{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T12:15:54Z","timestamp":1792239354903,"version":"4.4.0"},"reference-count":30,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":1354,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["0735252N"],"award-info":[{"award-number":["0735252N"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Cell"],"published-print":{"date-parts":[[2009,11]]},"DOI":"10.1016\/j.molcel.2009.10.025","type":"journal-article","created":{"date-parts":[[2009,11,13]],"date-time":"2009-11-13T05:06:59Z","timestamp":1258088819000},"page":"525-535","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"title":["A Reciprocal Interdependence between Nck and PI(4,5)P2 Promotes Localized N-WASp-Mediated Actin Polymerization in Living Cells"],"prefix":"10.1016","volume":"36","author":[{"given":"Gonzalo M.","family":"Rivera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Vasilescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venizelos","family":"Papayannopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendell A.","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce J.","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.molcel.2009.10.025_bib1","doi-asserted-by":"crossref","first-page":"L3","DOI":"10.1126\/stke.2002.125.pl3","article-title":"Visualizing cellular phosphoinositide pools with GFP-fused protein-modules","volume":"2002","author":"Balla","year":"2002","journal-title":"Sci. STKE"},{"key":"10.1016\/j.molcel.2009.10.025_bib2","doi-asserted-by":"crossref","first-page":"37771","DOI":"10.1074\/jbc.M204145200","article-title":"Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2","volume":"277","author":"Benesch","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.molcel.2009.10.025_bib3","doi-asserted-by":"crossref","first-page":"4586","DOI":"10.1128\/MCB.23.13.4586-4597.2003","article-title":"The murine Nck SH2\/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin network","volume":"23","author":"Bladt","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib4","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1083\/jcb.200306032","article-title":"Clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly","volume":"164","author":"Campellone","year":"2004","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib5","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/nature05185","article-title":"Phosphoinositides in cell regulation and membrane dynamics","volume":"443","author":"Di Paolo","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2009.10.025_bib6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0960-9822(99)80020-7","article-title":"Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella","volume":"9","author":"Frischknecht","year":"1999","journal-title":"Curr. Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib7","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1038\/44860","article-title":"Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling","volume":"401","author":"Frischknecht","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2009.10.025_bib8","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1038\/ncb0901-856","article-title":"Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells","volume":"3","author":"Gruenheid","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib9","doi-asserted-by":"crossref","first-page":"15376","DOI":"10.1074\/jbc.M601239200","article-title":"Phosphatidylinositol 5-kinase stimulates apical biosynthetic delivery via an Arp2\/3-dependent mechanism","volume":"281","author":"Guerriero","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.molcel.2009.10.025_bib10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/10409230601162752","article-title":"A conspicuous connection: structure defines function for the phosphatidylinositol-phosphate kinase family","volume":"42","author":"Heck","year":"2007","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib11","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/35004513","article-title":"Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein","volume":"404","author":"Kim","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2009.10.025_bib12","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1083\/jcb.149.7.1455","article-title":"GAP43, MARCKS, and CAP23 modulate PI(4,5)P(2) at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism","volume":"149","author":"Laux","year":"2000","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib13","doi-asserted-by":"crossref","first-page":"6403","DOI":"10.1038\/sj.onc.1204782","article-title":"Nck\/Dock: an adapter between cell surface receptors and the actin cytoskeleton","volume":"20","author":"Li","year":"2001","journal-title":"Oncogene"},{"key":"10.1016\/j.molcel.2009.10.025_bib14","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1083\/jcb.140.5.1125","article-title":"Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts","volume":"140","author":"Ma","year":"1998","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib15","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/35017080","article-title":"A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization","volume":"2","author":"Moreau","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib16","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1083\/jcb.200801042","article-title":"Sequential signals toward podosome formation in NIH-src cells","volume":"182","author":"Oikawa","year":"2008","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib17","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1073\/pnas.012607899","article-title":"The large GTPase dynamin regulates actin comet formation and movement in living cells","volume":"99","author":"Orth","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2009.10.025_bib18","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.molcel.2008.10.012","article-title":"Hierarchical regulation of WASP\/WAVE proteins","volume":"32","author":"Padrick","year":"2008","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2009.10.025_bib19","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.molcel.2004.11.054","article-title":"A Polybasic Motif Allows N-WASP to Act as a Sensor of PIP(2) Density","volume":"17","author":"Papayannopoulos","year":"2005","journal-title":"Mol. Cell"},{"key":"10.1016\/j.molcel.2009.10.025_bib20","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0092-8674(03)01077-8","article-title":"Specificity in signal transduction: from phosphotyrosine-SH2 domain interactions to complex cellular systems","volume":"116","author":"Pawson","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.molcel.2009.10.025_bib21","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1126\/science.290.5492.801","article-title":"Integration of multiple signals through cooperative regulation of the N-WASP-Arp2\/3 complex","volume":"290","author":"Prehoda","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.molcel.2009.10.025_bib22","doi-asserted-by":"crossref","first-page":"80","DOI":"10.7150\/ijbs.1.80","article-title":"Dissecting Nck\/Dock signaling pathways in Drosophila visual system","volume":"1","author":"Rao","year":"2005","journal-title":"Int. J. Biol. Sci."},{"key":"10.1016\/j.molcel.2009.10.025_bib23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.cub.2003.12.033","article-title":"Inducible clustering of membrane-targeted SH3 domains of the adaptor protein Nck triggers localized actin polymerization","volume":"14","author":"Rivera","year":"2004","journal-title":"Curr. Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib24","doi-asserted-by":"crossref","first-page":"26448","DOI":"10.1074\/jbc.M103856200","article-title":"Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2\/3 pathway","volume":"276","author":"Rohatgi","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.molcel.2009.10.025_bib25","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0960-9822(00)00384-5","article-title":"Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2\/3","volume":"10","author":"Rozelle","year":"2000","journal-title":"Curr. Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib26","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/nature07170","article-title":"The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency","volume":"454","author":"Sallee","year":"2008","journal-title":"Nature"},{"key":"10.1016\/j.molcel.2009.10.025_bib27","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1038\/ncb1001-897","article-title":"N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility","volume":"3","author":"Snapper","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib28","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrm2069","article-title":"The WASP-WAVE protein network: connecting the membrane to the cytoskeleton","volume":"8","author":"Takenawa","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.molcel.2009.10.025_bib29","doi-asserted-by":"crossref","first-page":"11933","DOI":"10.1073\/pnas.0701077104","article-title":"Src phosphorylation of cortactin enhances actin assembly","volume":"104","author":"Tehrani","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.molcel.2009.10.025_bib30","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1083\/jcb.200407076","article-title":"Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2\/3 complex pathway and cofilin","volume":"168","author":"Yamaguchi","year":"2005","journal-title":"J. Cell Biol."}],"container-title":["Molecular Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276509007904?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1097276509007904?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:09:55Z","timestamp":1761091795000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1097276509007904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["S1097276509007904"],"URL":"https:\/\/doi.org\/10.1016\/j.molcel.2009.10.025","relation":{},"ISSN":["1097-2765"],"issn-type":[{"value":"1097-2765","type":"print"}],"subject":[],"published":{"date-parts":[[2009,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Reciprocal Interdependence between Nck and PI(4,5)P2 Promotes Localized N-WASp-Mediated Actin Polymerization in Living Cells","name":"articletitle","label":"Article Title"},{"value":"Molecular Cell","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.molcel.2009.10.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2009 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}