volume":"57","author":"Sousa","year":"2014","journal-title":"BioTechniques"},{"key":"10.1016\/j.mimet.2026.107675_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.128563","article-title":"A comprehensive review of genomics, transcriptomics, proteomics, and metabolomic insights into the differentiation of Pseudomonas aeruginosa from the planktonic to biofilm state: a multi-omics approach","volume":"257","author":"Vohra","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.mimet.2026.107675_bb0155","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3103\/S0891416825700077","article-title":"Complex interplay between biofilm formation, antibiotic resistance, and virulence in Pseudomonas aeruginosa: a phenotypic and genotypic study","volume":"40","author":"Vohra","year":"2025","journal-title":"Mol. Genet. Microbiol. Virol."},{"key":"10.1016\/j.mimet.2026.107675_bb0160","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.3390\/molecules26092723","article-title":"Potential of carvacrol and thymol in reducing biofilm formation on technical surfaces","volume":"26","author":"Walczak","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.mimet.2026.107675_bb0165","article-title":"Anti-virulence potential of carvone against Serratia marcescens","volume":"1","author":"Zhou","year":"2024","journal-title":"Food Med. Homol."},{"key":"10.1016\/j.mimet.2026.107675_bb0170","article-title":"Quorum sensing inhibitor: an effective strategy to attenuate the virulence and drug resistance of Pseudomonas aeruginosa","volume":"2","author":"Zhou","year":"2025","journal-title":"Food Med. Homol."}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226002873?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226002873?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:25:47Z","timestamp":1789046747000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226002873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":34,"alternative-id":["S0167701226002873"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107675","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Benchmarking biofilm assays, surface models, and detachment methods in Pseudomonas aeruginosa for reproducible downstream molecular analysis","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107675","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107675"}}