{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:29:14Z","timestamp":1777516154536,"version":"3.51.4"},"reference-count":47,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54 CA268069"],"award-info":[{"award-number":["U54 CA268069"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 GM101701"],"award-info":[{"award-number":["R01 GM101701"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["mp.uscap.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Modern Pathology"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.modpat.2025.100768","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T17:58:53Z","timestamp":1744135133000},"page":"100768","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Polychromatic Polarization Microscopy Differentiates Collagen Fiber Signatures in Benign Pancreatic Tissue and Pancreatic Ductal Adenocarcinoma"],"prefix":"10.1016","volume":"38","author":[{"given":"Mahsa","family":"Chitsaz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rania","family":"Rayes-Danan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omid","family":"Savari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Shribak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Eliceiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5827-7022","authenticated-orcid":false,"given":"Agnes","family":"Loeffler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s40170-020-00221-w","article-title":"Tumor-associated collagen signatures: pushing tumor boundaries","volume":"8","author":"Brett","year":"2020","journal-title":"Cancer Metab"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1741-7015-6-11","article-title":"Collagen density promotes mammary tumor initiation and progression","volume":"6","author":"Provenzano","year":"2008","journal-title":"BMC Med"},{"issue":"8","key":"10.1016\/j.modpat.2025.100768_bib3","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/nrgastro.2012.115","article-title":"The role of stroma in pancreatic cancer: diagnostic and therapeutic implications","volume":"9","author":"Erkan","year":"2012","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"4","key":"10.1016\/j.modpat.2025.100768_bib4","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1097\/PPO.0000000000000140","article-title":"Orchestrating the tumor microenvironment to improve survival for patients with pancreatic cancer: normalization, not destruction","volume":"21","author":"Whatcott","year":"2015","journal-title":"Cancer J"},{"issue":"11","key":"10.1016\/j.modpat.2025.100768_bib5","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1038\/modpathol.2015.97","article-title":"Periductal stromal collagen topology of pancreatic ductal adenocarcinoma differs from that of normal and chronic pancreatitis","volume":"28","author":"Drifka","year":"2015","journal-title":"Mod Pathol"},{"issue":"7","key":"10.1016\/j.modpat.2025.100768_bib6","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0234568","article-title":"Alignment of stroma fibers, microvessel density and immune cell populations determine overall survival in pancreatic cancer-An analysis of stromal morphology","volume":"15","author":"Bolm","year":"2020","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.modpat.2025.100768_bib7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3390\/bioengineering8020017","article-title":"Navigating the collagen jungle: the biomedical potential of fiber organization in cancer","volume":"8","author":"Ouellette","year":"2021","journal-title":"Bioengineering (Basel)"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib8","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1158\/1055-9965.EPI-20-0889","article-title":"Collagen organization in relation to ductal carcinoma in situ pathology and outcomes","volume":"30","author":"Sprague","year":"2021","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"issue":"40","key":"10.1016\/j.modpat.2025.100768_bib9","doi-asserted-by":"crossref","first-page":"11208","DOI":"10.1073\/pnas.1610347113","article-title":"Oriented collagen fibers direct tumor cell intravasation","volume":"113","author":"Han","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"10.1016\/j.modpat.2025.100768_bib10","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.ajpath.2010.11.076","article-title":"Aligned collagen is a prognostic signature for survival in human breast carcinoma","volume":"178","author":"Conklin","year":"2011","journal-title":"Am J Pathol"},{"issue":"2","key":"10.1016\/j.modpat.2025.100768_bib11","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1158\/1055-9965.EPI-17-0720","article-title":"Collagen alignment as a predictor of recurrence after ductal carcinoma in situ","volume":"27","author":"Conklin","year":"2018","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib12","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/1741-7015-4-38","article-title":"Collagen reorganization at the tumor-stromal interface facilitates local invasion","volume":"4","author":"Provenzano","year":"2006","journal-title":"BMC Med"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib13","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-31126-w","article-title":"Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma","volume":"8","author":"Tomko","year":"2018","journal-title":"Sci Rep"},{"issue":"3","key":"10.1016\/j.modpat.2025.100768_bib14","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.ajpath.2017.10.025","article-title":"The presence of cyclooxygenase 2, tumor-associated macrophages, and collagen alignment as prognostic markers for invasive breast carcinoma patients","volume":"188","author":"Esbona","year":"2018","journal-title":"Am J Pathol"},{"issue":"5933","key":"10.1016\/j.modpat.2025.100768_bib15","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1126\/science.1171362","article-title":"Inhibition of Hedgehog signaling enhances delivery of chemotherapy in a mouse model of pancreatic cancer","volume":"324","author":"Olive","year":"2009","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib16","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.cell.2014.08.007","article-title":"Vitamin D receptor-mediated stromal reprogramming suppresses pancreatitis and enhances pancreatic cancer therapy","volume":"159","author":"Sherman","year":"2014","journal-title":"Cell"},{"issue":"3","key":"10.1016\/j.modpat.2025.100768_bib17","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.ccr.2012.01.007","article-title":"Enzymatic targeting of the stroma ablates physical barriers to treatment of pancreatic ductal adenocarcinoma","volume":"21","author":"Provenzano","year":"2012","journal-title":"Cancer Cell"},{"issue":"8","key":"10.1016\/j.modpat.2025.100768_bib18","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1364\/BOE.2.002307","article-title":"Structural changes in mixed Col I\/Col V collagen gels probed by SHG microscopy: implications for probing stromal alterations in human breast cancer","volume":"2","author":"Ajeti","year":"2011","journal-title":"Biomed Opt Express"},{"issue":"3","key":"10.1016\/j.modpat.2025.100768_bib19","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.150330","article-title":"Stromal architecture directs early dissemination in pancreatic ductal adenocarcinoma","volume":"7","author":"Ray","year":"2022","journal-title":"JCI Insight"},{"key":"10.1016\/j.modpat.2025.100768_bib20","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ceb.2021.05.004","article-title":"Aligned forces: origins and mechanisms of cancer dissemination guided by extracellular matrix architecture","volume":"72","author":"Ray","year":"2021","journal-title":"Curr Opin Cell Biol"},{"issue":"3-1","key":"10.1016\/j.modpat.2025.100768_bib21","article-title":"Realizations of highly heterogeneous collagen networks via stochastic reconstruction for micromechanical analysis of tumor cell invasion","volume":"97","author":"Nan","year":"2018","journal-title":"Phys Rev E"},{"issue":"4","key":"10.1016\/j.modpat.2025.100768_bib22","doi-asserted-by":"crossref","first-page":"991","DOI":"10.3390\/jcm9040991","article-title":"Oncogenic linear collagen VI of invasive breast cancer is induced by CCL5","volume":"9","author":"Brett","year":"2020","journal-title":"J Clin Med"},{"issue":"5","key":"10.1016\/j.modpat.2025.100768_bib23","doi-asserted-by":"crossref","first-page":"e360","DOI":"10.1016\/j.clbc.2024.02.015","article-title":"Stromal type I collagen in breast cancer: correlation to prognostic biomarkers and prediction of chemotherapy response","volume":"24","author":"Jansson","year":"2024","journal-title":"Clin Breast Cancer"},{"issue":"11","key":"10.1016\/j.modpat.2025.100768_bib24","doi-asserted-by":"crossref","first-page":"2546","DOI":"10.1016\/j.bpj.2014.10.035","article-title":"3D Collagen alignment limits protrusions to enhance breast cancer cell persistence","volume":"107","author":"Riching","year":"2014","journal-title":"Biophys J"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib25","doi-asserted-by":"crossref","first-page":"28","DOI":"10.4103\/2153-3539.139707","article-title":"Automated quantification of aligned collagen for human breast carcinoma prognosis","volume":"5","author":"Bredfeldt","year":"2014","journal-title":"J Pathol Inform"},{"issue":"7","key":"10.1016\/j.modpat.2025.100768_bib26","doi-asserted-by":"crossref","first-page":"3229","DOI":"10.7150\/thno.55921","article-title":"Large-scale tumor-associated collagen signatures identify high-risk breast cancer patients","volume":"11","author":"Xi","year":"2021","journal-title":"Theranostics"},{"issue":"9","key":"10.1016\/j.modpat.2025.100768_bib27","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1136\/gutjnl-2015-309304","article-title":"Stromal biology and therapy in pancreatic cancer: a changing paradigm","volume":"64","author":"Neesse","year":"2015","journal-title":"Gut"},{"issue":"8","key":"10.1016\/j.modpat.2025.100768_bib28","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.3390\/cancers16081560","article-title":"A perspective review: analyzing collagen alterations in ovarian cancer by high-resolution optical microscopy","volume":"16","author":"Gant","year":"2024","journal-title":"Cancers"},{"issue":"6","key":"10.1016\/j.modpat.2025.100768_bib29","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.3390\/cancers12061390","article-title":"Role of collagen fiber morphology on ovarian cancer cell migration using image-based models of the extracellular matrix","volume":"12","author":"Alkmin","year":"2020","journal-title":"Cancers"},{"issue":"6","key":"10.1016\/j.modpat.2025.100768_bib30","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.3171\/2016.6.JNS152797","article-title":"Association of collagen architecture with glioblastoma patient survival","volume":"126","author":"Pointer","year":"2016","journal-title":"J Neurosurg"},{"issue":"3","key":"10.1016\/j.modpat.2025.100768_bib31","doi-asserted-by":"crossref","first-page":"2746","DOI":"10.3390\/ijms24032746","article-title":"Machine learning quantified tumor-stroma ratio is an independent prognosticator in muscle-invasive bladder cancer","volume":"24","author":"Zheng","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"1-2","key":"10.1016\/j.modpat.2025.100768_bib32","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/jbio.201200108","article-title":"Second harmonic generation microscopy as a powerful diagnostic imaging modality for human ovarian cancer","volume":"7","author":"Adur","year":"2014","journal-title":"J Biophotonics"},{"issue":"9","key":"10.1016\/j.modpat.2025.100768_bib33","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1364\/BOE.3.002021","article-title":"Quantifying collagen structure in breast biopsies using second-harmonic generation imaging","volume":"3","author":"Ambekar","year":"2012","journal-title":"Biomed Opt Express"},{"issue":"2","key":"10.1016\/j.modpat.2025.100768_bib34","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1365-2818.2012.03629.x","article-title":"Imaging and 3D morphological analysis of collagen fibrils","volume":"247","author":"Altendorf","year":"2012","journal-title":"J Microsc"},{"issue":"12","key":"10.1016\/j.modpat.2025.100768_bib35","doi-asserted-by":"crossref","first-page":"7075","DOI":"10.1073\/pnas.0832308100","article-title":"Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation","volume":"100","author":"Zipfel","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"10.1016\/j.modpat.2025.100768_bib36","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/BF01002772","article-title":"Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections","volume":"11","author":"Junqueira","year":"1979","journal-title":"Histochem J"},{"issue":"10","key":"10.1016\/j.modpat.2025.100768_bib37","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1369\/0022155414545787","article-title":"Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues","volume":"62","author":"Lattouf","year":"2014","journal-title":"J Histochem Cytochem"},{"issue":"10","key":"10.1016\/j.modpat.2025.100768_bib38","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1369\/00221554211046777","article-title":"Picrosirius red staining: revisiting its application to the qualitative and quantitative assessment of collagen type I and type III in tendon","volume":"69","author":"L\u00f3pez De Padilla","year":"2021","journal-title":"J Histochem Cytochem"},{"issue":"9","key":"10.1016\/j.modpat.2025.100768_bib39","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1369\/0022155416659249","article-title":"Comparison of picrosirius red staining with second harmonic generation imaging for the quantification of clinically relevant collagen fiber features in histopathology samples","volume":"64","author":"Drifka","year":"2016","journal-title":"J Histochem Cytochem"},{"issue":"8","key":"10.1016\/j.modpat.2025.100768_bib40","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1369\/0022155417718541","article-title":"Fluorescence of picrosirius red multiplexed with immunohistochemistry for the quantitative assessment of collagen in tissue sections","volume":"65","author":"Wegner","year":"2017","journal-title":"J Histochem Cytochem"},{"issue":"19","key":"10.1016\/j.modpat.2025.100768_bib41","doi-asserted-by":"crossref","first-page":"22561","DOI":"10.1364\/OE.22.022561","article-title":"Determination of collagen fiber orientation in histological slides using Mueller microscopy and validation by second harmonic generation imaging","volume":"22","author":"Bancelin","year":"2014","journal-title":"Opt Express"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib42","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/s42003-020-01151-5","article-title":"Non-disruptive collagen characterization in clinical histopathology using cross-modality image synthesis","volume":"3","author":"Keikhosravi","year":"2020","journal-title":"Commun Biol"},{"issue":"10","key":"10.1016\/j.modpat.2025.100768_bib43","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1016\/j.ajpath.2021.04.008","article-title":"Machine-learning\u2013based evaluation of intratumoral heterogeneity and tumor-stroma interface for clinical guidance","volume":"191","author":"Laurinavicius","year":"2021","journal-title":"Am J Pathol"},{"key":"10.1016\/j.modpat.2025.100768_bib44","doi-asserted-by":"crossref","DOI":"10.3389\/fonc.2021.707288","article-title":"CT radiomics and machine-learning models for predicting tumor-stroma ratio in patients with pancreatic ductal adenocarcinoma","volume":"11","author":"Meng","year":"2021","journal-title":"Front Oncol"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib45","doi-asserted-by":"crossref","DOI":"10.1038\/srep17340","article-title":"Polychromatic polarization microscope: bringing colors to a colorless world","volume":"5","author":"Shribak","year":"2015","journal-title":"Sci Rep"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib46","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1080\/02678292.2022.2142883","article-title":"Instantaneous mapping of liquid crystal orientation using a polychromatic polarizing microscope","volume":"50","author":"Rajabi","year":"2023","journal-title":"Liq Cryst"},{"issue":"1","key":"10.1016\/j.modpat.2025.100768_bib47","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98600-w","article-title":"Real-time polarization microscopy of fibrillar collagen in histopathology","volume":"11","author":"Keikhosravi","year":"2021","journal-title":"Sci Rep"}],"container-title":["Modern Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089339522500064X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089339522500064X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T13:05:52Z","timestamp":1755695152000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S089339522500064X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":47,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["S089339522500064X"],"URL":"https:\/\/doi.org\/10.1016\/j.modpat.2025.100768","relation":{},"ISSN":["0893-3952"],"issn-type":[{"value":"0893-3952","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Polychromatic Polarization Microscopy Differentiates Collagen Fiber Signatures in Benign Pancreatic Tissue and Pancreatic Ductal Adenocarcinoma","name":"articletitle","label":"Article Title"},{"value":"Modern Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.modpat.2025.100768","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 THE AUTHORS. Published by Elsevier Inc. on behalf of the United States & Canadian Academy of Pathology.","name":"copyright","label":"Copyright"}],"article-number":"100768"}}