{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T14:36:59Z","timestamp":1793111819444,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiological Methods"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.mimet.2026.107694","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:01:33Z","timestamp":1788537693000},"page":"107694","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Optimised lysozyme-assisted TRIzol\u00ae extraction yields high-integrity RNA from the antibiotic-producing bacterium Amycolatopsis lurida"],"prefix":"10.1016","volume":"250","author":[{"given":"Lucy","family":"Butler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Bojko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Safwan","family":"Akram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.mimet.2026.107694_bb0005","doi-asserted-by":"crossref","DOI":"10.1016\/S1074-5521(98)90141-7","article-title":"Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699","volume":"5","author":"August","year":"1998","journal-title":"Chem. Biol."},{"issue":"8","key":"10.1016\/j.mimet.2026.107694_bb0010","doi-asserted-by":"crossref","DOI":"10.1016\/0168-9525(96)81467-3","article-title":"Short protocols in molecular biology (3rd edn)","volume":"12","author":"Baatout","year":"1996","journal-title":"Trends Genet."},{"issue":"5","key":"10.1016\/j.mimet.2026.107694_bb0015","article-title":"An efficient and high-yielding method for isolation of RNA from mycobacteria","volume":"17","author":"Bashyam","year":"1994","journal-title":"BioTechniques"},{"issue":"3","key":"10.1016\/j.mimet.2026.107694_bb0020","article-title":"Two different mechanisms for urea action at the LAC and TNA operons in Escherichia coli","volume":"220","author":"Blazy","year":"1990","journal-title":"Mol. Genet. Genet."},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0025","doi-asserted-by":"crossref","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mimet.2026.107694_bb0030","doi-asserted-by":"crossref","DOI":"10.3354\/dao03762","article-title":"Comparison of five commercial kits for isolation of total RNA in samples of WSSV-infected shrimp","volume":"156","author":"Encinas-Garc\u00eda","year":"2023","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.mimet.2026.107694_bb0035","doi-asserted-by":"crossref","DOI":"10.1186\/1741-7007-12-42","article-title":"RNA-seq: impact of RNA degradation on transcript quantification","volume":"12","author":"Gallego Romero","year":"2014","journal-title":"BMC Biol."},{"key":"10.1016\/j.mimet.2026.107694_bb0040","series-title":"A modication of cell-lysis for high-quality intact RNA of Bacillus subtilis extracted from the culture under different physiological stages","author":"Jaiaue","year":"2021"},{"key":"10.1016\/j.mimet.2026.107694_bb0045","series-title":"Practical Streptomyces Genetics","author":"Kieser","year":"2000"},{"issue":"5","key":"10.1016\/j.mimet.2026.107694_bb0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0097876","article-title":"Common contaminants in next-generation sequencing that hinder discovery of low-abundance microbes","volume":"9","author":"Laurence","year":"2014","journal-title":"PLoS One"},{"issue":"3","key":"10.1016\/j.mimet.2026.107694_bb0055","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2012.01.011","article-title":"Comparison of different bead-beating RNA extraction strategies: an optimized method for filamentous fungi","volume":"88","author":"Leite","year":"2012","journal-title":"J. Microbiol. Methods"},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0060","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-021-01521-6","article-title":"Comparative genomics and transcriptomics analyses provide insights into the high yield and regulatory mechanism of Norvancomycin biosynthesis in Amycolatopsis orientalis NCPC 2-48","volume":"20","author":"Li","year":"2021","journal-title":"Microb. Cell Factories"},{"issue":"19","key":"10.1016\/j.mimet.2026.107694_bb0065","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01066-21","article-title":"Enhancing Ristomycin a production by overexpression of ParBLike StrR family regulators controlling the biosynthesis genes","volume":"87","author":"Liu","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/0003-2697(87)90086-8","article-title":"Improved method for the isolation of RNA from plant tissues","volume":"163","author":"Logemann","year":"1987","journal-title":"Anal. Biochem."},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0075","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1046\/j.1472-765X.1997.00179.x","article-title":"New method for RNA isolation from actinomycetes: application to the transcriptional analysis of the alcohol oxidoreductase gene thcE in Rhodococcus and Mycobacterium","volume":"25","author":"Nagy","year":"1997","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.mimet.2026.107694_bb0080","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2020.595552","article-title":"Multi-omics analysis of the effect of cAMP on Actinorhodin production in Streptomyces coelicolor","volume":"8","author":"Nitta","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"3","key":"10.1016\/j.mimet.2026.107694_bb0085","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0214609","article-title":"Elimination of bacterial DNA during RNA isolation from sputum: bashing bead vortexing is preferable over prolonged DNase treatment","volume":"14","author":"Paska","year":"2019","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0090","doi-asserted-by":"crossref","DOI":"10.1111\/j.1472-765X.1995.tb00995.x","article-title":"A rapid protocol for isolation of RNA from mycobacteria","volume":"21","author":"Rajagopalan","year":"1995","journal-title":"Lett. Appl. Microbiol."},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0095","doi-asserted-by":"crossref","DOI":"10.1002\/0471142727.mb0404s21","article-title":"Preparation of bacterial RNA","volume":"21","author":"Reddy","year":"1993","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"10.1016\/j.mimet.2026.107694_bb0100","doi-asserted-by":"crossref","DOI":"10.1186\/1756-0500-3-211","article-title":"Comparison of commercial RNA extraction kits for preparation of DNA-free total RNA from Salmonella cells","volume":"3","author":"Rump","year":"2010","journal-title":"BMC Res. Notes"},{"issue":"4","key":"10.1016\/j.mimet.2026.107694_bb0105","doi-asserted-by":"crossref","DOI":"10.1099\/00222615-39-4-298","article-title":"Use of PCR-mediated amplification of Mycobacterium leprae DNA in different types of clinical samples for the diagnosis of leprosy","volume":"39","author":"Santos","year":"1993","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mimet.2026.107694_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.anaerobe.2013.09.007","article-title":"Efficiency of RNA extraction from selected bacteria in the context of biogas production and metatranscriptomics","volume":"29","author":"Stark","year":"2014","journal-title":"Anaerobe"},{"issue":"1","key":"10.1016\/j.mimet.2026.107694_bb0115","doi-asserted-by":"crossref","DOI":"10.1016\/j.mimet.2004.03.015","article-title":"Isolation of high quality RNA from Streptomyces","volume":"58","author":"Van Dessel","year":"2004","journal-title":"J. Microbiol. Methods"},{"issue":"9","key":"10.1016\/j.mimet.2026.107694_bb0120","doi-asserted-by":"crossref","DOI":"10.1007\/s10096-011-1191-4","article-title":"Comparison of three rapid and easy bacterial DNA extraction methods for use with quantitative real-time PCR","volume":"30","author":"Van Tongeren","year":"2011","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mimet.2026.107694_bb0125","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/21.20.4852","article-title":"Simple method of RNA isolation from human leucocytic cell lines","author":"Vauti","year":"1993","journal-title":"Nucleic Acids Res."},{"issue":"11","key":"10.1016\/j.mimet.2026.107694_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.105311","article-title":"Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples","volume":"25","author":"Verbeelen","year":"2022","journal-title":"iScience"},{"issue":"7","key":"10.1016\/j.mimet.2026.107694_bb0135","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/21.7.1682","article-title":"Rapid method of total RNA mini-preparation from eucaryotic cells","volume":"21","author":"Walther","year":"1993","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"10.1016\/j.mimet.2026.107694_bb0140","doi-asserted-by":"crossref","DOI":"10.1093\/biostatistics\/kxs001","article-title":"Modeling RNA degradation for RNA-Seq with applications","volume":"13","author":"Wan","year":"2012","journal-title":"Biostatistics"},{"issue":"2","key":"10.1016\/j.mimet.2026.107694_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/S0167-7012(02)00078-7","article-title":"Efficient isolation of total RNA from antibiotic-producing bacterium Amycolatopsis mediterranei","volume":"51","author":"Yao","year":"2002","journal-title":"J. Microbiol. Methods"}],"container-title":["Journal of Microbiological Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226003064?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0167701226003064?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T14:03:43Z","timestamp":1793109823000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0167701226003064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":29,"alternative-id":["S0167701226003064"],"URL":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107694","relation":{},"ISSN":["0167-7012"],"issn-type":[{"value":"0167-7012","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimised lysozyme-assisted TRIzol\u00ae extraction yields high-integrity RNA from the antibiotic-producing bacterium Amycolatopsis lurida","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiological Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mimet.2026.107694","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"107694"}}