{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T04:12:31Z","timestamp":1776831151564,"version":"3.51.2"},"reference-count":51,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n, Spain","doi-asserted-by":"crossref","award":["AGL2006-13936-C02 and AGL2010-22290-C03"],"award-info":[{"award-number":["AGL2006-13936-C02 and AGL2010-22290-C03"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"name":"European Fund for Regional Development (FEDER)"},{"name":"Xunta de Galicia, Spain","award":["INCITE09 PXIB 203 206 PR"],"award-info":[{"award-number":["INCITE09 PXIB 203 206 PR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular and Biochemical Parasitology"],"published-print":{"date-parts":[[2011,10]]},"DOI":"10.1016\/j.molbiopara.2011.06.003","type":"journal-article","created":{"date-parts":[[2011,6,28]],"date-time":"2011-06-28T17:55:06Z","timestamp":1309283706000},"page":"80-90","source":"Crossref","is-referenced-by-count":37,"title":["Molecular and immunological characterization of Fasciola antigens recognized by the MM3 monoclonal antibody"],"prefix":"10.1016","volume":"179","author":[{"given":"L.","family":"Mui\u00f1o","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.J.","family":"Perteguer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"G\u00e1rate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Mart\u00ednez-Sern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Beltr\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Romar\u00eds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mezo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gonz\u00e1lez-Warleta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.M.","family":"Ubeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.molbiopara.2011.06.003_bib0005","first-page":"340","article-title":"Epidemiology of human fascioliasis: a review and proposed new classification","volume":"77","author":"Mas-Coma","year":"1999","journal-title":"Bull World Health Organ"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0010","series-title":"Fasciolosis","first-page":"435","article-title":"Immunodiagnosis of human and animal fascioliasis","author":"Hillyer","year":"1999"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2011.03.009","article-title":"Association between anti-F. hepatica antibody levels in milk and production losses in dairy cows","author":"Mezo","year":"2011","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0020","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.3201\/eid1110.050614","article-title":"Emerging foodborne trematodiasis","volume":"11","author":"Keiser","year":"2005","journal-title":"Emerg Infect Dis"},{"issue":"Suppl.","key":"10.1016\/j.molbiopara.2011.06.003_bib0025","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1017\/S0031182006001806","article-title":"Vaccines against the zoonotic trematodes Schistosoma japonicum Fasciola hepatica and Fasciola gigantica","volume":"133","author":"McManus","year":"2006","journal-title":"Parasitology"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0030","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0065-308X(09)69002-3","article-title":"Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control","volume":"69","author":"Mas-Coma","year":"2009","journal-title":"Adv Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0035","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/0007-1935(91)90003-6","article-title":"The pharmacology of flukicide drugs","volume":"147","author":"McKellar","year":"1991","journal-title":"Br Vet J"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0040","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0304-4017(00)00267-3","article-title":"Resistance of Fasciola hepatica against triclabendazole in cattle and sheep in the Netherlands","volume":"91","author":"Moll","year":"2000","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0045","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1136\/vr.159.13.424","article-title":"Resistance of Fasciola hepatica to triclabendazole and albendazole in sheep in Spain","volume":"159","author":"\u00c1lvarez-S\u00e1nchez","year":"2006","journal-title":"Vet Rec"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0050","first-page":"R1","article-title":"Progress in assessment of morbidity due to Fasciola hepatica infections: a review of recent literature","volume":"87","author":"Chen","year":"1990","journal-title":"Trop Dis Bull"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0055","doi-asserted-by":"crossref","first-page":"156","DOI":"10.4269\/ajtmh.2009.81.156","article-title":"MM3-ELISA detection of Fasciola hepatica coproantigens in preserved human stool samples","volume":"8","author":"Ubeira","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0060","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1645\/GE-925R.1","article-title":"The use of MM3 monoclonal antibodies for the early immunodiagnosis of ovine fascioliasis","volume":"93","author":"Mezo","year":"2007","journal-title":"J Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0065","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.parint.2010.09.001","article-title":"Field evaluation of the MM3-SERO ELISA for detection of anti-Fasciola IgG antibodies in milk samples from individual cows and bulk milk tanks","volume":"59","author":"Mezo","year":"2010","journal-title":"Parasitol Int"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0070","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.vetpar.2009.10.007","article-title":"Kinetics of anti-Fasciola IgG antibodies in serum and milk from dairy cows during lactation, and in serum from calves after feeding colostrum from infected dams","volume":"168","author":"Mezo","year":"2010","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0075","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1128\/JCM.32.1.190-193.1994","article-title":"Sandwich enzyme-linked immunosorbent assay for detection of excretory secretory antigens in humans with fascioliasis","volume":"32","author":"Espino","year":"1994","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0080","doi-asserted-by":"crossref","first-page":"533","DOI":"10.2460\/ajvr.1998.59.5.533","article-title":"Evaluation of a diagnostic monoclonal antibody-based capture enzyme-linked immunosorbent assay for detection of a 26- to 28-kd Fasciola hepatica coproantigen in cattle","volume":"59","author":"Abdel-Rahman","year":"1998","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0085","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1645\/GE-192R","article-title":"An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3)","volume":"90","author":"Mezo","year":"2004","journal-title":"J Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0090","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1645\/GE-74RI.1","article-title":"Optimized serodiagnosis of sheep fascioliasis by Fast-D protein liquid chromatography fractionation of Fasciola hepatica excretory\u2013secretory antigens","volume":"89","author":"Mezo","year":"2003","journal-title":"J Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0095","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of the bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0100","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0166-6851(94)90130-9","article-title":"Cloning of a protease gene family of Fasciola hepatica by the polymerase chain reaction","volume":"64","author":"Heussler","year":"1994","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0105","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.1016\/S0264-410X(00)00095-5","article-title":"Successful DNA immunisation of rats against fasciolosis","volume":"18","author":"Kofta","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0110","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1038\/nprot.2007.131","article-title":"Locating proteins in the cell using TargetP SignalP, and related tools","volume":"2","author":"Emanuelsson","year":"2007","journal-title":"Nature Protoc"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0115","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1128\/JB.120.1.466-474.1974","article-title":"\u03b2-Galactosidase from termination and deletion mutant strains","volume":"120","author":"Villarejo","year":"1974","journal-title":"J Bacteriol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0120","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/BF00930905","article-title":"Anatomical location of phosphorylcholine and other antigens on encysted Trichinella using immunohistochemistry followed by Wheatley's trichrome stain","volume":"77","author":"Sanmart\u00edn","year":"1991","journal-title":"Parasitol Res"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0125","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0166-6851(95)02478-6","article-title":"Characterisation of a novel Kunitz-type molecule from the trematode Fasciola hepatica","volume":"74","author":"Bozas","year":"1995","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0130","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/S0020-7519(03)00171-1","article-title":"Fasciola hepatica cathepsin L-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines","volume":"33","author":"Dalton","year":"2003","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0135","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1074\/mcp.M700560-MCP200","article-title":"Proteomics and phylogenetic analysis of the cathepsin L protease family of the helminth pathogen Fasciola hepatica: expansion of a repertoire of virulence-associated factors","volume":"7","author":"Robinson","year":"2008","journal-title":"Mol Cell Proteomics"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0140","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.vetpar.2008.10.014","article-title":"MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica","volume":"159","author":"Valero","year":"2008","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0145","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetpar.2008.03.017","article-title":"Immunodiagnostic\/protective role of cathepsin L cysteine proteinases secreted by Fasciola species","volume":"154","author":"Dixit","year":"2008","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0150","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0732-8893(02)00431-5","article-title":"Diagnosis of human fasciolosis in the Gilan province of Northern Iran: application of cathepsin L-ELISA","volume":"44","author":"Rokni","year":"2002","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0155","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.diagmicrobio.2005.05.010","article-title":"Potent epitopes derived from Fasciola gigantica cathepsin L1 in peptide-based immunoassay for the serodiagnosis of human fascioliasis","volume":"53","author":"Intapan","year":"2005","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0160","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/S0020-7519(01)00175-8","article-title":"Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease","volume":"31","author":"Cornelissen","year":"2001","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0165","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetpar.2005.10.016","article-title":"Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection","volume":"135","author":"Sriveny","year":"2006","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0170","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1053\/rvsc.2000.0425","article-title":"Cathepsin L proteinases as vaccines against infection with Fasciola hepatica (liver fluke) in ruminants","volume":"70","author":"Mulcahy","year":"2001","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0175","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.vetpar.2008.10.099","article-title":"Vaccination against fasciolosis by a multivalent vaccine of stage-specific antigens","volume":"160","author":"Jayaraj","year":"2009","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0180","doi-asserted-by":"crossref","first-page":"5551","DOI":"10.1016\/j.vaccine.2010.06.039","article-title":"Protection of cattle against a natural infection of Fasciola hepatica by vaccination with recombinant cathepsin L1 (rFhCL1)","volume":"28","author":"Golden","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0185","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.pt.2010.06.001","article-title":"Cathepsin B proteases of flukes: the key to facilitating parasite control?","volume":"26","author":"Smooker","year":"2010","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0190","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.vetpar.2007.03.023","article-title":"Vaccine potential of inclusion bodies containing cysteine proteinase of Fasciola hepatica in calves and lambs experimentally challenged with metacercariae of the fluke","volume":"147","author":"Wedrychowicz","year":"2007","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0195","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1017\/S003118200800471X","article-title":"Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library","volume":"135","author":"Villa-Mancera","year":"2008","journal-title":"Parasitology"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0200","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1111\/j.1365-2249.2009.04064.x","article-title":"The therapeutic potential of the filarial nematode-derived immunomodulator ES-62 in inflammatory disease","volume":"159","author":"Harnett","year":"2010","journal-title":"Clin Exp Immunol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0205","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1046\/j.0141-9838.2001.00443.x","article-title":"Passive protection against fasciolosis in mice by immunization with a monoclonal antibody (ES-78 MoAb)","volume":"24","author":"Marcet","year":"2002","journal-title":"Parasite Immunol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0210","doi-asserted-by":"crossref","first-page":"55","DOI":"10.2307\/3284530","article-title":"Partial characterization of the epitope on excretory\u2013secretory products of Fasciola hepatica recognized by monoclonal antibody ES78","volume":"84","author":"D\u00edaz","year":"1998","journal-title":"J Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0215","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1007\/BF00936455","article-title":"Immunocytochemical localization of a cysteine protease in adult worms of the liver fluke Fasciola sp.","volume":"78","author":"Yamasaki","year":"1992","journal-title":"Parasitol Res"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0220","doi-asserted-by":"crossref","first-page":"17038","DOI":"10.1074\/jbc.M308831200","article-title":"Cathepsin L1, the major protease involved in liver fluke (Fasciola hepatica) virulence: propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells","volume":"279","author":"Collins","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0225","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.exppara.2010.03.003","article-title":"Fasciola gigantica: histology of the digestive tract and the expression of cathepsin L","volume":"125","author":"Meemon","year":"2010","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0230","series-title":"Fasciolosis","first-page":"341","article-title":"Immunology of Fasciola hepatica infection","author":"Mulcahy","year":"1999"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0235","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1046\/j.1365-3024.2001.00411.x","article-title":"Fasciola hepatica cathepsin L cysteine proteinase suppresses Bordetella pertussis-specific interferon-gamma production in vivo","volume":"23","author":"O\u2019Neill","year":"2001","journal-title":"Parasite Immunol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0240","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.tibs.2008.09.001","article-title":"Helminth pathogen cathepsin proteases: it's a family affair","volume":"33","author":"Robinson","year":"2008","journal-title":"Trends Biochem Sci"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0245","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1016\/j.molimm.2008.09.028","article-title":"Epitope characterization of a supramolecular protein assembly with a collection of monoclonal antibodies: the case of casein micelle","volume":"46","author":"Johansson","year":"2009","journal-title":"Mol Immunol"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0250","doi-asserted-by":"crossref","first-page":"9896","DOI":"10.1074\/jbc.M708521200","article-title":"Structural and functional relationships in the virulence-associated cathepsin L proteases of the parasitic liver fluke Fasciola hepatica","volume":"283","author":"Stack","year":"2008","journal-title":"J Biol Chem"},{"key":"10.1016\/j.molbiopara.2011.06.003_bib0255","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1042\/bj2990781","article-title":"The secreted cathepsin L-like proteinases of the trematode, Fasciola hepatica, contain 3-hydroxyproline residues","volume":"299","author":"Wijffels","year":"1994","journal-title":"Biochem J"}],"container-title":["Molecular and Biochemical Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166685111001587?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166685111001587?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T07:07:53Z","timestamp":1712560073000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166685111001587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,10]]}},"alternative-id":["S0166685111001587"],"URL":"https:\/\/doi.org\/10.1016\/j.molbiopara.2011.06.003","relation":{},"ISSN":["0166-6851"],"issn-type":[{"value":"0166-6851","type":"print"}],"subject":[],"published":{"date-parts":[[2011,10]]}}}