{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T02:02:03Z","timestamp":1773972123893,"version":"3.50.1"},"reference-count":27,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010418","name":"Institute for Information and Communications Technology Promotion","doi-asserted-by":"publisher","award":["RS-2025-25463302"],"award-info":[{"award-number":["RS-2025-25463302"]}],"id":[{"id":"10.13039\/501100010418","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["RS-2025-17172968"],"award-info":[{"award-number":["RS-2025-17172968"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2024-00462540"],"award-info":[{"award-number":["RS-2024-00462540"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2024-00347015"],"award-info":[{"award-number":["RS-2024-00347015"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2023-00244107"],"award-info":[{"award-number":["RS-2023-00244107"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2025-15373099"],"award-info":[{"award-number":["RS-2025-15373099"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2021-NR059640"],"award-info":[{"award-number":["RS-2021-NR059640"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2025-25423510"],"award-info":[{"award-number":["RS-2025-25423510"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2024-00403897"],"award-info":[{"award-number":["RS-2024-00403897"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS-2024-00398456"],"award-info":[{"award-number":["RS-2024-00398456"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002551","name":"Seoul National University","doi-asserted-by":"publisher","award":["SNU-20230041"],"award-info":[{"award-number":["SNU-20230041"]}],"id":[{"id":"10.13039\/501100002551","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecules and Cells"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.mocell.2026.100311","type":"journal-article","created":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T06:31:50Z","timestamp":1767508310000},"page":"100311","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Viral transduction for T cell engineering in immunotherapy"],"prefix":"10.1016","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2481-8656","authenticated-orcid":false,"given":"Yikhyeon","family":"Seo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9341-5015","authenticated-orcid":false,"given":"Jimin","family":"Pak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0713-3672","authenticated-orcid":false,"given":"Jiyun","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2502-9147","authenticated-orcid":false,"given":"Joonbeom","family":"Bae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1635-4191","authenticated-orcid":false,"given":"Soo Seok","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mocell.2026.100311_bib1","doi-asserted-by":"crossref","first-page":"12537","DOI":"10.1128\/JVI.78.22.12537-12547.2004","article-title":"Murine T cells potently restrict human immunodeficiency virus infection","volume":"78","author":"Baumann","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.mocell.2026.100311_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2020.100152","article-title":"An improved protocol for the production of lentiviral vectors","volume":"1","author":"Brown","year":"2020","journal-title":"STAR Protoc."},{"key":"10.1016\/j.mocell.2026.100311_bib3","first-page":"Unit9.9","article-title":"Overview of the retrovirus transduction system","volume":"Chapter 9","author":"Cepko","year":"2001","journal-title":"Curr. Protoc.\u00a0Mol. Biol."},{"key":"10.1016\/j.mocell.2026.100311_bib4","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1016\/S0006-3495(04)74197-1","article-title":"Charged polymers modulate retrovirus transduction via membrane charge neutralization and virus aggregation","volume":"86","author":"Davis","year":"2004","journal-title":"Biophys. J."},{"key":"10.1016\/j.mocell.2026.100311_bib5","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/978-1-4939-3801-8_11","article-title":"Viral engineering of chimeric antigen receptor expression on murine and human T lymphocytes","volume":"1458","author":"Hammill","year":"2016","journal-title":"Methods Mol.\u00a0Biol."},{"key":"10.1016\/j.mocell.2026.100311_bib6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S1074-7613(00)80461-6","article-title":"The duration of antigenic stimulation determines the fate of naive and effector T cells","volume":"8","author":"Iezzi","year":"1998","journal-title":"Immunity"},{"key":"10.1016\/j.mocell.2026.100311_bib7","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1097\/CJI.0b013e3182187600","article-title":"Genetic engineering of murine CD8+ and CD4+ T cells for preclinical adoptive immunotherapy studies","volume":"34","author":"Kerkar","year":"2011","journal-title":"J. Immunother."},{"key":"10.1016\/j.mocell.2026.100311_bib8","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1002\/jgm.507","article-title":"Gene transfer with modified polyethylenimines","volume":"6","author":"Kichler","year":"2004","journal-title":"J.\u00a0Gene. Med."},{"key":"10.1016\/j.mocell.2026.100311_bib9","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1038\/nprot.2017.083","article-title":"Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function","volume":"12","author":"Kurachi","year":"2017","journal-title":"Nat.\u00a0Protoc."},{"key":"10.1016\/j.mocell.2026.100311_bib10","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.3390\/v13081528","article-title":"Lentiviral vectors for T cell engineering: clinical applications, bioprocessing and future perspectives","volume":"13","author":"Labbe","year":"2021","journal-title":"Viruses"},{"key":"10.1016\/j.mocell.2026.100311_bib11","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20192203","article-title":"Optimized gene engineering of murine CAR-T cells reveals the beneficial effects of IL-15 coexpression","volume":"218","author":"Lanitis","year":"2021","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.mocell.2026.100311_bib12","article-title":"Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction","volume":"6","author":"Lin","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.mocell.2026.100311_bib13","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1038\/sj.gt.3301206","article-title":"Plat-E: an efficient and stable system for transient packaging of retroviruses","volume":"7","author":"Morita","year":"2000","journal-title":"Gene.\u00a0Ther."},{"key":"10.1016\/j.mocell.2026.100311_bib14","doi-asserted-by":"crossref","first-page":"10074","DOI":"10.1128\/JVI.74.21.10074-10080.2000","article-title":"Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding","volume":"74","author":"O'doherty","year":"2000","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.mocell.2026.100311_bib15","first-page":"Unit9.11","article-title":"Transient transfection methods for preparation of high-titer retroviral supernatants","volume":"Chapter 9","author":"Pear","year":"2001","journal-title":"Curr.\u00a0Protoc. Mol.\u00a0Biol."},{"key":"10.1016\/j.mocell.2026.100311_bib16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s12860-021-00397-z","article-title":"Spinoculation and retronectin highly enhance the gene transduction efficiency of Mucin-1-specific chimeric antigen receptor (CAR) in human primary T cells","volume":"22","author":"Rajabzadeh","year":"2021","journal-title":"BMC Mol. Cell.\u00a0Biol."},{"key":"10.1016\/j.mocell.2026.100311_bib17","first-page":"Unit 16.21","article-title":"Overview of the HIV-1 lentiviral vector system","volume":"Chapter 16","author":"Ramezani","year":"2002","journal-title":"Curr. Protoc. Mol.\u00a0Biol."},{"key":"10.1016\/j.mocell.2026.100311_bib18","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1002\/j.1460-2075.1993.tb05858.x","article-title":"Integration of murine leukemia virus DNA depends on mitosis","volume":"12","author":"Roe","year":"1993","journal-title":"EMBO J."},{"key":"10.1016\/j.mocell.2026.100311_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2024.103529","article-title":"Protocol for production of tonic CAR T cells with dasatinib","volume":"6","author":"Rosselle","year":"2025","journal-title":"STAR Protoc."},{"key":"10.1016\/j.mocell.2026.100311_bib20","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/nature22395","article-title":"Therapeutic T cell engineering","volume":"545","author":"Sadelain","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.mocell.2026.100311_bib21","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1146\/annurev.bioeng.10.061807.160514","article-title":"Molecular engineering of viral gene delivery vehicles","volume":"10","author":"Schaffer","year":"2008","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"10.1016\/j.mocell.2026.100311_bib22","first-page":"305","article-title":"T cell anergy","volume":"21","author":"Schwartz","year":"2003","journal-title":"Annu.\u00a0Rev.\u00a0Immunol."},{"key":"10.1016\/j.mocell.2026.100311_bib23","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.1016\/j.cell.2018.10.024","article-title":"Genome-wide CRISPR screens in primary human T cells reveal key regulators of immune function","volume":"175","author":"Shifrut","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.mocell.2026.100311_bib24","article-title":"Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems","author":"Swift","year":"2001","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.mocell.2026.100311_bib25","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1021\/bp0500314","article-title":"Optimized retroviral transduction protocol which preserves the primitive subpopulation of human hematopoietic cells","volume":"21","author":"Tonks","year":"2005","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.mocell.2026.100311_bib26","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1038\/s41590-020-0725-2","article-title":"Impaired mitochondrial oxidative phosphorylation limits the self-renewal of T cells exposed to persistent antigen","volume":"21","author":"Vardhana","year":"2020","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.mocell.2026.100311_bib27","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-1-59745-409-4_8","article-title":"Lentiviral vector gene transfer into human T cells","volume":"506","author":"Verhoeyen","year":"2009","journal-title":"Methods Mol. Biol."}],"container-title":["Molecules and Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1016847826000026?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1016847826000026?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T01:00:50Z","timestamp":1773968450000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1016847826000026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["S1016847826000026"],"URL":"https:\/\/doi.org\/10.1016\/j.mocell.2026.100311","relation":{},"ISSN":["1016-8478"],"issn-type":[{"value":"1016-8478","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Viral transduction for T cell engineering in immunotherapy","name":"articletitle","label":"Article Title"},{"value":"Molecules and Cells","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mocell.2026.100311","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"simple-article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology.","name":"copyright","label":"Copyright"}],"article-number":"100311"}}