{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T12:46:31Z","timestamp":1763988391942,"version":"build-2065373602"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,3,10]],"date-time":"2024-03-10T00:00:00Z","timestamp":1710028800000},"content-version":"am","delay-in-days":314,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Mitochondrion"],"published-print":{"date-parts":[[2023,5]]},"DOI":"10.1016\/j.mito.2023.02.010","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T12:50:51Z","timestamp":1677243051000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Development of a sensitive double TaqMan Probe-based qPCR Angle-Degree method to detect mutation frequencies"],"prefix":"10.1016","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1266-7948","authenticated-orcid":false,"given":"Kazuo","family":"Tomita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroko P.","family":"Indo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoaki","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitbanjong","family":"Tangpong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2842-0467","authenticated-orcid":false,"given":"Hideyuki J.","family":"Majima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"5806","key":"10.1016\/j.mito.2023.02.010_b0005","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/290457a0","article-title":"Sequence and organization of the human mitochondrial genome","volume":"290","author":"Anderson","year":"1981","journal-title":"Nature"},{"issue":"6","key":"10.1016\/j.mito.2023.02.010_b0010","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1373\/clinchem.2004.031153","article-title":"Detection and quantification of heteroplasmic mutant mitochondrial DNA by real-time amplification refractory mutation system quantitative PCR analysis: a single-step approach","volume":"50","author":"Bai","year":"2004","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mito.2023.02.010_b0015","doi-asserted-by":"crossref","first-page":"25186","DOI":"10.1038\/srep25186","article-title":"Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions","volume":"6","author":"Belmonte","year":"2016","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.mito.2023.02.010_b0020","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ymgme.2004.09.011","article-title":"Mutation screening of the mitochondrial genome using denaturing high-performance liquid chromatography","volume":"84","author":"Biggin","year":"2005","journal-title":"Mol. Genet. Metab."},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0025","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1101\/gr.396203","article-title":"Polymorphism ratio sequencing: a new approach for single nucleotide polymorphism discovery and genotyping","volume":"13","author":"Blazej","year":"2003","journal-title":"Genome Res."},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0030","first-page":"117","article-title":"Direct sequencing of amplified genomic fragments documents N-ras point mutations in myeloid leukemia","volume":"3","author":"Collins","year":"1988","journal-title":"Oncogene Res."},{"issue":"26","key":"10.1016\/j.mito.2023.02.010_b0035","doi-asserted-by":"crossref","first-page":"2656","DOI":"10.1056\/NEJMra022567","article-title":"Mitochondrial respiratory-chain diseases","volume":"348","author":"DiMauro","year":"2003","journal-title":"N. Engl. J. Med."},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0040","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3390\/molecules23020323","article-title":"Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations","volume":"23","author":"Duan","year":"2018","journal-title":"Molecules"},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0045","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/0006-291X(89)92452-2","article-title":"Detection of ras point mutations by polymerase chain reaction using mutation-specific, inosine-containing oligonucleotide primers","volume":"160","author":"Ehlen","year":"1989","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"3","key":"10.1016\/j.mito.2023.02.010_b0050","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1056\/NEJM199001183220307","article-title":"The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis","volume":"322","author":"Eisenstein","year":"1990","journal-title":"N. Engl. J. Med."},{"issue":"1\u20132","key":"10.1016\/j.mito.2023.02.010_b0055","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.mito.2006.11.026","article-title":"Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage","volume":"7","author":"Indo","year":"2007","journal-title":"Mitochondrion"},{"issue":"8","key":"10.1016\/j.mito.2023.02.010_b0060","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3109\/10715762.2012.698012","article-title":"Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line HLE","volume":"46","author":"Indo","year":"2012","journal-title":"Free Radic. Res."},{"issue":"1","key":"10.1016\/j.mito.2023.02.010_b0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3164\/jcbn.14-42","article-title":"A mitochondrial superoxide theory for oxidative stress diseases and aging","volume":"56","author":"Indo","year":"2015","journal-title":"J. Clin. Biochem. Nutr."},{"issue":"5","key":"10.1016\/j.mito.2023.02.010_b0070","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1136\/adc.82.5.407","article-title":"Heterogeneous presentation in A3243G mutation in the mitochondrial tRNA(Leu(UUR)) gene","volume":"82","author":"Koga","year":"2000","journal-title":"Arch. Did. Child."},{"issue":"12","key":"10.1016\/j.mito.2023.02.010_b0075","doi-asserted-by":"crossref","first-page":"2861","DOI":"10.1007\/s00415-018-9057-7","article-title":"Therapeutic regimen of L-arginine for MELAS: 9-year, prospective, multicenter, clinical research","volume":"265","author":"Koga","year":"2018","journal-title":"J. Neurol."},{"issue":"5","key":"10.1016\/j.mito.2023.02.010_b0080","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1586\/14737159.5.5.809","article-title":"Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic mutations","volume":"5","author":"Kraytsberg","year":"2005","journal-title":"Expert Rev. Mol. Diagn."},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0085","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.ajhg.2010.07.014","article-title":"Detecting heteroplasmy from high-throughput sequencing of complete human mitochondrial DNA genomes","volume":"87","author":"Li","year":"2010","journal-title":"Am. J. Hum. Genet."},{"issue":"8","key":"10.1016\/j.mito.2023.02.010_b0090","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1001\/jamaneurol.2016.0886","article-title":"Clinical Phenotype and Segregation of Mitochondrial 3243A>G Mutation in 2 Pairs of Monozygotic Twins","volume":"73","author":"Maeda","year":"2016","journal-title":"JAMA Neurol."},{"issue":"4","key":"10.1016\/j.mito.2023.02.010_b0095","first-page":"375","article-title":"An overview of mutation detection methods in genetic disorders Iran","volume":"23","author":"Mahdieh","year":"2013","journal-title":"J. Pediatr."},{"issue":"14","key":"10.1016\/j.mito.2023.02.010_b0100","doi-asserted-by":"crossref","first-page":"8217","DOI":"10.1074\/jbc.273.14.8217","article-title":"Prevention of mitochondrial injury by manganese superoxide dismutase reveals a primary mechanism for alkaline-induced cell death","volume":"273","author":"Majima","year":"1998","journal-title":"J. Biol. Chem."},{"issue":"14","key":"10.1016\/j.mito.2023.02.010_b0105","doi-asserted-by":"crossref","first-page":"4974","DOI":"10.1073\/pnas.84.14.4974","article-title":"Characterization of c-Ki-ras oncogene alleles by direct sequencing of enzymatically amplified DNA from carcinogen-induced tumors","volume":"84","author":"McMahon","year":"1987","journal-title":"PNAS"},{"key":"10.1016\/j.mito.2023.02.010_b0110","unstructured":"MITOMAP: http:\/\/www.mitomap.org."},{"key":"10.1016\/j.mito.2023.02.010_b0115","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0076-6879(87)55023-6","article-title":"Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction","volume":"155","author":"Mullis","year":"1987","journal-title":"Methods Enzymol."},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0120","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1432-1033.1978.tb12051.x","article-title":"Do mitochondria produce oxygen radicals in vivo?","volume":"82","author":"Nohl","year":"1978","journal-title":"Eur. J. Biochem."},{"issue":"11","key":"10.1016\/j.mito.2023.02.010_b0125","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1007\/s00125-002-0893-7","article-title":"Accumulation of somatic mutation in mitochondrial DNA extracted from peripheral blood cells in diabetic patients","volume":"45","author":"Nomiyama","year":"2002","journal-title":"Diabetologia"},{"issue":"8","key":"10.1016\/j.mito.2023.02.010_b0130","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1073\/pnas.86.8.2766","article-title":"Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms","volume":"86","author":"Orita","year":"1989","journal-title":"PNAS"},{"key":"10.1016\/j.mito.2023.02.010_b0135","doi-asserted-by":"crossref","first-page":"1286480","DOI":"10.1155\/2018\/1286480","article-title":"Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes","volume":"2018","author":"Rong","year":"2018","journal-title":"Biomed Res. Int."},{"issue":"12","key":"10.1016\/j.mito.2023.02.010_b0140","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"74","author":"Sanger","year":"1977","journal-title":"PNAS"},{"issue":"2","key":"10.1016\/j.mito.2023.02.010_b0145","doi-asserted-by":"crossref","first-page":"225","DOI":"10.2353\/jmoldx.2006.050067","article-title":"Rapid and sensitive real-time polymerase chain reaction method for detection and quantification of 3243A>G mitochondrial point mutation","volume":"8","author":"Singh","year":"2006","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.mito.2023.02.010_b0150","first-page":"2826","article-title":"Diabetes and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS): radiolabeled polymerase chain reaction is necessary for accurate detection of low percentages of mutation","volume":"82","author":"Smith","year":"1997","journal-title":"J. Clin. Endocrinol. Metab."},{"issue":"6","key":"10.1016\/j.mito.2023.02.010_b0155","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10815-007-9114-0","article-title":"The development of novel quantification assay for mitochondrial DNA heteroplasmy aimed at preimplantation genetic diagnosis of Leigh encephalopathy","volume":"24","author":"Tajima","year":"2007","journal-title":"J. Assit. Reprod. Gent."},{"issue":"12","key":"10.1016\/j.mito.2023.02.010_b0160","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1002\/(SICI)1096-9136(199712)14:12<1032::AID-DIA504>3.0.CO;2-Y","article-title":"Screening of patients with maternally transmitted diabetes for mitochondrial gene mutations in the tRNA[Leu(UUR)] region","volume":"14","author":"Tsukuda","year":"1997","journal-title":"Diabet. Med."},{"issue":"10","key":"10.1016\/j.mito.2023.02.010_b0165","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1093\/clinchem\/44.10.2088","article-title":"New sensitive method for the detection of the A3243G mutation of human mitochondrial deoxyribonucleic acid in diabetes mellitus patients by ligation-mediated polymerase chain reaction","volume":"44","author":"Urata","year":"1998","journal-title":"Clin. Chem."},{"issue":"11","key":"10.1016\/j.mito.2023.02.010_b0170","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1373\/clinchem.2004.033761","article-title":"High-sensitivity detection of the A3243G mutation of mitochondrial DNA by a combination of allele-specific PCR and peptide nucleic acid-directed PCR clamping","volume":"50","author":"Urata","year":"2004","journal-title":"Clin. Chem."},{"issue":"3","key":"10.1016\/j.mito.2023.02.010_b0175","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1089\/gte.2005.9.190","article-title":"Accurate detection and quantitation of heteroplasmic mitochondrial point mutations by pyrosequencing","volume":"9","author":"White","year":"2005","journal-title":"Genet. Test."}],"container-title":["Mitochondrion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567724923000193?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567724923000193?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T18:32:30Z","timestamp":1761589950000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567724923000193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":35,"alternative-id":["S1567724923000193"],"URL":"https:\/\/doi.org\/10.1016\/j.mito.2023.02.010","relation":{},"ISSN":["1567-7249"],"issn-type":[{"type":"print","value":"1567-7249"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a sensitive double TaqMan Probe-based qPCR Angle-Degree method to detect mutation frequencies","name":"articletitle","label":"Article Title"},{"value":"Mitochondrion","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mito.2023.02.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. and Mitochondria Research Society. All rights reserved.","name":"copyright","label":"Copyright"}]}}