{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T06:01:59Z","timestamp":1774936919780,"version":"3.50.1"},"reference-count":59,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo","award":["2012\/05532-8"],"award-info":[{"award-number":["2012\/05532-8"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo","award":["2012\/14223-9"],"award-info":[{"award-number":["2012\/14223-9"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo","award":["2013\/20715-4"],"award-info":[{"award-number":["2013\/20715-4"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo","award":["2012\/23730-1"],"award-info":[{"award-number":["2012\/23730-1"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["474402\/2013-4"],"award-info":[{"award-number":["474402\/2013-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["140636\/2013-7"],"award-info":[{"award-number":["140636\/2013-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["134013\/2018-8"],"award-info":[{"award-number":["134013\/2018-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","award":["232251\/2014-2"],"award-info":[{"award-number":["232251\/2014-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Biochemical Parasitology"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1016\/j.molbiopara.2019.02.005","type":"journal-article","created":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T12:03:35Z","timestamp":1550145815000},"page":"24-34","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["In vitro and in vivo characterization of the multiple isoforms of Schistosoma mansoni hypoxanthine-guanine phosphoribosyltransferases"],"prefix":"10.1016","volume":"229","author":[{"given":"Larissa","family":"Romanello","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Eliza","family":"Zeraik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4562-0989","authenticated-orcid":false,"given":"Adriano","family":"de Freitas Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Roberta","family":"Torini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise E.","family":"Bird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne E.","family":"Nettleship","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather","family":"Rada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamini","family":"Reddivari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3705-2993","authenticated-orcid":false,"given":"Ray J.","family":"Owens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9398-2941","authenticated-orcid":false,"given":"Vitor Hugo Balasco","family":"Serr\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"DeMarco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Brand\u00e3o-Neto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto D'Muniz","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.molbiopara.2019.02.005_bib0005","series-title":"Report on Schistosomiasis","author":"WHO","year":"2014"},{"issue":"5","key":"10.1016\/j.molbiopara.2019.02.005_bib0010","doi-asserted-by":"crossref","first-page":"e102","DOI":"10.1371\/journal.pmed.0030102","article-title":"Incorporating a rapid-impact package for neglected tropical diseases with programs for HIV\/AIDS, tuberculosis, and malaria","volume":"3","author":"Hotez","year":"2006","journal-title":"PLoS Med."},{"issue":"2\u20133","key":"10.1016\/j.molbiopara.2019.02.005_bib0015","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0001-706X(03)00029-9","article-title":"Quantification of clinical morbidity associated with schistosome infection in sub-Saharan Africa","volume":"86","author":"van der Werf","year":"2003","journal-title":"Acta Trop."},{"issue":"4","key":"10.1016\/j.molbiopara.2019.02.005_bib0020","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.cbpa.2007.05.031","article-title":"Chemotherapy of schistosomiasis: present and future","volume":"11","author":"Caffrey","year":"2007","journal-title":"Curr. Opin. Chem. Biol."},{"issue":"6","key":"10.1016\/j.molbiopara.2019.02.005_bib0025","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1097\/01.qco.0000247591.13671.6a","article-title":"Schistosomiasis: challenges for control, treatment and drug resistance","volume":"19","author":"Fenwick","year":"2006","journal-title":"Curr. Opin. Infect. Dis."},{"issue":"3","key":"10.1016\/j.molbiopara.2019.02.005_bib0030","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/0006-2952(74)90630-3","article-title":"Pathways of nucleotide metabolism in schistosoma mansoni. V. Adenosine cleavage enzyme and effects of purine analogues on adenosine metabolism in vitro","volume":"23","author":"Crabtree","year":"1974","journal-title":"Biochem. Pharmacol."},{"issue":"20","key":"10.1016\/j.molbiopara.2019.02.005_bib0035","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1016\/0006-2952(77)90158-7","article-title":"Pathways of nucleotide metabolism in Schistosoma mansoni\u2014VII. Inhibition of adenine and guanine nucleotide synthesis by purine analogs in intact worms","volume":"26","author":"Senft","year":"1977","journal-title":"Biochem. Pharmacol."},{"issue":"3","key":"10.1016\/j.molbiopara.2019.02.005_bib0040","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0163-7258(83)90031-1","article-title":"Purine metabolism in the schistosomes: potential targets for chemotherapy","volume":"20","author":"Senft","year":"1983","journal-title":"Pharmacol. Ther."},{"issue":"4","key":"10.1016\/j.molbiopara.2019.02.005_bib0045","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/0006-2952(73)90286-4","article-title":"Pathways of nucleotide metabolism in Schistosoma mansoni. 3. Identification of enzymes in cell-free extracts","volume":"22","author":"Senft","year":"1973","journal-title":"Biochem. Pharmacol."},{"issue":"4","key":"10.1016\/j.molbiopara.2019.02.005_bib0050","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/0006-2952(73)90285-2","article-title":"Pathways of nucleotide metabolism in Schistosoma mansoni. II. Disposition of adenosine by whole worms","volume":"22","author":"Senft","year":"1973","journal-title":"Biochem. Pharmacol."},{"issue":"4","key":"10.1016\/j.molbiopara.2019.02.005_bib0055","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/0006-2952(73)90287-6","article-title":"Pathways of nucleotide metabolism in Schistosoma mansoni. IV. Incorporation of adenosine analogs in vitro","volume":"22","author":"Stegman","year":"1973","journal-title":"Biochem. Pharmacol."},{"issue":"2","key":"10.1016\/j.molbiopara.2019.02.005_bib0060","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0020-7519(72)90013-6","article-title":"Purine metabolism in Schistosoma mansoni","volume":"2","author":"Senft","year":"1972","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.molbiopara.2019.02.005_bib0065","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0166-6851(84)90062-8","article-title":"Purine salvage in Schistosoma mansoni schistosomules","volume":"11","author":"Dovey","year":"1984","journal-title":"Mol. Biochem. Parasitol."},{"issue":"3","key":"10.1016\/j.molbiopara.2019.02.005_bib0070","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0006-2952(75)90226-9","article-title":"Pathways of nucleotide metabolism in Schistosoma mansoni\u2014VI adenosine phosphorylase","volume":"24","author":"Miech","year":"1975","journal-title":"Biochem. Pharmacol."},{"issue":"2","key":"10.1016\/j.molbiopara.2019.02.005_bib0075","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/S0021-9258(17)36188-4","article-title":"Purification and characterization of hypoxanthine-guanine phosphoribosyltransferase from Schistosoma mansoni. A potential target for chemotherapy","volume":"261","author":"Dovey","year":"1986","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.molbiopara.2019.02.005_bib0080","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.exppara.2007.11.017","article-title":"Schistosoma mansoni: evaluation of an RNAi-based treatment targeting HGPRTase gene","volume":"118","author":"Pereira","year":"2008","journal-title":"Exp. Parasitol."},{"issue":"1\u20132","key":"10.1016\/j.molbiopara.2019.02.005_bib0085","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/0167-4838(93)90121-7","article-title":"Comparing the human and schistosomal hypoxanthine-guanine phosphoribosyltransferases by circular dichroism","volume":"1162","author":"Yuan","year":"1993","journal-title":"Biochim. Biophys. Acta"},{"issue":"3","key":"10.1016\/j.molbiopara.2019.02.005_bib0090","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1021\/bi00118a024","article-title":"Steady-state kinetics of the schistosomal hypoxanthine-guanine phosphoribosyltransferase","volume":"31","author":"Yuan","year":"1992","journal-title":"Biochemistry"},{"issue":"14B","key":"10.1016\/j.molbiopara.2019.02.005_bib0095","doi-asserted-by":"crossref","first-page":"7087","DOI":"10.1093\/nar\/16.14.7087","article-title":"Analysis of cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) of Schistosoma mansoni; a putative target for chemotherapy","volume":"16","author":"Craig","year":"1988","journal-title":"Nucleic Acids Res."},{"issue":"9","key":"10.1016\/j.molbiopara.2019.02.005_bib0100","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0203532","article-title":"The molecular structure of Schistosoma mansoni PNP isoform 2 provides insights into the nucleoside selectivity of PNPs","volume":"13","author":"Torini","year":"2018","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.molbiopara.2019.02.005_bib0105","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.molbiopara.2012.07.003","article-title":"Structural and kinetic studies of Schistosoma mansoni adenylate kinases","volume":"185","author":"Marques Ide","year":"2012","journal-title":"Mol. Biochem. Parasitol."},{"issue":"3","key":"10.1016\/j.molbiopara.2019.02.005_bib0110","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.jmb.2005.08.045","article-title":"Structures for the potential drug target purine nucleoside phosphorylase from Schistosoma mansoni causal agent of schistosomiasis","volume":"353","author":"Pereira","year":"2005","journal-title":"J. Mol. Biol."},{"issue":"2","key":"10.1016\/j.molbiopara.2019.02.005_bib0115","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.actatropica.2010.01.010","article-title":"Crystal structure of Schistosoma purine nucleoside phosphorylase complexed with a novel monocyclic inhibitor","volume":"114","author":"Pereira","year":"2010","journal-title":"Acta Trop."},{"issue":"Pt. 1","key":"10.1016\/j.molbiopara.2019.02.005_bib0120","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1107\/S0907444909045715","article-title":"Adenosine binding to low-molecular-weight purine nucleoside phosphorylase: the structural basis for recognition based on its complex with the enzyme from Schistosoma mansoni","volume":"66","author":"Pereira","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"issue":"Pt. 1","key":"10.1016\/j.molbiopara.2019.02.005_bib0125","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1107\/S0907444912044800","article-title":"Adenosine kinase from Schistosoma mansoni: structural basis for the differential incorporation of nucleoside analogues","volume":"69","author":"Romanello","year":"2013","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.molbiopara.2019.02.005_bib0130","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.molbiopara.2017.03.006","article-title":"Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL)","volume":"214","author":"Romanello","year":"2017","journal-title":"Mol. Biochem. Parasitol."},{"issue":"12","key":"10.1016\/j.molbiopara.2019.02.005_bib0135","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005178","article-title":"Crystal structure of Schistosoma mansoni adenosine phosphorylase\/5\u2019-methylthioadenosine phosphorylase and its importance on adenosine salvage pathway","volume":"10","author":"Torini","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.molbiopara.2019.02.005_bib0140","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.molbiopara.2017.04.004","article-title":"Schistosoma mansoni displays an adenine phosphoribosyltransferase preferentially expressed in mature female gonads and vitelaria","volume":"214","author":"Zeraik","year":"2017","journal-title":"Mol. Biochem. Parasitol."},{"issue":"1","key":"10.1016\/j.molbiopara.2019.02.005_bib0145","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ymeth.2011.08.002","article-title":"High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli","volume":"55","author":"Bird","year":"2011","journal-title":"Methods"},{"issue":"6","key":"10.1016\/j.molbiopara.2019.02.005_bib0150","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkm047","article-title":"A versatile ligation-independent cloning method suitable for high-throughput expression screening applications","volume":"35","author":"Berrow","year":"2007","journal-title":"Nucleic Acids Res."},{"issue":"Pt. 7","key":"10.1016\/j.molbiopara.2019.02.005_bib0155","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1107\/S0907444913015308","article-title":"Decision making in xia2","volume":"69","author":"Winter","year":"2013","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"issue":"Pt. 4","key":"10.1016\/j.molbiopara.2019.02.005_bib0160","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","article-title":"Overview of the CCP4 suite and current developments","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"issue":"Pt 1","key":"10.1016\/j.molbiopara.2019.02.005_bib0165","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1107\/S0907444905036693","article-title":"Scaling and assessment of data quality","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"issue":"Pt. 4","key":"10.1016\/j.molbiopara.2019.02.005_bib0170","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"J. Appl. Crystallogr."},{"issue":"Pt. 11","key":"10.1016\/j.molbiopara.2019.02.005_bib0175","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1107\/S0907444902016657","article-title":"PHENIX: building new software for automated crystallographic structure determination","volume":"58","author":"Adams","year":"2002","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"issue":"Pt. 12 Pt. 1","key":"10.1016\/j.molbiopara.2019.02.005_bib0180","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"issue":"Pt. 1","key":"10.1016\/j.molbiopara.2019.02.005_bib0185","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1107\/S0907444909042073","article-title":"MolProbity: all-atom structure validation for macromolecular crystallography","volume":"66","author":"Chen","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1016\/j.molbiopara.2019.02.005_bib0190","series-title":"The PyMOL Molecular Graphics System","author":"DeLANO","year":"2002"},{"issue":"21","key":"10.1016\/j.molbiopara.2019.02.005_bib0195","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"issue":"Pt 11","key":"10.1016\/j.molbiopara.2019.02.005_bib0200","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1017\/S0031182007002995","article-title":"Patterns of gene expression in schistosomes: localization by whole mount in situ hybridization","volume":"134","author":"Dillon","year":"2007","journal-title":"Parasitology"},{"issue":"1\u20132","key":"10.1016\/j.molbiopara.2019.02.005_bib0205","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.molbiopara.2011.03.001","article-title":"Whole mount in situ hybridization methodology for Schistosoma mansoni","volume":"178","author":"Cogswell","year":"2011","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.molbiopara.2019.02.005_bib0210","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0065-3233(08)60520-3","article-title":"The anatomy and taxonomy of protein structure","volume":"34","author":"Richardson","year":"1981","journal-title":"Adv. Protein Chem."},{"issue":"4","key":"10.1016\/j.molbiopara.2019.02.005_bib0215","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/j.jmb.2003.11.012","article-title":"Interactions at the dimer interface influence the relative efficiencies for purine nucleotide synthesis and pyrophosphorolysis in a phosphoribosyltransferase","volume":"335","author":"Canyuk","year":"2004","journal-title":"J. Mol. Biol."},{"issue":"5","key":"10.1016\/j.molbiopara.2019.02.005_bib0220","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.jmb.2005.02.064","article-title":"Alternative IMP binding in feedback inhibition of hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis","volume":"348","author":"Chen","year":"2005","journal-title":"J. Mol. Biol."},{"issue":"4376","key":"10.1016\/j.molbiopara.2019.02.005_bib0225","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1126\/science.569362","article-title":"Human and mouse hypoxanthine-guanine phosphoribosyltransferase: dimers and tetramers","volume":"203","author":"Johnson","year":"1979","journal-title":"Science"},{"issue":"2","key":"10.1016\/j.molbiopara.2019.02.005_bib0230","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0092-8674(94)90301-8","article-title":"The crystal structure of human hypoxanthine-guanine phosphoribosyltransferase with bound GMP","volume":"78","author":"Eads","year":"1994","journal-title":"Cell"},{"issue":"49","key":"10.1016\/j.molbiopara.2019.02.005_bib0235","doi-asserted-by":"crossref","first-page":"17120","DOI":"10.1021\/bi9821465","article-title":"Approaching the transition state in the crystal structure of a phosphoribosyltransferase","volume":"37","author":"Focia","year":"1998","journal-title":"Biochemistry"},{"issue":"2","key":"10.1016\/j.molbiopara.2019.02.005_bib0240","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0166-6851(94)90075-2","article-title":"Molecular characterization and overexpression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma cruzi","volume":"65","author":"Allen","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"issue":"43","key":"10.1016\/j.molbiopara.2019.02.005_bib0245","doi-asserted-by":"crossref","first-page":"15066","DOI":"10.1021\/bi981052s","article-title":"A 1.4 A crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi","volume":"37","author":"Focia","year":"1998","journal-title":"Biochemistry"},{"issue":"7","key":"10.1016\/j.molbiopara.2019.02.005_bib0250","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1110\/ps.0201002","article-title":"Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase","volume":"11","author":"Guddat","year":"2002","journal-title":"Protein Sci."},{"key":"10.1016\/j.molbiopara.2019.02.005_bib0255","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1472-6807-7-59","article-title":"Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase","volume":"7","author":"Monzani","year":"2007","journal-title":"BMC Struct. Biol."},{"issue":"22","key":"10.1016\/j.molbiopara.2019.02.005_bib0260","doi-asserted-by":"crossref","first-page":"7032","DOI":"10.1021\/bi953072p","article-title":"Crystal structure of the hypoxanthine-guanine-xanthine phosphoribosyltransferase from the protozoan parasite Tritrichomonas foetus","volume":"35","author":"Somoza","year":"1996","journal-title":"Biochemistry"},{"issue":"1","key":"10.1016\/j.molbiopara.2019.02.005_bib0265","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1073\/pnas.012399599","article-title":"The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase","volume":"99","author":"Schumacher","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"9","key":"10.1016\/j.molbiopara.2019.02.005_bib0270","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1021\/bi001195q","article-title":"The role for an invariant aspartic acid in hypoxanthine phosphoribosyltransferases is examined using saturation mutagenesis, functional analysis, and X-ray crystallography","volume":"40","author":"Canyuk","year":"2001","journal-title":"Biochemistry"},{"issue":"6","key":"10.1016\/j.molbiopara.2019.02.005_bib0275","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/9376","article-title":"The 2.0 A structure of human hypoxanthine-guanine phosphoribosyltransferase in complex with a transition-state analog inhibitor","volume":"6","author":"Shi","year":"1999","journal-title":"Nat. Struct. Biol."},{"issue":"5","key":"10.1016\/j.molbiopara.2019.02.005_bib0280","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1110\/ps.8.5.1023","article-title":"Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding","volume":"8","author":"Balendiran","year":"1999","journal-title":"Protein Sci."},{"issue":"44","key":"10.1016\/j.molbiopara.2019.02.005_bib0285","doi-asserted-by":"crossref","first-page":"14485","DOI":"10.1021\/bi990507q","article-title":"Crystal structures of the Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase-GMP and -IMP complexes: comparison of purine binding interactions with the XMP complex","volume":"38","author":"Heroux","year":"1999","journal-title":"Biochemistry"},{"issue":"1\u20132","key":"10.1016\/j.molbiopara.2019.02.005_bib0290","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.exppara.2004.01.003","article-title":"Schistosoma mansoni: sex-specific modulation of parasite growth by host immune signals","volume":"106","author":"Hernandez","year":"2004","journal-title":"Exp. Parasitol."},{"issue":"8","key":"10.1016\/j.molbiopara.2019.02.005_bib0295","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","article-title":"MRBAYES: Bayesian inference of phylogenetic trees","volume":"17","author":"Huelsenbeck","year":"2001","journal-title":"Bioinformatics"}],"container-title":["Molecular and Biochemical Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166685118302433?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0166685118302433?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T15:02:56Z","timestamp":1759071776000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0166685118302433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":59,"alternative-id":["S0166685118302433"],"URL":"https:\/\/doi.org\/10.1016\/j.molbiopara.2019.02.005","relation":{},"ISSN":["0166-6851"],"issn-type":[{"value":"0166-6851","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In vitro and in vivo characterization of the multiple isoforms of Schistosoma mansoni hypoxanthine-guanine phosphoribosyltransferases","name":"articletitle","label":"Article Title"},{"value":"Molecular and Biochemical Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molbiopara.2019.02.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}